Research Article
NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon
Marie-Claire Vernyuy Fonyuy,
Calvino Fomboh Tah,
Akindeh Mbuh Nji,
Isabelle Mboutchuin Kamdem Winnie,
Jean Paul Kengne Chedjou,
Magellan Guewo Fokeng,
Aristid Herve Ekollo Mbange,
Wilfried Olivier Ngandjeu Tchamdjeu,
Lesley Ngum Ngum,
Peter Thelma Ngwa Niba,
Rodrigue Essomba Foe,
Cedric Hermann Dongmo,
Carine Nguefeu Nkenfou-Tchinda,
Rhoda Bongshe Laban,
Yusinyu Eugenie Mumah,
Eugene Sobngwi,
Jean Claude Mbanya,
Wilfred Fon Mbacham*
Issue:
Volume 8, Issue 3, September 2023
Pages:
37-44
Received:
27 October 2023
Accepted:
14 November 2023
Published:
30 November 2023
Abstract: Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Polymorphisms (rs10754558 and rs4612666) in the NLRP3 gene could be associated with T2DM and malaria comorbidity is relatively new. This study aimed at determining the association between NLRP3 rs10754558 and rs4612666 Single Nucleotide Polymorphisms with susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in Yaoundé, Cameroon. Methods: A case-control study was performed on 100 conveniently collected blood samples, spotted on Whartman N° 3 filter paper from which DNA was extracted by the chelex-100 boiling method. Nested-PCR was used to confirm the presence of malaria and speciate Plasmodium spp. Genotyping of the NLRP3 gene SNPs was performed using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi-square test (X2) was used to establish associations. A P-value of <0.05 was considered significant. Results: The mean age of the study population was 55±12.38 years. Eighty-eight (88) participants were diagnosed with T2DM, whereof 7 (7.95%) were ascertained by nested-PCR to harbour malaria; P. falciparum being the dominant circulating species. The most predominant genotype and allele for rs10754558 and rs4612666, was the heterozygous genotype GC and wildtype allele G (52.00%, 69.00%), and the homozygous mutant genotype CC and mutant allele C (63.00%, 76.50%) respectively. No statistical significance was found between the comorbid group and diabetes positive /malaria negative (D+M-) control group for the rs10754558 and rs4612666 SNPs. Statistical significance was found between the comorbid group and the diabetes negative/ malaria positive (D-M+) control group for the rs4612666 SNP. Individuals possessing the CC genotypes were 8 times more susceptible to diabetes and malaria comorbidity (OR=8.000, P=0.043), whereas individuals possessing the TC genotype were less susceptible (OR=0.079, P=0.030). Conclusion: An association was found between the NLRP3 rs4612666 SNP and susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in our study.
Abstract: Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Po...
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