Research Article
Investigation of the Interaction Between Ovalbumin and Gallic Acid by Spectroscopic Method
Su-Chol Rim,
Hang-Ryol Maeng*
,
Song-Jin Pak,
Yong-Su Pak,
Hyok-Won Kim
Issue:
Volume 11, Issue 1, March 2025
Pages:
1-7
Received:
10 November 2024
Accepted:
12 December 2024
Published:
21 January 2025
DOI:
10.11648/j.jddmc.20251101.11
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Views:
Abstract: Gallic acid, one of the polyphenolic compounds, has known to have many pharmaceutical and biological properties such as anti-oxidant, anti-inflammatory, anti-virus and anti-tumor activities. The interaction study of gallic acid and protein is very important in drug design, development of drug delivery system as well as immunology research. Among various techniques available for interaction study between polyphenols and small molecule, spectroscopic method is mainly used to assess protein-polyphenol complex due to its simplicity, rapidity and sensitivity. In this study, the interaction between gallic acid and ovalbumin was investigated by fluorescence quenching, synchronous and 3D (three-dimensional) fluorescence spectra. The possibility of quenching of ovalbumin by gallic acid was studied by Stern-Volmer equation. The quenching mechanism, binding constant, the number of binding sites were also investigated. The binding mode was determined using the Van’t Hoff and Gibbs-Helmholtz equation. The average distance between gallic acid and ovalbumin was calculated by Förster’s theory. The results showed that gallic acid could quench ovalbumin through static quenching mechanism. In the binding process, electrostatic forces were mainly involved. In addition, synchronous and 3D fluorescence spectroscopic analysis revealed that the microenvironments of tryptophan and tyrosine residues in ovalbumin were changed during the complex formation. The result of present study provides the information of binding mechanism of gallic acid with ovalbumin.
Abstract: Gallic acid, one of the polyphenolic compounds, has known to have many pharmaceutical and biological properties such as anti-oxidant, anti-inflammatory, anti-virus and anti-tumor activities. The interaction study of gallic acid and protein is very important in drug design, development of drug delivery system as well as immunology research. Among va...
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