International Journal of Animal Science and Technology

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Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms

Received: Nov. 17, 2017    Accepted: Nov. 29, 2017    Published: Jan. 17, 2018
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Abstract

A cross-sectional study was conducted from November 2014 to April 2015 to determine the prevalence of bovine mastitis, isolation of mastitis causing bacteria’s and characterize antimicrobial susceptibility pattern at Holeta Agricultural Research Centre (HARC) Dairy Farms. Purposive sampling technique was employed and all lactating cows were involved in the study. Among 186 lactating cows consists of 92 Jersey as well as 94 cross breed (Boran X Holistein Fresian) were included in the study. Among selected cows, 131 (70.43%) cows were identified as bovine mastitis positive and out of which 10 (5.37%) had clinical mastitis and 121 (65.05%) had subclinical mastitis. The different types of bacterial species isolated in present study includes S. aureus (30.93%), staphylococcus other than S. aureus (14.43%), Str. agalactiae (5.15%), Str. Dysgalactiae (5.15%), Str. Uberis (12.37%), C. bovis (13.40%), E. coli (6.18%), P. aerogenosa (10.3%) and K. pneumonie (2.05%). Antimicrobial susceptibility testing revealed that 94% and 78.6% of total isolates were susceptible to gentamycin and trimethoprim-sulfamethoxazole, respectively which was followed by erythromycin and tetracycline with susceptibility rate of 73.8% and 69%, respectively. However, penicillin had shown susceptibility of 55.9% which was the least effective among the drugs used. In conclusion, different types of mastitis causing pathogens with variable rate of susceptibility to antimicrobials were able to identify. Therefore, antimicrobial therapy should be used after isolation of the suspected pathogen to avoid emergence of drug resistance.

DOI 10.11648/j.ijast.20180201.12
Published in International Journal of Animal Science and Technology ( Volume 2, Issue 1, March 2018 )
Page(s) 6-13
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antimicrobial, Dairy, Isolation, Mastitis, Susceptibility, HARC

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Cite This Article
  • APA Style

    Kanenus Dereje Dereje, Abriham Kebede, Nigus Abebe, Yobsan Tamiru. (2018). Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms. International Journal of Animal Science and Technology, 2(1), 6-13. https://doi.org/10.11648/j.ijast.20180201.12

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    ACS Style

    Kanenus Dereje Dereje; Abriham Kebede; Nigus Abebe; Yobsan Tamiru. Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms. Int. J. Anim. Sci. Technol. 2018, 2(1), 6-13. doi: 10.11648/j.ijast.20180201.12

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    AMA Style

    Kanenus Dereje Dereje, Abriham Kebede, Nigus Abebe, Yobsan Tamiru. Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms. Int J Anim Sci Technol. 2018;2(1):6-13. doi: 10.11648/j.ijast.20180201.12

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  • @article{10.11648/j.ijast.20180201.12,
      author = {Kanenus Dereje Dereje and Abriham Kebede and Nigus Abebe and Yobsan Tamiru},
      title = {Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms},
      journal = {International Journal of Animal Science and Technology},
      volume = {2},
      number = {1},
      pages = {6-13},
      doi = {10.11648/j.ijast.20180201.12},
      url = {https://doi.org/10.11648/j.ijast.20180201.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijast.20180201.12},
      abstract = {A cross-sectional study was conducted from November 2014 to April 2015 to determine the prevalence of bovine mastitis, isolation of mastitis causing bacteria’s and characterize antimicrobial susceptibility pattern at Holeta Agricultural Research Centre (HARC) Dairy Farms. Purposive sampling technique was employed and all lactating cows were involved in the study. Among 186 lactating cows consists of 92 Jersey as well as 94 cross breed (Boran X Holistein Fresian) were included in the study. Among selected cows, 131 (70.43%) cows were identified as bovine mastitis positive and out of which 10 (5.37%) had clinical mastitis and 121 (65.05%) had subclinical mastitis. The different types of bacterial species isolated in present study includes S. aureus (30.93%), staphylococcus other than S. aureus (14.43%), Str. agalactiae (5.15%), Str. Dysgalactiae (5.15%), Str. Uberis (12.37%), C. bovis (13.40%), E. coli (6.18%), P. aerogenosa (10.3%) and K. pneumonie (2.05%). Antimicrobial susceptibility testing revealed that 94% and 78.6% of total isolates were susceptible to gentamycin and trimethoprim-sulfamethoxazole, respectively which was followed by erythromycin and tetracycline with susceptibility rate of 73.8% and 69%, respectively. However, penicillin had shown susceptibility of 55.9% which was the least effective among the drugs used. In conclusion, different types of mastitis causing pathogens with variable rate of susceptibility to antimicrobials were able to identify. Therefore, antimicrobial therapy should be used after isolation of the suspected pathogen to avoid emergence of drug resistance.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Isolation, Identification and Antimicrobial Susceptibility Test of Mastitis Causing Bacteria at Holeta Agricultural Research Center Dairy Farms
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    SN  - 2640-1312
    UR  - https://doi.org/10.11648/j.ijast.20180201.12
    AB  - A cross-sectional study was conducted from November 2014 to April 2015 to determine the prevalence of bovine mastitis, isolation of mastitis causing bacteria’s and characterize antimicrobial susceptibility pattern at Holeta Agricultural Research Centre (HARC) Dairy Farms. Purposive sampling technique was employed and all lactating cows were involved in the study. Among 186 lactating cows consists of 92 Jersey as well as 94 cross breed (Boran X Holistein Fresian) were included in the study. Among selected cows, 131 (70.43%) cows were identified as bovine mastitis positive and out of which 10 (5.37%) had clinical mastitis and 121 (65.05%) had subclinical mastitis. The different types of bacterial species isolated in present study includes S. aureus (30.93%), staphylococcus other than S. aureus (14.43%), Str. agalactiae (5.15%), Str. Dysgalactiae (5.15%), Str. Uberis (12.37%), C. bovis (13.40%), E. coli (6.18%), P. aerogenosa (10.3%) and K. pneumonie (2.05%). Antimicrobial susceptibility testing revealed that 94% and 78.6% of total isolates were susceptible to gentamycin and trimethoprim-sulfamethoxazole, respectively which was followed by erythromycin and tetracycline with susceptibility rate of 73.8% and 69%, respectively. However, penicillin had shown susceptibility of 55.9% which was the least effective among the drugs used. In conclusion, different types of mastitis causing pathogens with variable rate of susceptibility to antimicrobials were able to identify. Therefore, antimicrobial therapy should be used after isolation of the suspected pathogen to avoid emergence of drug resistance.
    VL  - 2
    IS  - 1
    ER  - 

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Author Information
  • Wama Hagalo Districts of East Wollega Zone, Oromia Region, Nekemte, Ethiopia

  • School of Veterinary Medicine, College of Medical and Health Science, Wollega University, Nekemte, Ethiopia

  • College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia

  • School of Veterinary Medicine, College of Medical and Health Science, Wollega University, Nekemte, Ethiopia

  • Section