American Journal of Entomology

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Nematicidal Activity of Aqueous Leaf Extracts of Datura metel, Datura innoxia and Brugmansia suaveolens

Received: May 11, 2017    Accepted: Aug. 03, 2017    Published: Nov. 06, 2017
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Abstract

The present study was aimed to screen the phytochemicals and to evaluate nematicidal activity of leaf of Datura metel, Datura innoxia and Brugmansia suaveolens. Phytoconstituents such as alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, cardiac glycosides, anthroquinone glycosides, saponins and triterpenes were analyzed by qualitatively in aqueous leaf extracts of Datura metel, Datura innoxia and Brugmansia suaveolens. The extracts were showed positive results for phytocompounds like alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, saponins and triterpenes in leaf of Datura metel, Datura innoxia and Brugmansia suaveolens. The cardiac glycosides and anthroquinone glycosides were absent in all three leaf extracts. The nematicidal potential of aqueous extracts of leaf of Datura metel, Datura innoxia and Brugmansia suaveolens against the most devastating root-knot nematode Meloidogyne incognita was studied. The leaf samples were subjected to nematicidal activity at different concentrations like 0.5%, 1%, 1.5% and 2%. The in vitro nematicidal activity showed that the aqueous leaf extract of Brugmansia suaveolens possessed maximum mortality on second stage juveniles of Meloidogyne incognita when compared with Datura metel and Datura innoxia. The concentrations of extract at 1.5% and 2% were found more effective against nematodes when compared to 0.5% and 1%. The mortality rate was also increased with increasing exposure time with leaf extracts. From these results, this study concluded that the nematicidal activity may be due to the presence of phytocompounds in leaf of Datura metel, Datura innoxia and Brugmansia suaveolens. This information would be useful for further characterization and purification of individual nematicidal compounds from these plants and it may also be helpful to find new bionematicidal compounds.

DOI 10.11648/j.aje.20170102.13
Published in American Journal of Entomology ( Volume 1, Issue 2, December 2017 )
Page(s) 39-45
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Datura metel, Datura innoxia, Brugmansia suaveolens, Phytochemicals, Meloidogyne incognita

References
[1] Mithraja M, J. Antonisamy, J. M. Mahesh, M. Paul, Z, M and Jeeva, S. (2011). Phytochemical studies on Azolla pinnata R. Br., Marsilea minuta L. and Salvinia molesta Mitch. Asian Pacific Journal of Tropical Biomedicine, S26–S29.
[2] Nasira, K. and Shahina, F. (2007). Nematode investigation in some cereals, fruits and vegetables of Pakistan. Pakistan Journal of Nematology, 24:1–7.
[3] Luc, M., Sikora, R. A. and Bridge, J. (2005). Plant parasitic nematodes in tropical and subtropical agriculture, 2nd edition. CAB International, Wallingford, Oxford, UK, 871.
[4] Khan, I. A. Sayed, M. Shaukat, S. S. and Handoo, Z. A. (2008). Efficacy of four plant extracts on nematodes associated with papaya in Sindh, Pakistan. Nematologia Mediterranea, 36:93–98.
[5] Javed, N. Gowen, S. R. Inam-ul-haq, M. Abdollah, K. and Shahina, F. (2006). Systemic and persistent effect of neem (Azadirachta indica) formulations against root-knot nematodes, Meloidogyne javanica and their storage life. Crop Protection, 26:911–916.
[6] Tsay, T. T. Wu, T. S. and Lin, Y. Y. (2004). Evaluation of Asteraceae plant for control of Meloidogyne incognita. Journal of Nematology, 36: 36–41.
[7] Hussain, M. A. Mukhtar, T. and Kayani, M. Z. (2011b). Efficacy evaluation of Azadirachta indica, Calotropis procera, Datura stramonium and Tagetes erecta against root-knot nematodes Meloidogyne incognita. Pakistan Journal of Botany, 43:197–204.
[8] Elbadri, G. A. Lee, D. W. Park, J. C. Yu, H. B. and Choo, H. Y. (2008). Evaluation of various plants extracts for their nematicidal efficacies against juveniles of Meloidogyne incognita. Journal of Asia-Pacific Entomology, 11:99–102.
[9] Isman, M. (2006). Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulates world. Annual Review of Entomology, 51:45–66.
[10] Amadioha, A. C. (2003). Evaluation of some plant leaf extracts against Colletotrichum lindemuthianum in cowpea. Acta phyto pathologicaet Entomologica Hungarica, 38 (3-4): 259–265.
[11] Agbenin O. N. (2004). Potentials of organic amendments in the control of plant parasitic nematodes. Plant Protection Science, 40:21–25.
[12] Dawar, S. Sattar, A. and Zaki, M. J. (2008). Seed dressing with biocontrol agents and nematicides for the control of root-knot nematode on sunflower and okra. Pakistan Journal of Botany, 40 (6):2683–2691.
[13] Adegbite, A. A. (2003). Comparative effects of Carbofuran and water extract of Chromolaena odorata on growth, yield and food components of root-knot nematode-infested soybean (Glycine max (L.) Merrill). Ph.D. thesis, University of Ibadan, Ibadan, Nigeria.
[14] Riga, E. Hooper, C. and Potter, J. (2005). In vitro effect of marigold seed exudates on plant parasitic nematodes. Phytoprotection, 86 (1): 31–35.
[15] Radwan, M. A. Abu-Elamayem, M. M. Kassem, S. M. and El-Maadawy, E. K. (2006). Soil amendment with dried weed leaves as non-chemical approach for the management of Meloidogyne incognita infecting tomato. Journal Communications in Agricultural and Applied Biological Sciences, 71(4):25–32.
[16] Kimpinski, J. Arsenault, W. J. Gallant, C. E. and Sanderson, J. B. (2000). The effect of marigolds (Tagetes spp.) and other cover crops on Pratylenchus penetrans and on following potato crops. Journal of Nematology, 32 (4):531–536.
[17] El-Hamawi, M. H. Youssef, M. M. A. Zawam, H. S. (2004). Management of Meloidogyne incognita, the root-knot nematode, on soybean as affected by marigold and Aambrosia maritime (damsisa) plants. Journal of Pest Science, 7 (2):95–98.
[18] Verma, K. K. (2006). Management of root-knot nematode (Meloidogyne javanica) in field pea (Pisum sativum) by intercropping with marigold (Tagetes erecta). Annals of Agri Bio Research, 11 (2): 121–122.
[19] Khalil, A. E. Shawky, and Samaa M. (2008). Combination of the nemato phagous fungus Paecilomyces lilacinus and aqueous leaf extracts in controlling Meloidogyne javanica infecting potato. Egyptian Journal of Agro Nematology, 6 (2):185–196.
[20] Saravanapriya, B. Sivakumar, M. Rajendran, G. and Kuttalam, S. (2004). Effect of different plant products on the hatching of Meloidogyne incognita eggs. Indian Journal of Nematology, 3: 690–697.
[21] Shawky, Samaa, M. Khalil, A. E. Soliman. and Manal, M. (2010). Non chemical control of root-knot nematode; Meloidogyne javanica on peanut in Egypt. Zagazig Journal of Agricultural Research, 37(1): 185–206.
[22] Rather, M. A. F. Ahmad and Siddiqui, M. A. (2007). Bio-efficacy of some botanical extracts for the management of root-knot nematode Meloidogyne incognita in Lycopersicon esculentum. National Journal of Life Sciences, 4:101–104.
[23] Shakeel, M. A. (2000). Control of citrus nematode (Tylenchulus semipenetrans) by the extracts of different plants (Neem, Datura, Ak) and their effect on plant growth variables. M. Sc. (Hons.) Thesis, University of Agriculture, Faisalabad.
[24] Ahmad, R. Shagab, M. N. Inam-ul-Hag, M. Javed, N. Dogar, M. A and Khan M. Y. (1996). Effect of soil amendment with Calotropis procera for the control of Meloidogyne javanica infection on eggplant. Pakistan Journal of Nematology, 14:55–59.
[25] Gemeinholzer, B. and Wink, M. (2001). Solanaceae: Occurrence of secondary compounds versus molecular phylogeny. In Solanaceae V: Advances in Taxonomy and Utilization. (van den Berg, R. G., Barendse, G. W. M., van der Weerden G. M. and Mariani, C. eds.). Nijmegen University Press, 165–178.
[26] Mann, J. (1996). Murder, magic and medicine. Oxford University Press Oxford, 82–84.
[27] Ogu, G. I. Williams, T. O. Nwachukwu, P. U. and Igere, B, E. (2012). Antimicrobial and phytochemical evaluation of the leaf, stem bark and root extracts of Cyathula prostrata (L) blume against some human pathogens. Journal of Intercultural Ethnopharmacology, 1(1):35–43.
[28] Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa. 2nd Edition Spectrum Books Limited, Ibadan, Nigeria, 1–15.
[29] Trease, G. E. and Evans, W. C. (1989). A text book of Pharmacognosy (13th edition) Bacilluere Tinal Ltd, London.
[30] Harborne, J. (1973). Phytochemical methods. Chapman and Hall, Ltd London, 49–88.
[31] Atta-ur-Rahman, A. M. Khan, M. Shabbir, M. Abid, M. I. Chaudhary, A. Nasreen, M. A. Maqbool, M. Shameel and R. Sualeh. (1997). Nematicidal activity of marine organisms. Pakistan Journal of Nematology, 15:95–100.
[32] Petti, S. and Scully, C. (2009). Polyphenols, oral health and disease: a review. Journal of Dentistry, 7 (6):413–423.
[33] Lata, N. and Dubey V. (2010). Preliminary phytochemical screening of Eichhornia crassipes: the world’s worst aquatic Weed. Journal of Pharmacy Research, 3:1240–1242.
[34] Capasso, A, De Feo, V. De Simone, F. and Sorrentino, L. (1997). Activity directed isolation of Spasmolytic (anti-cholinergic) alkaloids from Brugmansia arborea (L.) Lagerheim. International Journal of Pharmacognosy, 35(1):43–48.
[35] Capasso A. and De Feo, V. (2003). Alkaloids from Brugmansia arborea (L.) Lagerhein reduce morphine withdrawal in vitro. Phytotherapy Research, 17 (7):826–829.
[36] Kaur, S. and Joshi, M. (2013). In vitro evaluation of antimicrobial activity and phytochemical analysis of Calotropis procera, Eichhornia crassipes and Datura innoxia leaves. Asian Journal of Pharmaceutical and Clinical Research, 6 (5): 25–28.
[37] Sharma, M. C. and Sharma, S. (2010). Phytochemical, Preliminary Pharmacognostical and Antimicrobial Evaluation of Combined Crude Aqueous Extract. International Journal of Microbiological Research, 1(3): 166–170.
[38] Nandal, S. N. and Bhatti, D. S. (1986). The effect of certain edaphic factors on the nematicidal activity of plant extracts. Nematologia Mediterranea, 14:295–298.
[39] Sharma, N. and Trivedi, P. C. (2002). Screening of leaf extracts of some plants for their nematicidal and fungicidal properties against Meloidogyne incognita and Fusarium oxysporum. Asian Journal of Experimental Science, 16:21–28.
[40] [40] Haseeb, A. and Butool, F. (1996). Evaluation of nematicidal properties of some members of the family solanaceae. Bioresource Technology, 57:95–97.
[41] Akhtar, M. and Malik, A. (2000). Roles of organic soil amendments and soil organisms in the biological control of plant parasitic nematodes. Bioresource Technology, 74:35–47.
[42] Chitwood, D. J. (2002). Phytochemical based strategies for nematode control. Annual Review of Phytopathology, 40:221–249.
[43] Adegbite, A. A. (2011). Effects of some indigenous plant extracts as inhibitors of egg hatch in root-knot nematode (Meloidogyne incognita race 2). American Journal of Experimental Agriculture, 1:96–100.
[44] Kayani, M. Z. Ghulam Sarwar and Muhammad, S. (2001). Control of root-knot nematode (Meloidogyne incognita) on tomato plants by using root extracts of plants. Journal of Agriculture in the Tropics and Subtropics, 102 (2):143–146.
[45] Ahmad, A. and Alam, M. M. (1998). Effect of organic amendments alone and in combination with Paecilomyces lilacinus on Rotylenchulus reniformis attacking some economically important crop plants, Archives of Phytopathology and Plant Protection, 31(5):43–448.
[46] Ahmad, M. S. Mukhtar, T. and Ahmad, R. (2004). Some Studies on the control of Citrus Nematode (Tylenchulus semipenetrans) by Leaf Extracts of Three Plants and Their Effects on Plant Growth Variables. Asian Journal of Plant Sciences, 3 (5):544–548.
[47] Ayazpour, K. Hasanzadeh, H. and Arabzadegan, M. S. (2010). Evaluation of the control of citrus nematode (Tylenchulus semipenetrans) by leaf extracts of many plants and their effects on plant growth. African Journal of Agricultural Research, 5(14):1876–1880.
[48] Joymati, L. Sobita, N. Mohita and Dhanach and, C. H. (2003). Effect of leaf extracts of some medicinal plants on larval mortality of Meloidogyne incognita. Indian Journal of Nematology, 33:171–196.
[49] Vijayalakshmi, K. and Reshmi Basu, (1999). Seed coating of chickpea with neem based pesticidal formulations for the management of Meloidogyne incognita. Indian Journal of Nematology, 29: 28–32.
[50] Padhi, N. N. Gunanidhi, B. and Behera, G. (2000). Evaluation of nematicidal potential in ten indigenous plant species against Meloidogyne incognita. Indian Phytopathology, 53 (1):28–31.
[51] Reina, Y. Crozzoli, R. and Greco, N. (2002). Nematicidal effect of aqueous extracts from leaves of rubber tree (Calotropis procera) on different species of plant parasitic nematodes. Fitopatologia Venezolana, 15(2): 44–49.
[52] Nandakumar, A. Mayilvaganan, M. Sundararaju, P and Udayakumar, R. (2017). Phytochemical analysis and nematicidal activity of ethanolic leaf extracts of Datura metel, Datura innoxia and Brugmansia suaveolens against Meloidogyne incognita. Asian Journal of Biology. 2 (4): 1–11.
[53] Mohd Asif, Moh Tariq, Amir Khan, Mansoor and Siddiqui A. (2017). Biocidal and antinemic properties of aqueous extracts of Ageratum and Coccinia against root-knot nematode, Meloidogyne incognita in vitro. The Journal of Agricultural Sciences. Vol. 12 (2): 108–122.
[54] Nandi, B. (2016). Evaluation of nematicidal properties and inhibition of egg hatching activity of some medicinal plant extracts against Meloidogyne incognita. North Bengal University Journal of Animal Sciences 10: 89–94.
Cite This Article
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    Aiyadurai Nandakumar, Muthu Mayil Vaganan, Palaniyandi Sundararaju, Rajangam Udayakumar. (2017). Nematicidal Activity of Aqueous Leaf Extracts of Datura metel, Datura innoxia and Brugmansia suaveolens. American Journal of Entomology, 1(2), 39-45. https://doi.org/10.11648/j.aje.20170102.13

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    Aiyadurai Nandakumar; Muthu Mayil Vaganan; Palaniyandi Sundararaju; Rajangam Udayakumar. Nematicidal Activity of Aqueous Leaf Extracts of Datura metel, Datura innoxia and Brugmansia suaveolens. Am. J. Entomol. 2017, 1(2), 39-45. doi: 10.11648/j.aje.20170102.13

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    AMA Style

    Aiyadurai Nandakumar, Muthu Mayil Vaganan, Palaniyandi Sundararaju, Rajangam Udayakumar. Nematicidal Activity of Aqueous Leaf Extracts of Datura metel, Datura innoxia and Brugmansia suaveolens. Am J Entomol. 2017;1(2):39-45. doi: 10.11648/j.aje.20170102.13

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  • @article{10.11648/j.aje.20170102.13,
      author = {Aiyadurai Nandakumar and Muthu Mayil Vaganan and Palaniyandi Sundararaju and Rajangam Udayakumar},
      title = {Nematicidal Activity of Aqueous Leaf Extracts of Datura metel, Datura innoxia and Brugmansia suaveolens},
      journal = {American Journal of Entomology},
      volume = {1},
      number = {2},
      pages = {39-45},
      doi = {10.11648/j.aje.20170102.13},
      url = {https://doi.org/10.11648/j.aje.20170102.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.aje.20170102.13},
      abstract = {The present study was aimed to screen the phytochemicals and to evaluate nematicidal activity of leaf of Datura metel, Datura innoxia and Brugmansia suaveolens. Phytoconstituents such as alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, cardiac glycosides, anthroquinone glycosides, saponins and triterpenes were analyzed by qualitatively in aqueous leaf extracts of Datura metel, Datura innoxia and Brugmansia suaveolens. The extracts were showed positive results for phytocompounds like alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, saponins and triterpenes in leaf of Datura metel, Datura innoxia and Brugmansia suaveolens. The cardiac glycosides and anthroquinone glycosides were absent in all three leaf extracts. The nematicidal potential of aqueous extracts of leaf of Datura metel, Datura innoxia and Brugmansia suaveolens against the most devastating root-knot nematode Meloidogyne incognita was studied. The leaf samples were subjected to nematicidal activity at different concentrations like 0.5%, 1%, 1.5% and 2%. The in vitro nematicidal activity showed that the aqueous leaf extract of Brugmansia suaveolens possessed maximum mortality on second stage juveniles of Meloidogyne incognita when compared with Datura metel and Datura innoxia. The concentrations of extract at 1.5% and 2% were found more effective against nematodes when compared to 0.5% and 1%. The mortality rate was also increased with increasing exposure time with leaf extracts. From these results, this study concluded that the nematicidal activity may be due to the presence of phytocompounds in leaf of Datura metel, Datura innoxia and Brugmansia suaveolens. This information would be useful for further characterization and purification of individual nematicidal compounds from these plants and it may also be helpful to find new bionematicidal compounds.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Nematicidal Activity of Aqueous Leaf Extracts of Datura metel, Datura innoxia and Brugmansia suaveolens
    AU  - Aiyadurai Nandakumar
    AU  - Muthu Mayil Vaganan
    AU  - Palaniyandi Sundararaju
    AU  - Rajangam Udayakumar
    Y1  - 2017/11/06
    PY  - 2017
    N1  - https://doi.org/10.11648/j.aje.20170102.13
    DO  - 10.11648/j.aje.20170102.13
    T2  - American Journal of Entomology
    JF  - American Journal of Entomology
    JO  - American Journal of Entomology
    SP  - 39
    EP  - 45
    PB  - Science Publishing Group
    SN  - 2640-0537
    UR  - https://doi.org/10.11648/j.aje.20170102.13
    AB  - The present study was aimed to screen the phytochemicals and to evaluate nematicidal activity of leaf of Datura metel, Datura innoxia and Brugmansia suaveolens. Phytoconstituents such as alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, cardiac glycosides, anthroquinone glycosides, saponins and triterpenes were analyzed by qualitatively in aqueous leaf extracts of Datura metel, Datura innoxia and Brugmansia suaveolens. The extracts were showed positive results for phytocompounds like alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, saponins and triterpenes in leaf of Datura metel, Datura innoxia and Brugmansia suaveolens. The cardiac glycosides and anthroquinone glycosides were absent in all three leaf extracts. The nematicidal potential of aqueous extracts of leaf of Datura metel, Datura innoxia and Brugmansia suaveolens against the most devastating root-knot nematode Meloidogyne incognita was studied. The leaf samples were subjected to nematicidal activity at different concentrations like 0.5%, 1%, 1.5% and 2%. The in vitro nematicidal activity showed that the aqueous leaf extract of Brugmansia suaveolens possessed maximum mortality on second stage juveniles of Meloidogyne incognita when compared with Datura metel and Datura innoxia. The concentrations of extract at 1.5% and 2% were found more effective against nematodes when compared to 0.5% and 1%. The mortality rate was also increased with increasing exposure time with leaf extracts. From these results, this study concluded that the nematicidal activity may be due to the presence of phytocompounds in leaf of Datura metel, Datura innoxia and Brugmansia suaveolens. This information would be useful for further characterization and purification of individual nematicidal compounds from these plants and it may also be helpful to find new bionematicidal compounds.
    VL  - 1
    IS  - 2
    ER  - 

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Author Information
  • Post Graduate and Research Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam, Tamilnadu, India

  • ICAR-National Research Centre for Banana, Tiruchirappalli, Tamilnadu, India

  • ICAR-National Research Centre for Banana, Tiruchirappalli, Tamilnadu, India

  • Post Graduate and Research Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam, Tamilnadu, India

  • Section