Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status

Cuicui Wang; Zhaoxin Mu; Yunxia Chen; Jianfeng Liu; Jinqun Ma; Chunyan Liu; Zhenjiang Hou

doi:doi:10.11648/j.sjph.20210901.12

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Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status

Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, Chunyan Liu, Zhenjiang Hou

Published in Science Journal of Public Health (Volume 9, Issue 1)

Received: 16 December 2020 Accepted: Published: 18 January 2021

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Abstract

Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (P<0.05), while Tregs cell proportion, serum TGF-β and Foxp3 mRNA level were all lower than those in the control group (P<0.05). Conclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.

Published in	Science Journal of Public Health (Volume 9, Issue 1)
DOI	10.11648/j.sjph.20210901.12
Page(s)	12-22
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Th17/Treg Cells, Cytokines, Transcription Factors, Iodine Nutritional Status, Hashimoto's Thyroiditis, Thyroid Autoantibodies

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APA Style

Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, et al. (2021). Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Science Journal of Public Health, 9(1), 12-22. https://doi.org/10.11648/j.sjph.20210901.12

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ACS Style

Cuicui Wang; Zhaoxin Mu; Yunxia Chen; Jianfeng Liu; Jinqun Ma, et al. Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Sci. J. Public Health 2021, 9(1), 12-22. doi: 10.11648/j.sjph.20210901.12

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AMA Style

Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, et al. Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Sci J Public Health. 2021;9(1):12-22. doi: 10.11648/j.sjph.20210901.12

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@article{10.11648/j.sjph.20210901.12,
  author = {Cuicui Wang and Zhaoxin Mu and Yunxia Chen and Jianfeng Liu and Jinqun Ma and Chunyan Liu and Zhenjiang Hou},
  title = {Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {1},
  pages = {12-22},
  doi = {10.11648/j.sjph.20210901.12},
  url = {https://doi.org/10.11648/j.sjph.20210901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210901.12},
  abstract = {Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (PPConclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.},
 year = {2021}
}

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TY  - JOUR
T1  - Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status
AU  - Cuicui Wang
AU  - Zhaoxin Mu
AU  - Yunxia Chen
AU  - Jianfeng Liu
AU  - Jinqun Ma
AU  - Chunyan Liu
AU  - Zhenjiang Hou
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210901.12
DO  - 10.11648/j.sjph.20210901.12
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 12
EP  - 22
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210901.12
AB  - Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (PPConclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.
VL  - 9
IS  - 1
ER  -

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Author Information

Cuicui Wang

Department of Medical Technology, Cangzhou Medical College, Cangzhou, China
Zhaoxin Mu

Cangzhou Thyroid Disease Engineering Research Center, Cangzhou Medical College, Cangzhou, China
Yunxia Chen

Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China
Jianfeng Liu

Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China
Jinqun Ma

Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China
Chunyan Liu

Cangzhou Thyroid Disease Engineering Research Center, Cangzhou Medical College, Cangzhou, China
Zhenjiang Hou

Cangzhou Thyroid Disease Engineering Research Center, Cangzhou Medical College, Cangzhou, China

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Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, et al. (2021). Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Science Journal of Public Health, 9(1), 12-22. https://doi.org/10.11648/j.sjph.20210901.12

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ACS Style

Cuicui Wang; Zhaoxin Mu; Yunxia Chen; Jianfeng Liu; Jinqun Ma, et al. Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Sci. J. Public Health 2021, 9(1), 12-22. doi: 10.11648/j.sjph.20210901.12

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AMA Style

Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, et al. Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Sci J Public Health. 2021;9(1):12-22. doi: 10.11648/j.sjph.20210901.12

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@article{10.11648/j.sjph.20210901.12,
  author = {Cuicui Wang and Zhaoxin Mu and Yunxia Chen and Jianfeng Liu and Jinqun Ma and Chunyan Liu and Zhenjiang Hou},
  title = {Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {1},
  pages = {12-22},
  doi = {10.11648/j.sjph.20210901.12},
  url = {https://doi.org/10.11648/j.sjph.20210901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210901.12},
  abstract = {Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (PPConclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.},
 year = {2021}
}

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TY  - JOUR
T1  - Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status
AU  - Cuicui Wang
AU  - Zhaoxin Mu
AU  - Yunxia Chen
AU  - Jianfeng Liu
AU  - Jinqun Ma
AU  - Chunyan Liu
AU  - Zhenjiang Hou
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210901.12
DO  - 10.11648/j.sjph.20210901.12
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 12
EP  - 22
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210901.12
AB  - Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (PPConclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.
VL  - 9
IS  - 1
ER  -

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