Chemical Composition and Physico-Chemical Analysis of Eucalyptus Globulus Leave and Oil
Abayneh Kassahun,
Gezu Feleke
Issue:
Volume 7, Issue 2, April 2019
Pages:
36-38
Received:
6 April 2019
Accepted:
17 May 2019
Published:
19 June 2019
Abstract: Eucalyptus globulus grows well in different parts of the world. More than 300 species of this genus have been shown to contain volatile oil. Essential oil contains many hundred chemical elements and this advanced mixture of compounds offers the oil its characteristic fragrance and flavor. The investigation has been carried out to know the chemical composition, Physico-Chemical properties of essential oils and Eucalyptus globulus leave grown at Ankober woreda, Ethiopia. To extract the essential oil, the fresh leaves of Eucalyptus globulus were subjected to hydro distillation for 3 hours using a Clevenger-type apparatus and the percent yield was 2.1%. Physico-chemical properties such as refractive index (1.451), relative density (0.925), optical rotation (+8o), Solubility, color and odour were determined. Physico-chemical analysis of Eucalyptus globulus leaves such as total ash, water soluble ash; acid insoluble ash, moisture content and pH were found to be (6.09), (2.18), (2.22), (35.32) and (5.43) respectively. The composition of essential oils of Eucalyptus globulus were analyzed using Gas Chromatography-Mass Spectroscopy (GC-MS). A total of twenty volatile components were identified in this work. The main components of the essential oils are Alpha-Pinene (25.55%), D-Limonene (5.687%) and Eucalyptol (55.43%).
Abstract: Eucalyptus globulus grows well in different parts of the world. More than 300 species of this genus have been shown to contain volatile oil. Essential oil contains many hundred chemical elements and this advanced mixture of compounds offers the oil its characteristic fragrance and flavor. The investigation has been carried out to know the chemical ...
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Atropisomeric Separation of PCB-95 by HPLC
Prabha Ranasinghe,
Christopher Olivares,
William Champion Jr,
Cindy Lee
Issue:
Volume 7, Issue 2, April 2019
Pages:
39-48
Received:
3 February 2019
Accepted:
20 March 2019
Published:
29 June 2019
Abstract: 2,2’,3,5’,6-Pentachlorobiphenyl (PCB-95) is an environmentally significant chiral PCB, of which enantioselective toxicity, biodegradation and chiral stability studies have been limited to date, as no commercially available enantiomers exist for PCB-95 and due to the lack of an efficient preparatory chiral separation method. A selective, sensitive, and rapid high-performance liquid chromatography with UV detection (HPLC-UV) method has been developed and validated for the chromatographic separation and quantitation of PCB-95 enantiomers. In this study, we resolved enantiomers of PCB-95 using a cellulose tris (4-methylbenzoate) Chiralcel OJ- H column. After evaluating mobile phase compositions and temperatures, optimum separation and detection were obtained with isocratic 100% n-hexane as the mobile phase, a column temperature of 20°C, a flow rate of 1 mL/min, and a detection wavelength of 280 nm. The total run time was 8 minutes. Enantiomer purity was confirmed using enantioselective gas capillary chromatography-electron capture detection. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to limit of detection, limit of quantification, precision, linearity, robustness and ruggedness.
Abstract: 2,2’,3,5’,6-Pentachlorobiphenyl (PCB-95) is an environmentally significant chiral PCB, of which enantioselective toxicity, biodegradation and chiral stability studies have been limited to date, as no commercially available enantiomers exist for PCB-95 and due to the lack of an efficient preparatory chiral separation method. A selective, sensitive, ...
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Synthesis, Characterization and In Vitro Cytotoxicity of Platinum (II) Complexes with Some Tetradentate Salen Ligands
Quang Trung Nguyen,
Quang Hai Lam,
Phuong Nam Pham Thi,
Van Tuyen Nguyen
Issue:
Volume 7, Issue 2, April 2019
Pages:
49-55
Received:
15 May 2019
Accepted:
17 June 2019
Published:
1 July 2019
Abstract: A series of platinum (II) complexes of Schiff bases derived from various salicylaldehydes with ethylenediamine were synthesized and characterized by ESI-MS, NMR and IR. The results of ESI-MS and NMR spectra data showed that the separation of hydrogen from hydoxy group and different chemical shifts of typical bondings between ligands and platinum (II) complexes evidenced for coordination of Pt (II) and tetradentate ligands. The IR spectral studies indicated the binding sites of the salen ligands with Pt (II). Their photophysical properties studied by UV-vis and luminescent spectroscopies performed the photoactivity of investigated platinum (II) complexes. The obvious effect of electron-withdrawing and – donoring substituted groups at the same position of salicyl rings and the impact of methoxy at different position of salicyl rings to their photophysical properties were studied and compared to complex 1 without substituted group. The obtained complexes were screened for their in vitro antitumour activities against KB and MCF-7 human cancer cell lines. Complex 4 showed the best bioactivity for both KB and MCF-7 human cancer cells with IC50 1.92 µM.
Abstract: A series of platinum (II) complexes of Schiff bases derived from various salicylaldehydes with ethylenediamine were synthesized and characterized by ESI-MS, NMR and IR. The results of ESI-MS and NMR spectra data showed that the separation of hydrogen from hydoxy group and different chemical shifts of typical bondings between ligands and platinum (I...
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