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A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis
Hrachya Garegin Hovhannisyan,
Gohar Gagik Grigoryan
Issue:
Volume 2, Issue 3, May 2014
Pages:
84-88
Received:
7 April 2014
Accepted:
15 April 2014
Published:
30 April 2014
Abstract: Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this study is creation of symbiotic association of probiotic bacteria (SAPB) with high colonization/recolonization properties for rapid response to vaginal acute and chronic challenges. It is expected that LABs symbiotic association will be more beneficial for vaginal colonization/recoclonization, because the lactococci are growth well at elevate pH 9.0-5.0 and by reducing of vaginal pH promote the growth of lactobacilli, adapted to low pH 6.5-3.5. In this study used lactobacilli biocompatible with cocci possessing high antimicrobial activity from laboratory LABs collection, isolated earlier from 20-45 years old healthy Ar-menian woman volunteers (n=40). And it is the first time that lactococci were used as probiotic in vaginal coloniza-tion/recolonization practice. The symbiotic association of Lactobacillus plantarum (L. plantarum) GH 202 and Lactococcus lactis (L. lactis) GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans (C. albicans). The SAPB growth rate is more intensive and biomass accumulation is higher, than the mono cultures. It is sustainable at different pH conditions of growth and during multiple subculturing imitating up and down changes of vaginal pH. The SAPB strains have high hydrophobicity evaluated by MATS test and high coaggregation properties essential for increasing their colonization potential, when they used in mixed culture.
Abstract: Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this stu...
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Serum Cystatin C an Early Indicator of Renal Function Decline in Type 2 Diabetes
Hany S. Elbarbary,
Nabil A. El-Kafrawy,
Ahmed A. Shoaib,
Samar M. Kamal El-deen
Issue:
Volume 2, Issue 3, May 2014
Pages:
89-94
Received:
29 March 2014
Accepted:
20 April 2014
Published:
30 April 2014
Abstract: Objectives: evaluation of cystatin C level in the serum as a predictor of early renal impairment in type 2 diabetic patients. Background: the glomerular filtration rate (GFR) is often estimated from plasma creatinine. Several studies have shown that cystatin C (Cys C) can be used as a better marker for the early detection of renal function decline. Methods: patients were classified according to the urine albumin/creatinine ratio (ACR).Plasma samples were obtained from 20 healthy persons and from 40 patients with diabetes mellitus type 2 for determination of the level of creatinine and cystatin C. Results: There were no significant differences in age and sex between the three groups. However, There was a significant positive correlation between cystatin C and age, A/C ratio, HbA1c, FBS, 2HPP, DM duration and serum creatinine, and there was a significant negative correlation between cystatin C and glomerular filtration rate. eGFR was significantly lower in the macroalbuminuric group than in the micro-albuminuric and normo-albuminuric groups, and cystatin C showed the highest sensitivity and specificity in detecting micro and macro-albuminuria and accordingly early renal function decline in diabetic patients. Conclusion: from this study we concluded that serum cystatin C is a useful, practical, and non-invasive tool for early detection of renal impairment in the course of diabetes.
Abstract: Objectives: evaluation of cystatin C level in the serum as a predictor of early renal impairment in type 2 diabetic patients. Background: the glomerular filtration rate (GFR) is often estimated from plasma creatinine. Several studies have shown that cystatin C (Cys C) can be used as a better marker for the early detection of renal function decline....
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Male Age Influence on Male Remating and Progeny Production in Drosophila Malerkotliana
Shivakumar P.,
Krishna M. S.
Issue:
Volume 2, Issue 3, May 2014
Pages:
95-101
Received:
7 January 2014
Accepted:
28 April 2014
Published:
30 April 2014
Abstract: Reproductive success of the male is not only depends on his success in mating, but the number of females he could inseminate in a given time. The age effect on male remating and progeny production has been studied in Drosophila malerkotliana. It was found that middle aged males had a greater percentage of remating than those of young or old males. Male mated two females in an hour had copulated longer, laid a greater number of eggs and produced the greatest number of progeny than those males which mated with one female in an hour. This result found to be similar in all the male age classes studied. It was also noted that young male copulated longest in the experiment involving male mated only one female in 1 hour. Female that mated with middle aged male laid least fecundity in the experiment involving male mated only one female in 1 hour. Whereas female that mated with middle aged male had produced greater progeny in the experiment in which male mated with one/two females in 1 hour. Among the male mated with 1st and 2nd female in an hour male invested more in 1st mated female compared to 2nd mated female.
Abstract: Reproductive success of the male is not only depends on his success in mating, but the number of females he could inseminate in a given time. The age effect on male remating and progeny production has been studied in Drosophila malerkotliana. It was found that middle aged males had a greater percentage of remating than those of young or old males. ...
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Nuclear Techniques in Agriculture and Genetics
Askari Mohammad Bagher,
Askari Nahid,
Mirhabibi Mohsen,
Mirzaei Vahid
Issue:
Volume 2, Issue 3, May 2014
Pages:
102-105
Received:
22 April 2014
Accepted:
6 May 2014
Published:
20 May 2014
Abstract: These days, practically everyone is aware of the role nuclear technology has played in revolutionizing electricity generation and energy diversification. Not so well-known are the many other, non-power applications of this technology. During the past half-century, the worlds of medicine, agriculture and industry have seen significant improvements due to the harnessing of radioisotopes. And with the surfacing of new applications every day, nuclear science continues to make major contributions to the quality of our life, providing welfare and prosperity for all. Here, we’ll look at some of the contributions of nuclear technology, and why Iran can – and is trying – to benefit from it In addition to other nuclear techniques, the classical method can solve many of the problems of effective and rapid agricultural issues. One of the peaceful applications of nuclear energy in agriculture is to create a genetic mutation in plants, and increase the variety of plants for the reform, the importance of this technique on increase of yield and the amount of the medicinal and aromatic plants in ingredients has been significant. Valuable medicinal plants, according to Iran's reserves, in this article, a study of the success obtained in the use of nuclear techniques to increase yield and active constituents in some of the most important medicinal and aromatic plants will be paid.
Abstract: These days, practically everyone is aware of the role nuclear technology has played in revolutionizing electricity generation and energy diversification. Not so well-known are the many other, non-power applications of this technology. During the past half-century, the worlds of medicine, agriculture and industry have seen significant improvements d...
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Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis
Mohamed Elmogy,
Azza M. Elgendy,
Makio Takeda
Issue:
Volume 2, Issue 3, May 2014
Pages:
106-110
Received:
12 April 2014
Accepted:
28 April 2014
Published:
30 May 2014
Abstract: In the present study, the small GTP binding proteins involved in the regulatory mechanism of vitellogenin (Vg) endocytotic vesicles trafficking were detected, for the first time, in ovaries of the most basal hemimetabolus insect, Periplaneta americana during oogenesis. The ovarian GTPase activities were peaked during previtellogenic and early vitellogenic periods. Such activity coincides with vitellogenin receptors (VgRs) and clathrin early expression during these developmental periods, suggesting the importance of GTPases not only in the process of vesicle formation and fusion but also in the process of early fluid phase endocytosis. Two small peaks of activities were monitored during the late vitellogenic period (days 8 and 10), suggesting a possible role of GTPases in VgRs and clathrin recycling process. The [α32P]-GTP binding assay analysis in different tissues revealed the presence of small GTP binding proteins of molecular weights 25, 23 and 21 kDa in ovaries and head. However, a single binding signal band of 21 and 25 kDa was each detected in the fat bodies and muscles, respectively. No binding was detected in the midgut and Malpighian tubules. However, the 23 kDa protein detected was suggested as a probable cytosolic form of the 25 kDa protein. The competition assay results indicated that the small ovarian GTP binding proteins could also bind ATP, suggesting that like GTP, ATP is a regulatory nucleotide for the ovarian small proteins detected during oogenesis. The present study will pave the way for more understanding of the mechanisms that regulate Vg transport machinery in hemimetabolous insects.
Abstract: In the present study, the small GTP binding proteins involved in the regulatory mechanism of vitellogenin (Vg) endocytotic vesicles trafficking were detected, for the first time, in ovaries of the most basal hemimetabolus insect, Periplaneta americana during oogenesis. The ovarian GTPase activities were peaked during previtellogenic and early vitel...
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