Fructo-oligosaccharides is a natural active substance with excellent physiological functions such as improving the gastrointestinal tract, regulating intestinal flora, etc. It is an important nutrient in infant dairy products. Therefore, it is extremely important to accurately detect fructo-oligosaccharides in infant dairy products. Nowadays, the main methods for detection of fructo-oligosaccharides at home and abroad include high performance liquid chromatography, ion exchange chromatography, mass spectrometry, etc. In this article, a method for the determination of fructo-oligosaccharides in modified milk and infant formula milk powder by high performance liquid chromatography (HPLC) was developed. Fructo-oligosaccharides was extracted by water. Protein was precipitated by acetonitrile. The method was performed on NH2 column, the detector was refractive index detector (RID) and the mobile phase was 70% acetonitrile. Fructo-oligosaccharides content of modified milk was in the range of 20-500mg/100g, the recovery was 80.2%-107.1%. The content of Fructo-oligosaccharides in infant formula milk powder was in the range of 100-500mg/100g, the recovery was 71.9%-95.3%. The quantitative detection limit was 20mg/100g. This method was easily to be operated with a high accuracy and the results indicated that it was suitable for the analysis of fructo-oligosaccharides in modified milk and infant formula milk powder.
| Published in | International Journal of Food Engineering and Technology (Volume 2, Issue 2) |
| DOI | 10.11648/j.ijfet.20180202.14 |
| Page(s) | 36-40 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
HPLC, Fructo-Oligosaccharides, Modified Milk, Infant Formula Milk Powder
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APA Style
Zhen Zhao, Guoyan Wen, Cuizhi Li, Peng Wan, Zhiyong Lv. (2019). HPLC Determination of Fructo-Oligosaccharides in Dairy Products. International Journal of Food Engineering and Technology, 2(2), 36-40. https://doi.org/10.11648/j.ijfet.20180202.14
ACS Style
Zhen Zhao; Guoyan Wen; Cuizhi Li; Peng Wan; Zhiyong Lv. HPLC Determination of Fructo-Oligosaccharides in Dairy Products. Int. J. Food Eng. Technol. 2019, 2(2), 36-40. doi: 10.11648/j.ijfet.20180202.14
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Download@article{10.11648/j.ijfet.20180202.14,
author = {Zhen Zhao and Guoyan Wen and Cuizhi Li and Peng Wan and Zhiyong Lv},
title = {HPLC Determination of Fructo-Oligosaccharides in Dairy Products},
journal = {International Journal of Food Engineering and Technology},
volume = {2},
number = {2},
pages = {36-40},
doi = {10.11648/j.ijfet.20180202.14},
url = {https://doi.org/10.11648/j.ijfet.20180202.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfet.20180202.14},
abstract = {Fructo-oligosaccharides is a natural active substance with excellent physiological functions such as improving the gastrointestinal tract, regulating intestinal flora, etc. It is an important nutrient in infant dairy products. Therefore, it is extremely important to accurately detect fructo-oligosaccharides in infant dairy products. Nowadays, the main methods for detection of fructo-oligosaccharides at home and abroad include high performance liquid chromatography, ion exchange chromatography, mass spectrometry, etc. In this article, a method for the determination of fructo-oligosaccharides in modified milk and infant formula milk powder by high performance liquid chromatography (HPLC) was developed. Fructo-oligosaccharides was extracted by water. Protein was precipitated by acetonitrile. The method was performed on NH2 column, the detector was refractive index detector (RID) and the mobile phase was 70% acetonitrile. Fructo-oligosaccharides content of modified milk was in the range of 20-500mg/100g, the recovery was 80.2%-107.1%. The content of Fructo-oligosaccharides in infant formula milk powder was in the range of 100-500mg/100g, the recovery was 71.9%-95.3%. The quantitative detection limit was 20mg/100g. This method was easily to be operated with a high accuracy and the results indicated that it was suitable for the analysis of fructo-oligosaccharides in modified milk and infant formula milk powder.},
year = {2019}
}
TY - JOUR T1 - HPLC Determination of Fructo-Oligosaccharides in Dairy Products AU - Zhen Zhao AU - Guoyan Wen AU - Cuizhi Li AU - Peng Wan AU - Zhiyong Lv Y1 - 2019/01/11 PY - 2019 N1 - https://doi.org/10.11648/j.ijfet.20180202.14 DO - 10.11648/j.ijfet.20180202.14 T2 - International Journal of Food Engineering and Technology JF - International Journal of Food Engineering and Technology JO - International Journal of Food Engineering and Technology SP - 36 EP - 40 PB - Science Publishing Group SN - 2640-1584 UR - https://doi.org/10.11648/j.ijfet.20180202.14 AB - Fructo-oligosaccharides is a natural active substance with excellent physiological functions such as improving the gastrointestinal tract, regulating intestinal flora, etc. It is an important nutrient in infant dairy products. Therefore, it is extremely important to accurately detect fructo-oligosaccharides in infant dairy products. Nowadays, the main methods for detection of fructo-oligosaccharides at home and abroad include high performance liquid chromatography, ion exchange chromatography, mass spectrometry, etc. In this article, a method for the determination of fructo-oligosaccharides in modified milk and infant formula milk powder by high performance liquid chromatography (HPLC) was developed. Fructo-oligosaccharides was extracted by water. Protein was precipitated by acetonitrile. The method was performed on NH2 column, the detector was refractive index detector (RID) and the mobile phase was 70% acetonitrile. Fructo-oligosaccharides content of modified milk was in the range of 20-500mg/100g, the recovery was 80.2%-107.1%. The content of Fructo-oligosaccharides in infant formula milk powder was in the range of 100-500mg/100g, the recovery was 71.9%-95.3%. The quantitative detection limit was 20mg/100g. This method was easily to be operated with a high accuracy and the results indicated that it was suitable for the analysis of fructo-oligosaccharides in modified milk and infant formula milk powder. VL - 2 IS - 2 ER -
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