Antibiofilm Activity of Lactobacillus Strains
Issue:
Volume 6, Issue 5, October 2018
Pages:
77-82
Received:
30 September 2018
Accepted:
22 October 2018
Published:
15 November 2018
Abstract: The development of antibiofilm strategies is of major interest in contrasting bacterial biofilms that are a predominant microbial style of life in natural and technical ecosystems. Тhe aim of this study is to evaluate the impact of metabolites produced during the cultivation of lactobacilli in MRS broth, on the biofilm-formation activity of co-cultures Bacillus subtilis and Escherichia coli K-12 strains. For this purpose, several classical microbiological tools, in particular method for static cultivating of biofilms in 96-well polystyrene plates, and confocal laser scanning microscopy were applied. Thus, the inhibitory effect of eight Lactobacillus strains, isolated from homemade dairy products manufactured in Rodopi Mountain, Bulgaria, has been estimated. A strain-specific anti-biofilm activity of cells-free supernatants from eight exponential Lactobacillus cultures on the biofilms formed by Bacillus subtilis NBIMCC 170 and Escherichia coli K12 strain 1655 and by co-culture of B. subtilis NBIMCC 168 - E.coli K12- 1655 was observed. Lactobacillus plantarum L32 strains exhibited a good antibiofilm activity against co-cultures of Bacillus subtilis and Escherichia coli K12 strains. Data shows that the cell-free supernatants of Lactobacillus delbrueckii subsp. bulgaricus strain stimulate sporulation process in the structure of the biofilm by B.subtilis 170 and E.coli K-12 1655 strains and by B. subtilis 168 and E.coli K-12 1655 strains in comparison to Lactobacillus plantarum strains. In the structure of formed biofilms, the role of dominant species is implemented by B. subtilis strains in the presence of cell-free supernatants of Lactobacillus strains and at delution rate of cell-free supernatants of Lactobacillus plantarum L32 strains in MRS broth in the range from 1:10 to 1:1000. The data of the confocal laser scanning microscopy shows that at dilution rate of cell-free supernatant of 10-1 leads to appearance of blank optical field, the increase of metabotnite products of Lactobacillus plantarum L32 strain at dilution rate in the range of 10-2 – 10-3 creates conditions for increasing of intensity of staining by immunofluorescence days in this study. The obtained results showed that a strong anti-biofilm forming effect was obtained with Lactobacillus plantarum L32 culture in MRS broth.
Abstract: The development of antibiofilm strategies is of major interest in contrasting bacterial biofilms that are a predominant microbial style of life in natural and technical ecosystems. Тhe aim of this study is to evaluate the impact of metabolites produced during the cultivation of lactobacilli in MRS broth, on the biofilm-formation activity of co-cultur...
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A Review on Heterocyclic: Synthesis and Their Application in Medicinal Chemistry of Imidazole Moiety
Mossaraf Hossain,
Ashis Kumar Nanda
Issue:
Volume 6, Issue 5, October 2018
Pages:
83-94
Received:
18 October 2018
Accepted:
1 November 2018
Published:
28 November 2018
Abstract: In an organic chemistry, largest families of organic compounds are belongs in the heterocyclic compounds. In our daily life important of heterocyclic compounds are of very essential. It has broad range of application in medicinal chemistry and in agrochemicals products. Applications are also found in as developers, as corrosion inhibitors, sanitizers, as copolymers, antioxidants, dye stuff. There is always an important thing about an efficient methodology for synthesizing of new heterocycles moiety. Now in literature survey reveals that more than 85-95% new drugs containing heterocycles which has bright scientific insight in the biological system. In this review work, I mainly focus such type of heterocycle and their families which has main utility in medicinal chemistry. In the recent past developments of imidazole-based compounds in the wide range of medicinal chemistry such as antihypertensive, antineuropathic, antitubercular, antiviral, anti-inflammatory, antibacterial, antiobesity, antiparasitic, antifungal, antihistaminic, anticancer, and other potential medicinal agents with their broad applications in pathology and diagnostics. Derivatives of imidazole have placed a unique position in the medicinal chemistry field. The involvement of the imidazole scaffold is a key of synthetic strategy in the drug discovery system. The imidazole moiety is a part of several important naturally occurring products, including histamine, purine, nucleic acid and histidine. It is expected that this brief review could be attractive for new thoughts from academia and pharmaceutical industries to designs of more biologically active and non-toxic imidazole-based drugs. The aims of this review work to the reported imidazole derivatives with pharmaceuticals activity during the past years.
Abstract: In an organic chemistry, largest families of organic compounds are belongs in the heterocyclic compounds. In our daily life important of heterocyclic compounds are of very essential. It has broad range of application in medicinal chemistry and in agrochemicals products. Applications are also found in as developers, as corrosion inhibitors, sanitize...
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Study of Agronomic and Environmental Profile of Compost and Fine Fraction Produced and Stored in a Shed at Composting Site: ENPRO Composting Site, Lomé, Togo
Etsè Aziablé,
Edem Komi Kolédzi
Issue:
Volume 6, Issue 5, October 2018
Pages:
95-98
Received:
5 October 2018
Accepted:
8 November 2018
Published:
5 December 2018
Abstract: Composting is one of the safest ways to recycle household solid waste. This study aims at evaluating the quality of the agronomic profile of the compost and fine fraction collected on the composting platform of the NGO ENPRO in July 2014. The quality of the finished product was sought by the analysis of the agronomic and environmental profile. The results of the analyzes of the agronomic and environmental profile of compost and the fine fraction produced and stored for several months in a shed at the ENPRO composting site in Lomé, Togo show that the long lasting storage of compost on the platform (stored at least 3 months under the shed) induces a significant change in the agronomic profile: significant loss of nitrogen and significant mineralization of organic matter. The analysis of the environmental profile of the 2 compost samples and the fine fraction yielded contents of low levels of trace metal elements, very below the limit values of the French standard U44051. As a result, the compost obtained is a stable product, hygienic, rich in nutrients and organic matter. This organic matter content will allow the retention of trace metal elements. The higher this concentration, the more metals will be trapped thus decreasing their bioavailability. The risk would therefore be low in terms of phytotoxicity after soil amendment. This compost can stabilize or immobilize the solid phase metals. This compost will pave the way for agronomic promotion.
Abstract: Composting is one of the safest ways to recycle household solid waste. This study aims at evaluating the quality of the agronomic profile of the compost and fine fraction collected on the composting platform of the NGO ENPRO in July 2014. The quality of the finished product was sought by the analysis of the agronomic and environmental profile. The ...
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