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Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism

Received: 22 August 2019     Accepted: 11 September 2019     Published: 23 October 2019
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Abstract

A κ-carrageenan degrading bacterium was isolated from decaying red seaweeds. Based on biochemical characterization and 16S rRNA sequence the isolate was identified as Alteromonas macleodii KS62. To the best of our knowledge, this is the first study reporting κ-carrageenan degrading bacterium of species Alteromonas macleodii. κ-carrageenase enzyme production of 1.6U/ml (12.5U/mg protein) was obtained when Alteromonas macleodii KS62 was grown in a medium containing κ-carrageenan as carbon source. The κ-carrageenase production further increased to 2.9U/ml (14.1U/mg protein) when Alteromonas macleodii KS62 was grown in a medium supplemented with Kappaphycus red seaweed powder. This the first report on κ-carrageenase production using red seaweed biomass as substrate. Efficient κ-carrageenase production establishes the potential of red seaweed biomass to serve as a low cost substrate for economic production of κ-carrageenase enzymes. Enzymatic hydrolysis of κ-carrageenan was studied. κ-carrageenan tetrasaccharide and hexasaccharide were found to be the major end products of enzymatic hydrolysis.

Published in International Journal of Microbiology and Biotechnology (Volume 4, Issue 4)
DOI 10.11648/j.ijmb.20190404.13
Page(s) 128-132
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Alteromonas macleodii, Κ-carrageenan, Κ-carrageenase, Enzyme, Red Seaweeds, Kappaphycus

References
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[2] Wang W., Zhang P., Yu G. L., et al (2012) Preparation and anti-influenza A virus activity of κ-carrageenan oligosaccharide and its sulphated derivatives. Food Chem 133: 880–888.
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[7] Khambhaty Y., Mody K. and Jha B. (2007) Purification and characterization of κ-carrageenase from a novel γ-proteobacterium, Pseudomonas elongata (MTCC 5261) syn. Microbulbifer elongatus comb. nov. Biotechnol Bioprocess Eng 12: 668–675.
[8] Sun F., Ma Y., Wang Y. and Liu Q. (2010) Purification and characterization of novel κ-carrageenase from marine Tamlana sp. HC4. Chinese J Oceanol Limnol 28: 1139–1145.
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[12] Youssef S. A., Beltagy E. A., El-Shenawy M. A. and El-Assar, S. A. (2012) Production of k-carrageenase by Cellulosimicrobium cellulans isolated from Egyptian Mediterranean coast. African J Microbiol Res 6: 6618–6628.
[13] Yao Z., Wang F., Gao Z., et al (2013) Characterization of a κ-carrageenase from marine Cellulophaga lytica strain N5-2 and analysis of its degradation products. Int J Mol Sci 14: 24592–602.
[14] Sun Y., Liu Y., Jiang K., et al (2014) Electrospray ionization mass spectrometric analysis of κ-carrageenan oligosaccharides obtained by degradation with κ-carrageenase from Pedobacter hainanensis. J Agric Food Chem 62: 2398−2405.
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    Prajakta Naval, Chandra Sainathan. (2019). Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism. International Journal of Microbiology and Biotechnology, 4(4), 128-132. https://doi.org/10.11648/j.ijmb.20190404.13

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    ACS Style

    Prajakta Naval; Chandra Sainathan. Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism. Int. J. Microbiol. Biotechnol. 2019, 4(4), 128-132. doi: 10.11648/j.ijmb.20190404.13

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    AMA Style

    Prajakta Naval, Chandra Sainathan. Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism. Int J Microbiol Biotechnol. 2019;4(4):128-132. doi: 10.11648/j.ijmb.20190404.13

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  • @article{10.11648/j.ijmb.20190404.13,
      author = {Prajakta Naval and Chandra Sainathan},
      title = {Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {4},
      number = {4},
      pages = {128-132},
      doi = {10.11648/j.ijmb.20190404.13},
      url = {https://doi.org/10.11648/j.ijmb.20190404.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190404.13},
      abstract = {A κ-carrageenan degrading bacterium was isolated from decaying red seaweeds. Based on biochemical characterization and 16S rRNA sequence the isolate was identified as Alteromonas macleodii KS62. To the best of our knowledge, this is the first study reporting κ-carrageenan degrading bacterium of species Alteromonas macleodii. κ-carrageenase enzyme production of 1.6U/ml (12.5U/mg protein) was obtained when Alteromonas macleodii KS62 was grown in a medium containing κ-carrageenan as carbon source. The κ-carrageenase production further increased to 2.9U/ml (14.1U/mg protein) when Alteromonas macleodii KS62 was grown in a medium supplemented with Kappaphycus red seaweed powder. This the first report on κ-carrageenase production using red seaweed biomass as substrate. Efficient κ-carrageenase production establishes the potential of red seaweed biomass to serve as a low cost substrate for economic production of κ-carrageenase enzymes. Enzymatic hydrolysis of κ-carrageenan was studied. κ-carrageenan tetrasaccharide and hexasaccharide were found to be the major end products of enzymatic hydrolysis.},
     year = {2019}
    }
    

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    T1  - Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism
    AU  - Prajakta Naval
    AU  - Chandra Sainathan
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    DO  - 10.11648/j.ijmb.20190404.13
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
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    EP  - 132
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20190404.13
    AB  - A κ-carrageenan degrading bacterium was isolated from decaying red seaweeds. Based on biochemical characterization and 16S rRNA sequence the isolate was identified as Alteromonas macleodii KS62. To the best of our knowledge, this is the first study reporting κ-carrageenan degrading bacterium of species Alteromonas macleodii. κ-carrageenase enzyme production of 1.6U/ml (12.5U/mg protein) was obtained when Alteromonas macleodii KS62 was grown in a medium containing κ-carrageenan as carbon source. The κ-carrageenase production further increased to 2.9U/ml (14.1U/mg protein) when Alteromonas macleodii KS62 was grown in a medium supplemented with Kappaphycus red seaweed powder. This the first report on κ-carrageenase production using red seaweed biomass as substrate. Efficient κ-carrageenase production establishes the potential of red seaweed biomass to serve as a low cost substrate for economic production of κ-carrageenase enzymes. Enzymatic hydrolysis of κ-carrageenan was studied. κ-carrageenan tetrasaccharide and hexasaccharide were found to be the major end products of enzymatic hydrolysis.
    VL  - 4
    IS  - 4
    ER  - 

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Author Information
  • Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India

  • Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India

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