Determination of Oleanolic Acid Content in <i>Radix Polysciacis</i> in Tra Vinh Province

Le Thi Minh Thu; Pham Hoang Anh

doi:doi:10.11648/j.aas.20230803.16

| Peer-Reviewed

Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province

Le Thi Minh Thu, Pham Hoang Anh

Published in Advances in Applied Sciences (Volume 8, Issue 3)

Received: 4 August 2023 Accepted: 25 August 2023 Published: 8 September 2023

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Polyscias fruticosa (L.) Harms is a traditional medicine, which is planted widely in Tra Vinh province of Vietnam. It is used for nourishing the body, curing asthenia, emaciation, fatigue, poor digestion, poor metabolism, enhance working capacity and brain activity. Oleanolic acid (OA), a marker, is a sapogenin in radix polysciacis. Quantification of OA by high performance liquid chromatography (HPLC) makes an important contribution to the quality assessment of the medicinal plant of Polyscias fruticosa (L.) Harms. In order to develop and apply a method to evaluate the content OA in radix polysciacis, planted in Tra Vinh province, many conditions for extracting OA from radix polysciacis were investigated; many HPLC testing conditions were performed find a simple, accurate and fast analytical procedure for the quantification of OA in extract solution of radix polysciacis. As a result, the optimum extraction procedure of OA from the roots of Polyscias fruticosa (L.) Harms was found out; a simple, accurate and fast analytical procedure for the quantification of OA in radix polysciacis’s extract solution was developed, evaluated and applied to assets the OA content of 05 samples of radix polysciacis collected in different regions of Tra Vinh province. This developed method can control the quality of medicinal materials of Polyscias fruticosa (L.) Harms and its preparation effectively.

Published in	Advances in Applied Sciences (Volume 8, Issue 3)
DOI	10.11648/j.aas.20230803.16
Page(s)	100-105
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Polyscias fruticosa (L.) Harms, Oleanolic Acid, HPLC

References

[1]	Vo Van Chi. (2012). Dictionary of Vietnamese Medicinal Plants; Publisher of Medicine: Hanoi, Vietnam, p. 937-938.
[2]	Do Tat Loi. (2013). Medicinal Plants and Remedy of Vietnam; Publisher of Hong Duc: Hanoi, Vietnam, p. 828-830.
[3]	Asumeng Koffuor G, Boye A, Kyei S, Ofori-Amoah J, Akomanin Asiamah E, Barku A, Acheampong J, Amegashie E, Kumi Awuku A. (2014). Anti-inflammatory and safety assessment of Polyscias fruticosa (L.) Harms leaf extract in ovalbumin-induced asthma. Pharm Biol. 2014; 3 (5); p. 337-342.
[4]	M. P. Nguyen. (2020). Impact of roasting to total phenolic, flavonoid and antioxidant activities in root, bark and leaf of Polyscias fruticose. Journal of Pharmaceutical Research International; 32 (2), p. 13–17.
[5]	Koffuor, G. A.; Boye, A.; Kyei, S.; Ofori-Amoah, J.; Asiamah, E. A.; Barku, A.; Acheampong, J.; Amegashie, E.; Awuku, A. K. (2016). Anti-asthmatic property and possible mode of activity of an ethanol leaf extract of Polyscias fruticose. Pharm. Biol. 54, 1354–1363.
[6]	Khoang, L. T.; Huyen, H. T. T.; Chung, H. V.; Duy, L. X.; Toan, T. Q.; Bich, H. T.; Minh, P. T. H.; Pham, D. T. N.; Hien, T. T. Optimization of Total Saponin Extraction from Polyscias fruticosa Roots Using the Ultrasonic-Assisted Method and Response Surface Methodology. Processes 2022, 10, 2034. https://doi.org/10.3390/pr10102034
[7]	Ly, H. T.; Nguyen, T. T. H.; Le, V. M.; Lam, B. T.; Mai, T. T. T.; Dang, T. P. T. (2022). Therapeutic potential of Polyscias fruticosa (L.) Harms leaf extract for parkinson’s disease treatment by Drosophila melanogaster model. Oxid. Med. Cell. Longev. 2022, 5262677.
[8]	Peng Xu, Bioa Yu, Chapter One - Chemical synthesis of saponins: An update, Editor (s): David C. Baker, Advances in Carbohydrate Chemistry and Biochemistry, Academic Press, Volume 79, 2021, p. 1-62, https://doi.org/10.1016/bs.accb.2021.11.001.
[9]	Ashmawy, N. S., Gad, H. A., Ashour, M. L., El-Ahmady, S. H., & Singab, A. N. B. (2020). The genus Polyscias (Araliaceae): A phytochemical and biological review. Journal of Herbal Medicine, vol 23, 100377. doi: 10.1016/j.hermed.2020.100377.
[10]	Huan, V. D.; Yamamura, S.; Ohtani, K.; Kasai, R.; Yamasaki, K.; Nham, N. T.; Chau, H. M. (1998). Oleanane Saponins from Polyscias fruticosa. Phytochemistry. 47, 451–457.
[11]	Castellano, José M., Sara Ramos-Romero, and Javier S. Perona. (2022). Oleanolic Acid: Extraction, Characterization and Biological Activity. Nutrients 14, no. 3: 623. https://doi.org/10.3390/nu14030623.
[12]	Thai. T. V., Hanh. T. T. H., Dat. N. T.. (2016). Validated High Performance Liquid Chromatography Method for Quantification of a Major Saponin in Polyscias fruticosa. Journal of Multidisciplinary Engineering Science and Technology (JMEST), 3, p. 4880-4882.
[13]	Kim. P. T. A., An. L. T. B., Chung. N. T., Truong. N. T., Thu L. T. A, Huan L. V. T. and Loc N. H. (2021). Growth and Oleanolic Acid Accumulation of Polyscias fruticosa Cell Suspension Cultures. Current Pharmaceutical Biotechnology, vol 22, 000-000.
[14]	ICH Harmonised tripartite guideline. (2005). Validation of analytical procedures: text and methodology. p. 1-13.
[15]	Ludwig Huber (2007), Validation and Qualification in Analytical Laboratories, 2nd edition. p. 144-146.

Cite This Article

Plain Text BibTeX RIS

APA Style

Le Thi Minh Thu, Pham Hoang Anh. (2023). Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province. Advances in Applied Sciences, 8(3), 100-105. https://doi.org/10.11648/j.aas.20230803.16

Copy | Download

ACS Style

Le Thi Minh Thu; Pham Hoang Anh. Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province. Adv. Appl. Sci. 2023, 8(3), 100-105. doi: 10.11648/j.aas.20230803.16

Copy | Download

AMA Style

Le Thi Minh Thu, Pham Hoang Anh. Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province. Adv Appl Sci. 2023;8(3):100-105. doi: 10.11648/j.aas.20230803.16

Copy | Download

@article{10.11648/j.aas.20230803.16,
  author = {Le Thi Minh Thu and Pham Hoang Anh},
  title = {Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province},
  journal = {Advances in Applied Sciences},
  volume = {8},
  number = {3},
  pages = {100-105},
  doi = {10.11648/j.aas.20230803.16},
  url = {https://doi.org/10.11648/j.aas.20230803.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aas.20230803.16},
  abstract = {Polyscias fruticosa (L.) Harms is a traditional medicine, which is planted widely in Tra Vinh province of Vietnam. It is used for nourishing the body, curing asthenia, emaciation, fatigue, poor digestion, poor metabolism, enhance working capacity and brain activity. Oleanolic acid (OA), a marker, is a sapogenin in radix polysciacis. Quantification of OA by high performance liquid chromatography (HPLC) makes an important contribution to the quality assessment of the medicinal plant of Polyscias fruticosa (L.) Harms. In order to develop and apply a method to evaluate the content OA in radix polysciacis, planted in Tra Vinh province, many conditions for extracting OA from radix polysciacis were investigated; many HPLC testing conditions were performed find a simple, accurate and fast analytical procedure for the quantification of OA in extract solution of radix polysciacis. As a result, the optimum extraction procedure of OA from the roots of Polyscias fruticosa (L.) Harms was found out; a simple, accurate and fast analytical procedure for the quantification of OA in radix polysciacis’s extract solution was developed, evaluated and applied to assets the OA content of 05 samples of radix polysciacis collected in different regions of Tra Vinh province. This developed method can control the quality of medicinal materials of Polyscias fruticosa (L.) Harms and its preparation effectively.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province
AU  - Le Thi Minh Thu
AU  - Pham Hoang Anh
Y1  - 2023/09/08
PY  - 2023
N1  - https://doi.org/10.11648/j.aas.20230803.16
DO  - 10.11648/j.aas.20230803.16
T2  - Advances in Applied Sciences
JF  - Advances in Applied Sciences
JO  - Advances in Applied Sciences
SP  - 100
EP  - 105
PB  - Science Publishing Group
SN  - 2575-1514
UR  - https://doi.org/10.11648/j.aas.20230803.16
AB  - Polyscias fruticosa (L.) Harms is a traditional medicine, which is planted widely in Tra Vinh province of Vietnam. It is used for nourishing the body, curing asthenia, emaciation, fatigue, poor digestion, poor metabolism, enhance working capacity and brain activity. Oleanolic acid (OA), a marker, is a sapogenin in radix polysciacis. Quantification of OA by high performance liquid chromatography (HPLC) makes an important contribution to the quality assessment of the medicinal plant of Polyscias fruticosa (L.) Harms. In order to develop and apply a method to evaluate the content OA in radix polysciacis, planted in Tra Vinh province, many conditions for extracting OA from radix polysciacis were investigated; many HPLC testing conditions were performed find a simple, accurate and fast analytical procedure for the quantification of OA in extract solution of radix polysciacis. As a result, the optimum extraction procedure of OA from the roots of Polyscias fruticosa (L.) Harms was found out; a simple, accurate and fast analytical procedure for the quantification of OA in radix polysciacis’s extract solution was developed, evaluated and applied to assets the OA content of 05 samples of radix polysciacis collected in different regions of Tra Vinh province. This developed method can control the quality of medicinal materials of Polyscias fruticosa (L.) Harms and its preparation effectively.
VL  - 8
IS  - 3
ER  -

Copy | Download

Author Information

Le Thi Minh Thu

Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City, Viet Nam
Pham Hoang Anh

Faculty of Pharmacy, Pham Ngoc Thanh University of Medicine, Ho Chi Minh City, Viet Nam

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Le Thi Minh Thu, Pham Hoang Anh. (2023). Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province. Advances in Applied Sciences, 8(3), 100-105. https://doi.org/10.11648/j.aas.20230803.16

Copy | Download

ACS Style

Le Thi Minh Thu; Pham Hoang Anh. Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province. Adv. Appl. Sci. 2023, 8(3), 100-105. doi: 10.11648/j.aas.20230803.16

Copy | Download

AMA Style

Le Thi Minh Thu, Pham Hoang Anh. Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province. Adv Appl Sci. 2023;8(3):100-105. doi: 10.11648/j.aas.20230803.16

Copy | Download

@article{10.11648/j.aas.20230803.16,
  author = {Le Thi Minh Thu and Pham Hoang Anh},
  title = {Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province},
  journal = {Advances in Applied Sciences},
  volume = {8},
  number = {3},
  pages = {100-105},
  doi = {10.11648/j.aas.20230803.16},
  url = {https://doi.org/10.11648/j.aas.20230803.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aas.20230803.16},
  abstract = {Polyscias fruticosa (L.) Harms is a traditional medicine, which is planted widely in Tra Vinh province of Vietnam. It is used for nourishing the body, curing asthenia, emaciation, fatigue, poor digestion, poor metabolism, enhance working capacity and brain activity. Oleanolic acid (OA), a marker, is a sapogenin in radix polysciacis. Quantification of OA by high performance liquid chromatography (HPLC) makes an important contribution to the quality assessment of the medicinal plant of Polyscias fruticosa (L.) Harms. In order to develop and apply a method to evaluate the content OA in radix polysciacis, planted in Tra Vinh province, many conditions for extracting OA from radix polysciacis were investigated; many HPLC testing conditions were performed find a simple, accurate and fast analytical procedure for the quantification of OA in extract solution of radix polysciacis. As a result, the optimum extraction procedure of OA from the roots of Polyscias fruticosa (L.) Harms was found out; a simple, accurate and fast analytical procedure for the quantification of OA in radix polysciacis’s extract solution was developed, evaluated and applied to assets the OA content of 05 samples of radix polysciacis collected in different regions of Tra Vinh province. This developed method can control the quality of medicinal materials of Polyscias fruticosa (L.) Harms and its preparation effectively.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province
AU  - Le Thi Minh Thu
AU  - Pham Hoang Anh
Y1  - 2023/09/08
PY  - 2023
N1  - https://doi.org/10.11648/j.aas.20230803.16
DO  - 10.11648/j.aas.20230803.16
T2  - Advances in Applied Sciences
JF  - Advances in Applied Sciences
JO  - Advances in Applied Sciences
SP  - 100
EP  - 105
PB  - Science Publishing Group
SN  - 2575-1514
UR  - https://doi.org/10.11648/j.aas.20230803.16
AB  - Polyscias fruticosa (L.) Harms is a traditional medicine, which is planted widely in Tra Vinh province of Vietnam. It is used for nourishing the body, curing asthenia, emaciation, fatigue, poor digestion, poor metabolism, enhance working capacity and brain activity. Oleanolic acid (OA), a marker, is a sapogenin in radix polysciacis. Quantification of OA by high performance liquid chromatography (HPLC) makes an important contribution to the quality assessment of the medicinal plant of Polyscias fruticosa (L.) Harms. In order to develop and apply a method to evaluate the content OA in radix polysciacis, planted in Tra Vinh province, many conditions for extracting OA from radix polysciacis were investigated; many HPLC testing conditions were performed find a simple, accurate and fast analytical procedure for the quantification of OA in extract solution of radix polysciacis. As a result, the optimum extraction procedure of OA from the roots of Polyscias fruticosa (L.) Harms was found out; a simple, accurate and fast analytical procedure for the quantification of OA in radix polysciacis’s extract solution was developed, evaluated and applied to assets the OA content of 05 samples of radix polysciacis collected in different regions of Tra Vinh province. This developed method can control the quality of medicinal materials of Polyscias fruticosa (L.) Harms and its preparation effectively.
VL  - 8
IS  - 3
ER  -

Copy | Download