Evaluation of Bioethanol Production from Enset (<i>Ensete ventricosum</i> (Welw.) Cheesman) Processing Waste and Leaf Using <i>Saccharomyces cerevisiae</i>

Desta Lamore Erebo

doi:doi:10.11648/j.be.20210502.13

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Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae

Desta Lamore Erebo

Published in Bioprocess Engineering (Volume 5, Issue 2)

Received: 10 September 2021 Accepted: 19 October 2021 Published: 5 November 2021

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Abstract

The depletion of fossil fuel reserves and the increasing awareness of greenhouse gas emissions act as the primary driving force for finding alternative renewable energy sources, especially those derived from biomass. This study was conducted with the objective of bio-ethanol production from non- edible plant biomass, Enset [Ensete ventricosum (Welw.) Cheesman] processing waste and leaves in batch fermentation using yeas (S. cerevisiae). Forty grams of acid pre-treated or untreated ensete processing waste and leaves were incubated with 1% of yeast under three different temperature (26°C, 30°C and 40°C) treatments and allowed to ferment over 16 days. Yeast cell density, total reducing sugars and percent of ethanol was measured at 4 days interval spectrophotometrically beginning from the incubation period. Results showed that all measured parameters subsequently decrease with increasing days of fermentation in both acid pre-treated and untreated substrates. Acid pre-treated substrates resulted in higher amounts of cell density, total reducing sugars and bio-ethanol than untreated substrates. Among the different temperatures, 30°C produced more bio-ethanol than others throughout the fermentation periods, and the result was in agreement with cell density and total sugars measured. In conclusion, this study showed that Enset processing waste and leaves can be used as a feedstock for bio-ethanol production and the yield can be increased with acid pre-treatment and incubation under 30°C temperature.

Published in	Bioprocess Engineering (Volume 5, Issue 2)
DOI	10.11648/j.be.20210502.13
Page(s)	49-55
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Batch Fermentation, Cell Biomass, Bio-ethanol, Enset Processing Waste, Ensete Leaf, Reducing Sugar

References

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APA Style

Desta Lamore Erebo. (2021). Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae. Bioprocess Engineering, 5(2), 49-55. https://doi.org/10.11648/j.be.20210502.13

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ACS Style

Desta Lamore Erebo. Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae. Bioprocess Eng. 2021, 5(2), 49-55. doi: 10.11648/j.be.20210502.13

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AMA Style

Desta Lamore Erebo. Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae. Bioprocess Eng. 2021;5(2):49-55. doi: 10.11648/j.be.20210502.13

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@article{10.11648/j.be.20210502.13,
  author = {Desta Lamore Erebo},
  title = {Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae},
  journal = {Bioprocess Engineering},
  volume = {5},
  number = {2},
  pages = {49-55},
  doi = {10.11648/j.be.20210502.13},
  url = {https://doi.org/10.11648/j.be.20210502.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20210502.13},
  abstract = {The depletion of fossil fuel reserves and the increasing awareness of greenhouse gas emissions act as the primary driving force for finding alternative renewable energy sources, especially those derived from biomass. This study was conducted with the objective of bio-ethanol production from non- edible plant biomass, Enset [Ensete ventricosum (Welw.) Cheesman] processing waste and leaves in batch fermentation using yeas (S. cerevisiae). Forty grams of acid pre-treated or untreated ensete processing waste and leaves were incubated with 1% of yeast under three different temperature (26°C, 30°C and 40°C) treatments and allowed to ferment over 16 days. Yeast cell density, total reducing sugars and percent of ethanol was measured at 4 days interval spectrophotometrically beginning from the incubation period. Results showed that all measured parameters subsequently decrease with increasing days of fermentation in both acid pre-treated and untreated substrates. Acid pre-treated substrates resulted in higher amounts of cell density, total reducing sugars and bio-ethanol than untreated substrates. Among the different temperatures, 30°C produced more bio-ethanol than others throughout the fermentation periods, and the result was in agreement with cell density and total sugars measured. In conclusion, this study showed that Enset processing waste and leaves can be used as a feedstock for bio-ethanol production and the yield can be increased with acid pre-treatment and incubation under 30°C temperature.},
 year = {2021}
}

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TY  - JOUR
T1  - Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae
AU  - Desta Lamore Erebo
Y1  - 2021/11/05
PY  - 2021
N1  - https://doi.org/10.11648/j.be.20210502.13
DO  - 10.11648/j.be.20210502.13
T2  - Bioprocess Engineering
JF  - Bioprocess Engineering
JO  - Bioprocess Engineering
SP  - 49
EP  - 55
PB  - Science Publishing Group
SN  - 2578-8701
UR  - https://doi.org/10.11648/j.be.20210502.13
AB  - The depletion of fossil fuel reserves and the increasing awareness of greenhouse gas emissions act as the primary driving force for finding alternative renewable energy sources, especially those derived from biomass. This study was conducted with the objective of bio-ethanol production from non- edible plant biomass, Enset [Ensete ventricosum (Welw.) Cheesman] processing waste and leaves in batch fermentation using yeas (S. cerevisiae). Forty grams of acid pre-treated or untreated ensete processing waste and leaves were incubated with 1% of yeast under three different temperature (26°C, 30°C and 40°C) treatments and allowed to ferment over 16 days. Yeast cell density, total reducing sugars and percent of ethanol was measured at 4 days interval spectrophotometrically beginning from the incubation period. Results showed that all measured parameters subsequently decrease with increasing days of fermentation in both acid pre-treated and untreated substrates. Acid pre-treated substrates resulted in higher amounts of cell density, total reducing sugars and bio-ethanol than untreated substrates. Among the different temperatures, 30°C produced more bio-ethanol than others throughout the fermentation periods, and the result was in agreement with cell density and total sugars measured. In conclusion, this study showed that Enset processing waste and leaves can be used as a feedstock for bio-ethanol production and the yield can be increased with acid pre-treatment and incubation under 30°C temperature.
VL  - 5
IS  - 2
ER  -

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Author Information

Desta Lamore Erebo

Biotechnology Department, College of Natural and Computational Sciences, Wachemo University, Hossana, Ethiopia

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Desta Lamore Erebo. (2021). Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae. Bioprocess Engineering, 5(2), 49-55. https://doi.org/10.11648/j.be.20210502.13

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ACS Style

Desta Lamore Erebo. Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae. Bioprocess Eng. 2021, 5(2), 49-55. doi: 10.11648/j.be.20210502.13

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AMA Style

Desta Lamore Erebo. Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae. Bioprocess Eng. 2021;5(2):49-55. doi: 10.11648/j.be.20210502.13

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@article{10.11648/j.be.20210502.13,
  author = {Desta Lamore Erebo},
  title = {Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae},
  journal = {Bioprocess Engineering},
  volume = {5},
  number = {2},
  pages = {49-55},
  doi = {10.11648/j.be.20210502.13},
  url = {https://doi.org/10.11648/j.be.20210502.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20210502.13},
  abstract = {The depletion of fossil fuel reserves and the increasing awareness of greenhouse gas emissions act as the primary driving force for finding alternative renewable energy sources, especially those derived from biomass. This study was conducted with the objective of bio-ethanol production from non- edible plant biomass, Enset [Ensete ventricosum (Welw.) Cheesman] processing waste and leaves in batch fermentation using yeas (S. cerevisiae). Forty grams of acid pre-treated or untreated ensete processing waste and leaves were incubated with 1% of yeast under three different temperature (26°C, 30°C and 40°C) treatments and allowed to ferment over 16 days. Yeast cell density, total reducing sugars and percent of ethanol was measured at 4 days interval spectrophotometrically beginning from the incubation period. Results showed that all measured parameters subsequently decrease with increasing days of fermentation in both acid pre-treated and untreated substrates. Acid pre-treated substrates resulted in higher amounts of cell density, total reducing sugars and bio-ethanol than untreated substrates. Among the different temperatures, 30°C produced more bio-ethanol than others throughout the fermentation periods, and the result was in agreement with cell density and total sugars measured. In conclusion, this study showed that Enset processing waste and leaves can be used as a feedstock for bio-ethanol production and the yield can be increased with acid pre-treatment and incubation under 30°C temperature.},
 year = {2021}
}

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TY  - JOUR
T1  - Evaluation of Bioethanol Production from Enset (Ensete ventricosum (Welw.) Cheesman) Processing Waste and Leaf Using Saccharomyces cerevisiae
AU  - Desta Lamore Erebo
Y1  - 2021/11/05
PY  - 2021
N1  - https://doi.org/10.11648/j.be.20210502.13
DO  - 10.11648/j.be.20210502.13
T2  - Bioprocess Engineering
JF  - Bioprocess Engineering
JO  - Bioprocess Engineering
SP  - 49
EP  - 55
PB  - Science Publishing Group
SN  - 2578-8701
UR  - https://doi.org/10.11648/j.be.20210502.13
AB  - The depletion of fossil fuel reserves and the increasing awareness of greenhouse gas emissions act as the primary driving force for finding alternative renewable energy sources, especially those derived from biomass. This study was conducted with the objective of bio-ethanol production from non- edible plant biomass, Enset [Ensete ventricosum (Welw.) Cheesman] processing waste and leaves in batch fermentation using yeas (S. cerevisiae). Forty grams of acid pre-treated or untreated ensete processing waste and leaves were incubated with 1% of yeast under three different temperature (26°C, 30°C and 40°C) treatments and allowed to ferment over 16 days. Yeast cell density, total reducing sugars and percent of ethanol was measured at 4 days interval spectrophotometrically beginning from the incubation period. Results showed that all measured parameters subsequently decrease with increasing days of fermentation in both acid pre-treated and untreated substrates. Acid pre-treated substrates resulted in higher amounts of cell density, total reducing sugars and bio-ethanol than untreated substrates. Among the different temperatures, 30°C produced more bio-ethanol than others throughout the fermentation periods, and the result was in agreement with cell density and total sugars measured. In conclusion, this study showed that Enset processing waste and leaves can be used as a feedstock for bio-ethanol production and the yield can be increased with acid pre-treatment and incubation under 30°C temperature.
VL  - 5
IS  - 2
ER  -

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