Responses of the Whitefly <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) to Biologically Based Insecticides

Hail Kamel Shannag; Malak Saleh Al-Haj; John Lowell Capinera

doi:doi:10.11648/j.aje.20180203.12

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Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides

Hail Kamel Shannag, Malak Saleh Al-Haj, John Lowell Capinera

Published in American Journal of Entomology (Volume 2, Issue 3)

Received: 4 October 2018 Accepted: 19 October 2018 Published: 10 November 2018

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Abstract

The effects of three bio-insecticides Azatrol [neem: 1.2% azadirachtin A and B], Molt-X [neem: 3% azadirachtin], and Conserve SC [spinosad; 11.6% spinosyn A and spinosyn D], applied at different concentrations were evaluated on Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) under both laboratory and greenhouse conditions. Laboratory bioassays demonstrated that both neem-based insecticides were repellent to adult whiteflies in a dose-dependent manner. The amounts and frequency of honeydew excretion were significantly reduced up to 0.95 by foliar application of these insecticides at the labeled rate, as compared to untreated plants, with the neem products displaying greater effects on food uptake than spinosad. Reduced fecundity and egg hatch also were associated with these bio-insecticides. The bio-insecticides decreased significantly the survival of nymphs, egg hatch and adult emergence when applied systemically via the roots. However, the impacts of neem-based insecticides on all parameters tested were greater than that of spinosad. The results indicate that the biologically based formulations tested were effective in suppressing whitefly abundance and acting as an efficient repellent, though they were not able to completely inhibit food intake. The repellent and antifeedant activities of such natural products render plants unattractive to B. tabaci, thus potentially reducing the incidence of viral diseases transmitted by this pest. The systemic properties of these formulated biopesticides minimize their rapid degradation by strong ultraviolet light and their adverse effects on non-target organisms.

Published in	American Journal of Entomology (Volume 2, Issue 3)
DOI	10.11648/j.aje.20180203.12
Page(s)	28-35
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Whitefly, Bemisia tabaci, Biopesticides, Cucumber, Azadirachtin

References

[1]	Anonymous. (2010). National Agricultural Information System. http://nais-jordan. gov.jo /about.
[2]	Perring, T. M. (2001). The Bemisia tabaci species complex. Crop Protection, 20: 725-737.
[3]	Jones, D. R. (2003). Plant viruses transmitted by whiteflies. European Journal of Plant Pathology, 109: 195–219.
[4]	Cohen, S., Frank, E., Doyle, W. J., Skoner, D. P., Rabin, B. S. and Gwaltuey, J. M. Jr. (1998). Types of stressors that increase susceptibility to the common cold in healthy adults, Journal of Health Psychology. 17: 214-223.
[5]	Abou-Fakhr, H. E. M., Nemer, N. M., Hawi, Z. K. and Hanna, L. T. (2000). Responses of the sweetpotato whitefly, Bemisia tabaci, to the Chinaberry tree (Melia azedarach L.) and its extracts. Annals of Applied Biology, 137: 79-88.
[6]	Rapisarda, C. and Tropea Garzia, G. (2002). Tomato yellow leaf curl Sardinia virus and its vector Bemisia tabaci in Sicilia (Italy): present status and control possibilities. EPPO Bulletin, 32: 25-29.
[7]	Berlinger, M. J., 1986, Host plant resistance to Bemisia tabaci. Agriculture, Ecosystem and Environment, 17: 69-82.
[8]	Kaloshian, I. and Walling, L. L. (2005). Hemipterans as plant pathogens. Annual Review of Phytopathology, 43: 491-521.
[9]	Walling, L. L. (2008). Avoiding effective defenses: strategies employed by phloem-feeding insects, Plant Physiology, 146: 859-66.
[10]	Coudriet, D. L., Prabhaker, N., Kishaba, A. N. and Meyerdirk, D. E. (1985). Variation in developmental rate on different hosts and overwintering of the sweet potato whitefly, Bemisia tabaci (Homoptera: Aleyrodidae). Environmental Entomology, 14: 516-519.
[11]	Kim, H. G., Jeon, J. H., Kim, M. K. and Lee, H. S. (2005). Pharmacological effects of asaronaldehyde isolated from Acorus gramineus rhizome. Food Science and Biotechnology, 14: 685-688.
[12]	Rembold, H. (2002). Effect on viruses and organisms: Insect-growth and metamorphosis, in: Schmutterer, H. (Eds.), The Neem Tree, Vithalnagar, Juhu Schema, Mumbai, Neem foundation, pp. 237-254.
[13]	Cavalcante, G. M., Moreira, A. F. C. and Vasconcelos, S. D. (2006). Potencialidade inseticida de extratos aquosos de essências florestais sobre mosca-branca. Pesquisa Agropecuária Brasileira, 41: 9-14.
[14]	Kumar, P., Poehling, H-M. and Borgemeister, C. (2005). Effects of different application methods of neem against sweet potato whitefly Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) on tomato plants. Journal of Applied Entomology, 129: 489-497.
[15]	Kumar, P. and Poehling, H-M. (2006). Persistence of soil and foliar azadirachtin treatments to control sweet potato whitefly Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) on tomatoes under controlled (laboratory) and field (netted greenhouse) conditions in the humid tropics, Journal of Pest Sciences, 79: 189-199.
[16]	Kumar, P. and Poehling, H-M. (2007). Effects of azadirachtin, abamectin, and spinosad on sweetpotato whitefly (Homoptera: Aleyrodidae) on tomato plants under laboratory and greenhouse conditions in the humid tropics. Journal of Economic Entomology, 100: 411-420.
[17]	Souza, A. P. de. and Vendramim, J. D. (2005). Efeito translaminar, sistêmico e de contato de extrato aquoso de sementes de nim sobre Bemisia tabaci (Genn.) biótipo B em tomateiro. Neotropical Entomology, 34: 83-87.
[18]	Pearsall, I. A. and Hogue, E. J. (2000). Use of azadirachtin as a larvicide or feeding deterrent for control of western flower thrips in orchard systems. Phytoparasitica, 28: 219-228.
[19]	Gerald, W. (2001). Actions of insecticidal spinosyns on gama-aminobutyric acid responses for small-diameter cockroach neurons. Pesticide Biochemistry and Physiology, 71: 20-28.
[20]	Mark, H., Thompson, G. D., Subramanyam, B. and Athanassiou, C. G. (2011). Spinosad: A new natural product for stored grain protection. Journal of Stored Product Research, 47: 131-146.
[21]	SAS Institute. (2000). SAS statistics user’s manual, SAS version 9.2. NC: SAS Institute, Inc. Cary.
[22]	Püntener, W. (1981). Manual for Field trials in Plant Protection, Agric. Div, Ciba-Geigy Limited, Basle, Switzerland. p. 205.
[23]	McCloskey, C., Arnason, J. T., Donskov, N., Chenier, R., Kaminski, J. and Philogène, B. J. R. (1993). Third trophic level effects of azadirachtin, Canadian Entomologist, 125: 163-165.
[24]	Saxena, B. P., Tikku, K., Atal, C. K. and Koul, O. (1986). Insect antifertility and antifeedant allelochemics in Adhatoda vasica, Insect Science and its Application, 7: 489-493.
[25]	Saxena, R. C. and Khan, Z. R. (1988). New bioactive products: growth regulators, antifeedants, pheromones and other attractants, in: Marini-Bettòlo, G. B. (Eds.), Towards a Second Green Revolution: From Chemical to New Biological Technologies in Agriculture in the Tropics, Elsevier Science, pp. 303-317.
[26]	De Nardo, E. A. B., Costa, A. S. and Lourenção, A. L. (1997). Melia azedarach extract as an antifeedant to Bemisia tabaci (Homoptera: Aleyrodidae). Florida Entomologist, 80: 92-94.
[27]	Hilje, L., Stansly, P. A., Carballo, M. and Mora, G. A. (2003). Repellency and deterrency caused by plant extracts on Bemisia tabaci adults. Third International Bemisia Workshop, Barcelona, p. 103.
[28]	Wen, J-H., Hou, M-L., Lu, W. and Li, J-W. (2007). Effects of azadirachtin on host selection and oviposition of sweetpotato whitefly Bemisia tabaci Gennadius. Chinese Journal of Biological Control, 44: 491-496.
[29]	Wen, J-H., Lin, K-J., Hou, M-L., Lu, W. and Li, J-W. (2009). Influence of foliar and systemically applied azadirachtin on host-plant evaluation behaviour of the sweetpotato whitefly, Bemisia tabaci. Physiological Entomology, 34: 98-102.
[30]	Shannag, H. K., Capinera, J. L. and Freihat, N. M. (2013). Use of neem-based insecticides against southern armyworm, Spodoptera eridania (Stoll) (Lepidoptera: Noctuidae). Trends in Entomology, 9: 45-53.
[31]	Shannag, H. K., Capinera, J. L. and Freihat, N. M. (2014). Efficacy of different neem-based biopesticides against green peach aphid, Myzus persicae (Hemiptera: Aphididae). International Journal of Agricultural Policy and Research, 2: 061-068.
[32]	Lowery, D. T., Isman, M. B. and Brard, N. L. (1993). Laboratory and field evaluation of neem for the control of aphids (Homoptera: Aphididae). Journal of Economic Entomology, 86: 864-870.
[33]	Griffiths, D. C., Pickett, J. A., Smart, L. E. and Woodcock, C. M. (1989). Use of insect antifeedants against aphid vectors of plant virus disease. Journal of Pest Science, 27: 269-276.
[34]	Jenkins, D. A., Dunkel, F. V. and Gamby, K. T. (2003). Storage temperature of neem kernel extract: Differencial effects on oviposition deterrency and larval toxicity of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Environmental Entomolology, 32: 1283-1289.
[35]	Klocke, J. A., Balandrin, M. F., Barnby, M. A., Yanasaki, R. B., 1989, Limonoids, phenolics and furanocoumarins as insect antifeedants, repellents and growth inhibitory compounds, in: Arnason, J. T., Philogene, B. J. R., Morand, P. (Eds.) Insecticides of plant origin, ACS Symposium Series 387, pp. 136-149.
[36]	Barnby, M. A. and Klocke, J. A. (1987). Effects of azadirachtin on the nutrition and development of the tobacco budworm, Heliothis virescens (Fabr.) (Noctuidae). Journal of Insect Physiology, 33: 69-75.
[37]	Mordue, A. J. and Nisbet, A. J. (2000). Azadirachtin from the neem tree Azadirachta indica: its actions against insects. Anais da Sociedade Entomológica do Brasil, 29: 615-632.
[38]	Kleeberg, H. (1992). The NeemAzal conception test of systemic activity, in: Kleeberg, H. (Eds.), Proceedings of the 1st Workshop on Practice Oriented Results on Use and Production of Neem Ingredients, 19-20 June 1992, Druck and Graphic, Giessen, Germany Wetzlar, Germany, pp. 5-15.
[39]	Otto, D. (1994). Effects of the azadirachtin preparation “NeemAzal-W” on larvae and adults of Leptinotarsa decemlineata, in: Kleeberg, H. (Eds.), Practice Oriented Results on Use and Production of Neem-Ingredients and Pheromones, Druck und Graphic, Giessen, Germany, pp. 39-53.
[40]	Weintraub, P. G. and Horowitz, A. R. (1997). Systemic effect of a neem insecticide on Liriomyza huidobrensis. Phytoparasitica, 25: 283-289.
[41]	Prabhaker N, Toscano N. C. and Henneberry T. J. (1999). Comparison of neem, urea, and amitraz as oviposition suppressant and larvicides against Bemisia argentifolii (Homoptera: Aleyrodidae). Journal of Economic Entomology, 92: 40-46.
[42]	Thoeming, G., Borgemeister, C., Sétamou, M. and Poehling, H-M. (2003). Systemic effects of neem on western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae). Journal of Economic Entomology, 96: 817-825.
[43]	Ossiewatsch, H. R. (2000). Zur Wirkung von Samenkern-Wasserextrakten des Niembaumes Azadirachta indica (A Juss.) auf Blattlaeuse und ihre natuerlichen Gegenspieler. Ph. D. dissertation. Justus-Liebig University Giessen Germany.
[44]	Larew, H. G. (1988). Limited occurrence of foliar-, root- and seed-applied neem seed extract toxin in untreated plant parts. Journal of Economic Entomology, 81: 593-598.
[45]	Van Leeuwen, T., Van de Veire, M., Dermauw, W. and Tirry, L. (2006). Systemic toxicity of spinosad to the greenhouse whitefly Trialeurodes vaporariorum and to the cotton leaf worm Spodoptera littoralis. Phytoparasitica, 34: 102-108.
[46]	Van Leeuwen, T., Dermauw, W., Van De Veire, M. and Tirry, L. (2005). Systemic use of spinosad to control the two-spotted spider mite (Acari: Tetranychidae) on tomatoes grown in rockwool. Experimental and Applied Acarology, 37: 93-105.

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APA Style

Hail Kamel Shannag, Malak Saleh Al-Haj, John Lowell Capinera. (2018). Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides. American Journal of Entomology, 2(3), 28-35. https://doi.org/10.11648/j.aje.20180203.12

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ACS Style

Hail Kamel Shannag; Malak Saleh Al-Haj; John Lowell Capinera. Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides. Am. J. Entomol. 2018, 2(3), 28-35. doi: 10.11648/j.aje.20180203.12

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AMA Style

Hail Kamel Shannag, Malak Saleh Al-Haj, John Lowell Capinera. Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides. Am J Entomol. 2018;2(3):28-35. doi: 10.11648/j.aje.20180203.12

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@article{10.11648/j.aje.20180203.12,
  author = {Hail Kamel Shannag and Malak Saleh Al-Haj and John Lowell Capinera},
  title = {Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides},
  journal = {American Journal of Entomology},
  volume = {2},
  number = {3},
  pages = {28-35},
  doi = {10.11648/j.aje.20180203.12},
  url = {https://doi.org/10.11648/j.aje.20180203.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aje.20180203.12},
  abstract = {The effects of three bio-insecticides Azatrol [neem: 1.2% azadirachtin A and B], Molt-X [neem: 3% azadirachtin], and Conserve SC [spinosad; 11.6% spinosyn A and spinosyn D], applied at different concentrations were evaluated on Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) under both laboratory and greenhouse conditions. Laboratory bioassays demonstrated that both neem-based insecticides were repellent to adult whiteflies in a dose-dependent manner. The amounts and frequency of honeydew excretion were significantly reduced up to 0.95 by foliar application of these insecticides at the labeled rate, as compared to untreated plants, with the neem products displaying greater effects on food uptake than spinosad. Reduced fecundity and egg hatch also were associated with these bio-insecticides. The bio-insecticides decreased significantly the survival of nymphs, egg hatch and adult emergence when applied systemically via the roots. However, the impacts of neem-based insecticides on all parameters tested were greater than that of spinosad. The results indicate that the biologically based formulations tested were effective in suppressing whitefly abundance and acting as an efficient repellent, though they were not able to completely inhibit food intake. The repellent and antifeedant activities of such natural products render plants unattractive to B. tabaci, thus potentially reducing the incidence of viral diseases transmitted by this pest. The systemic properties of these formulated biopesticides minimize their rapid degradation by strong ultraviolet light and their adverse effects on non-target organisms.},
 year = {2018}
}

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TY  - JOUR
T1  - Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides
AU  - Hail Kamel Shannag
AU  - Malak Saleh Al-Haj
AU  - John Lowell Capinera
Y1  - 2018/11/10
PY  - 2018
N1  - https://doi.org/10.11648/j.aje.20180203.12
DO  - 10.11648/j.aje.20180203.12
T2  - American Journal of Entomology
JF  - American Journal of Entomology
JO  - American Journal of Entomology
SP  - 28
EP  - 35
PB  - Science Publishing Group
SN  - 2640-0537
UR  - https://doi.org/10.11648/j.aje.20180203.12
AB  - The effects of three bio-insecticides Azatrol [neem: 1.2% azadirachtin A and B], Molt-X [neem: 3% azadirachtin], and Conserve SC [spinosad; 11.6% spinosyn A and spinosyn D], applied at different concentrations were evaluated on Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) under both laboratory and greenhouse conditions. Laboratory bioassays demonstrated that both neem-based insecticides were repellent to adult whiteflies in a dose-dependent manner. The amounts and frequency of honeydew excretion were significantly reduced up to 0.95 by foliar application of these insecticides at the labeled rate, as compared to untreated plants, with the neem products displaying greater effects on food uptake than spinosad. Reduced fecundity and egg hatch also were associated with these bio-insecticides. The bio-insecticides decreased significantly the survival of nymphs, egg hatch and adult emergence when applied systemically via the roots. However, the impacts of neem-based insecticides on all parameters tested were greater than that of spinosad. The results indicate that the biologically based formulations tested were effective in suppressing whitefly abundance and acting as an efficient repellent, though they were not able to completely inhibit food intake. The repellent and antifeedant activities of such natural products render plants unattractive to B. tabaci, thus potentially reducing the incidence of viral diseases transmitted by this pest. The systemic properties of these formulated biopesticides minimize their rapid degradation by strong ultraviolet light and their adverse effects on non-target organisms.
VL  - 2
IS  - 3
ER  -

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Author Information

Hail Kamel Shannag

Faculty of Agriculture, Jordan University of Science and Technology, Irbid, Jordan
Malak Saleh Al-Haj

Faculty of Agriculture, Jordan University of Science and Technology, Irbid, Jordan
John Lowell Capinera

Institute of Food and Agricultural Sciences, University of Florida, Gainesville, United States

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Hail Kamel Shannag, Malak Saleh Al-Haj, John Lowell Capinera. (2018). Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides. American Journal of Entomology, 2(3), 28-35. https://doi.org/10.11648/j.aje.20180203.12

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ACS Style

Hail Kamel Shannag; Malak Saleh Al-Haj; John Lowell Capinera. Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides. Am. J. Entomol. 2018, 2(3), 28-35. doi: 10.11648/j.aje.20180203.12

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AMA Style

Hail Kamel Shannag, Malak Saleh Al-Haj, John Lowell Capinera. Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides. Am J Entomol. 2018;2(3):28-35. doi: 10.11648/j.aje.20180203.12

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@article{10.11648/j.aje.20180203.12,
  author = {Hail Kamel Shannag and Malak Saleh Al-Haj and John Lowell Capinera},
  title = {Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides},
  journal = {American Journal of Entomology},
  volume = {2},
  number = {3},
  pages = {28-35},
  doi = {10.11648/j.aje.20180203.12},
  url = {https://doi.org/10.11648/j.aje.20180203.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aje.20180203.12},
  abstract = {The effects of three bio-insecticides Azatrol [neem: 1.2% azadirachtin A and B], Molt-X [neem: 3% azadirachtin], and Conserve SC [spinosad; 11.6% spinosyn A and spinosyn D], applied at different concentrations were evaluated on Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) under both laboratory and greenhouse conditions. Laboratory bioassays demonstrated that both neem-based insecticides were repellent to adult whiteflies in a dose-dependent manner. The amounts and frequency of honeydew excretion were significantly reduced up to 0.95 by foliar application of these insecticides at the labeled rate, as compared to untreated plants, with the neem products displaying greater effects on food uptake than spinosad. Reduced fecundity and egg hatch also were associated with these bio-insecticides. The bio-insecticides decreased significantly the survival of nymphs, egg hatch and adult emergence when applied systemically via the roots. However, the impacts of neem-based insecticides on all parameters tested were greater than that of spinosad. The results indicate that the biologically based formulations tested were effective in suppressing whitefly abundance and acting as an efficient repellent, though they were not able to completely inhibit food intake. The repellent and antifeedant activities of such natural products render plants unattractive to B. tabaci, thus potentially reducing the incidence of viral diseases transmitted by this pest. The systemic properties of these formulated biopesticides minimize their rapid degradation by strong ultraviolet light and their adverse effects on non-target organisms.},
 year = {2018}
}

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TY  - JOUR
T1  - Responses of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) to Biologically Based Insecticides
AU  - Hail Kamel Shannag
AU  - Malak Saleh Al-Haj
AU  - John Lowell Capinera
Y1  - 2018/11/10
PY  - 2018
N1  - https://doi.org/10.11648/j.aje.20180203.12
DO  - 10.11648/j.aje.20180203.12
T2  - American Journal of Entomology
JF  - American Journal of Entomology
JO  - American Journal of Entomology
SP  - 28
EP  - 35
PB  - Science Publishing Group
SN  - 2640-0537
UR  - https://doi.org/10.11648/j.aje.20180203.12
AB  - The effects of three bio-insecticides Azatrol [neem: 1.2% azadirachtin A and B], Molt-X [neem: 3% azadirachtin], and Conserve SC [spinosad; 11.6% spinosyn A and spinosyn D], applied at different concentrations were evaluated on Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) under both laboratory and greenhouse conditions. Laboratory bioassays demonstrated that both neem-based insecticides were repellent to adult whiteflies in a dose-dependent manner. The amounts and frequency of honeydew excretion were significantly reduced up to 0.95 by foliar application of these insecticides at the labeled rate, as compared to untreated plants, with the neem products displaying greater effects on food uptake than spinosad. Reduced fecundity and egg hatch also were associated with these bio-insecticides. The bio-insecticides decreased significantly the survival of nymphs, egg hatch and adult emergence when applied systemically via the roots. However, the impacts of neem-based insecticides on all parameters tested were greater than that of spinosad. The results indicate that the biologically based formulations tested were effective in suppressing whitefly abundance and acting as an efficient repellent, though they were not able to completely inhibit food intake. The repellent and antifeedant activities of such natural products render plants unattractive to B. tabaci, thus potentially reducing the incidence of viral diseases transmitted by this pest. The systemic properties of these formulated biopesticides minimize their rapid degradation by strong ultraviolet light and their adverse effects on non-target organisms.
VL  - 2
IS  - 3
ER  -

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