Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells

Hewida Hassan Fadel; Mohamed Ahmed Abdel Mohsen; Khaled El Sayed Soliman; Hanaa Mohamed Kohail; Shehata Mahmoud EL Sewedy

doi:doi:10.11648/j.crj.20180603.14

Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells

Hewida Hassan Fadel, Mohamed Ahmed Abdel Mohsen, Khaled El Sayed Soliman, Hanaa Mohamed Kohail, Shehata Mahmoud EL Sewedy

Published in Cancer Research Journal (Volume 6, Issue 3)

Received: 26 June 2018 Accepted: 9 July 2018 Published: 2 August 2018

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Abstract

In cancer patients, oxidative stress (OS) plays an important role in the initiation, promotion, progression of disease, in addition to, the continuous exposure to OS throughout the surgical procedure, chemotherapy and radiotherapy. The present study was designated to evaluate the role of vitamin D3 and B6 supplementation in combination with anti-cancer therapies in maintenance the redox status and their impact on angiogenesis, the main step in cancer progression and aggressiveness, as well as on the side effect of surgery, chemotherapy and/or radiotherapy. Our investigations were at two levels, firstly, assessment of chemical parameters for redox status and angiogenic factors, secondly, assessment level of circulating endothelial cells by applying immunoassay using antibodies coupling nanoparticles. Blood samples were collected once from 15 apparently healthy women (GP-I) and four times from 20 BC patients; (GpII)before mastectomy (B. M.), after mastectomy (A. M.), after ending of chemotherapy course (A. C.) and after 3 months later (A.3M) for both subgroups to Monitor the oxidant-antioxidants status (MDA and TAS) and angiogenic biomarkers (VEGF, ES and CECs). Our data reveals that comparing to control group, BC patients show significant elevation of MDA, VEGF and CECs (P<0.001) as well as ES (P<0.05). The serum level of CECs decreased significantly (P<0.001) in BC patients supplemented with vitamins from the beginning of chemotherapy comparing to those receiving chemotherapy alone and then supplemented with vitamins for three months later. Supplementation with vitamins D3 and B6 reduces the oxidative stress, the side effects of chemotherapy and radiotherapy as vomiting and fatigue, in addition to interfere with angiogenesis.

Published in	Cancer Research Journal (Volume 6, Issue 3)
DOI	10.11648/j.crj.20180603.14
Page(s)	92-100
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Breast Cancer, Vitamins D3 and B6, Angiogenesis, VEGF, ES, Circulating Endothelial Cells

References

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APA Style

Hewida Hassan Fadel, Mohamed Ahmed Abdel Mohsen, Khaled El Sayed Soliman, Hanaa Mohamed Kohail, Shehata Mahmoud EL Sewedy. (2018). Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells. Cancer Research Journal, 6(3), 92-100. https://doi.org/10.11648/j.crj.20180603.14

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ACS Style

Hewida Hassan Fadel; Mohamed Ahmed Abdel Mohsen; Khaled El Sayed Soliman; Hanaa Mohamed Kohail; Shehata Mahmoud EL Sewedy. Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells. Cancer Res. J. 2018, 6(3), 92-100. doi: 10.11648/j.crj.20180603.14

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AMA Style

Hewida Hassan Fadel, Mohamed Ahmed Abdel Mohsen, Khaled El Sayed Soliman, Hanaa Mohamed Kohail, Shehata Mahmoud EL Sewedy. Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells. Cancer Res J. 2018;6(3):92-100. doi: 10.11648/j.crj.20180603.14

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@article{10.11648/j.crj.20180603.14,
  author = {Hewida Hassan Fadel and Mohamed Ahmed Abdel Mohsen and Khaled El Sayed Soliman and Hanaa Mohamed Kohail and Shehata Mahmoud EL Sewedy},
  title = {Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells},
  journal = {Cancer Research Journal},
  volume = {6},
  number = {3},
  pages = {92-100},
  doi = {10.11648/j.crj.20180603.14},
  url = {https://doi.org/10.11648/j.crj.20180603.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20180603.14},
  abstract = {In cancer patients, oxidative stress (OS) plays an important role in the initiation, promotion, progression of disease, in addition to, the continuous exposure to OS throughout the surgical procedure, chemotherapy and radiotherapy. The present study was designated to evaluate the role of vitamin D3 and B6 supplementation in combination with anti-cancer therapies in maintenance the redox status and their impact on angiogenesis, the main step in cancer progression and aggressiveness, as well as on the side effect of surgery, chemotherapy and/or radiotherapy. Our investigations were at two levels, firstly, assessment of chemical parameters for redox status and angiogenic factors, secondly, assessment level of circulating endothelial cells by applying immunoassay using antibodies coupling nanoparticles. Blood samples were collected once from 15 apparently healthy women (GP-I) and four times from 20 BC patients; (GpII)before mastectomy (B. M.), after mastectomy (A. M.), after ending of chemotherapy course (A. C.) and after 3 months later (A.3M) for both subgroups to Monitor the oxidant-antioxidants status (MDA and TAS) and angiogenic biomarkers (VEGF, ES and CECs). Our data reveals that comparing to control group, BC patients show significant elevation of MDA, VEGF and CECs (PPP<0.001) in BC patients supplemented with vitamins from the beginning of chemotherapy comparing to those receiving chemotherapy alone and then supplemented with vitamins for three months later. Supplementation with vitamins D3 and B6 reduces the oxidative stress, the side effects of chemotherapy and radiotherapy as vomiting and fatigue, in addition to interfere with angiogenesis.},
 year = {2018}
}

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TY  - JOUR
T1  - Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells
AU  - Hewida Hassan Fadel
AU  - Mohamed Ahmed Abdel Mohsen
AU  - Khaled El Sayed Soliman
AU  - Hanaa Mohamed Kohail
AU  - Shehata Mahmoud EL Sewedy
Y1  - 2018/08/02
PY  - 2018
N1  - https://doi.org/10.11648/j.crj.20180603.14
DO  - 10.11648/j.crj.20180603.14
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 92
EP  - 100
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20180603.14
AB  - In cancer patients, oxidative stress (OS) plays an important role in the initiation, promotion, progression of disease, in addition to, the continuous exposure to OS throughout the surgical procedure, chemotherapy and radiotherapy. The present study was designated to evaluate the role of vitamin D3 and B6 supplementation in combination with anti-cancer therapies in maintenance the redox status and their impact on angiogenesis, the main step in cancer progression and aggressiveness, as well as on the side effect of surgery, chemotherapy and/or radiotherapy. Our investigations were at two levels, firstly, assessment of chemical parameters for redox status and angiogenic factors, secondly, assessment level of circulating endothelial cells by applying immunoassay using antibodies coupling nanoparticles. Blood samples were collected once from 15 apparently healthy women (GP-I) and four times from 20 BC patients; (GpII)before mastectomy (B. M.), after mastectomy (A. M.), after ending of chemotherapy course (A. C.) and after 3 months later (A.3M) for both subgroups to Monitor the oxidant-antioxidants status (MDA and TAS) and angiogenic biomarkers (VEGF, ES and CECs). Our data reveals that comparing to control group, BC patients show significant elevation of MDA, VEGF and CECs (PPP<0.001) in BC patients supplemented with vitamins from the beginning of chemotherapy comparing to those receiving chemotherapy alone and then supplemented with vitamins for three months later. Supplementation with vitamins D3 and B6 reduces the oxidative stress, the side effects of chemotherapy and radiotherapy as vomiting and fatigue, in addition to interfere with angiogenesis.
VL  - 6
IS  - 3
ER  -

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Author Information

Hewida Hassan Fadel

Medical Laboratory Technology Department/ Faculty of Allied Medical Science, Pharos University in Alexandria, Alexandria, Egypt
Mohamed Ahmed Abdel Mohsen

Applied Medical Chemistry Department/ Medical Research Institute, University of Alexandria, Alexandria, Egypt
Khaled El Sayed Soliman

Experimental and Clinical Surgery Department/ Hospital of Medical Research Institute, University of Alexandria, Alexandria, Egypt
Hanaa Mohamed Kohail

Cancer Management and Research Department/ Hospital of Medical Research Institute, University of Alexandria, Alexandria, Egypt
Shehata Mahmoud EL Sewedy

Medical Laboratory Technology Department/ Faculty of Allied Medical Science, Pharos University in Alexandria, Alexandria, Egypt

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APA Style

Hewida Hassan Fadel, Mohamed Ahmed Abdel Mohsen, Khaled El Sayed Soliman, Hanaa Mohamed Kohail, Shehata Mahmoud EL Sewedy. (2018). Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells. Cancer Research Journal, 6(3), 92-100. https://doi.org/10.11648/j.crj.20180603.14

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ACS Style

Hewida Hassan Fadel; Mohamed Ahmed Abdel Mohsen; Khaled El Sayed Soliman; Hanaa Mohamed Kohail; Shehata Mahmoud EL Sewedy. Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells. Cancer Res. J. 2018, 6(3), 92-100. doi: 10.11648/j.crj.20180603.14

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AMA Style

Hewida Hassan Fadel, Mohamed Ahmed Abdel Mohsen, Khaled El Sayed Soliman, Hanaa Mohamed Kohail, Shehata Mahmoud EL Sewedy. Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells. Cancer Res J. 2018;6(3):92-100. doi: 10.11648/j.crj.20180603.14

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@article{10.11648/j.crj.20180603.14,
  author = {Hewida Hassan Fadel and Mohamed Ahmed Abdel Mohsen and Khaled El Sayed Soliman and Hanaa Mohamed Kohail and Shehata Mahmoud EL Sewedy},
  title = {Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells},
  journal = {Cancer Research Journal},
  volume = {6},
  number = {3},
  pages = {92-100},
  doi = {10.11648/j.crj.20180603.14},
  url = {https://doi.org/10.11648/j.crj.20180603.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20180603.14},
  abstract = {In cancer patients, oxidative stress (OS) plays an important role in the initiation, promotion, progression of disease, in addition to, the continuous exposure to OS throughout the surgical procedure, chemotherapy and radiotherapy. The present study was designated to evaluate the role of vitamin D3 and B6 supplementation in combination with anti-cancer therapies in maintenance the redox status and their impact on angiogenesis, the main step in cancer progression and aggressiveness, as well as on the side effect of surgery, chemotherapy and/or radiotherapy. Our investigations were at two levels, firstly, assessment of chemical parameters for redox status and angiogenic factors, secondly, assessment level of circulating endothelial cells by applying immunoassay using antibodies coupling nanoparticles. Blood samples were collected once from 15 apparently healthy women (GP-I) and four times from 20 BC patients; (GpII)before mastectomy (B. M.), after mastectomy (A. M.), after ending of chemotherapy course (A. C.) and after 3 months later (A.3M) for both subgroups to Monitor the oxidant-antioxidants status (MDA and TAS) and angiogenic biomarkers (VEGF, ES and CECs). Our data reveals that comparing to control group, BC patients show significant elevation of MDA, VEGF and CECs (PPP<0.001) in BC patients supplemented with vitamins from the beginning of chemotherapy comparing to those receiving chemotherapy alone and then supplemented with vitamins for three months later. Supplementation with vitamins D3 and B6 reduces the oxidative stress, the side effects of chemotherapy and radiotherapy as vomiting and fatigue, in addition to interfere with angiogenesis.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Study Effect of Vitamins D3 and B6 Supplementation on Treatment Response in Breast Cancer Using Antibody-Coated Magnetic Nanoparticles Immunoassay for Circulating Endothelial Cells
AU  - Hewida Hassan Fadel
AU  - Mohamed Ahmed Abdel Mohsen
AU  - Khaled El Sayed Soliman
AU  - Hanaa Mohamed Kohail
AU  - Shehata Mahmoud EL Sewedy
Y1  - 2018/08/02
PY  - 2018
N1  - https://doi.org/10.11648/j.crj.20180603.14
DO  - 10.11648/j.crj.20180603.14
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 92
EP  - 100
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20180603.14
AB  - In cancer patients, oxidative stress (OS) plays an important role in the initiation, promotion, progression of disease, in addition to, the continuous exposure to OS throughout the surgical procedure, chemotherapy and radiotherapy. The present study was designated to evaluate the role of vitamin D3 and B6 supplementation in combination with anti-cancer therapies in maintenance the redox status and their impact on angiogenesis, the main step in cancer progression and aggressiveness, as well as on the side effect of surgery, chemotherapy and/or radiotherapy. Our investigations were at two levels, firstly, assessment of chemical parameters for redox status and angiogenic factors, secondly, assessment level of circulating endothelial cells by applying immunoassay using antibodies coupling nanoparticles. Blood samples were collected once from 15 apparently healthy women (GP-I) and four times from 20 BC patients; (GpII)before mastectomy (B. M.), after mastectomy (A. M.), after ending of chemotherapy course (A. C.) and after 3 months later (A.3M) for both subgroups to Monitor the oxidant-antioxidants status (MDA and TAS) and angiogenic biomarkers (VEGF, ES and CECs). Our data reveals that comparing to control group, BC patients show significant elevation of MDA, VEGF and CECs (PPP<0.001) in BC patients supplemented with vitamins from the beginning of chemotherapy comparing to those receiving chemotherapy alone and then supplemented with vitamins for three months later. Supplementation with vitamins D3 and B6 reduces the oxidative stress, the side effects of chemotherapy and radiotherapy as vomiting and fatigue, in addition to interfere with angiogenesis.
VL  - 6
IS  - 3
ER  -

Copy | Download