Effect of Biofield Energy Healing Based Herbomineral Formulation on Pro-inflammatory Cytokines Expression in Biofield Treated Mouse Splenocyte Cells: Impact of the Trivedi Effect®
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Barry Dean Wellborn,
Deborah Lea Smith,
Dezi Ann Koster,
Elizabeth Patric,
Jagdish Singh,
Kathleen Starr Vagt,
Krista Joanne Callas,
Olga Mirgalijeva,
Sambhu Charan Mondal,
Snehasis Jana
Issue:
Volume 4, Issue 6, November 2016
Pages:
74-83
Received:
24 November 2016
Accepted:
2 December 2016
Published:
20 December 2016
DOI:
10.11648/j.ajbio.20160406.11
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Abstract: A proprietary herbomineral formulation was formulated with four ingredients; a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in splenocyte cells, which were isolated from Biofield Treated mice. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. The splenocyte cells were treated with the test formulation at concentrations ranges from 0.00001053 to 10.53 µg/mL and analyzed after 48 hours of treatment by MTT assay. The cell viability data showed safe concentrations up to 1.053 µg/mL with viability ranges from 69.22% to 123.88% in the test formulation groups. The expression of TNF-α was decreased by 4.82% at 1.053 µg/mL in the Biofield Energy Treated test formulation compared with the vehicle control. The level of TNF-α was significantly decreased by 2.02%, 4.92%, and 18.78% at 0.00001053, 0.001053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation group as compared to the untreated test formulation. The expression of IL-1β was significantly reduced by 83.65%, 92.15%, 27.30%, and 41.88% at 0.00001053, 0.0001053, 0.001053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the vehicle control. The Biofield Treated test formulation showed significant reduction of IL-1β by 17.26%, 92.61% (p≤0.001), 34.62% (p≤0.05), and 16.13% at 0.00001053, 0.0001053, 0.001053, and 1.053 µg/mL, respectively compared with the untreated test formulation. Additionally, the expression of chemokine MIP-1α was significantly reduced by 17.03%, 10.99%, 22.33%, 24.21%, 21.61%, and 30.67% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively in the Biofield Treated test formulation compared with the vehicle control. The MIP-1α expression was significantly reduced by 19.32% and 12.56% at 0.01053 and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared with the untreated test formulation. The overall results demonstrated that the Biofield Energy Treated test formulation significantly down-regulated the expression of TNF-α, IL-1β, and MIP-1α in the Biofield Treated mice splenocyte cells compared to the untreated test formulation. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
Abstract: A proprietary herbomineral formulation was formulated with four ingredients; a mixture of the minerals (zinc, magnesium, and selenium) and the herbal root extract ashwagandha. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation in splenocyte cells, whic...
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Combining Ability and Heterosis in Maize (Zea mays L.)
Mohammad Quamrul Islam Matin,
Md. Golam Rasul,
A. K. M. Aminul Islam,
M. A. Khaleque Mian,
Nasrin Akter Ivy,
Jalal Uddin Ahmed
Issue:
Volume 4, Issue 6, November 2016
Pages:
84-90
Received:
15 November 2016
Accepted:
28 November 2016
Published:
7 January 2017
DOI:
10.11648/j.ajbio.20160406.12
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Abstract: Combining ability for growth parameter and yield components were evaluated in a 7×7 diallel fashion in maize at the research farm of Plant Breeding Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur during Rabi season 2013-14 to determine the general combining ability and specific combining ability of parents and the crosses. Significant general and specific combining ability variances were observed for some of the characters. The overall study of gca effects suggested that parent WL3 was significant for general combiner for yield, WL2 and WL3for earliness and WL1 for short stature. These parents could be used in future breeding programme to improve maize yield with desirable traits. The good combiner parents for different traits could be used in hybridization to improve yield as well as with desirable traits as donor parents for the accumulation of favorable genes. For yield improvement in maize both additive and non additive genes should be exploited through a suitable breeding method. However, the crosses WL1×WL6, WL1×WL7, WL4×WL5, WL6×WL7showed high sca effect for kernel yield. The significant positive sca of crosses could be used for commercial variety development after verifying them. The maximum heterosis was recorded in WL1×WL6 (15.60**%) when BHM 7 used as check and heterosis ranged from -56.59 to 15.60%. Only one hybrid WL2×WL7 recorded negative heterosis against BHM 7 for days to maturity.
Abstract: Combining ability for growth parameter and yield components were evaluated in a 7×7 diallel fashion in maize at the research farm of Plant Breeding Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur during Rabi season 2013-14 to determine the general combining ability and specific combining ability of parents and the cr...
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