Research Article
Compound Identification by HPLC-ESI-Q-TOF-MS/ MS Analysis of the Dichloromethane Fraction of Hyptis suaveolens Leaves After Extraction of the Essential Oil
Brahima Soumahoro*,
Gouegoui Serge-Pacôme Bohui,
Mabintou Kalo,
Losseyni Kanaté,
Barthélémy Attioua,
Yaya Soro
Issue:
Volume 12, Issue 1, February 2024
Pages:
1-14
Received:
17 December 2023
Accepted:
5 January 2024
Published:
1 February 2024
Abstract: Hyptis suaveolens (Lamiaceae), widespread in tropical areas of America, Asia, and Africa, is used in more than twenty-two countries for its medicinal properties. The plant's leaves contain polyphenols, tannins, sterols and terpenes, saponins, flavonoids, quinones and anthraquinones before and after extraction of the essential oil. However, no molecular structures were identified in the leaves of the plant after extraction of the essential oil. So, after hydroethanolic (70/30; v/v) maceration of 100g of powder front and after extracting essential oil taken separately, a successively fractionate extract was obtained from solvents of increasing polarities. Next, the compounds of the dichloromethane fraction after extracting essential oil have been identified by HPLC-ESI- Q-TOF -MS/ MS method. As a result, the determination of the structures of nine (9) known compounds of the Hyptis genus was carried out by dereplication on the dichloromethane fraction from the hydroalcoholic extract (70%) of the plant's leaves after extraction of the essential oil. Of these nine (09) compounds, four (04) are phenolics and the remaining five (05) are terpenoids. Five (05) of these molecules had not yet been identified in Hyptis suaveolens leaves. This study shows that Hyptis suaveolens leaves after extraction of the essential oil are new source of bioactive compounds of pharmacological interest.
Abstract: Hyptis suaveolens (Lamiaceae), widespread in tropical areas of America, Asia, and Africa, is used in more than twenty-two countries for its medicinal properties. The plant's leaves contain polyphenols, tannins, sterols and terpenes, saponins, flavonoids, quinones and anthraquinones before and after extraction of the essential oil. However, no molec...
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Research Article
Analysis by LC-Ms/Ms of Drugs Sold in the Markets of Kara and Lome
Issue:
Volume 12, Issue 1, February 2024
Pages:
15-22
Received:
29 December 2023
Accepted:
25 January 2024
Published:
1 February 2024
Abstract: The falsification of medicines is a worldwide scourge, but one that particularly affects developing countries, causing a great deal of damage, including deaths. Fake medicines are seized by customs every year. However, very few studies have looked at the qualitative and quantitative analysis of medicines found in Africa, both in pharmacies and on the markets. This study aims to analyze several drugs purchased in Togo in the cities of Kara and Lomé to ensure that they actually have the active ingredients indicated inside, which is one of the guarantees of their quality. The samples were analyzed by HPLC coupled with MS/MS which is a method of choice for the identification of molecules of interest. Analysis of the spectra obtained showed that the samples purchased on the markets (analgesics, anti-inflammatory drugs and antibiotics) contain all the active ingredients indicated on the package. This result is reassuring because 100% of the samples analyzed are of good quality. However, it will be necessary to make additional studies by adding other classes of drugs such as anti-malarials that are most affected by falsification on the African continent. A quantitative study should also be done to determine the amount of each active ingredient in the tablets studied. It will be interesting to study the excipients contained in drugs because, although they are not active principles, they have an influence on the metabolism of active molecules.
Abstract: The falsification of medicines is a worldwide scourge, but one that particularly affects developing countries, causing a great deal of damage, including deaths. Fake medicines are seized by customs every year. However, very few studies have looked at the qualitative and quantitative analysis of medicines found in Africa, both in pharmacies and on t...
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Research Article
Enzyme Urease Activity and Phytochemical Investigation of the Leaves of Baissea Mortehanii (Apocynaceae)
Ngah Lidwine*,
Essombe Malolo Fanny-Aimee,
Ngo Nyobe Caroline,
Nko’o Henri Julien,
Willifred Dongmo Tekapi Tsopgni,
Etame Loe Gisele,
Jean Duplex Wansi,
Kamdem Waffo,
Ndom Jean Claude,
Mpondo Mpondo Emmanuel
Issue:
Volume 12, Issue 1, February 2024
Pages:
23-26
Received:
16 January 2024
Accepted:
30 January 2024
Published:
21 February 2024
Abstract: The phytochemical investigation of a previously unstudied species of the genus Apocynaceae, Baissea mortehanii de Wild was undertaken and eight known secondary metabolites were isolated from leaves of this plant including one alkaloid, N-Feruloyltryptamine (1); one aromatic ester, Dibutyl phthalate (2); two flavonoids, Genistein (3) and Gerontoisoflavone A (4), four sterols, β-Sitosterol (5), Sitosterol-3-O-β-D-glucopyranoside (6), Stigmasterol (7) and Stigmasterol-3-O-β-D glucopyranoside (8). The structures of compounds were determined by means of spectroscopic methods :NMR analysis (1H and13C NMR, 1H-1H-COSY, HSQC, HMBC), spectrometric methods such as UV, IR, ESI-MS, EI, and by comparing their data with those reported in the literature. All the isolated compounds were tested for their potential to inhibit the enzyme urease. Urease activity was determined by measuring ammonia production using the indophenol method and thiourea was used as standard inhibitor of urease. Compounds 5 and 7 showed the best urease inhibition with an IC50 value 17. 2 and 18.5 µM respectively, which is higher than that of the potent inhibitor, thiourea (IC50 = 21.5 µM); Compounds 3, 4, 6 and 8 showed a good urease inhibition with an IC50 value 26.9, 29.7, 32.8 and 34.3 µM respectively; Compounds 1 and 2 showed a moderate urease inhibition with an IC50 value 49.1 and 46.8 µM respectively.
Abstract: The phytochemical investigation of a previously unstudied species of the genus Apocynaceae, Baissea mortehanii de Wild was undertaken and eight known secondary metabolites were isolated from leaves of this plant including one alkaloid, N-Feruloyltryptamine (1); one aromatic ester, Dibutyl phthalate (2); two flavonoids, Genistein (3) and Gerontoisof...
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