Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria

Melhag Mohamed; Kerroucha Rabah; Melouli Hamid; Midoun Nori; Zemour Lakhdar; Ayyach Ghassan; Asselah Fatima; Bendissari Kheira; EL Kebir Fatima Zohra

doi:doi:10.11648/j.crj.20180603.13

Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria

Melhag Mohamed, Kerroucha Rabah, Melouli Hamid, Midoun Nori, Zemour Lakhdar, Ayyach Ghassan, Asselah Fatima, Bendissari Kheira, EL Kebir Fatima Zohra

Published in Cancer Research Journal (Volume 6, Issue 3)

Received: 28 May 2018 Accepted: 2 July 2018 Published: 27 July 2018

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Abstract

To evaluate and compare the performance of PCR (polymerase chain reaction) and second-generation capture hybridization (HC2) in the detection of high-risk Human Papillomavirus (HPV-HR) and to estimate their prevalence among normal cervical, dysplastic and cervical cancer in the Algerian population. 264 women were subjected to cyto-histological and colposcopic diagnostics and to the HPV test by PCR and Hybrid capture 2. The search for HPV DNA by conventional PCR was carried out with MY09 / 11 consensus primers and the typing of HPV with the hybridization technique in solution, Digene Sharp Signal for PCR. Hybrid Capture® 2 was performed with the digene HPV HC2 DNA Test kit. The PCR and HC2 performance tests included sensitivity, specificity, positive and negative predictive values, Youden's index, and Yule's Q coefficient. HPV-HR research has shown that these viruses are associated with 100% high-grade lesions, 88.2% by PCR and 83.3% by HC2 (p = 0.8) with invasive cancer. The PCR and HC2 performance tests were in favor of HCV HPV testing which was more sensitive, more specific, more predictive and more effective. The results on the prevalence of HPV-HR associated with cervical lesions are comparable to those in the literature. In comparison with PCR, the capture hybridization technique 2 is recommended for HPV diagnosis because of its better performance and simplicity.

Published in	Cancer Research Journal (Volume 6, Issue 3)
DOI	10.11648/j.crj.20180603.13
Page(s)	84-91
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Cervical Cancer, Precancerous Lesions, Prevalence, HPV Testing, PCR, Capture Hybridization 2

References

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APA Style

Melhag Mohamed, Kerroucha Rabah, Melouli Hamid, Midoun Nori, Zemour Lakhdar, et al. (2018). Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria. Cancer Research Journal, 6(3), 84-91. https://doi.org/10.11648/j.crj.20180603.13

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ACS Style

Melhag Mohamed; Kerroucha Rabah; Melouli Hamid; Midoun Nori; Zemour Lakhdar, et al. Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria. Cancer Res. J. 2018, 6(3), 84-91. doi: 10.11648/j.crj.20180603.13

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AMA Style

Melhag Mohamed, Kerroucha Rabah, Melouli Hamid, Midoun Nori, Zemour Lakhdar, et al. Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria. Cancer Res J. 2018;6(3):84-91. doi: 10.11648/j.crj.20180603.13

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@article{10.11648/j.crj.20180603.13,
  author = {Melhag Mohamed and Kerroucha Rabah and Melouli Hamid and Midoun Nori and Zemour Lakhdar and Ayyach Ghassan and Asselah Fatima and Bendissari Kheira and EL Kebir Fatima Zohra},
  title = {Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria},
  journal = {Cancer Research Journal},
  volume = {6},
  number = {3},
  pages = {84-91},
  doi = {10.11648/j.crj.20180603.13},
  url = {https://doi.org/10.11648/j.crj.20180603.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20180603.13},
  abstract = {To evaluate and compare the performance of PCR (polymerase chain reaction) and second-generation capture hybridization (HC2) in the detection of high-risk Human Papillomavirus (HPV-HR) and to estimate their prevalence among normal cervical, dysplastic and cervical cancer in the Algerian population. 264 women were subjected to cyto-histological and colposcopic diagnostics and to the HPV test by PCR and Hybrid capture 2. The search for HPV DNA by conventional PCR was carried out with MY09 / 11 consensus primers and the typing of HPV with the hybridization technique in solution, Digene Sharp Signal for PCR. Hybrid Capture® 2 was performed with the digene HPV HC2 DNA Test kit. The PCR and HC2 performance tests included sensitivity, specificity, positive and negative predictive values, Youden's index, and Yule's Q coefficient. HPV-HR research has shown that these viruses are associated with 100% high-grade lesions, 88.2% by PCR and 83.3% by HC2 (p = 0.8) with invasive cancer. The PCR and HC2 performance tests were in favor of HCV HPV testing which was more sensitive, more specific, more predictive and more effective. The results on the prevalence of HPV-HR associated with cervical lesions are comparable to those in the literature. In comparison with PCR, the capture hybridization technique 2 is recommended for HPV diagnosis because of its better performance and simplicity.},
 year = {2018}
}

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TY  - JOUR
T1  - Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria
AU  - Melhag Mohamed
AU  - Kerroucha Rabah
AU  - Melouli Hamid
AU  - Midoun Nori
AU  - Zemour Lakhdar
AU  - Ayyach Ghassan
AU  - Asselah Fatima
AU  - Bendissari Kheira
AU  - EL Kebir Fatima Zohra
Y1  - 2018/07/27
PY  - 2018
N1  - https://doi.org/10.11648/j.crj.20180603.13
DO  - 10.11648/j.crj.20180603.13
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 84
EP  - 91
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20180603.13
AB  - To evaluate and compare the performance of PCR (polymerase chain reaction) and second-generation capture hybridization (HC2) in the detection of high-risk Human Papillomavirus (HPV-HR) and to estimate their prevalence among normal cervical, dysplastic and cervical cancer in the Algerian population. 264 women were subjected to cyto-histological and colposcopic diagnostics and to the HPV test by PCR and Hybrid capture 2. The search for HPV DNA by conventional PCR was carried out with MY09 / 11 consensus primers and the typing of HPV with the hybridization technique in solution, Digene Sharp Signal for PCR. Hybrid Capture® 2 was performed with the digene HPV HC2 DNA Test kit. The PCR and HC2 performance tests included sensitivity, specificity, positive and negative predictive values, Youden's index, and Yule's Q coefficient. HPV-HR research has shown that these viruses are associated with 100% high-grade lesions, 88.2% by PCR and 83.3% by HC2 (p = 0.8) with invasive cancer. The PCR and HC2 performance tests were in favor of HCV HPV testing which was more sensitive, more specific, more predictive and more effective. The results on the prevalence of HPV-HR associated with cervical lesions are comparable to those in the literature. In comparison with PCR, the capture hybridization technique 2 is recommended for HPV diagnosis because of its better performance and simplicity.
VL  - 6
IS  - 3
ER  -

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Author Information

Melhag Mohamed

Biology Department, Faculty of Science of Nature and Life, University of Oran 1 Ahmed Ben Bella, Oran, Algeria
Kerroucha Rabah

Biology Department, Faculty of Science of Nature and Life, University of Oran 1 Ahmed Ben Bella, Oran, Algeria
Melouli Hamid

Institut Pasteur of Algeria, Algiers, Algeria
Midoun Nori

Department of Epidemiology and Preventive Medicine, EHU of Oran, Oran, Algeria
Zemour Lakhdar

Department of Epidemiology and Preventive Medicine, EHU of Oran, Oran, Algeria
Ayyach Ghassan

Gynecology-Obstetrics Department. EPH of Zeralda, Algiers Algeria
Asselah Fatima

Pathology Department, Mustapha Pasha University Hospital, Algiers, Algeria
Bendissari Kheira

Pathology Department, Beni Messous University Hospital, Algiers, Algeria
EL Kebir Fatima Zohra

Biology Department, Faculty of Science of Nature and Life, University of Oran 1 Ahmed Ben Bella, Oran, Algeria

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Melhag Mohamed, Kerroucha Rabah, Melouli Hamid, Midoun Nori, Zemour Lakhdar, et al. (2018). Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria. Cancer Research Journal, 6(3), 84-91. https://doi.org/10.11648/j.crj.20180603.13

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ACS Style

Melhag Mohamed; Kerroucha Rabah; Melouli Hamid; Midoun Nori; Zemour Lakhdar, et al. Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria. Cancer Res. J. 2018, 6(3), 84-91. doi: 10.11648/j.crj.20180603.13

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AMA Style

Melhag Mohamed, Kerroucha Rabah, Melouli Hamid, Midoun Nori, Zemour Lakhdar, et al. Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria. Cancer Res J. 2018;6(3):84-91. doi: 10.11648/j.crj.20180603.13

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@article{10.11648/j.crj.20180603.13,
  author = {Melhag Mohamed and Kerroucha Rabah and Melouli Hamid and Midoun Nori and Zemour Lakhdar and Ayyach Ghassan and Asselah Fatima and Bendissari Kheira and EL Kebir Fatima Zohra},
  title = {Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria},
  journal = {Cancer Research Journal},
  volume = {6},
  number = {3},
  pages = {84-91},
  doi = {10.11648/j.crj.20180603.13},
  url = {https://doi.org/10.11648/j.crj.20180603.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20180603.13},
  abstract = {To evaluate and compare the performance of PCR (polymerase chain reaction) and second-generation capture hybridization (HC2) in the detection of high-risk Human Papillomavirus (HPV-HR) and to estimate their prevalence among normal cervical, dysplastic and cervical cancer in the Algerian population. 264 women were subjected to cyto-histological and colposcopic diagnostics and to the HPV test by PCR and Hybrid capture 2. The search for HPV DNA by conventional PCR was carried out with MY09 / 11 consensus primers and the typing of HPV with the hybridization technique in solution, Digene Sharp Signal for PCR. Hybrid Capture® 2 was performed with the digene HPV HC2 DNA Test kit. The PCR and HC2 performance tests included sensitivity, specificity, positive and negative predictive values, Youden's index, and Yule's Q coefficient. HPV-HR research has shown that these viruses are associated with 100% high-grade lesions, 88.2% by PCR and 83.3% by HC2 (p = 0.8) with invasive cancer. The PCR and HC2 performance tests were in favor of HCV HPV testing which was more sensitive, more specific, more predictive and more effective. The results on the prevalence of HPV-HR associated with cervical lesions are comparable to those in the literature. In comparison with PCR, the capture hybridization technique 2 is recommended for HPV diagnosis because of its better performance and simplicity.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Evaluation of the Performance of PCR and Hybrid Capture 2 in the Detection of Human Papillomavirus Associated with Cervical Cancer in Algeria
AU  - Melhag Mohamed
AU  - Kerroucha Rabah
AU  - Melouli Hamid
AU  - Midoun Nori
AU  - Zemour Lakhdar
AU  - Ayyach Ghassan
AU  - Asselah Fatima
AU  - Bendissari Kheira
AU  - EL Kebir Fatima Zohra
Y1  - 2018/07/27
PY  - 2018
N1  - https://doi.org/10.11648/j.crj.20180603.13
DO  - 10.11648/j.crj.20180603.13
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 84
EP  - 91
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20180603.13
AB  - To evaluate and compare the performance of PCR (polymerase chain reaction) and second-generation capture hybridization (HC2) in the detection of high-risk Human Papillomavirus (HPV-HR) and to estimate their prevalence among normal cervical, dysplastic and cervical cancer in the Algerian population. 264 women were subjected to cyto-histological and colposcopic diagnostics and to the HPV test by PCR and Hybrid capture 2. The search for HPV DNA by conventional PCR was carried out with MY09 / 11 consensus primers and the typing of HPV with the hybridization technique in solution, Digene Sharp Signal for PCR. Hybrid Capture® 2 was performed with the digene HPV HC2 DNA Test kit. The PCR and HC2 performance tests included sensitivity, specificity, positive and negative predictive values, Youden's index, and Yule's Q coefficient. HPV-HR research has shown that these viruses are associated with 100% high-grade lesions, 88.2% by PCR and 83.3% by HC2 (p = 0.8) with invasive cancer. The PCR and HC2 performance tests were in favor of HCV HPV testing which was more sensitive, more specific, more predictive and more effective. The results on the prevalence of HPV-HR associated with cervical lesions are comparable to those in the literature. In comparison with PCR, the capture hybridization technique 2 is recommended for HPV diagnosis because of its better performance and simplicity.
VL  - 6
IS  - 3
ER  -

Copy | Download