Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia

Shibiru Eticha; Tesfa Bedassa

doi:doi:10.11648/j.ijbbmb.20200501.12

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Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia

Shibiru Eticha, Tesfa Bedassa

Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 5, Issue 1)

Received: 15 May 2020 Accepted: 1 June 2020 Published: 27 August 2020

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Abstract

In this research caffeine content in coffee sample from Abe Dongoro, Sasiga, Gida Ayana and Sibu Sire of Wollega administrative zone of Ethiopia were determined using High Performance Liquid Chromatography (HPLC) and UV-Vis Spectrophotometry methods. Caffeine in aqueous extract of coffee samples was extracted with dichloromethane prior to analysis by UV-Vis spectrophotometry method and dichloromethane was evaporated from the extract and the extract was dissolved in water (HPLC grade) to determine caffeine contents in coffee samples using HPLC method. The linearity of the HPLC and UV-Vis spectrophotometry methods were R² = 0.9999 and R² = 0.9997 respectively. HPLC and UV-Vis spectrophotometry methods were found to be accurate with recoveries of 97.5% and 117.4%, respectively. Limits of detection (LOD) obtained were 0.148 mg/L for HPLC method and 0.284 mg/L for UV-Vis spectrophotometric method. The caffeine contents in coffee samples obtained using UV-Vis spectrophotometry method was 3.42, 2.638, 2.207 and 2.986 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples respectively. Similarly, using HPLC method the caffeine contents in coffee samples obtained was 1.871, 1.601, 1.307, 1.83 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples. There is a significant difference between the caffeine contents in coffee samples obtained by the two methods.

Published in	International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 5, Issue 1)
DOI	10.11648/j.ijbbmb.20200501.12
Page(s)	8-17
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Coffee Samples, Caffeine, UV-Vis Spectrophotometry, High Performance Liquid Chromatography, Horro Guduru Wollega, East Wollega

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APA Style

Shibiru Eticha, Tesfa Bedassa. (2020). Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia. International Journal of Biochemistry, Biophysics & Molecular Biology, 5(1), 8-17. https://doi.org/10.11648/j.ijbbmb.20200501.12

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ACS Style

Shibiru Eticha; Tesfa Bedassa. Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia. Int. J. Biochem. Biophys. Mol. Biol. 2020, 5(1), 8-17. doi: 10.11648/j.ijbbmb.20200501.12

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AMA Style

Shibiru Eticha, Tesfa Bedassa. Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia. Int J Biochem Biophys Mol Biol. 2020;5(1):8-17. doi: 10.11648/j.ijbbmb.20200501.12

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@article{10.11648/j.ijbbmb.20200501.12,
  author = {Shibiru Eticha and Tesfa Bedassa},
  title = {Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {5},
  number = {1},
  pages = {8-17},
  doi = {10.11648/j.ijbbmb.20200501.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20200501.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20200501.12},
  abstract = {In this research caffeine content in coffee sample from Abe Dongoro, Sasiga, Gida Ayana and Sibu Sire of Wollega administrative zone of Ethiopia were determined using High Performance Liquid Chromatography (HPLC) and UV-Vis Spectrophotometry methods. Caffeine in aqueous extract of coffee samples was extracted with dichloromethane prior to analysis by UV-Vis spectrophotometry method and dichloromethane was evaporated from the extract and the extract was dissolved in water (HPLC grade) to determine caffeine contents in coffee samples using HPLC method. The linearity of the HPLC and UV-Vis spectrophotometry methods were R2 = 0.9999 and R2 = 0.9997 respectively. HPLC and UV-Vis spectrophotometry methods were found to be accurate with recoveries of 97.5% and 117.4%, respectively. Limits of detection (LOD) obtained were 0.148 mg/L for HPLC method and 0.284 mg/L for UV-Vis spectrophotometric method. The caffeine contents in coffee samples obtained using UV-Vis spectrophotometry method was 3.42, 2.638, 2.207 and 2.986 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples respectively. Similarly, using HPLC method the caffeine contents in coffee samples obtained was 1.871, 1.601, 1.307, 1.83 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples. There is a significant difference between the caffeine contents in coffee samples obtained by the two methods.},
 year = {2020}
}

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TY  - JOUR
T1  - Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia
AU  - Shibiru Eticha
AU  - Tesfa Bedassa
Y1  - 2020/08/27
PY  - 2020
N1  - https://doi.org/10.11648/j.ijbbmb.20200501.12
DO  - 10.11648/j.ijbbmb.20200501.12
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 8
EP  - 17
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20200501.12
AB  - In this research caffeine content in coffee sample from Abe Dongoro, Sasiga, Gida Ayana and Sibu Sire of Wollega administrative zone of Ethiopia were determined using High Performance Liquid Chromatography (HPLC) and UV-Vis Spectrophotometry methods. Caffeine in aqueous extract of coffee samples was extracted with dichloromethane prior to analysis by UV-Vis spectrophotometry method and dichloromethane was evaporated from the extract and the extract was dissolved in water (HPLC grade) to determine caffeine contents in coffee samples using HPLC method. The linearity of the HPLC and UV-Vis spectrophotometry methods were R2 = 0.9999 and R2 = 0.9997 respectively. HPLC and UV-Vis spectrophotometry methods were found to be accurate with recoveries of 97.5% and 117.4%, respectively. Limits of detection (LOD) obtained were 0.148 mg/L for HPLC method and 0.284 mg/L for UV-Vis spectrophotometric method. The caffeine contents in coffee samples obtained using UV-Vis spectrophotometry method was 3.42, 2.638, 2.207 and 2.986 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples respectively. Similarly, using HPLC method the caffeine contents in coffee samples obtained was 1.871, 1.601, 1.307, 1.83 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples. There is a significant difference between the caffeine contents in coffee samples obtained by the two methods.
VL  - 5
IS  - 1
ER  -

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Author Information

Shibiru Eticha

Department of Chemistry, Wollega University, Nekemte, Ethiopia
Tesfa Bedassa

Department of Chemistry, Wollega University, Nekemte, Ethiopia

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APA Style

Shibiru Eticha, Tesfa Bedassa. (2020). Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia. International Journal of Biochemistry, Biophysics & Molecular Biology, 5(1), 8-17. https://doi.org/10.11648/j.ijbbmb.20200501.12

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ACS Style

Shibiru Eticha; Tesfa Bedassa. Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia. Int. J. Biochem. Biophys. Mol. Biol. 2020, 5(1), 8-17. doi: 10.11648/j.ijbbmb.20200501.12

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AMA Style

Shibiru Eticha, Tesfa Bedassa. Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia. Int J Biochem Biophys Mol Biol. 2020;5(1):8-17. doi: 10.11648/j.ijbbmb.20200501.12

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@article{10.11648/j.ijbbmb.20200501.12,
  author = {Shibiru Eticha and Tesfa Bedassa},
  title = {Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {5},
  number = {1},
  pages = {8-17},
  doi = {10.11648/j.ijbbmb.20200501.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20200501.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20200501.12},
  abstract = {In this research caffeine content in coffee sample from Abe Dongoro, Sasiga, Gida Ayana and Sibu Sire of Wollega administrative zone of Ethiopia were determined using High Performance Liquid Chromatography (HPLC) and UV-Vis Spectrophotometry methods. Caffeine in aqueous extract of coffee samples was extracted with dichloromethane prior to analysis by UV-Vis spectrophotometry method and dichloromethane was evaporated from the extract and the extract was dissolved in water (HPLC grade) to determine caffeine contents in coffee samples using HPLC method. The linearity of the HPLC and UV-Vis spectrophotometry methods were R2 = 0.9999 and R2 = 0.9997 respectively. HPLC and UV-Vis spectrophotometry methods were found to be accurate with recoveries of 97.5% and 117.4%, respectively. Limits of detection (LOD) obtained were 0.148 mg/L for HPLC method and 0.284 mg/L for UV-Vis spectrophotometric method. The caffeine contents in coffee samples obtained using UV-Vis spectrophotometry method was 3.42, 2.638, 2.207 and 2.986 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples respectively. Similarly, using HPLC method the caffeine contents in coffee samples obtained was 1.871, 1.601, 1.307, 1.83 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples. There is a significant difference between the caffeine contents in coffee samples obtained by the two methods.},
 year = {2020}
}

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TY  - JOUR
T1  - Determination of Caffeine in Coffee Samples by High Performance Liquid Chromatography and Ultra Violet - Visible Spectrophotometry Methods from Wollega, Ethiopia
AU  - Shibiru Eticha
AU  - Tesfa Bedassa
Y1  - 2020/08/27
PY  - 2020
N1  - https://doi.org/10.11648/j.ijbbmb.20200501.12
DO  - 10.11648/j.ijbbmb.20200501.12
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 8
EP  - 17
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20200501.12
AB  - In this research caffeine content in coffee sample from Abe Dongoro, Sasiga, Gida Ayana and Sibu Sire of Wollega administrative zone of Ethiopia were determined using High Performance Liquid Chromatography (HPLC) and UV-Vis Spectrophotometry methods. Caffeine in aqueous extract of coffee samples was extracted with dichloromethane prior to analysis by UV-Vis spectrophotometry method and dichloromethane was evaporated from the extract and the extract was dissolved in water (HPLC grade) to determine caffeine contents in coffee samples using HPLC method. The linearity of the HPLC and UV-Vis spectrophotometry methods were R2 = 0.9999 and R2 = 0.9997 respectively. HPLC and UV-Vis spectrophotometry methods were found to be accurate with recoveries of 97.5% and 117.4%, respectively. Limits of detection (LOD) obtained were 0.148 mg/L for HPLC method and 0.284 mg/L for UV-Vis spectrophotometric method. The caffeine contents in coffee samples obtained using UV-Vis spectrophotometry method was 3.42, 2.638, 2.207 and 2.986 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples respectively. Similarly, using HPLC method the caffeine contents in coffee samples obtained was 1.871, 1.601, 1.307, 1.83 mg/L for Abe Dongoro, Gida Ayana, Sasiga and Sibu Sire coffee samples. There is a significant difference between the caffeine contents in coffee samples obtained by the two methods.
VL  - 5
IS  - 1
ER  -

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