Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of <i>Phyllanthus amarus</i> in Cadmium Induced Kidney Damage in Experimental Animals

Ogunnaike Philip Olubunmi; Olatunji Sunday Yinka; Owolabi Joshua Oladele; Olanrewaju Afees John; Baderinwa Deborah Boluwatife

doi:doi:10.11648/j.ijcda.20170304.12

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Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals

Ogunnaike Philip Olubunmi, Olatunji Sunday Yinka, Owolabi Joshua Oladele, Olanrewaju Afees John, Baderinwa Deborah Boluwatife

Received: 6 September 2017 Accepted: 28 September 2017 Published: 23 October 2017

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Abstract

Heavy metal poisoning is often as a result of prolonged occupational or domestic exposure to toxic metals and it is difficult to treat it immediately as the effects manifest over time. The use of herbal medicines and phytonutrients continues to expand rapidly across the world with many people now resorting to these products for treatment of various health challenges in different national healthcare settings. Chanca Piedra is a plant that has proven useful in assisting the treatment of various diseases. There have also been controversial claims on the effects of the plant on kidney stones and gall stones. Since cadmium is a very common nephrotoxic agent to which humans and animals are exposed frequently, this study has the potential to provide insight into the possible prophylactic and ameliorative effects of Chanca Piedra on kidney damage and it may also help in providing solutions to the problem of cadmium nephrotoxicity. The present study was carried out to investigate the prophylactic and ameliorative effects of orally administered ethanolic extract of Chanca Piedra (CP) (Phyllantus amarus) against nephrotoxicity produced by cadmium (40mg/kg BW) sulphate in adult male Wistar rats (Rattus novegicus). Forty-two (42) adult male Wistar rats were used in this study and were divided into seven (7) groups of (n=6) as follows: Group A (control), Group B (40mg/kg BW), Group C (100mg/kg BW of CP + 40mg/kg BW), Group D (200mg/kg BW of CP + 40mg/kg BW), Group E (40mg/kg BW + 100mg/kg BW of CP), Group F (40mg/kg BW + 200mg/kg BW of CP), Group G (200mg/kg BW of CP). Both cadmium and Chanca piedra were administered orally through oro-gastric cannula. The animals were sacrificed 24 hours after the last administration through cervical dislocation and blood samples were collected through cardiac puncture for analysis of superoxide dismutase and glutathione peroxidase and the kidney were fixed in 10% formal saline for 24hrs and then processed for histological analysis. Histological analysis using H&E stain for general histoarchitecture and Masson’s Trichrome for collagen fibres, indicated alterations in cell morphology of all the treated groups. The findings indicated that the administration of CP extract has no prophylactic or ameliorative effects on Cd-induced kidney damage and that CP had observable adverse effects on the kidneys of adult Wistar rats.

DOI	10.11648/j.ijcda.20170304.12
Published in	International Journal of Clinical and Developmental Anatomy (Volume 3, Issue 4, July 2017)
Page(s)	25-35
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Chanca Piedra, Phyllanthus amarus, Kidney Damage, Nephrotoxicity, Cadmium

References

[1]	Jarup L, Rogenfelt A, and Elinder C. (1983). biological half-time of cadmium in the blood of workers ater ceasation of exposure. Scandinavian journal of work, environment and Health, 9(4), 327-331.
[2]	Winston, David. (2011). Herbal and Nutritional Treatment of kidney stones. journal of the american herbalists guild, 10(2), 61.
[3]	Santos, A. R. S, Ailho, V. C, Yunes, R. A, Calixto, J. B. (1995). Analysis of the mechanism underlying the Anti-nociceptive Effect of the. General Pharmacol, 26, 1499-1506.
[4]	Shaw, D., Leon, C., Koleu, S., Murray, V. (1997). Traditional Remedies and Food Supplements. A Five- year Toxicology Study (1991,1995). Drug Safety, 17, 342-356.
[5]	Kaplowitz, N. (1997). Hepatotoxicity of Herbal Remedies. Insight into the intricacies of plant- animal warfare and Cell Death. Gastroenterology. 113, 1403-1412.
[6]	Krithika R., Verma R. J. (2009). Mitigation of carbon tetrachloride-induced damage by Phyllanthus amarus in liver of mice. Acta Pol, 66(4), 439-444.
[7]	Fernand V. (1998). Initial characterization of crude extracts from Phyllanthus amarus schum and thonn and Quassia amara L. using normal phase thin layer chromatography [Thesis]. Louisiana State University.
[8]	Naaz, F., Javed, S., Abdin, M. Z. (2007). Hepatoprotective effect of ethanolic extract of Phyllanthus amarus Schum. et Thonn. on. J Ethnopharmacol, 113, 503-509.
[9]	Matovi´c V, Buha A, Bulat Z, and Duki´c D. (2011). Cadmium toxicity revisited: focus on oxidative stress induction and interactions with zinc andmagnesium. Arhiv za Higijenu Radai Toksikologiju, 62(1), 65-76.
[10]	Patra R. C, Rautray A. K, and Swarup D. (2011). oxidative stress in lead and cadmium toxicity and its amelioration. Veterinary Medicine International.
[11]	Cuypers A, Plusquin M, Remans et al.,. (2010). Cadmium stress: an oxidative challenge. BioMetals, 23(5), 927-940.
[12]	Wang B, Shao C, Li Y, Tan Y, and Cai L,. (2012). cadmium and its epigenetic effects. Current Medicinal Chemistry, 19(16), 2611-2620.
[13]	Martinez-Zamudio. (2011). Environmental epigenetics in metal exposure. Epigenetics, 6(7), 820-827.
[14]	Luparello C, Sirchia R, and Longo A. (2011). Cadmium as atranscriptional modulator in human cells. critical reviews in toxicology, 41(1), 75-82.
[15]	Thevenod F. (2010). “Catch me if you can! Novel aspects of cadmium transport inmammalian cells”. biometals, 23(5), 857-875.
[16]	Van Kerkhove, Pennemans V, and Swennen Q. (2010). cadmium and transport of ions and substances across cellmembranes and epithelia. boimetals, 23(5), 823-855.
[17]	Wan L and Zhang H. (2012). Cadmium toxicity: effects on cytoskeleton, vesicular trafficking and cell well reconstruction. plant signalling and behaviour, 7(3), 345-348.
[18]	Vesey DA. (2010). Transport pathways for cadmium in the intestine and kidney proximal tubule: focus onthe interaction with essential metals. toxicology letters, 198(1), 13-19.
[19]	Abdulla M, Chmielnicka J. (1989). New aspects on the distribution and metabolism of essential trace elements after dietary exposure to toxic metals. Biological trace elements research, 23, 25-53.
[20]	Moulis J. (2010). Cellular Mechanisms of Cadmium toxicity related to the homeostasis of essential metals. biometals, 23(5), 877-896.
[21]	Shukla S and Singhal R. (1984). the present status of biological effects of toxic metals in the environment: lead, cadmium and manganese. canadian journal of physiology and pharmacology, 62(8), 1015-1031.
[22]	Schauder A, Avital A, Malik Z. (2010). regulation and gene expression of heme synthesis under heavy metal exposure- review. journal of environmental Pathology, Toxicology and oncology, 29(2), 137-158.
[23]	Cannino G, Ferruggia E, Luparello C and Rinaldi M. (2009). Cadmium and Mitochondria. 9(6), 377-384.
[24]	Arisawa K, Uemera H, Hiyoshi M, Dakeshita, Kitayama A, Saito H. (2007). cause specific mortality and cancer incidence rates in relation to urinary beta2-microglobulin: 23-year follow-up study in a cadmium-polluted area. toxicology letter, 28, 168-174.
[25]	World Health Organisation (WHO) (1992). Cadmium. Environmental Health Criteria 134.
[26]	Nordberg G. F, Nogawa K, Nordberg M and Friberg L. (2007). cadmium. In N. K. Nordberg G. F, Handbook of toxicology of metals (3 ed., pp. 445-486). Elsevier, Amsterdam, The Netherlands.
[27]	Jarup L, Berglund M, Elinder C. G, Nordberg G, Vahter M. (1998). Health effects of cadmium exposure: a review of literature and a risk estimate. Scand J Work Environ Health, 1(1), 51.
[28]	Bernard A, Lauwerys R. (1986). effects of cadmium exposure in humans. In F. E. C, & Berlin (Ed.), Handbook of expermental pharmacology (pp. 135-177). Springer-Verlag.
[29]	Prozialeck W. C, Edwards J. R, Woods J. M. (2006). the vascular endothelium as a target of cadmium toxicity. Life Sciences, 79(16), 1493-1506.
[30]	Asare G. A, Addo P, Bugyei K, Gyan B, Adjei S, Otu-Nyarko L. S, Wiredu E. K, Nyarko A (2011). Acute toxicity studies of aqueous leaf extract of Phyllanthus niruri. Interdiscip. Toxicol. 4: 206-210.
[31]	Mhaskar, K. S., Blatter, E. & Caius, J. F. (2000). Kirtikar and Basu’s Illustrated Indian Medicinal Plants, vol. 9. Sri Satguru Publications, Delhi, India, p.3074.
[32]	Raphael, K. R. & Kuttan, R. (2003). Inhibition of experimental gastric lesion and inflammation by Phyllanthus amarus extract. Journal of Ethnopharmacology, 87: 193–197.
[33]	Londhe, J. S., Devasagayam, T. P. A., Foo, L. Y. & Ghaskadbi, S. S. (2009). Radioprotective Properties of Polyphenols from Phyllanthus amarus Linn. Journal of radiation research, 50: 303-309.
[34]	Harikumar, K. B., Kuttan, G. & Kuttan, R. (2009). Inhibition of viral carcinogenesis by Phyllanthus amarus. Integrative Cancer Therapies, 8(3):254-60.
[35]	Rai, V., Khatoon, S., Rawat, A. K. S. & Mehrotra, S. (2007). Disruption of elements uptake due to excess chromium in indian medicinal plants. Biological Trace Element Research, 120: 127-132.
[36]	Ogunnaike Philip Olubunmi, Olatunji Sunday Yinka, Owolabi Joshua Oladele, Fabiyi Adetutu Olubusayo, Olanrewaju John Afees (2016). An Assessment of Renal Function Parameters on the Ameliorative Properties of Ginkgo Biloba Extract in Cadmium-Induced Nephrotoxicity in Adult Wistar Rats Model. American Journal of Clinical and Experimental Medicine. Vol. 4, No. 4, 2016, pp. 112-117. doi: 10.11648/j.ajcem.20160404.15.
[37]	Dudley R. E, Gammal L. M, Klaassen C. D (1985). Cadmium-induced hapatic and renal inury in chronically exposed rats: likely role of hepatic cadmium-metallothionein in nephrotoxicity. Toxicology and Applied Pharmacology 77 (3): 414-426.
[38]	Sudo J, Hayashi T, Kimura S, Kakuno K, Terui J, Takashima K, Soyama M. (1996). Mechanism of nephrotoxicity induced by repeated administration of Cadmium chloride in rats. Journal of Toxicology and Environmental Health 48 (4): 333348.
[39]	Thijssen S, Maringwa J, Faes C, Lambrichts I, Kerkhove E. V. (2007). Chronic exposure of mice to environmentally relevant, low doses of cadmium leads to early renal damage, not predicted by blood or urine cadmium levels. Toxicology 229 (2): 145-156.
[40]	Renugadevi J, Lamas M, Martin D, Islas S, Luna J (2012) Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicol 256: 128-134.
[41]	Tang W and Shaikh Z. A. (2001). Renal cortical mitochondrial dysfunction upon cadmium metallathionein administration to sprague-dawley rats. J Toxicol Environ Health – Part A. 63 (3): 221-235.
[42]	Foo L. Y and Wong H. (1992). phyllanthusin D, an unusual hydrolysable tannin from phyllanthus amarus. Phytochemistry, 31, 711-713.
[43]	Tagrajan, S. P., Jayaram, S. & Villicammai, T. (1990). Phyllanthus amarus and hepatitis-B. Lancet, 336: 949-950.
[44]	Oudhia, P. & Tripthi, R. S. (2002). Prospects of cultivation of medical plants in Chhattisgarh India. Recent progress in medical plants vol. 5 crop improvement production technology, trade and commerce. Sci. Thch. Pub. USA.; pp 211-236.
[45]	Manjrekar, A. P., Jisha, V., Bag, P. P., Adhikary, B., Pai, M. M., Hegde, A., et al., (2008). Effect of Phyllanthus niruri Linn treatment on liver, kidney and testes in CCl4 induced hepatotoxic rats. Indian J Exp Biol. 46: 514-520. 20.
[46]	Adedapo, A. A., Adegbayibi, A. Y., Emikpe, B. O. (2005). Some clinico-pathological changes associated with the aqueous extract of the leaves of Phyllanthus amarus in rats.
[47]	Varoni M. V, Palomba D, Gianorso S, Anania V. (2003). Cadmium as an environmental factor of hypertension in animals: new perspectives on mechanisms. Veterinary Research communications, 27(1), 807-810.
[48]	Atici, S., Cinel, I., Cinel, L., Doruk, N., Eskandari, G., Oral, U. (2005). Liver and kidney toxicity in chronic use of opioids: An experimental long term treatment model. J Biosci; 30: 245-252.
[49]	Galati, G., O'Brien, P. J. (2004). Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic Biol Med.; 37: 287-303.
[50]	Perry, L. M. (1980). Medicinal plants of East and South-East Asia, MIT Press, Cambridge Massachusetts.

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Ogunnaike Philip Olubunmi, Olatunji Sunday Yinka, Owolabi Joshua Oladele, Olanrewaju Afees John, Baderinwa Deborah Boluwatife. (2017). Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals. International Journal of Clinical and Developmental Anatomy, 3(4), 25-35. https://doi.org/10.11648/j.ijcda.20170304.12

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ACS Style

Ogunnaike Philip Olubunmi; Olatunji Sunday Yinka; Owolabi Joshua Oladele; Olanrewaju Afees John; Baderinwa Deborah Boluwatife. Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals. Int. J. Clin. Dev. Anat. 2017, 3(4), 25-35. doi: 10.11648/j.ijcda.20170304.12

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AMA Style

Ogunnaike Philip Olubunmi, Olatunji Sunday Yinka, Owolabi Joshua Oladele, Olanrewaju Afees John, Baderinwa Deborah Boluwatife. Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals. Int J Clin Dev Anat. 2017;3(4):25-35. doi: 10.11648/j.ijcda.20170304.12

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@article{10.11648/j.ijcda.20170304.12,
  author = {Ogunnaike Philip Olubunmi and Olatunji Sunday Yinka and Owolabi Joshua Oladele and Olanrewaju Afees John and Baderinwa Deborah Boluwatife},
  title = {Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {3},
  number = {4},
  pages = {25-35},
  doi = {10.11648/j.ijcda.20170304.12},
  url = {https://doi.org/10.11648/j.ijcda.20170304.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20170304.12},
  abstract = {Heavy metal poisoning is often as a result of prolonged occupational or domestic exposure to toxic metals and it is difficult to treat it immediately as the effects manifest over time. The use of herbal medicines and phytonutrients continues to expand rapidly across the world with many people now resorting to these products for treatment of various health challenges in different national healthcare settings. Chanca Piedra is a plant that has proven useful in assisting the treatment of various diseases. There have also been controversial claims on the effects of the plant on kidney stones and gall stones. Since cadmium is a very common nephrotoxic agent to which humans and animals are exposed frequently, this study has the potential to provide insight into the possible prophylactic and ameliorative effects of Chanca Piedra on kidney damage and it may also help in providing solutions to the problem of cadmium nephrotoxicity. The present study was carried out to investigate the prophylactic and ameliorative effects of orally administered ethanolic extract of Chanca Piedra (CP) (Phyllantus amarus) against nephrotoxicity produced by cadmium (40mg/kg BW) sulphate in adult male Wistar rats (Rattus novegicus). Forty-two (42) adult male Wistar rats were used in this study and were divided into seven (7) groups of (n=6) as follows: Group A (control), Group B (40mg/kg BW), Group C (100mg/kg BW of CP + 40mg/kg BW), Group D (200mg/kg BW of CP + 40mg/kg BW), Group E (40mg/kg BW + 100mg/kg BW of CP), Group F (40mg/kg BW + 200mg/kg BW of CP), Group G (200mg/kg BW of CP). Both cadmium and Chanca piedra were administered orally through oro-gastric cannula. The animals were sacrificed 24 hours after the last administration through cervical dislocation and blood samples were collected through cardiac puncture for analysis of superoxide dismutase and glutathione peroxidase and the kidney were fixed in 10% formal saline for 24hrs and then processed for histological analysis. Histological analysis using H&E stain for general histoarchitecture and Masson’s Trichrome for collagen fibres, indicated alterations in cell morphology of all the treated groups. The findings indicated that the administration of CP extract has no prophylactic or ameliorative effects on Cd-induced kidney damage and that CP had observable adverse effects on the kidneys of adult Wistar rats.},
 year = {2017}
}

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TY  - JOUR
T1  - Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals
AU  - Ogunnaike Philip Olubunmi
AU  - Olatunji Sunday Yinka
AU  - Owolabi Joshua Oladele
AU  - Olanrewaju Afees John
AU  - Baderinwa Deborah Boluwatife
Y1  - 2017/10/23
PY  - 2017
N1  - https://doi.org/10.11648/j.ijcda.20170304.12
DO  - 10.11648/j.ijcda.20170304.12
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 25
EP  - 35
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20170304.12
AB  - Heavy metal poisoning is often as a result of prolonged occupational or domestic exposure to toxic metals and it is difficult to treat it immediately as the effects manifest over time. The use of herbal medicines and phytonutrients continues to expand rapidly across the world with many people now resorting to these products for treatment of various health challenges in different national healthcare settings. Chanca Piedra is a plant that has proven useful in assisting the treatment of various diseases. There have also been controversial claims on the effects of the plant on kidney stones and gall stones. Since cadmium is a very common nephrotoxic agent to which humans and animals are exposed frequently, this study has the potential to provide insight into the possible prophylactic and ameliorative effects of Chanca Piedra on kidney damage and it may also help in providing solutions to the problem of cadmium nephrotoxicity. The present study was carried out to investigate the prophylactic and ameliorative effects of orally administered ethanolic extract of Chanca Piedra (CP) (Phyllantus amarus) against nephrotoxicity produced by cadmium (40mg/kg BW) sulphate in adult male Wistar rats (Rattus novegicus). Forty-two (42) adult male Wistar rats were used in this study and were divided into seven (7) groups of (n=6) as follows: Group A (control), Group B (40mg/kg BW), Group C (100mg/kg BW of CP + 40mg/kg BW), Group D (200mg/kg BW of CP + 40mg/kg BW), Group E (40mg/kg BW + 100mg/kg BW of CP), Group F (40mg/kg BW + 200mg/kg BW of CP), Group G (200mg/kg BW of CP). Both cadmium and Chanca piedra were administered orally through oro-gastric cannula. The animals were sacrificed 24 hours after the last administration through cervical dislocation and blood samples were collected through cardiac puncture for analysis of superoxide dismutase and glutathione peroxidase and the kidney were fixed in 10% formal saline for 24hrs and then processed for histological analysis. Histological analysis using H&E stain for general histoarchitecture and Masson’s Trichrome for collagen fibres, indicated alterations in cell morphology of all the treated groups. The findings indicated that the administration of CP extract has no prophylactic or ameliorative effects on Cd-induced kidney damage and that CP had observable adverse effects on the kidneys of adult Wistar rats.
VL  - 3
IS  - 4
ER  -

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Author Information

Ogunnaike Philip Olubunmi

Department of Anatomy, Ben Carson Senior School of Medicine, Babcock University, Ilisan Remo, Nigeria
Olatunji Sunday Yinka

Department of Anatomy, Ben Carson Senior School of Medicine, Babcock University, Ilisan Remo, Nigeria; Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Nigeria
Owolabi Joshua Oladele

Department of Anatomy, Ben Carson Senior School of Medicine, Babcock University, Ilisan Remo, Nigeria
Olanrewaju Afees John

Department of Anatomy, Ben Carson Senior School of Medicine, Babcock University, Ilisan Remo, Nigeria
Baderinwa Deborah Boluwatife

Department of Anatomy, Ben Carson Senior School of Medicine, Babcock University, Ilisan Remo, Nigeria

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Ogunnaike Philip Olubunmi, Olatunji Sunday Yinka, Owolabi Joshua Oladele, Olanrewaju Afees John, Baderinwa Deborah Boluwatife. (2017). Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals. International Journal of Clinical and Developmental Anatomy, 3(4), 25-35. https://doi.org/10.11648/j.ijcda.20170304.12

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ACS Style

Ogunnaike Philip Olubunmi; Olatunji Sunday Yinka; Owolabi Joshua Oladele; Olanrewaju Afees John; Baderinwa Deborah Boluwatife. Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals. Int. J. Clin. Dev. Anat. 2017, 3(4), 25-35. doi: 10.11648/j.ijcda.20170304.12

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AMA Style

Ogunnaike Philip Olubunmi, Olatunji Sunday Yinka, Owolabi Joshua Oladele, Olanrewaju Afees John, Baderinwa Deborah Boluwatife. Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals. Int J Clin Dev Anat. 2017;3(4):25-35. doi: 10.11648/j.ijcda.20170304.12

Copy | Download

@article{10.11648/j.ijcda.20170304.12,
  author = {Ogunnaike Philip Olubunmi and Olatunji Sunday Yinka and Owolabi Joshua Oladele and Olanrewaju Afees John and Baderinwa Deborah Boluwatife},
  title = {Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals},
  journal = {International Journal of Clinical and Developmental Anatomy},
  volume = {3},
  number = {4},
  pages = {25-35},
  doi = {10.11648/j.ijcda.20170304.12},
  url = {https://doi.org/10.11648/j.ijcda.20170304.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcda.20170304.12},
  abstract = {Heavy metal poisoning is often as a result of prolonged occupational or domestic exposure to toxic metals and it is difficult to treat it immediately as the effects manifest over time. The use of herbal medicines and phytonutrients continues to expand rapidly across the world with many people now resorting to these products for treatment of various health challenges in different national healthcare settings. Chanca Piedra is a plant that has proven useful in assisting the treatment of various diseases. There have also been controversial claims on the effects of the plant on kidney stones and gall stones. Since cadmium is a very common nephrotoxic agent to which humans and animals are exposed frequently, this study has the potential to provide insight into the possible prophylactic and ameliorative effects of Chanca Piedra on kidney damage and it may also help in providing solutions to the problem of cadmium nephrotoxicity. The present study was carried out to investigate the prophylactic and ameliorative effects of orally administered ethanolic extract of Chanca Piedra (CP) (Phyllantus amarus) against nephrotoxicity produced by cadmium (40mg/kg BW) sulphate in adult male Wistar rats (Rattus novegicus). Forty-two (42) adult male Wistar rats were used in this study and were divided into seven (7) groups of (n=6) as follows: Group A (control), Group B (40mg/kg BW), Group C (100mg/kg BW of CP + 40mg/kg BW), Group D (200mg/kg BW of CP + 40mg/kg BW), Group E (40mg/kg BW + 100mg/kg BW of CP), Group F (40mg/kg BW + 200mg/kg BW of CP), Group G (200mg/kg BW of CP). Both cadmium and Chanca piedra were administered orally through oro-gastric cannula. The animals were sacrificed 24 hours after the last administration through cervical dislocation and blood samples were collected through cardiac puncture for analysis of superoxide dismutase and glutathione peroxidase and the kidney were fixed in 10% formal saline for 24hrs and then processed for histological analysis. Histological analysis using H&E stain for general histoarchitecture and Masson’s Trichrome for collagen fibres, indicated alterations in cell morphology of all the treated groups. The findings indicated that the administration of CP extract has no prophylactic or ameliorative effects on Cd-induced kidney damage and that CP had observable adverse effects on the kidneys of adult Wistar rats.},
 year = {2017}
}

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TY  - JOUR
T1  - Histomorphological Changes in Kidney Associated with the Ethanolic Extracts of the Leaves of Phyllanthus amarus in Cadmium Induced Kidney Damage in Experimental Animals
AU  - Ogunnaike Philip Olubunmi
AU  - Olatunji Sunday Yinka
AU  - Owolabi Joshua Oladele
AU  - Olanrewaju Afees John
AU  - Baderinwa Deborah Boluwatife
Y1  - 2017/10/23
PY  - 2017
N1  - https://doi.org/10.11648/j.ijcda.20170304.12
DO  - 10.11648/j.ijcda.20170304.12
T2  - International Journal of Clinical and Developmental Anatomy
JF  - International Journal of Clinical and Developmental Anatomy
JO  - International Journal of Clinical and Developmental Anatomy
SP  - 25
EP  - 35
PB  - Science Publishing Group
SN  - 2469-8008
UR  - https://doi.org/10.11648/j.ijcda.20170304.12
AB  - Heavy metal poisoning is often as a result of prolonged occupational or domestic exposure to toxic metals and it is difficult to treat it immediately as the effects manifest over time. The use of herbal medicines and phytonutrients continues to expand rapidly across the world with many people now resorting to these products for treatment of various health challenges in different national healthcare settings. Chanca Piedra is a plant that has proven useful in assisting the treatment of various diseases. There have also been controversial claims on the effects of the plant on kidney stones and gall stones. Since cadmium is a very common nephrotoxic agent to which humans and animals are exposed frequently, this study has the potential to provide insight into the possible prophylactic and ameliorative effects of Chanca Piedra on kidney damage and it may also help in providing solutions to the problem of cadmium nephrotoxicity. The present study was carried out to investigate the prophylactic and ameliorative effects of orally administered ethanolic extract of Chanca Piedra (CP) (Phyllantus amarus) against nephrotoxicity produced by cadmium (40mg/kg BW) sulphate in adult male Wistar rats (Rattus novegicus). Forty-two (42) adult male Wistar rats were used in this study and were divided into seven (7) groups of (n=6) as follows: Group A (control), Group B (40mg/kg BW), Group C (100mg/kg BW of CP + 40mg/kg BW), Group D (200mg/kg BW of CP + 40mg/kg BW), Group E (40mg/kg BW + 100mg/kg BW of CP), Group F (40mg/kg BW + 200mg/kg BW of CP), Group G (200mg/kg BW of CP). Both cadmium and Chanca piedra were administered orally through oro-gastric cannula. The animals were sacrificed 24 hours after the last administration through cervical dislocation and blood samples were collected through cardiac puncture for analysis of superoxide dismutase and glutathione peroxidase and the kidney were fixed in 10% formal saline for 24hrs and then processed for histological analysis. Histological analysis using H&E stain for general histoarchitecture and Masson’s Trichrome for collagen fibres, indicated alterations in cell morphology of all the treated groups. The findings indicated that the administration of CP extract has no prophylactic or ameliorative effects on Cd-induced kidney damage and that CP had observable adverse effects on the kidneys of adult Wistar rats.
VL  - 3
IS  - 4
ER  -

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