The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm

Kouam Foubi Brice Bertrand; Chuisseu Djamen Dieudonné Pascal; Dzeufiet Djomeni Paul Désiré; Samba Ngano Odette; Moifo Boniface; Zeh Odile Fernande; Guegang Goujou Emilienne; Mbede Maggy; Tiedeu Alain Bertin; Gonsu Fotsin Joseph

doi:doi:10.11648/j.ijbbmb.20160102.11

The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm

Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Moifo Boniface, Zeh Odile Fernande, Guegang Goujou Emilienne, Mbede Maggy, Tiedeu Alain Bertin, Gonsu Fotsin Joseph

Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 1, Issue 2)

Received: 22 October 2016 Accepted: 23 November 2016 Published: 5 January 2017

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Abstract

Irradiation results in a depression of the sperm count. The risk of damage to genes and chromosomal of spermatozoa exists and increases with increasing dose, potentially causing sterility or developments defects in children. Reputed to be a powerful natural antioxidant that may cause inhibition of radical processes, the protection of the macromolecules essential for cell survival, and limiting oxygen effect, Aged garlic extract (AGE) has been demonstrated to possess several physiological activities in experimental animals thus, the present study aimed to asses if AGE was able to modulate the effects of gamma radiation-induced injury to sperm count. 80 healthy male rats were randomly allotted according to the duration of the experiment into 2 lots (Lot 1: preventive aspect and Lot 2: Curative aspect) of 40 rats each. The animals of each Lot were randomly divided into four equally and double male rat groups, five rats each, among which, 20 irradiated and 20 Sham Irradiated. Sperm motility was appreciated the 8th day post irradiation by counting under a microscope the spermatozoa contained in a determined volume of homogenate of the tail of the epididymis and the sperm density determined using the cell MALASSEZ. Exposure of rats to gamma irradiation caused a significant disturbance in sperm count but in rats exposed to radiation then pre-treated and treated with AGE, the results showed an improvement. Our results prove that AGE has protective effects against radiation-induced changes in sperm count post irradiation. Thus, it could be concluded that AGE might reduce the biological hazards induced by gamma irradiation in rat’s sperm.

Published in	International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 1, Issue 2)
DOI	10.11648/j.ijbbmb.20160102.11
Page(s)	36-41
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Irradiation, Sperm, AGE, Epididymis, Rats

References

[1]	Yamini K, Gopal V. Natural radioprotective agents against Ionizing Radiation - An Overview. Int. J. Radiat. Biol. 2010; 2 (2): 1421-426.
[2]	Parihar V, Prabhakar K, Veerapur P, Kumar M, Reddy Y, Joshi R, et al Effect of radiation-induced cytotoxicity is in Swiss albino mice. Mutat Res. 2006; 611: 1-2, 9-16.
[3]	Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur, M. and Telser, JFree radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology. 2007; 39: 44-84.
[4]	Dragaric I, Dragaric Z. The radiation chemistry of water. Academic Press, New York, 1971, p. 256.
[5]	Scholes G. Radiation effects on DNA. Br J Radiol. 1983; 56 (664): 221-31.
[6]	Pradhan D, Nair C, Sreenivasan A. Radiation injury, repair and sensitization of microorganisms, Proc Natl Ind Acad Sci. 1973; 39: 516-30.
[7]	Little J. Radiation carcinogenesis. Carcinogenesis. 2000; 21 (3): 397-04.
[8]	Cerveny T. J., MacVittie T. J., Young R. W. Acute radiation syndrome in humans. Textbook of Military Medecine - Medical Consequences of Nuclear Warfare, Armed Forces Radiobiology Research Institute, Bethesda, Maryland, 1989; 17-36.
[9]	Donnelly EH, Nemhauser JB, Smith JM. "Acute radiation syndrome: assessment and management". South. Med. J. 2010; 103 (6): 541–6.
[10]	Armin Ansari. Radiation Threats and Your Safety: A Guide to Preparation and Response for Professionals and Community. 2009; p. 110.
[11]	Nair, C. K.; Salvi, V.; Kagiva, T. V. and Rajagopalan, R. Relevance of radioprotectors in radiotherapy studies with tocopherol monoglucoside. J. Environ. Pathol. Toxicol. Oncol. 2004; 23 (2): 153-160.
[12]	Patt H. M., Tyree E. B., Straube R. L., Smith D. E. Cysteine Protection against X Irradiation. Science. 1949; 110: 213.
[13]	Ramachandran L, Krishnan C, Nair C. Radioprotection by alpha-lipoïc acid palladium complex formulation (POLY-MVA) in mice. Cancer Biotherapy & Radiopharmaceuticals. 2010; 25: 395-399. PMID: 20701542.
[14]	Amagase H, Petesch B L, Matsuura H, Kasuga S and Itakura Y. “Intake of garlic and its bioactive components”. Journal of Nutrition. 2001; 131 (3) 955–962.
[15]	Balamash K, Albar O, Wang Q and Ahmed N: Effect of Kyolic® aged garlic extract on glycaemia, lipidaemia and oxidative stress in patients with type 2 diabetes mellitus. Journal of Diabetes Research and Clinical Metabolism. 2012; 1: p. 18.
[16]	EEC: Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Official Journal of the European Communities 1986; L358: 1–29.
[17]	Kameni Poumeni M. Evaluation aphrodisiac effects of aqueous extract of the flowers of lotus nymphaea on reproductive function of normal male rats with type 1 diabetes. Master thesis, animal physiology option. University of Yaounde I. Facebook sciences. 2011, p. 86.
[18]	Ngoula F, Watcho P, Dongmo M. C., Kenfack A., Kamtchouing P., Tchamboué J. Effects of Pirimiphos-methyl (an organophosphate insecticide) on the fertility of adult male rats. African Health Sciences, Makere University Medical School IDDN, 2007: 1680-6905.
[19]	Ashish Ranjan Singh, Kapil Singh, Preetam Singh Shekhawat. Spermicidal activity and antifertility activity of ethanolic extract of Withania somnifera in male albino rats. Int. J. Pharm. Sci. Rev. Res. 2013; 21 (2): 227-232.
[20]	W. H. O. Protocol MB -50: A method for examining the effect of the plant extracts administered orally on the fertility of male rats (APF/IP, 99914E), Geneva, 1983.
[21]	Bhosle S M, Huilgol N G and Mishra K P. Enhancement of radiation induced oxidative stress and cytotoxicity in tumor cells by ellagic acid. Clin. Chim. Acta. 2005; 359 (1-2): 89-100.
[22]	Soloviev A I, Stefanov A V, Tishkin S M, Khromov A S, Parshikov A V, Ivanova I V and Gurney A M. Saline containing phosphatidylecholine lipsomes possess the abilityto restore endothelial function damaged resulting from gamma irradiation. J. Physiol. Pharmacol. 2002; 53 (4-1): 701-12.
[23]	Tominaga, H.; Kodama, S.; Matsuda, N.; Suzuki, K. and Watanabe, M. Involvement of reactive oxygen species (ROS) in theinduction of genetic instability by radiation. J. Rad. Res. 2004; 45 (2): 181-8.
[24]	Dethier B., Wathelet J. P. et Hanon E. Contribution à l’étude de la synthèse de l’alline de l’ail. Master Bioingénieur en chimie et bio-industries, 2010, Grembloux Agro-Bio Tech et Université de Liège.
[25]	Kong Ah-Ng T, Yu R, Chen C, Mandlekar S, Primiano T. Signal transduction events elicited by natural products: Role of MAPK and caspase pathways in homeostatic response and induction of apoptosis. Arch. Pharm. Res. 2000; 23: 1-16.
[26]	Oakberg, E. F. Initial depletion and subsequent recovery of spermatogonia of the mouse after 20 r of gamma-rays and 100, 300, and 600 r of x-rays. Rad. Res. 1959; 11: 700-19.
[27]	Clifton, D. K. and Bremner, W. J. The effect of tesicular x irradiation on spermatogenesis in man. A comparison with mouse. J. Androl. 1983; 4 (6): 387-92.
[28]	Khaki A., Fathiazad F., Nouri M., Khaki A. A., Khameneki H. J., Hamadeh M. Evaluation of androgenic activity of Allium cepa on spermatogenesis in the rat. Folia Morphology. 2009; 68 (1): 45–51.

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Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Moifo Boniface, et al. (2017). The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm. International Journal of Biochemistry, Biophysics & Molecular Biology, 1(2), 36-41. https://doi.org/10.11648/j.ijbbmb.20160102.11

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ACS Style

Kouam Foubi Brice Bertrand; Chuisseu Djamen Dieudonné Pascal; Dzeufiet Djomeni Paul Désiré; Samba Ngano Odette; Moifo Boniface, et al. The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm. Int. J. Biochem. Biophys. Mol. Biol. 2017, 1(2), 36-41. doi: 10.11648/j.ijbbmb.20160102.11

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AMA Style

Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Moifo Boniface, et al. The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm. Int J Biochem Biophys Mol Biol. 2017;1(2):36-41. doi: 10.11648/j.ijbbmb.20160102.11

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@article{10.11648/j.ijbbmb.20160102.11,
  author = {Kouam Foubi Brice Bertrand and Chuisseu Djamen Dieudonné Pascal and Dzeufiet Djomeni Paul Désiré and Samba Ngano Odette and Moifo Boniface and Zeh Odile Fernande and Guegang Goujou Emilienne and Mbede Maggy and Tiedeu Alain Bertin and Gonsu Fotsin Joseph},
  title = {The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {1},
  number = {2},
  pages = {36-41},
  doi = {10.11648/j.ijbbmb.20160102.11},
  url = {https://doi.org/10.11648/j.ijbbmb.20160102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20160102.11},
  abstract = {Irradiation results in a depression of the sperm count. The risk of damage to genes and chromosomal of spermatozoa exists and increases with increasing dose, potentially causing sterility or developments defects in children. Reputed to be a powerful natural antioxidant that may cause inhibition of radical processes, the protection of the macromolecules essential for cell survival, and limiting oxygen effect, Aged garlic extract (AGE) has been demonstrated to possess several physiological activities in experimental animals thus, the present study aimed to asses if AGE was able to modulate the effects of gamma radiation-induced injury to sperm count. 80 healthy male rats were randomly allotted according to the duration of the experiment into 2 lots (Lot 1: preventive aspect and Lot 2: Curative aspect) of 40 rats each. The animals of each Lot were randomly divided into four equally and double male rat groups, five rats each, among which, 20 irradiated and 20 Sham Irradiated. Sperm motility was appreciated the 8th day post irradiation by counting under a microscope the spermatozoa contained in a determined volume of homogenate of the tail of the epididymis and the sperm density determined using the cell MALASSEZ. Exposure of rats to gamma irradiation caused a significant disturbance in sperm count but in rats exposed to radiation then pre-treated and treated with AGE, the results showed an improvement. Our results prove that AGE has protective effects against radiation-induced changes in sperm count post irradiation. Thus, it could be concluded that AGE might reduce the biological hazards induced by gamma irradiation in rat’s sperm.},
 year = {2017}
}

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TY  - JOUR
T1  - The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm
AU  - Kouam Foubi Brice Bertrand
AU  - Chuisseu Djamen Dieudonné Pascal
AU  - Dzeufiet Djomeni Paul Désiré
AU  - Samba Ngano Odette
AU  - Moifo Boniface
AU  - Zeh Odile Fernande
AU  - Guegang Goujou Emilienne
AU  - Mbede Maggy
AU  - Tiedeu Alain Bertin
AU  - Gonsu Fotsin Joseph
Y1  - 2017/01/05
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbbmb.20160102.11
DO  - 10.11648/j.ijbbmb.20160102.11
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 36
EP  - 41
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20160102.11
AB  - Irradiation results in a depression of the sperm count. The risk of damage to genes and chromosomal of spermatozoa exists and increases with increasing dose, potentially causing sterility or developments defects in children. Reputed to be a powerful natural antioxidant that may cause inhibition of radical processes, the protection of the macromolecules essential for cell survival, and limiting oxygen effect, Aged garlic extract (AGE) has been demonstrated to possess several physiological activities in experimental animals thus, the present study aimed to asses if AGE was able to modulate the effects of gamma radiation-induced injury to sperm count. 80 healthy male rats were randomly allotted according to the duration of the experiment into 2 lots (Lot 1: preventive aspect and Lot 2: Curative aspect) of 40 rats each. The animals of each Lot were randomly divided into four equally and double male rat groups, five rats each, among which, 20 irradiated and 20 Sham Irradiated. Sperm motility was appreciated the 8th day post irradiation by counting under a microscope the spermatozoa contained in a determined volume of homogenate of the tail of the epididymis and the sperm density determined using the cell MALASSEZ. Exposure of rats to gamma irradiation caused a significant disturbance in sperm count but in rats exposed to radiation then pre-treated and treated with AGE, the results showed an improvement. Our results prove that AGE has protective effects against radiation-induced changes in sperm count post irradiation. Thus, it could be concluded that AGE might reduce the biological hazards induced by gamma irradiation in rat’s sperm.
VL  - 1
IS  - 2
ER  -

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Author Information

Kouam Foubi Brice Bertrand

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Chuisseu Djamen Dieudonné Pascal

Faculty of Health Sciences, Université des Montagnes, Bangangté, Bangangté, Cameroon
Dzeufiet Djomeni Paul Désiré

Faculty of Sciences, University of Yaoundé I, Yaoundé, Cameroon
Samba Ngano Odette

Faculty of Sciences, University of Dschang, Dschang, Cameroon
Moifo Boniface

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Zeh Odile Fernande

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Guegang Goujou Emilienne

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Mbede Maggy

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon
Tiedeu Alain Bertin

National Advanced School of Engineering, University of Yaoundé I, Yaoundé, Cameroon
Gonsu Fotsin Joseph

Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon

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APA Style

Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Moifo Boniface, et al. (2017). The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm. International Journal of Biochemistry, Biophysics & Molecular Biology, 1(2), 36-41. https://doi.org/10.11648/j.ijbbmb.20160102.11

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ACS Style

Kouam Foubi Brice Bertrand; Chuisseu Djamen Dieudonné Pascal; Dzeufiet Djomeni Paul Désiré; Samba Ngano Odette; Moifo Boniface, et al. The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm. Int. J. Biochem. Biophys. Mol. Biol. 2017, 1(2), 36-41. doi: 10.11648/j.ijbbmb.20160102.11

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AMA Style

Kouam Foubi Brice Bertrand, Chuisseu Djamen Dieudonné Pascal, Dzeufiet Djomeni Paul Désiré, Samba Ngano Odette, Moifo Boniface, et al. The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm. Int J Biochem Biophys Mol Biol. 2017;1(2):36-41. doi: 10.11648/j.ijbbmb.20160102.11

Copy | Download

@article{10.11648/j.ijbbmb.20160102.11,
  author = {Kouam Foubi Brice Bertrand and Chuisseu Djamen Dieudonné Pascal and Dzeufiet Djomeni Paul Désiré and Samba Ngano Odette and Moifo Boniface and Zeh Odile Fernande and Guegang Goujou Emilienne and Mbede Maggy and Tiedeu Alain Bertin and Gonsu Fotsin Joseph},
  title = {The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {1},
  number = {2},
  pages = {36-41},
  doi = {10.11648/j.ijbbmb.20160102.11},
  url = {https://doi.org/10.11648/j.ijbbmb.20160102.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20160102.11},
  abstract = {Irradiation results in a depression of the sperm count. The risk of damage to genes and chromosomal of spermatozoa exists and increases with increasing dose, potentially causing sterility or developments defects in children. Reputed to be a powerful natural antioxidant that may cause inhibition of radical processes, the protection of the macromolecules essential for cell survival, and limiting oxygen effect, Aged garlic extract (AGE) has been demonstrated to possess several physiological activities in experimental animals thus, the present study aimed to asses if AGE was able to modulate the effects of gamma radiation-induced injury to sperm count. 80 healthy male rats were randomly allotted according to the duration of the experiment into 2 lots (Lot 1: preventive aspect and Lot 2: Curative aspect) of 40 rats each. The animals of each Lot were randomly divided into four equally and double male rat groups, five rats each, among which, 20 irradiated and 20 Sham Irradiated. Sperm motility was appreciated the 8th day post irradiation by counting under a microscope the spermatozoa contained in a determined volume of homogenate of the tail of the epididymis and the sperm density determined using the cell MALASSEZ. Exposure of rats to gamma irradiation caused a significant disturbance in sperm count but in rats exposed to radiation then pre-treated and treated with AGE, the results showed an improvement. Our results prove that AGE has protective effects against radiation-induced changes in sperm count post irradiation. Thus, it could be concluded that AGE might reduce the biological hazards induced by gamma irradiation in rat’s sperm.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - The Study of the Radiation Protection of Aged Garlic Extract to the Radiation Effects in Male Rat’s Sperm
AU  - Kouam Foubi Brice Bertrand
AU  - Chuisseu Djamen Dieudonné Pascal
AU  - Dzeufiet Djomeni Paul Désiré
AU  - Samba Ngano Odette
AU  - Moifo Boniface
AU  - Zeh Odile Fernande
AU  - Guegang Goujou Emilienne
AU  - Mbede Maggy
AU  - Tiedeu Alain Bertin
AU  - Gonsu Fotsin Joseph
Y1  - 2017/01/05
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbbmb.20160102.11
DO  - 10.11648/j.ijbbmb.20160102.11
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 36
EP  - 41
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20160102.11
AB  - Irradiation results in a depression of the sperm count. The risk of damage to genes and chromosomal of spermatozoa exists and increases with increasing dose, potentially causing sterility or developments defects in children. Reputed to be a powerful natural antioxidant that may cause inhibition of radical processes, the protection of the macromolecules essential for cell survival, and limiting oxygen effect, Aged garlic extract (AGE) has been demonstrated to possess several physiological activities in experimental animals thus, the present study aimed to asses if AGE was able to modulate the effects of gamma radiation-induced injury to sperm count. 80 healthy male rats were randomly allotted according to the duration of the experiment into 2 lots (Lot 1: preventive aspect and Lot 2: Curative aspect) of 40 rats each. The animals of each Lot were randomly divided into four equally and double male rat groups, five rats each, among which, 20 irradiated and 20 Sham Irradiated. Sperm motility was appreciated the 8th day post irradiation by counting under a microscope the spermatozoa contained in a determined volume of homogenate of the tail of the epididymis and the sperm density determined using the cell MALASSEZ. Exposure of rats to gamma irradiation caused a significant disturbance in sperm count but in rats exposed to radiation then pre-treated and treated with AGE, the results showed an improvement. Our results prove that AGE has protective effects against radiation-induced changes in sperm count post irradiation. Thus, it could be concluded that AGE might reduce the biological hazards induced by gamma irradiation in rat’s sperm.
VL  - 1
IS  - 2
ER  -

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