Abstract: Viral infections pose significant threats to plant health, impacting agriculture and medicinal plant production. This study focuses on diagnosing viral infections in Ipomoea cairica (L.) Sweet using advanced techniques such as Transmission Electron Microscopy (TEM) and Microtomy. Phenotypic symptoms, anatomical changes, and the confirmation of Gemini virus through TEM are investigated. Transmission studies reveal multiple vectors, including Bemisia tabaci, highlighting the importance of understanding viral spread mechanisms. Host range studies demonstrate widespread susceptibility among various plant families. The research emphasizes early diagnosis and management to mitigate economic losses and preserve medicinal metabolites. Future efforts will employ PCR and DNA sequencing for precise viral strain identification and targeted management strategies. The findings underscore the importance of early diagnosis and management of viral infections to safeguard plant health and preserve essential metabolites. Future research aims to utilize PCR and DNA sequencing for precise identification and targeted management of viral strains, enabling effective disease control strategies for Ipomoea cairica (L.) Sweet and similar plant species. This study underscores the necessity for proactive measures to protect plant species like Ipomoea cairica (L.) Sweet from viral infections, ensuring sustainable agriculture and medicinal plant production.
Abstract: Viral infections pose significant threats to plant health, impacting agriculture and medicinal plant production. This study focuses on diagnosing viral infections in Ipomoea cairica (L.) Sweet using advanced techniques such as Transmission Electron Microscopy (TEM) and Microtomy. Phenotypic symptoms, anatomical changes, and the confirmation of Gemi...Show More
Abstract: Background and Purpose: Candidiasis are the most frequent fungal infection, especially in immunocompromised people such as patients living with the human immunodeficiency virus (PLHIV). Data on the fungal prevalence and antifungal resistance rate particularly in PLHIV in Cameroon are scarce. This study aimed to determine the distribution and the antifungal resistance profile of Candida spp. isolated from clinical samples of PLHIV visiting the laboratory service of the Yaoundé Central Hospital. Materials and Methods: A cross-sectional study was carried out on the PLHIV visiting the Central Hospital of Yaoundé laboratory service. Samples were collected according to the signs and symptoms recorded by the patient and inoculated onto Sabouraud + Chloramphenicol agar medium for 24 hours incubation at 35°C ± 2°C. Typical Candida colonies were subjected to a germ tube test to identify Candida albicans and the other species were identified biochemically using API Candida (BioMérieux). The antifungal susceptibility testing was carried out by the disk diffusion method and seven antifungal discs (Bioanalyse) were tested. Results: Overall, 106 unique samples were obtained from participants. The positivity rate of Candida spp. was 37.7%. Candida isolates were mostly recovered from sputum (n=15/40) followed by the oral swabs (n=10/40) and the vaginal swabs (n=08/40). Out of the 40 isolates, Candida albicans was the predominant species 57.5% followed by Candida krusei 15%, Candida glabrata and Candida guilliermondii 10% each and Candida famata 7.5%. The antifungal drug resistance profile of Candida spp. revealed the highest resistance rates to Amphotericin B (95.0%), Fluconazole (57.5%) and Nystatin (42.5%). Conversely, Clotrimazole, Miconazole and Econazole were the most effective against Candida spp. Conclusion: The high frequency of Candida spp. isolation, resistant to several commonly used antifungals among PLHIV in a hospital setting is a direct call for stakeholders, policymakers and clinicians about antifungal therapy awareness in this vulnerable population.Abstract: Background and Purpose: Candidiasis are the most frequent fungal infection, especially in immunocompromised people such as patients living with the human immunodeficiency virus (PLHIV). Data on the fungal prevalence and antifungal resistance rate particularly in PLHIV in Cameroon are scarce. This study aimed to determine the distribution and the an...Show More