Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance

Yulianela Mengana Torres; Manuel Arsenio Lores Guevara; Juan Carlos García Naranjo; Beatriz Taimy Ricardo Ferro; Lidia Clara Suárez Beyries; Inocente Clemente Rodríguez Reyes; María A. Marichal Feliu; Yomaidis Araujo Durán

doi:doi:10.11648/j.ijbbmb.20190402.12

Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance

Yulianela Mengana Torres, Manuel Arsenio Lores Guevara, Juan Carlos García Naranjo, Beatriz Taimy Ricardo Ferro, Lidia Clara Suárez Beyries, Inocente Clemente Rodríguez Reyes, María A. Marichal Feliu, Yomaidis Araujo Durán

Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 4, Issue 2)

Received: 11 December 2018 Accepted: 14 January 2019 Published: 10 January 2020

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Abstract

The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T₂). To experimentally determine the T₂ value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B₀ = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases.

Published in	International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 4, Issue 2)
DOI	10.11648/j.ijbbmb.20190402.12
Page(s)	25-30
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Proton Magnetic Resonance, Dynamic Blood Plasma Viscosity and Hemoglobin

References

[1]	Manuel A. Lores Guevara, Yulianela Mengana Torres, Juan C. García Naranjo, Norberto Rodríguez Suárez, Lidia C. Suáres Beyries, María A. Marichal Feliu, Teresa Simón Boada, Inocente C. Rodríguez Reyes, Jan Phillipé. Plasma Dynamic Viscosity Determined by NMR. Applied Magnetic Resonance 2018. DOI 10.1007/s00723-018-1026.
[2]	Mette Harreby, et all. Viscosity of Plasma in patients with rheumatoid arthritis. Annals of the rheumatic DiseasesAugust, 1987, 46 (8) 601-604. ISSN: 0003-4967.
[3]	Ajayi OI, Famodu AA, Oviasu E. Fibrinogen concentration. A marker of cardiovascular disorders in Nigerians. Turk J. Hematol; 2007, 24 (1), 18-22.
[4]	Rosencranz, R. and Steven A. Clinical Laboratory Measurement of Serum. Plasma and Blood Viscosity. Clin. Pathol; 2006, 125 (1), 78-86. ISSN: 0974-7532.
[5]	Recommendation for a selected method for the measurement of plasma viscosity. Clin. Pathol. 1984, 37 (10), 1147-1152.
[6]	A. Shames, Y. Rozenfeld, D. Keinius, Apparatus and method for non-invasive measurement of blood parameters, Patent application number: 2015/0018638A1, Publication date: 15 de Enero del 2015.
[7]	P.S. Wellman, M. Blanco, W. Peine, Portable device for nmr based analysis of rheological changes in liquid samples, Patent application number: US 2015/0130463A1, Publication date: may15, 2015.
[8]	Lee Josephson, Rui Hong, Michael J. Cima, Ralph Weissleder, Magnetic resonance-based viscometers and methods, Patent application number: WO 2009/026164A1, Publication date: 26 de Febrero de 2009.
[9]	Carlos Cabal Mirabal, Adolfo Fernández García, Manuel Lores Guevara, Evelio González, Leonardo Oramas Díaz. Kinetics studies of complex biomedical process by Magnetic Resonance. Cuban experiences. Applied Magnetic Resonance. https://doi.org/10.1007/s00723-018-0985-2
[10]	R. S. Menon and P. S. Allen. Solventprotonrelaxation of aqueoussolutions of theserumproteinsα2-macroglobulin, fibrinogen and albumin. Biophysical Journal. 1990, 57 (3), 389-396. doi: 10.1016/S0006-3495(90)82555-8.
[11]	M. Lores and C. Cabal. Proton Magnetic Relaxation Process during the Polymerization of Hemoglobin S. Appl. Magn.Reson. 2005, 28 (1), 79-84.
[12]	http://scielo.isciii.es/pdf/fh/v39n3/02articuloespecial01.pdf, revisado en marzo 2018.
[13]	Sánchez Hernández, B. E. Adolfo López Mateos, Viscosímetro de Bola, Tesis de Licenciatura de Química. Instituto Politécnico Nacional, México. D. F. 2008.
[14]	Galego Fernández, N. Manual de prácticas de laboratorio de Química Física de las Superficies y Polímeros. Editorial Pueblo y Educación, La Habana. página 48. 1986.
[15]	Lores Guevara, M. A. Estudios preliminares sobre el empleo de los radicales, en seguimiento de la Polimerización de la HbS. Tesis en opción del título de Ingeniero en Física Nuclear. Instituto Superior de Ciencias y tecnología Nuclear. 1993.
[16]	Guerra Bustillo C. W., Menéndez Acuña E., Barrera Morera R. y Egaña Morales E. Estadística. Editorial Pueblo y Educación. 1989.
[17]	www.quimica.urv.es/quimio/general/callin.pdf, revisado mayo 2018.
[18]	Lores Guevara, M. A. 2005. Estudio de los procesos de interacción Magnética y la movilidad molecular durante la polimerización de la HbS con métodos de Resonancia Magnética. Tesis presentada en opción al título de Doctor en Ciencias Físicas. Universidad de Oriente. Cuba.

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Yulianela Mengana Torres, Manuel Arsenio Lores Guevara, Juan Carlos García Naranjo, Beatriz Taimy Ricardo Ferro, Lidia Clara Suárez Beyries, et al. (2020). Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. International Journal of Biochemistry, Biophysics & Molecular Biology, 4(2), 25-30. https://doi.org/10.11648/j.ijbbmb.20190402.12

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ACS Style

Yulianela Mengana Torres; Manuel Arsenio Lores Guevara; Juan Carlos García Naranjo; Beatriz Taimy Ricardo Ferro; Lidia Clara Suárez Beyries, et al. Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. Int. J. Biochem. Biophys. Mol. Biol. 2020, 4(2), 25-30. doi: 10.11648/j.ijbbmb.20190402.12

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AMA Style

Yulianela Mengana Torres, Manuel Arsenio Lores Guevara, Juan Carlos García Naranjo, Beatriz Taimy Ricardo Ferro, Lidia Clara Suárez Beyries, et al. Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. Int J Biochem Biophys Mol Biol. 2020;4(2):25-30. doi: 10.11648/j.ijbbmb.20190402.12

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@article{10.11648/j.ijbbmb.20190402.12,
  author = {Yulianela Mengana Torres and Manuel Arsenio Lores Guevara and Juan Carlos García Naranjo and Beatriz Taimy Ricardo Ferro and Lidia Clara Suárez Beyries and Inocente Clemente Rodríguez Reyes and María A. Marichal Feliu and Yomaidis Araujo Durán},
  title = {Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {4},
  number = {2},
  pages = {25-30},
  doi = {10.11648/j.ijbbmb.20190402.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20190402.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20190402.12},
  abstract = {The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T2). To experimentally determine the T2 value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B0 = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases.},
 year = {2020}
}

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TY  - JOUR
T1  - Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance
AU  - Yulianela Mengana Torres
AU  - Manuel Arsenio Lores Guevara
AU  - Juan Carlos García Naranjo
AU  - Beatriz Taimy Ricardo Ferro
AU  - Lidia Clara Suárez Beyries
AU  - Inocente Clemente Rodríguez Reyes
AU  - María A. Marichal Feliu
AU  - Yomaidis Araujo Durán
Y1  - 2020/01/10
PY  - 2020
N1  - https://doi.org/10.11648/j.ijbbmb.20190402.12
DO  - 10.11648/j.ijbbmb.20190402.12
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 25
EP  - 30
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20190402.12
AB  - The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T2). To experimentally determine the T2 value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B0 = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases.
VL  - 4
IS  - 2
ER  -

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Author Information

Yulianela Mengana Torres

Centro de Biofísica Médica, Universidad de Oriente, Patricio Lumumba S/N. Santiago de Cuba. Cuba
Manuel Arsenio Lores Guevara

Centro de Biofísica Médica, Universidad de Oriente, Patricio Lumumba S/N. Santiago de Cuba. Cuba
Juan Carlos García Naranjo

Centro de Biofísica Médica, Universidad de Oriente, Patricio Lumumba S/N. Santiago de Cuba. Cuba
Beatriz Taimy Ricardo Ferro

Centro de Biofísica Médica, Universidad de Oriente, Patricio Lumumba S/N. Santiago de Cuba. Cuba
Lidia Clara Suárez Beyries

Hospital General “Dr. Juan Bruno Zayas Alfonso”, Carretera del Caney S/N, Santiago de Cuba. Cuba
Inocente Clemente Rodríguez Reyes

Hospital General “Dr. Juan Bruno Zayas Alfonso”, Carretera del Caney S/N, Santiago de Cuba. Cuba
María A. Marichal Feliu

Hospital General “Dr. Juan Bruno Zayas Alfonso”, Carretera del Caney S/N, Santiago de Cuba. Cuba
Yomaidis Araujo Durán

Hospital General “Dr. Juan Bruno Zayas Alfonso”, Carretera del Caney S/N, Santiago de Cuba. Cuba

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APA Style

Yulianela Mengana Torres, Manuel Arsenio Lores Guevara, Juan Carlos García Naranjo, Beatriz Taimy Ricardo Ferro, Lidia Clara Suárez Beyries, et al. (2020). Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. International Journal of Biochemistry, Biophysics & Molecular Biology, 4(2), 25-30. https://doi.org/10.11648/j.ijbbmb.20190402.12

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ACS Style

Yulianela Mengana Torres; Manuel Arsenio Lores Guevara; Juan Carlos García Naranjo; Beatriz Taimy Ricardo Ferro; Lidia Clara Suárez Beyries, et al. Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. Int. J. Biochem. Biophys. Mol. Biol. 2020, 4(2), 25-30. doi: 10.11648/j.ijbbmb.20190402.12

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AMA Style

Yulianela Mengana Torres, Manuel Arsenio Lores Guevara, Juan Carlos García Naranjo, Beatriz Taimy Ricardo Ferro, Lidia Clara Suárez Beyries, et al. Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance. Int J Biochem Biophys Mol Biol. 2020;4(2):25-30. doi: 10.11648/j.ijbbmb.20190402.12

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@article{10.11648/j.ijbbmb.20190402.12,
  author = {Yulianela Mengana Torres and Manuel Arsenio Lores Guevara and Juan Carlos García Naranjo and Beatriz Taimy Ricardo Ferro and Lidia Clara Suárez Beyries and Inocente Clemente Rodríguez Reyes and María A. Marichal Feliu and Yomaidis Araujo Durán},
  title = {Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {4},
  number = {2},
  pages = {25-30},
  doi = {10.11648/j.ijbbmb.20190402.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20190402.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20190402.12},
  abstract = {The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T2). To experimentally determine the T2 value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B0 = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Determination of Dynamic Viscosity in Samples of Blood Plasma and Hemoglobin Solution Using Nuclear Magnetic Resonance
AU  - Yulianela Mengana Torres
AU  - Manuel Arsenio Lores Guevara
AU  - Juan Carlos García Naranjo
AU  - Beatriz Taimy Ricardo Ferro
AU  - Lidia Clara Suárez Beyries
AU  - Inocente Clemente Rodríguez Reyes
AU  - María A. Marichal Feliu
AU  - Yomaidis Araujo Durán
Y1  - 2020/01/10
PY  - 2020
N1  - https://doi.org/10.11648/j.ijbbmb.20190402.12
DO  - 10.11648/j.ijbbmb.20190402.12
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 25
EP  - 30
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20190402.12
AB  - The viscosity evaluation in Plasma is extremely useful in the clinical evaluation of different diseases. A procedure is presented, based on Protonic Magnetic Resonance, for the evaluation of the dynamic viscosity in Blood Plasma and in hemoglobin solution from the determination of the transverse relaxation time (T2). To experimentally determine the T2 value, the impulse series Carr-Purcell-Meiboon-Gill was used in a MARAN DRX console (OXFORD INSTRUMENTS) and a homogeneous magnetic system (B0 = 0.095T). Values were obtained for the viscosity of the blood plasma and hemoglobin of 1.68 ± 0.12 mPas and 12.78 ± 3.55 mPas respectively, which agreed with the determined, in the same samples, using an Ostwald viscometer (1, 45 ± 0.06 mPas for the plasma and 12.82 ± 3.35 mPas for the dissolution of hemoglobin). The dynamic viscosity of the blood plasma was determined in 236 patients with Multiple Myeloma (2.19 ± 0.58 mPas), 142 with Drepanocytic Anemia (2.20 ± 0.79 mPas) showing statistically significant increases with respect to the characteristic values of the controls (1.68 ± 0.12mPas). Magnetic Relaxation is an option to evaluate plasma viscosity because it minimizes the volume of sample needed and eliminates the need to wash the viscometer between determinations. Magnetic Relaxation can compensate its relative high cost, compared with other Viscosimetry methods, facilitating other determinations of utility in several diseases.
VL  - 4
IS  - 2
ER  -

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