Journal of Health and Environmental Research

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Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria

Received: Jan. 06, 2018    Accepted: Feb. 16, 2018    Published: Mar. 23, 2018
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Abstract

In recent times, surfaces of hospital environment, constantly exposed to activities of patients and health-care workers, have been identified as potential routes for horizontal dissemination of drug resistant microorganisms of public health significance. This study was conducted to investigate the antibiotic resistance profile of bacteria isolated from samples collected from door handles of various units in University of Benin Teaching Hospital (UBTH), Benin City, Edo State, Nigeria. Out of the 66 door handles sampled and analysed bacteriologically, 68 predominant bacteria distributed among five (5) species of Escherichia coli (30.9%), Staphylococcusaureus (26.5%), Pseudomonas aeruginosa (17.6%), Streptococcus pneumonia (13.2%) and Bacillus subtilis (11.8%) were isolated. Kirby Bauer techniques of disk diffusion antibiotic susceptibility test on the isolates using 9 commercial antibiotics according to Clinical Laboratory Standards Institute (CLSI) procedure showed resistance of P. aeruginosa to five (5) drugs (Sulfamethoxazole-Trimethoprim, Streptomycin, Ampicillin, Cephalexin and Nalidixic acid), E. coli to five (5) drugs (Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin, Cephalexin and Nalidixic acid), S. pneumoniaeto two drugs (Ampicilin and Nalidixic acid) and S aureus to only Nalidixic acid. Agarose gel electrophoresis carried out on their plasmid DNA revealed detectable fragments in P. aeruginosa and E. coli. After plasmid curing, the isolates became sensitive to Streptomycin, Cephalexin,Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin and Ciprofloxacin. Findings from this study suggest the presence of plasmid mediated multi-drug resistant P. aeruginosa and E. coli on surfaces of hospital door handles. Hence, public education on proper personal hygiene and disinfection techniques are recommended in hospitals.

DOI 10.11648/j.jher.20180401.15
Published in Journal of Health and Environmental Research ( Volume 4, Issue 1, March 2018 )
Page(s) 35-41
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antibiotics, Door Handles, Plasmid, Bacteria

References
[1] Weber D. J., Anderson, D., Rutala, W. A (2013). The role of the surface environment in healthcareassociated infections. Current Opinion 26 (4): 338-344.
[2] Klevens, R. M., Edwards, J. R., Richards, C. L., Horan, T. C., Gaynes, R. P., Pollack, D. A (2007). Estimating health care-associated infections and deaths in US hospitals, 2002. Public Health Reports 122: 160-166.
[3] Weinstein, R. A (1991). Epidemiology and control of nosocomial infections in adult intensive care units. American Journal of Medicine 199:179-184.
[4] Weber D. J., Rutala, W. A., Miller, M. B., Huslage, K, Sickbert-Bennett, E (1991). Role of hospital surfaces in the transmission of emerging health care associated pathogens: Norovirus, Clostridium difficile, and Acinetobacterspecies. American Journal Infection Control 38:25-33.
[5] Hota, B (2004). Contamination, disinfection, and cross-colonization: are hospital surfaces reservoirs for nosocomial infection? Clinical Infection Disease 39:1182-1189.
[6] Boyce, J (2007). Environmental contamination makes an important contribution to hospital infection. Journal of Hospital Infection 65: 50-54.
[7] Bakkali, M. E. L., Hmid, K., Kari, K. E., Zouhdi, M., Mzibri, M. E. L (2015). Characterization of Bacterial Strains and their Resistance Status in Hospital Environment. Journal of Tropical Diseases 4:180. doi:10.4172/2329-891X.1000180.
[8] Wang, H. P., Zhang, H. J, Liu, J., Dong Q., Duan S, Ge, J. Q., Wang, Z. H, Zhang, Z (2017). Antimicrobial resistance of 3 types of gram-negative bacteria isolated from hospital surfaces and the hands of health care workers. American Journal Infection Control 45 (11): e143 e147. doi: 10.1016/j.ajic.2017.06.002.
[9] Ekhaise, F. O., Ogboghodo, B. I (2011). Microbiological Indoor and Outdoor Air Quality of Two Major Hospitals in Benin City, Nigeria. Sierra Leone Journal of Biomedical Research 3 (3):169-174.
[10] Odigie, A. B., Ekhiase, F. O., Orjiakor, P. I., Omozuwa, S. (2017). The role of door handles in the spread of microorganisms of public health consequences in University of Benin Teaching Hospital (UBTH), Benin City, Edo State. Pharmaceutical Science and Technology 2 (2): 15-21.
[11] Cheesebrough, M. (2006). District Laboratory Practice in Tropical Countries. 2ed. Cambridge University Press, Cambridge, UK. 387pp.
[12] Turnidge, J., Peterson, D. L (2007). Setting and Revising Antibacterial Susceptibility Breakpoints. Clinical Microbiology Reviews 20 (3): 391-408.
[13] Akpi, U. K., Odoh, C. K., Ideh, E. E., Adobu, U. S (2017). Antimicrobial activity of lycoperdonperlatum whole fruit body on common pathogenic bacteria and fungi. African Journal of Clinical and Experimental Microbiology 18 (2): 79-85. doi.org/10.4314/ajcem.v18i2.4.
[14] Akpi, U. K., Odoh, C. K (2017). Antimicrobial activities of Pleurotussquarrosulus on clinical pathogenic bacteria and fungi. Journal of Advanced Microbiology 4 (3) doi: 109734/JAMB/2017/34644.
[15] Clinical Laboratory Standards Institution (CLSI) (2010). Performance Standards for Antimicrobial Susceptibility Testing. Twentieth Informational Supplemented. CLSI document Wayne, PA, USA. M100-S20. 282pp.
[16] Birnboirm H. C., Doly J (1979) “A rapid alkaline extraction procedure for screening recombinant plasmid DNA”. Nucleic Acids Research 7:1513-1523.
[17] Wasfy, M. O., Oyofo, B. A., David, J. C., Ismail, T. F., EL-Gendy, A. M., Mohran, Z. S., Sultan, Y., Peruski, L. F (2000). Isolation and antibiotic susceptibility osSalmonella, Shigella and Campylobacter from enteric infections in Egypt. Journal of Health and Population Nutrition18 (1): 33-38.
[18] Nworie, A., Ayeni, J. A., Eze, U. A., Azi, S. O (2012). Bacterial Contamination of Door Handles/Knobs in Selected Public Conveniences in Abuja Metropolis, Nigeria: A Public Health Threat. Continental Journal of Medical Research 6 (1): 7 – 11.
[19] Maori, L., Agbor, V. O., Ahmed, W. A (2013). The prevalence of bacterial organisms on toilet door handles in Secondary Schools in Bokkos L. G. A., Jos, Plateau Sate, Nigeria. IOSR Journal of Pharmacy and Biological Sciences 8 (4): 85-91.
[20] Onwubiko, N. E., Chinyeaka, A. H (2015). Isolation and identification of bacterial Contaminants from door handles in a Tertiary institution in Umuahia, Abia State, Nigeria. Nigerian Journal of Microbiology 29: 3139-3147.
[21] Aloma, A. A., Olonitola, O. S., Jatau, E. D (2016). Isolation, Characterization and Antibiotic Susceptibility Patterns of Pseudomonas aeruginosaand Staphylococcus aureusfrom Hospital Environment in Kaduna Metropolis, Kaduna State. International Journal of Scientific Research 6 (4): 141-146.
[22] Lemmen, S. W., Häfner, H., Zolldann, D., Stanzel, S., Lütticken, R (2004). Distribution of multi-resistant Gram-negative versus Gram-positive bacteria in the hospital inanimate environment. Journal of Hospital Infection 56: 191-197.
[23] Hadadi, A., Rasoulinejad, M., Maleki, Z., Yonesian, M., Shirani, A (2008). Antimicrobial resistance pattern of Gram-negative bacilli of nosocomial origin at two University hospitals in Iran. Diagnostic Microbiology and Infectious Disease 60: 301-305.
[24] Friedman, N. D., Temkin, E., Carmeli, Y (2016). The negative impact of antibiotic resistance. Clinical Microbiology and Infection 22 (5): 416-422.
[25] Atata, R. F. Ibrahim, Y. K. E. Giwa, A., Akanbi, A. A. (2013). Antibiotics resistance profile of bacterial isolates from surgical site and hospital environment in a University teaching hospital in Nigeria. Journal of Medicine and Medical Sciences 4 (4): 181-187.
[26] Mbim, E. E., Mboto, C. I., Edet, U. O (2016). Plasmid Profile Analysis and Curing of Multi-drug resistant Bacteria Isolated from Two Hospital Environments in Calabar Metropolis, Nigeria. Asian Journal of Medicine and Health 1 (1):1-11.
Cite This Article
  • APA Style

    Augustine Brian Odigie, Frederick Osaro Ekhaise, Paul Ikechukwu Orjiakor, Eze Chibuzor Nwadibe, Odeyemi Adebowale Toba, et al. (2018). Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria. Journal of Health and Environmental Research, 4(1), 35-41. https://doi.org/10.11648/j.jher.20180401.15

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    ACS Style

    Augustine Brian Odigie; Frederick Osaro Ekhaise; Paul Ikechukwu Orjiakor; Eze Chibuzor Nwadibe; Odeyemi Adebowale Toba, et al. Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria. J. Health Environ. Res. 2018, 4(1), 35-41. doi: 10.11648/j.jher.20180401.15

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    AMA Style

    Augustine Brian Odigie, Frederick Osaro Ekhaise, Paul Ikechukwu Orjiakor, Eze Chibuzor Nwadibe, Odeyemi Adebowale Toba, et al. Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria. J Health Environ Res. 2018;4(1):35-41. doi: 10.11648/j.jher.20180401.15

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  • @article{10.11648/j.jher.20180401.15,
      author = {Augustine Brian Odigie and Frederick Osaro Ekhaise and Paul Ikechukwu Orjiakor and Eze Chibuzor Nwadibe and Odeyemi Adebowale Toba and Odoh Chuks Kenneth},
      title = {Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria},
      journal = {Journal of Health and Environmental Research},
      volume = {4},
      number = {1},
      pages = {35-41},
      doi = {10.11648/j.jher.20180401.15},
      url = {https://doi.org/10.11648/j.jher.20180401.15},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jher.20180401.15},
      abstract = {In recent times, surfaces of hospital environment, constantly exposed to activities of patients and health-care workers, have been identified as potential routes for horizontal dissemination of drug resistant microorganisms of public health significance. This study was conducted to investigate the antibiotic resistance profile of bacteria isolated from samples collected from door handles of various units in University of Benin Teaching Hospital (UBTH), Benin City, Edo State, Nigeria. Out of the 66 door handles sampled and analysed bacteriologically, 68 predominant bacteria distributed among five (5) species of Escherichia coli (30.9%), Staphylococcusaureus (26.5%), Pseudomonas aeruginosa (17.6%), Streptococcus pneumonia (13.2%) and Bacillus subtilis (11.8%) were isolated. Kirby Bauer techniques of disk diffusion antibiotic susceptibility test on the isolates using 9 commercial antibiotics according to Clinical Laboratory Standards Institute (CLSI) procedure showed resistance of P. aeruginosa to five (5) drugs (Sulfamethoxazole-Trimethoprim, Streptomycin, Ampicillin, Cephalexin and Nalidixic acid), E. coli to five (5) drugs (Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin, Cephalexin and Nalidixic acid), S. pneumoniaeto two drugs (Ampicilin and Nalidixic acid) and S aureus to only Nalidixic acid. Agarose gel electrophoresis carried out on their plasmid DNA revealed detectable fragments in P. aeruginosa and E. coli. After plasmid curing, the isolates became sensitive to Streptomycin, Cephalexin,Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin and Ciprofloxacin. Findings from this study suggest the presence of plasmid mediated multi-drug resistant P. aeruginosa and E. coli on surfaces of hospital door handles. Hence, public education on proper personal hygiene and disinfection techniques are recommended in hospitals.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Antibiotic Susceptibility Profile of Bacteria Isolated from Door Handles of University of Benin Teaching Hospital, Benin City, Edo State, Nigeria
    AU  - Augustine Brian Odigie
    AU  - Frederick Osaro Ekhaise
    AU  - Paul Ikechukwu Orjiakor
    AU  - Eze Chibuzor Nwadibe
    AU  - Odeyemi Adebowale Toba
    AU  - Odoh Chuks Kenneth
    Y1  - 2018/03/23
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jher.20180401.15
    DO  - 10.11648/j.jher.20180401.15
    T2  - Journal of Health and Environmental Research
    JF  - Journal of Health and Environmental Research
    JO  - Journal of Health and Environmental Research
    SP  - 35
    EP  - 41
    PB  - Science Publishing Group
    SN  - 2472-3592
    UR  - https://doi.org/10.11648/j.jher.20180401.15
    AB  - In recent times, surfaces of hospital environment, constantly exposed to activities of patients and health-care workers, have been identified as potential routes for horizontal dissemination of drug resistant microorganisms of public health significance. This study was conducted to investigate the antibiotic resistance profile of bacteria isolated from samples collected from door handles of various units in University of Benin Teaching Hospital (UBTH), Benin City, Edo State, Nigeria. Out of the 66 door handles sampled and analysed bacteriologically, 68 predominant bacteria distributed among five (5) species of Escherichia coli (30.9%), Staphylococcusaureus (26.5%), Pseudomonas aeruginosa (17.6%), Streptococcus pneumonia (13.2%) and Bacillus subtilis (11.8%) were isolated. Kirby Bauer techniques of disk diffusion antibiotic susceptibility test on the isolates using 9 commercial antibiotics according to Clinical Laboratory Standards Institute (CLSI) procedure showed resistance of P. aeruginosa to five (5) drugs (Sulfamethoxazole-Trimethoprim, Streptomycin, Ampicillin, Cephalexin and Nalidixic acid), E. coli to five (5) drugs (Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin, Cephalexin and Nalidixic acid), S. pneumoniaeto two drugs (Ampicilin and Nalidixic acid) and S aureus to only Nalidixic acid. Agarose gel electrophoresis carried out on their plasmid DNA revealed detectable fragments in P. aeruginosa and E. coli. After plasmid curing, the isolates became sensitive to Streptomycin, Cephalexin,Ofloxacin, Sulfamethoxazole-Trimethoprim, Ampicillin and Ciprofloxacin. Findings from this study suggest the presence of plasmid mediated multi-drug resistant P. aeruginosa and E. coli on surfaces of hospital door handles. Hence, public education on proper personal hygiene and disinfection techniques are recommended in hospitals.
    VL  - 4
    IS  - 1
    ER  - 

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Author Information
  • Department of Microbiology, Faculty of Life Sciences, University of Benin, Edo State, Nigeria

  • Department of Microbiology, Faculty of Life Sciences, University of Benin, Edo State, Nigeria

  • Department of Microbiology, Faculty of Sciences, Ekiti State University, Ekiti State, Nigeria

  • Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria

  • Department of Microbiology, Faculty of Sciences, Ekiti State University, Ekiti State, Nigeria

  • Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria

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