Isolation, Identification and Characterization of a Lipase Producing <i>Pseudomonas</i>

Saadatullah; Ijaz Malook; Mehmood Jan; Waheedullah; Noor Muhammmad; Zia ur Rehman

doi:doi:10.11648/j.jb.20180202.15

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Isolation, Identification and Characterization of a Lipase Producing Pseudomonas

Saadatullah, Ijaz Malook, Mehmood Jan, Waheedullah, Noor Muhammmad, Zia ur Rehman

Published in Journal of Biomaterials (Volume 2, Issue 2)

Received: 20 October 2018 Accepted: 9 November 2018 Published: 17 December 2018

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Abstract

Lipases are hydrolytic enzymes and catalyze the hydrolysis of long-chain triacylglycerols into glycerol and fatty acid. Lipases are produced by plants, animals and microorganisms including bacteria and fungi. However microbial lipases, especially from bacteria, are more useful than their plant and animal derivatives because of several important properties. The primary goals of this research work is to isolate and identify a lipase producing Pseudomonas species from waste water samples collected from Dir lower, Peshawar and Kohat Khyber pakhtunkhwa Pakistan. The isolated bacteria were identified as Pseudomonas biochemically. The other purposes of this study are production, partial purification, characterization of lipase activity at different pH and incubation time, production and determination of molecular weight analysis. The lipase was partially purified up to 30% saturation using ammonium sulphate precipitation. Purity of lipases was cheeked by SDS-PAGE, showing the same banding pattern of all the lipases and the molecular weight were determined approximately 50kDA by comparing with protein marker bands. Spectrophotometric lipase assay was used for enzyme characterization. All the 7 isolates shows maximum activity at pH 7 after 48 hours of incubation and 37°C. Among all the isolates, isolate HSWPC shows highest activity of 110.11U /ml at pH 7 after 48 hours of incubation and 37°C. Pseudomonas lipases are widely used in food industry, detergent, paper, textile, leather and pharmaceutical industries because of their stability, selectivity and broad substrate specificity.

Published in	Journal of Biomaterials (Volume 2, Issue 2)
DOI	10.11648/j.jb.20180202.15
Page(s)	51-57
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Lipases, Pseudomonas, Production, Partial Purification, Molecular Characterization, Lipase Assay

References

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APA Style

Saadatullah, Ijaz Malook, Mehmood Jan, Waheedullah, Noor Muhammmad, et al. (2018). Isolation, Identification and Characterization of a Lipase Producing Pseudomonas. Journal of Biomaterials, 2(2), 51-57. https://doi.org/10.11648/j.jb.20180202.15

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ACS Style

Saadatullah; Ijaz Malook; Mehmood Jan; Waheedullah; Noor Muhammmad, et al. Isolation, Identification and Characterization of a Lipase Producing Pseudomonas. J. Biomater. 2018, 2(2), 51-57. doi: 10.11648/j.jb.20180202.15

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AMA Style

Saadatullah, Ijaz Malook, Mehmood Jan, Waheedullah, Noor Muhammmad, et al. Isolation, Identification and Characterization of a Lipase Producing Pseudomonas. J Biomater. 2018;2(2):51-57. doi: 10.11648/j.jb.20180202.15

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@article{10.11648/j.jb.20180202.15,
  author = {Saadatullah and Ijaz Malook and Mehmood Jan and Waheedullah and Noor Muhammmad and Zia ur Rehman},
  title = {Isolation, Identification and Characterization of a Lipase Producing Pseudomonas},
  journal = {Journal of Biomaterials},
  volume = {2},
  number = {2},
  pages = {51-57},
  doi = {10.11648/j.jb.20180202.15},
  url = {https://doi.org/10.11648/j.jb.20180202.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jb.20180202.15},
  abstract = {Lipases are hydrolytic enzymes and catalyze the hydrolysis of long-chain triacylglycerols into glycerol and fatty acid. Lipases are produced by plants, animals and microorganisms including bacteria and fungi. However microbial lipases, especially from bacteria, are more useful than their plant and animal derivatives because of several important properties. The primary goals of this research work is to isolate and identify a lipase producing Pseudomonas species from waste water samples collected from Dir lower, Peshawar and Kohat Khyber pakhtunkhwa Pakistan. The isolated bacteria were identified as Pseudomonas biochemically. The other purposes of this study are production, partial purification, characterization of lipase activity at different pH and incubation time, production and determination of molecular weight analysis. The lipase was partially purified up to 30% saturation using ammonium sulphate precipitation. Purity of lipases was cheeked by SDS-PAGE, showing the same banding pattern of all the lipases and the molecular weight were determined approximately 50kDA by comparing with protein marker bands. Spectrophotometric lipase assay was used for enzyme characterization. All the 7 isolates shows maximum activity at pH 7 after 48 hours of incubation and 37°C. Among all the isolates, isolate HSWPC shows highest activity of 110.11U /ml at pH 7 after 48 hours of incubation and 37°C. Pseudomonas lipases are widely used in food industry, detergent, paper, textile, leather and pharmaceutical industries because of their stability, selectivity and broad substrate specificity.},
 year = {2018}
}

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TY - JOUR
T1 - Isolation, Identification and Characterization of a Lipase Producing Pseudomonas
AU - Saadatullah
AU - Ijaz Malook
AU - Mehmood Jan
AU - Waheedullah
AU - Noor Muhammmad
AU - Zia ur Rehman
Y1 - 2018/12/17
PY - 2018
N1 - https://doi.org/10.11648/j.jb.20180202.15
DO - 10.11648/j.jb.20180202.15
T2 - Journal of Biomaterials
JF - Journal of Biomaterials
JO - Journal of Biomaterials
SP - 51
EP - 57
PB - Science Publishing Group
SN - 2640-2629
UR - https://doi.org/10.11648/j.jb.20180202.15
AB - Lipases are hydrolytic enzymes and catalyze the hydrolysis of long-chain triacylglycerols into glycerol and fatty acid. Lipases are produced by plants, animals and microorganisms including bacteria and fungi. However microbial lipases, especially from bacteria, are more useful than their plant and animal derivatives because of several important properties. The primary goals of this research work is to isolate and identify a lipase producing Pseudomonas species from waste water samples collected from Dir lower, Peshawar and Kohat Khyber pakhtunkhwa Pakistan. The isolated bacteria were identified as Pseudomonas biochemically. The other purposes of this study are production, partial purification, characterization of lipase activity at different pH and incubation time, production and determination of molecular weight analysis. The lipase was partially purified up to 30% saturation using ammonium sulphate precipitation. Purity of lipases was cheeked by SDS-PAGE, showing the same banding pattern of all the lipases and the molecular weight were determined approximately 50kDA by comparing with protein marker bands. Spectrophotometric lipase assay was used for enzyme characterization. All the 7 isolates shows maximum activity at pH 7 after 48 hours of incubation and 37°C. Among all the isolates, isolate HSWPC shows highest activity of 110.11U /ml at pH 7 after 48 hours of incubation and 37°C. Pseudomonas lipases are widely used in food industry, detergent, paper, textile, leather and pharmaceutical industries because of their stability, selectivity and broad substrate specificity.
VL - 2
IS - 2
ER -

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Author Information

Saadatullah

Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan
Ijaz Malook

Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan
Mehmood Jan

Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan
Waheedullah

Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan
Noor Muhammmad

Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan
Zia ur Rehman

Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan

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APA Style

Saadatullah, Ijaz Malook, Mehmood Jan, Waheedullah, Noor Muhammmad, et al. (2018). Isolation, Identification and Characterization of a Lipase Producing Pseudomonas. Journal of Biomaterials, 2(2), 51-57. https://doi.org/10.11648/j.jb.20180202.15

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ACS Style

Saadatullah; Ijaz Malook; Mehmood Jan; Waheedullah; Noor Muhammmad, et al. Isolation, Identification and Characterization of a Lipase Producing Pseudomonas. J. Biomater. 2018, 2(2), 51-57. doi: 10.11648/j.jb.20180202.15

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AMA Style

Saadatullah, Ijaz Malook, Mehmood Jan, Waheedullah, Noor Muhammmad, et al. Isolation, Identification and Characterization of a Lipase Producing Pseudomonas. J Biomater. 2018;2(2):51-57. doi: 10.11648/j.jb.20180202.15

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@article{10.11648/j.jb.20180202.15,
  author = {Saadatullah and Ijaz Malook and Mehmood Jan and Waheedullah and Noor Muhammmad and Zia ur Rehman},
  title = {Isolation, Identification and Characterization of a Lipase Producing Pseudomonas},
  journal = {Journal of Biomaterials},
  volume = {2},
  number = {2},
  pages = {51-57},
  doi = {10.11648/j.jb.20180202.15},
  url = {https://doi.org/10.11648/j.jb.20180202.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jb.20180202.15},
  abstract = {Lipases are hydrolytic enzymes and catalyze the hydrolysis of long-chain triacylglycerols into glycerol and fatty acid. Lipases are produced by plants, animals and microorganisms including bacteria and fungi. However microbial lipases, especially from bacteria, are more useful than their plant and animal derivatives because of several important properties. The primary goals of this research work is to isolate and identify a lipase producing Pseudomonas species from waste water samples collected from Dir lower, Peshawar and Kohat Khyber pakhtunkhwa Pakistan. The isolated bacteria were identified as Pseudomonas biochemically. The other purposes of this study are production, partial purification, characterization of lipase activity at different pH and incubation time, production and determination of molecular weight analysis. The lipase was partially purified up to 30% saturation using ammonium sulphate precipitation. Purity of lipases was cheeked by SDS-PAGE, showing the same banding pattern of all the lipases and the molecular weight were determined approximately 50kDA by comparing with protein marker bands. Spectrophotometric lipase assay was used for enzyme characterization. All the 7 isolates shows maximum activity at pH 7 after 48 hours of incubation and 37°C. Among all the isolates, isolate HSWPC shows highest activity of 110.11U /ml at pH 7 after 48 hours of incubation and 37°C. Pseudomonas lipases are widely used in food industry, detergent, paper, textile, leather and pharmaceutical industries because of their stability, selectivity and broad substrate specificity.},
 year = {2018}
}

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