Regulating Oncogenic lncRNA VPS9D1-AS1 with Cholesterol-Modified siRNA for Esophageal Squamous Cell Carcinoma Therapy
Yuan Mu,
Xinyue Wang,
Wenqiu Cai,
Xiaojing Liu,
Qian Zou,
Tiantian Li,
Wei Du,
Hongyan Zhan,
Sheng Tan,
Hanmei Xu,
Mengwei Li
Issue:
Volume 11, Issue 3, September 2023
Pages:
23-31
Received:
7 September 2023
Accepted:
27 September 2023
Published:
14 October 2023
Abstract: Esophageal carcinoma is the tenth most common cancer in the world. long non-coding RNA (lncRNA) is a type of RNA molecule that has no or limited coding ability. Many studies have found that it plays an important role in the occurrence and development of tumors. This article aims to investigate the expression and clinical significance of lncRNA VPS9D1-AS1 in patients with esophageal squamous cell carcinoma (ESCC). Additionally, it aims to examine the effects of targeted silencing of VPS9D1-AS1 on the growth and migration of ESCC cells in vivo and in vitro, and to explore the mechanism of action. This study compared the expression difference of lncRNA VPS9D1-AS1 in ESCC tissues and normal tissues through TCGA and UCSC databases, and analyzed the correlation between VPS9D1-AS1 expression and the prognosis of ESCC patients; clinical samples from ESCC patients were collected and qRT-PCR (Fluorescent quantitative polymerase chain reaction) was used to detect the relative expression of VPS9D1-AS1 and analyze the correlation between the expression of VPS9D1-AS1 and the onset and development of ESCC. Specific small interfering RNA was used to knock down the expression of VPS9D1-AS1 in ESCC cells, and the effect of VPS9D1-AS1 on the proliferation and migration of esophageal squamous cell carcinoma cells was detected by CCK-8 and Transwell assays. A subcutaneous transplantation tumor model of ESCC cells was established, and cholesterol-modified si-VPS9D1-AS1 was injected around the tumor to detect the impact of VPS9D1-AS1 on ESCC tumor growth in vivo. Western blot was used to detect the expression of apoptosis-related proteins in the tumor tissues of mice in each group. Finally, it was found that lncRNA VPS9D1-AS1 was highly expressed in ESCC tissues and significantly correlated with the prognosis of ESCC patients. Knocking down VPS9D1-AS1 regulates the proliferation, migration, and apoptosis of ESCC cells, and inhibits the growth of transplanted tumors in vivo, indicating it is a potential diagnostic and therapeutic target for esophageal squamous cell carcinoma.
Abstract: Esophageal carcinoma is the tenth most common cancer in the world. long non-coding RNA (lncRNA) is a type of RNA molecule that has no or limited coding ability. Many studies have found that it plays an important role in the occurrence and development of tumors. This article aims to investigate the expression and clinical significance of lncRNA VPS9...
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Research Article
Exploring Barriers and Perceptions of Cervical Cancer Screening Among Reproductive Age Rural Women in Sadi Chanka District, Oromia, West Ethiopia: A Qualitative Study
Ararso Hordofa Guye,
Dame Banti Shambi,
Tadesse Nigussie,
Elsabeth Lagesse,
Negasa Shuma Dureso,
Efa Bayissa Kanea,
Kasim Hansa
Issue:
Volume 11, Issue 3, September 2023
Pages:
32-37
Received:
8 September 2023
Accepted:
28 September 2023
Published:
28 October 2023
Abstract: Background: Cervical cancer is the leading fourth most common disease affecting the reproductive organs of the women and challenges faced by women in their life. Cervical cancer screening is one of the global public health intervention used on a population at risk, or target population to diagnose a disease. Effective cervical cancer screening programs reduce morbidity and mortality due to this cancer. The study aimed to explore barriers and perceptions of cervical cancer screening among reproductive-age rural women in Sadi Chanka district, Oromia, West Ethiopia. Methods: A qualitative study was conducted in Sadi Chanka District of West Ethiopia through in-depth interviews with 28 study participants from November 1 to December 20, 2022. Data were collected by using semi-structured questionnaires and captured using an audio tape recorder and field note-taking. A homogeneous purposive sampling technique was used to select representative study participants. Participants' interviews were transcribed and translated according to the participants verbatim from the local language (Afan-Oromo) to the English language by language experts in both languages. The transcribed data were entered into Microsoft Word and analyzed using thematic analysis and presented in narrative forms using the respondent's verbatim as an illustration. Results: A total of twenty-eight study participants were involved in this study. Seven thematic areas were emerged from interviews of the respondents through thematic analysis of the data: The identified barriers and perceptions of the respondents towards cervical screening were categorized into the following thematic areas; Misinformation and awareness-related factors, the signs and symptoms of cervical cancer, gender preference for cervical screening services, side effects of the procedures, availability and affordability of the services, and prevention and treatment outcome of the disease were the key identified barriers and perceptions of cervical cancer screening. Conclusion: The finding explored and revealed that misinformation, lack of awareness about cervical cancer screening services, lack of cervical screening services at all health facilities, the risk factors of cervical cancer, signs and symptoms of cervical cancer, gender preference for screening services, side effects of procedures, availability and affordability of the cervical cancer screening services, and prevention and treatment outcome of the disease were the key barriers and perceptions of cervical screening among rural reproductive age women. Therefore, the Sadi Chanka district health office should work on cervical cancer screening services to improve rural women's barriers and perceptions of cervical cancer service screening, efforts should be focused on enhancing awareness and related factors.
Abstract: Background: Cervical cancer is the leading fourth most common disease affecting the reproductive organs of the women and challenges faced by women in their life. Cervical cancer screening is one of the global public health intervention used on a population at risk, or target population to diagnose a disease. Effective cervical cancer screening prog...
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