Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains

Maryam A. S. Abubakr

doi:doi:10.11648/j.ijcda.20180401.14

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Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains

Maryam A. S. Abubakr

Published in International Journal of Clinical and Developmental Anatomy (Volume 4, Issue 1)

Received: 10 January 2018 Accepted: 1 February 2018 Published: 2 March 2018

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Abstract

This study aims to screen the antimicrobial activity of lactic acid bacteria (LAB) with probiotic properties isolated from human breast milk. A total of six from twenty five LAB isolated showed clear zone on modified MRS-CaCO3 agar, catalase negative and Gram positive were considered as LAB. All of the six selected isolates were able to tolerance pH 2, 0.3% bile salts for 3h. The antibacterial properties of these isolates against (Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Salmonella typhimurium) were examined using dual agar overlay and microtiter plates methods. Results found that both the cells and supernatants of six selected LAB isolated showed very good inhibitory activity against the target bacteria. The LAB-HM6 isolate showed the highest inhibitory activity (32.0 mm) against S. aureus followed by LAB-HM5 and LAB-HM4 (30.3 mm), then LAB-MH1 (29.3 mm) against S. typhimurium. Supernatant LAB-HM5 caused complete inhibition of all target bacteria, LAB-HM3 inhibited S. typhimurium and B. subtilis, and LAB-HM5 also inhibited the growth of S. aureus during 72 h incubation. Thus, these Lab isolates could be considered as potential antimicrobial probiotic strains human pathogens and should be further studied for their human health benefits.

Published in	International Journal of Clinical and Developmental Anatomy (Volume 4, Issue 1)
DOI	10.11648/j.ijcda.20180401.14
Page(s)	27-31
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Human Breast Milk, Lactic Acid Bacteria (LAB), Probiotic Properties, Antimicrobial Activity

References

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[2]	Aween, M., Hassan, Z. and Belal, M. (2012). Evaluation on Antibacterial Activity of Lactobacillus acidophilus Strains Isolated from Honey. American Journal of Applied Sciences, 9 (24): 807-817.
[3]	Azat, R., Liu, Y., Li, W., Kayir, A., Lin, D., Zhou, W. and Zheng, X. (2016). Probiotic Properties of Lactic Acid Bacteria Isolated from Traditionally Fermented Xinjiang cheese. J. Zhejiang. Univ-Sci B (Biomed & Biotegnol), 17 (8): 597-609.
[4]	Kanmani, P., Satish Kumar, R. and Yuvaraj, N. (2013). Probiotics and its Functionally valuable products a review. Crit. Rev. Food Sci. Nutr., 53 (6): 641-658.
[5]	Khaedr, E. E. (2006). Microbial and Processing Criteria for Production of Probiotics: A review. Food Technol. Biotechnol., 44: 371-379.
[6]	Mohammed, S. J. (2010). Therapeutic efficacy of Lactobacillus acidophilus against bacterial isolates from burn wounds. North American Journal of Medical Sciences, 2: 1947-2714.
[7]	Muhialdin, B. J., Hassan, Z., Sadon, S. K. Zulkifli, N. A. and Azfar, A. A. (2011a). Effect of pH and heat treatment on antifungal activity of Lactobacillus fermentum Te007, Lactobacillus pentosus G004 and Pediococcus pentosaceus Te010. Innovative Romanian Food Biotechnology, 8: 41-53.
[8]	Oliveira, R. B. P., Oliveira, A. L. and Glória, M. B. A. (2008). Screening of lactic acid bacteria from vacuum packaged beef for antimicrobial activity. Brazilian Journal of Microbiology, 39: 368-374.
[9]	Shehata, M. G., El-Sohaimy, S. A., El-Sahn, M. A. and Youssef, M. M. (2016). Screening of isolated Potential Probiotic Lactic Acid Bacteria for Cholesterol Lowering Property and Bile Salt Hydrolase Activity. Annals Agricultural Science, 61 (1): 65-75.
[10]	Shokryazdan, P., Sio, C., Kalavathy, R., Liang, J., Alitheen, N., Jahromy, M. and Ho, Y. (2014). Probiotic Potential of Lactobacillus Strains with Antimicrobial Activity Against Some Human Pathogenic Strains. BioMed Research International, 14: 1-16.
[11]	Turchi, B., Mancini, S. and Fratini, F. (2013). Preliminary Evaluation Probiotic Potential of Lactobacillus plantarum Strains Isolated from Italian Food Products. World J. Microbiol. Biotechnol., 29 (10): 1913-1922.

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APA Style

Maryam A. S. Abubakr. (2018). Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains. International Journal of Clinical and Developmental Anatomy, 4(1), 27-31. https://doi.org/10.11648/j.ijcda.20180401.14

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ACS Style

Maryam A. S. Abubakr. Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains. Int. J. Clin. Dev. Anat. 2018, 4(1), 27-31. doi: 10.11648/j.ijcda.20180401.14

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AMA Style

Maryam A. S. Abubakr. Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains. Int J Clin Dev Anat. 2018;4(1):27-31. doi: 10.11648/j.ijcda.20180401.14

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@article{10.11648/j.ijcda.20180401.14,
  author = {Maryam A. S. Abubakr},
  title = {Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {4},
  number = {1},
  pages = {27-31},
  doi = {10.11648/j.ijcda.20180401.14},
  url = {https://doi.org/10.11648/j.ijcda.20180401.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20180401.14},
  abstract = {This study aims to screen the antimicrobial activity of lactic acid bacteria (LAB) with probiotic properties isolated from human breast milk. A total of six from twenty five LAB isolated showed clear zone on modified MRS-CaCO3 agar, catalase negative and Gram positive were considered as LAB. All of the six selected isolates were able to tolerance pH 2, 0.3% bile salts for 3h. The antibacterial properties of these isolates against (Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Salmonella typhimurium) were examined using dual agar overlay and microtiter plates methods. Results found that both the cells and supernatants of six selected LAB isolated showed very good inhibitory activity against the target bacteria. The LAB-HM6 isolate showed the highest inhibitory activity (32.0 mm) against S. aureus followed by LAB-HM5 and LAB-HM4 (30.3 mm), then LAB-MH1 (29.3 mm) against S. typhimurium.  Supernatant LAB-HM5 caused complete inhibition of all target bacteria, LAB-HM3 inhibited S. typhimurium and B. subtilis, and LAB-HM5 also inhibited the growth of S. aureus during 72 h incubation. Thus, these Lab isolates could be considered as potential antimicrobial probiotic strains human pathogens and should be further studied for their human health benefits.},
 year = {2018}
}

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TY - JOUR
T1 - Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains
AU - Maryam A. S. Abubakr
Y1 - 2018/03/02
PY - 2018
N1 - https://doi.org/10.11648/j.ijcda.20180401.14
DO - 10.11648/j.ijcda.20180401.14
T2 - International Journal of Clinical and Developmental Anatomy
JF - International Journal of Clinical and Developmental Anatomy
JO - International Journal of Clinical and Developmental Anatomy
SP - 27
EP - 31
PB - Science Publishing Group
SN - 2469-8008
UR - https://doi.org/10.11648/j.ijcda.20180401.14
AB - This study aims to screen the antimicrobial activity of lactic acid bacteria (LAB) with probiotic properties isolated from human breast milk. A total of six from twenty five LAB isolated showed clear zone on modified MRS-CaCO3 agar, catalase negative and Gram positive were considered as LAB. All of the six selected isolates were able to tolerance pH 2, 0.3% bile salts for 3h. The antibacterial properties of these isolates against (Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Salmonella typhimurium) were examined using dual agar overlay and microtiter plates methods. Results found that both the cells and supernatants of six selected LAB isolated showed very good inhibitory activity against the target bacteria. The LAB-HM6 isolate showed the highest inhibitory activity (32.0 mm) against S. aureus followed by LAB-HM5 and LAB-HM4 (30.3 mm), then LAB-MH1 (29.3 mm) against S. typhimurium. Supernatant LAB-HM5 caused complete inhibition of all target bacteria, LAB-HM3 inhibited S. typhimurium and B. subtilis, and LAB-HM5 also inhibited the growth of S. aureus during 72 h incubation. Thus, these Lab isolates could be considered as potential antimicrobial probiotic strains human pathogens and should be further studied for their human health benefits.
VL - 4
IS - 1
ER -

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Author Information

Maryam A. S. Abubakr

Department Botany, Faculty of Science, Zawia University, Zawia, Libya

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APA Style

Maryam A. S. Abubakr. (2018). Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains. International Journal of Clinical and Developmental Anatomy, 4(1), 27-31. https://doi.org/10.11648/j.ijcda.20180401.14

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ACS Style

Maryam A. S. Abubakr. Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains. Int. J. Clin. Dev. Anat. 2018, 4(1), 27-31. doi: 10.11648/j.ijcda.20180401.14

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AMA Style

Maryam A. S. Abubakr. Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains. Int J Clin Dev Anat. 2018;4(1):27-31. doi: 10.11648/j.ijcda.20180401.14

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@article{10.11648/j.ijcda.20180401.14,
  author = {Maryam A. S. Abubakr},
  title = {Antimicrobial Activities of Lactic Acid Bacteria Strains Isolated from Human Breast Milk Against Human Pathogenic Strains},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {4},
  number = {1},
  pages = {27-31},
  doi = {10.11648/j.ijcda.20180401.14},
  url = {https://doi.org/10.11648/j.ijcda.20180401.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20180401.14},
  abstract = {This study aims to screen the antimicrobial activity of lactic acid bacteria (LAB) with probiotic properties isolated from human breast milk. A total of six from twenty five LAB isolated showed clear zone on modified MRS-CaCO3 agar, catalase negative and Gram positive were considered as LAB. All of the six selected isolates were able to tolerance pH 2, 0.3% bile salts for 3h. The antibacterial properties of these isolates against (Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Salmonella typhimurium) were examined using dual agar overlay and microtiter plates methods. Results found that both the cells and supernatants of six selected LAB isolated showed very good inhibitory activity against the target bacteria. The LAB-HM6 isolate showed the highest inhibitory activity (32.0 mm) against S. aureus followed by LAB-HM5 and LAB-HM4 (30.3 mm), then LAB-MH1 (29.3 mm) against S. typhimurium.  Supernatant LAB-HM5 caused complete inhibition of all target bacteria, LAB-HM3 inhibited S. typhimurium and B. subtilis, and LAB-HM5 also inhibited the growth of S. aureus during 72 h incubation. Thus, these Lab isolates could be considered as potential antimicrobial probiotic strains human pathogens and should be further studied for their human health benefits.},
 year = {2018}
}

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