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Association of Superoxide Dismutase, Glutathione Peroxidase, Catalse, and Xanthine Oxidase with Incidence of Bladder Cancer
Issue:
Volume 3, Issue 2, March 2015
Pages:
17-27
Received:
6 March 2015
Accepted:
19 March 2015
Published:
23 March 2015
Abstract: Background: Emerging evidence indicates a potential role of anti-oxidant enzymes in the prevention of bladder cancer. Aims: This study assessed the correlation between the anti-oxidative enzymes with bladder cancer incidence. The present study was aimed to estimate the levels of superoxide dismutase, glutathione peroxidase, catalase and xanthine oxidase in patients to perform a comprehensive analysis of bladder cancer antioxidant capacity. These parameters were correlated with other confounding factors age, gender, smoking, grade, stage, and tumor size and find out the correlation between all studied parameters to clarify the potential link between antioxidant enzymes expression and disease progression or metastatic dissemination. Method: This case control study was done at College of Pharmacy/Hawler Medical University in period between 15/4/2013 and 15/ 4/2014 on 50 newly diagnosed patients with bladder cancer of both genders (48 men and 2 women) with a mean age 65.12 ±10.23 years, and an equal number of matched gender–age (47 men and 3 women ) apparently healthy adults were also enrolled in this study with a mean age 62 ±9.593 years. The antioxidant enzymes were measured using high pressure liquid chromatography. Data was analyzed using Statistical Package for Social Scientists (SPSS) version 18.0. Results: There were a significant reduction in the serum superoxide dismutase, glutathione peroxidase, catalase levels and a significant elevation in the serum xanthine oxidase level, there were no significant age differences between all age categories regarding all studied parameters except, xanthine oxidase levels, there were a significant differences between two age categories 60-69 and 70, p=0.023, there were significant differences between two age categories ˂ 60 and 60-69 regarding tumor size p= 0.042, there were no significant effect of smoking, grade and stage on the serum studied parameters levels. There were no signification correlation coefficient between all studied parameters. Conclusions: These findings supported the concept that significant reduction in the serum super oxide dismutase, glutathione peroxidase, and catalase levels accompanied with significant elevation in the serum xanthine oxidase level might be a pathogenic and prognostic factors in bladder cancer. This investigation therefore focused on the measurement of variables indicative of imbalances in oxidative and anti-oxidative status. Variations in antioxidant enzyme activities might be a potentially important finding as an additional diagnostic biochemical tool for BC .These findings suggest possible use of antioxidant supplementation as prophylactic agents for prevention and treatment of bladder cancer.
Abstract: Background: Emerging evidence indicates a potential role of anti-oxidant enzymes in the prevention of bladder cancer. Aims: This study assessed the correlation between the anti-oxidative enzymes with bladder cancer incidence. The present study was aimed to estimate the levels of superoxide dismutase, glutathione peroxidase, catalase and xanthine ox...
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Clinical Utility of Altered Expressions of P53,Vascular Endothelial Growth Factor and Survivin in Patients with Bladder Cancer
Ahmed Nabeel Ahmed,
Shatha Rouf Moustafa
Issue:
Volume 3, Issue 2, March 2015
Pages:
28-41
Received:
20 March 2015
Accepted:
28 March 2015
Published:
31 March 2015
Abstract: Backgroundand Objectives: Bladder cancer is considered a major problem world wide. Aberrant P53, mutant vascular endothelial growth factor and deregulated expression of survivin were used as biochemical markers for investigation, prognosis and follow up. Aims: This study was conducted to assess the prognostic impact of altered expressions of these parameters in Erbil population with bladder cancer and correlated with other confounding factors age, gender and smoking effects, these factors also correlated with histopathologic characteristics such as grade and stage. Patients and Methods: This prospective study enrolled 50 newly diagnosed patients with bladder cancer, in addition 50 apparently healthy adults age – sex matched were also involved in this study. Serum parameters levels were measured using enzyme linked immunosorbent assay. Patients had these general criteria, newly discovered cases, no deep x-ray therapy, no chemotherapy, no hormonal therapy with histologic and cytologic confirmation of bladder cancer. Statistical Study: Data were analyzed using SPSS v. 18. Results: The mean serum P53,vascular endothelial growth factor and survivin levels in patient and control groups were P53= 1.682 ±0.665, 1.192 ± 0.284, vascular endothelial growth factor = 5.296 ±2.8, 2.000 ±0.704 and survivin = 5.468±0.715, 4.240±0.656 respectively. The statistical analysis revealed that, serum parameters levels were significantly increased in patients as compared with control group p< 0.001. Conclusion: Data revealed for the first time the relation between altered expressions of interested parameters in combination pattern with the incidence of bladder cancer in Erbil population, which has not previously reported in this region.This study tested the hypothesis and supported the concept that higher serum levels of these parameters might be a pathogenic and prognostic factors and a markers of tumor aggressiveness in bladder cancer. Early diagnosis is necessary for maximizing the rate of therapy. The gold standard care for the investigating of bladder cancer is cystoscopy which detects tumors accurately but it is invasive, expensive, and represents a high burden to the patients, and also, small papillary and flat-growing cancer may be missed. Regular cystoscopic examinations performed for monitoring the patients, because the recurrent rate is high. Therefore, this study was conducted to investigate the possibilities for replacing cystoscopy with more accurate, safe, no expensive, noninvasive diagnostic test and to recognize a new approach for providing opportunities for early detection which is an important goal to speed up the therapy of patients.
Abstract: Backgroundand Objectives: Bladder cancer is considered a major problem world wide. Aberrant P53, mutant vascular endothelial growth factor and deregulated expression of survivin were used as biochemical markers for investigation, prognosis and follow up. Aims: This study was conducted to assess the prognostic impact of altered expressions of these ...
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Osteopontin Gene Expression is Inversely Regulate 5-Fluorouracil Drug Resistance in Colon Cancer
Go Nakajima,
Kazuhiko Hayashi,
Masakazu Yamamoto
Issue:
Volume 3, Issue 2, March 2015
Pages:
42-46
Received:
28 March 2015
Accepted:
15 April 2015
Published:
27 April 2015
Abstract: Background: Resistance to chemotherapeutic drugs complicates the treatment of cancer patients. The extracellular matrix protein osteopontin (OPN) plays multiple roles in the proliferation and metastasis of cancer cells. We therefore attempted to determine whether OPN expression correlated with drug resistance. Methods: OPN expression in the HCT 116 colon cancer cell line was inhibited by an OPN-short interfering RNA (siRNA). We determined the cytotoxic effect (IC50) of 5-fluorouracil (5FU) on these cells. Patients with recurrence or colorectal cancer or harbored residual tumor cells were treated with S-1-based chemotherapy. The levels of OPN mRNA expression in the tumors were determined and compared with the patients’ responses to chemotherapy. Results: The IC50 of 5FU for HCT 116 cells transfected with the OPN-siRNA was 32.9 μM. In contrast, the IC50 values for cells transfected with a negative control siRNA, mock-transfected cells, or untreated cells were 6.58 μM, 7.08 μM, and 6.76 μM, respectively (P<0.001). The level of OPN mRNA expression in the S-1 non-responder group was significantly lower than that of the responder group (P = 0.0387). Survival analysis revealed no significant difference between the responder and non-responder groups (P = 0.8737). Conclusions: The level of OPN expression plays a role in the resistance of colorectal tumor cells to 5FU.
Abstract: Background: Resistance to chemotherapeutic drugs complicates the treatment of cancer patients. The extracellular matrix protein osteopontin (OPN) plays multiple roles in the proliferation and metastasis of cancer cells. We therefore attempted to determine whether OPN expression correlated with drug resistance. Methods: OPN expression in the HCT 116...
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