Type 2 diabetes mellitus (T2DM) is a metabolic disorder caused by hyperglycemia which is the most common form of diabetes and causes impairment in insulin secretion and insulin resistance. Increased inflammatory elements such as eNOS can be associated with type 2 diabetes and depression. Therefore, the above study investigated the effect of eNOS gene expression on diabetes-related depression in patients with types 2 diabetes. This study is of case-control type. The participants of this study are those who referred to the diabetes outpatient clinic of Shariati Hospital. The present study was performed on 50 male and female patients with type 2 diabetes mellitus and depression, and 50 type 2 diabetic patients without depression as a control group. RNA extraction from blood was performed by a unique test kit and finally, gene expression was measured using quantitative Real-Time-PCR and NanoDrop spectophotometers. The expression of nitric oxide synthase gene in patients with type 2 diabetes and depression increased compared to people with type 2 diabetes without depression, which was not statistically significant (p>0.05). Regarding the increase of this enzyme in the case group, it can be said that the concentration of reactive oxygen or nitrogen species such as Superoxide (O2-), Nitric oxide (NO) and Peroxynitric acid (HNO4) can increase in conditions such as inflammation that inhibit the body’s natural defense and antioxidant activities. The present study showed that increased eNOS expression level is associated with post-diabetes depression. Therefore, it can be a good treatment goal for type 2 diabetic patients with depression.
| Published in | Pathology and Laboratory Medicine (Volume 5, Issue 2) |
| DOI | 10.11648/j.plm.20210502.15 |
| Page(s) | 43-49 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Gene Expression, eNOS, Type 2 Diabetes, Depression, Real-Time-PCR, Nano Drop Spectophotometers
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APA Style
Mahsa Khosrogardi, Mahsa Mohammad Amoli. (2021). The Relationship Between eNOS Gene Expression and Patients with Type 2 Diabetes and Depression. Pathology and Laboratory Medicine, 5(2), 43-49. https://doi.org/10.11648/j.plm.20210502.15
ACS Style
Mahsa Khosrogardi; Mahsa Mohammad Amoli. The Relationship Between eNOS Gene Expression and Patients with Type 2 Diabetes and Depression. Pathol. Lab. Med. 2021, 5(2), 43-49. doi: 10.11648/j.plm.20210502.15
AMA Style
Mahsa Khosrogardi, Mahsa Mohammad Amoli. The Relationship Between eNOS Gene Expression and Patients with Type 2 Diabetes and Depression. Pathol Lab Med. 2021;5(2):43-49. doi: 10.11648/j.plm.20210502.15
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Download@article{10.11648/j.plm.20210502.15,
author = {Mahsa Khosrogardi and Mahsa Mohammad Amoli},
title = {The Relationship Between eNOS Gene Expression and Patients with Type 2 Diabetes and Depression},
journal = {Pathology and Laboratory Medicine},
volume = {5},
number = {2},
pages = {43-49},
doi = {10.11648/j.plm.20210502.15},
url = {https://doi.org/10.11648/j.plm.20210502.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plm.20210502.15},
abstract = {Type 2 diabetes mellitus (T2DM) is a metabolic disorder caused by hyperglycemia which is the most common form of diabetes and causes impairment in insulin secretion and insulin resistance. Increased inflammatory elements such as eNOS can be associated with type 2 diabetes and depression. Therefore, the above study investigated the effect of eNOS gene expression on diabetes-related depression in patients with types 2 diabetes. This study is of case-control type. The participants of this study are those who referred to the diabetes outpatient clinic of Shariati Hospital. The present study was performed on 50 male and female patients with type 2 diabetes mellitus and depression, and 50 type 2 diabetic patients without depression as a control group. RNA extraction from blood was performed by a unique test kit and finally, gene expression was measured using quantitative Real-Time-PCR and NanoDrop spectophotometers. The expression of nitric oxide synthase gene in patients with type 2 diabetes and depression increased compared to people with type 2 diabetes without depression, which was not statistically significant (p>0.05). Regarding the increase of this enzyme in the case group, it can be said that the concentration of reactive oxygen or nitrogen species such as Superoxide (O2-), Nitric oxide (NO) and Peroxynitric acid (HNO4) can increase in conditions such as inflammation that inhibit the body’s natural defense and antioxidant activities. The present study showed that increased eNOS expression level is associated with post-diabetes depression. Therefore, it can be a good treatment goal for type 2 diabetic patients with depression.},
year = {2021}
}
TY - JOUR T1 - The Relationship Between eNOS Gene Expression and Patients with Type 2 Diabetes and Depression AU - Mahsa Khosrogardi AU - Mahsa Mohammad Amoli Y1 - 2021/11/10 PY - 2021 N1 - https://doi.org/10.11648/j.plm.20210502.15 DO - 10.11648/j.plm.20210502.15 T2 - Pathology and Laboratory Medicine JF - Pathology and Laboratory Medicine JO - Pathology and Laboratory Medicine SP - 43 EP - 49 PB - Science Publishing Group SN - 2640-4478 UR - https://doi.org/10.11648/j.plm.20210502.15 AB - Type 2 diabetes mellitus (T2DM) is a metabolic disorder caused by hyperglycemia which is the most common form of diabetes and causes impairment in insulin secretion and insulin resistance. Increased inflammatory elements such as eNOS can be associated with type 2 diabetes and depression. Therefore, the above study investigated the effect of eNOS gene expression on diabetes-related depression in patients with types 2 diabetes. This study is of case-control type. The participants of this study are those who referred to the diabetes outpatient clinic of Shariati Hospital. The present study was performed on 50 male and female patients with type 2 diabetes mellitus and depression, and 50 type 2 diabetic patients without depression as a control group. RNA extraction from blood was performed by a unique test kit and finally, gene expression was measured using quantitative Real-Time-PCR and NanoDrop spectophotometers. The expression of nitric oxide synthase gene in patients with type 2 diabetes and depression increased compared to people with type 2 diabetes without depression, which was not statistically significant (p>0.05). Regarding the increase of this enzyme in the case group, it can be said that the concentration of reactive oxygen or nitrogen species such as Superoxide (O2-), Nitric oxide (NO) and Peroxynitric acid (HNO4) can increase in conditions such as inflammation that inhibit the body’s natural defense and antioxidant activities. The present study showed that increased eNOS expression level is associated with post-diabetes depression. Therefore, it can be a good treatment goal for type 2 diabetic patients with depression. VL - 5 IS - 2 ER -
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