Synthesis and Bioactivity of Silver Nanoparticles Against Bacteria (E. coli and Enterococcus sp. ) Isolated from Kalamu River, Kinshasa City, Democratic Republic of the Congo
Frontiers in Environmental Microbiology
Volume 4, Issue 1, February 2018, Pages: 29-40
Received: Dec. 23, 2017; Accepted: Jan. 19, 2018; Published: Mar. 8, 2018
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Authors
Koto-te-Nyiwa Ngbolua, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo; Department of Environmental Sciences, University of Gbadolite, Nord-Ubangi, Democratic Republic of the Congo; Higher Pedagogical Institute of Abumombazi, Nord Ubangi, Democratic Republic of the Congo
Gédéon Ngiala Bongo, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Amogu Domondo, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Beaudrique Nsimba, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Jeff Iteku, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Emmanuel Lengbiye, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Colette Ashande, Higher Pedagogical Institute of Abumombazi, Nord Ubangi, Democratic Republic of the Congo
Tshiama Claudine, Teaching and Administration in Nursing Care, Higher Institute of Medical Techniques, Kinshasa, Democratic Republic of the Congo
Clément Inkoto, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Lufuluabo Lufuluabo, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Pitchouna Kilunga, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Goslin Gafuene, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Crispin Mulaji, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
Théophile Mbemba, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
John Poté, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo; Department of F.-A. Forel for Environmental and Aquatic Sciences, University of Geneva, Geneva, Switzerland
Pius Mpiana, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo
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Abstract
The emergence of new infectious agents is a potential risk associated with genetic manipulation and field cultivation of genetically modified organisms and constitutes a new challenge in molecular epidemiology. The main objective of the current study was to synthesize silver nanoparticles and evaluate the antibacterial activity of these nanoparticles. E. coli and Enterococcus sp. were isolated from wastewater samples collected from Kalamu River. The preliminary characterization of silver nanoparticles was carried out using UV-visible spectrophotometer. Noble metals, such as silver nanoparticles, exhibit unique and adjustable optical properties due to their external plasmon resonance. The reduction of silver ions was monitored by measuring the UV-visible spectrum of the solutions after dilution of a small aliquot (0.2 mL) of the aqueous component. The antibiotic susceptibility test results confirmed the inactivity of these antibiotics tested against the wild strain of Enterococcus sp. The synthesized silver nanoparticles displayed a good antibacterial activity against Enterococcus sp. The synthesis of silver nanoparticles is designed precisely to alleviate this situation; and these results provide a strong evidence that silver nanoparticles can be used to fight antibiotic-resistant bacteria.
Keywords
Silver Nanoparticles, Antibacterial Activity, Antibiotic-Resistant Bacteria, Kalamu River, Annona senegalensis
To cite this article
Koto-te-Nyiwa Ngbolua, Gédéon Ngiala Bongo, Amogu Domondo, Beaudrique Nsimba, Jeff Iteku, Emmanuel Lengbiye, Colette Ashande, Tshiama Claudine, Clément Inkoto, Lufuluabo Lufuluabo, Pitchouna Kilunga, Goslin Gafuene, Crispin Mulaji, Théophile Mbemba, John Poté, Pius Mpiana, Synthesis and Bioactivity of Silver Nanoparticles Against Bacteria (E. coli and Enterococcus sp. ) Isolated from Kalamu River, Kinshasa City, Democratic Republic of the Congo, Frontiers in Environmental Microbiology. Vol. 4, No. 1, 2018, pp. 29-40. doi: 10.11648/j.fem.20180401.15
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Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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