Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in <i>Venerupis Decussata</i> Gills Collected From Two Coast Tunisian Lagoons

Safa Bejaoui; Khaoula Telahigue; Imene Chetoui; Imen Rabeh; Chaima Fouzai; Wafa Trabelsi; Ines Houas-Gharsallah; M’hamed El Cafsi; Nejla Soudani

doi:doi:10.11648/j.jcebe.20180202.12

Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons

Safa Bejaoui, Khaoula Telahigue, Imene Chetoui, Imen Rabeh, Chaima Fouzai, Wafa Trabelsi, Ines Houas-Gharsallah, M’hamed El Cafsi, Nejla Soudani

Published in Journal of Chemical, Environmental and Biological Engineering (Volume 2, Issue 2)

Received: 12 October 2018 Accepted: 26 October 2018 Published: 16 November 2018

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Abstract

The bivalve Venerupis decussata has been proposed as a sentinel species for assessment of lagoon water. Our study aimed to evaluate the spatial and temporal variation of oxidative stress biomarkers, metal content and DNA damage in Venerupis decussata digestive gland collected seasonally from contaminated (BOUGHRARA “L2”) and comparatively cleaner ( GHAR EL MELH “L1”) lagoons. Trace metal contents (Cu, Pb and Cd) in Venerupis decussata collected at polluted site were 1-2 folds higher compared to the control site and showed maximum variation especially during summer and spring seasons. The current findings indicate a seasonal increase of malondialdehyde (MDA), protein carbonyl (PCO), glutathione (GSH) and metallothioneins (MT) levels in relation to trace element accumulation in Venerupis decussata digestive gland from BOUGHRARA sampling lagoon compared to those from GHAR EL MELH. We found an increase in glutathione-S-transfers (GST) and glutathione peroxides (GPx) activities in clams collected from BOUGHRARA lagoon. A random DNA degradation was observed mostly in digestive gland from the polluted site. The principal component examination of the physiological parameters showed a clear separation between Venerupis decussata collected from the polluted lagoon (L2) and those from the clean one (L1). Our study delivers basic information on the toxicological effects of environmental pollutants in clam through the combination of metabolic and physiological methodologies.

Published in	Journal of Chemical, Environmental and Biological Engineering (Volume 2, Issue 2)
DOI	10.11648/j.jcebe.20180202.12
Page(s)	44-51
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antioxidants Defense, Bioaccumulation, Calms, DNA Damage, Gills, Lagoons

References

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Safa Bejaoui, Khaoula Telahigue, Imene Chetoui, Imen Rabeh, Chaima Fouzai, et al. (2018). Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons. Journal of Chemical, Environmental and Biological Engineering, 2(2), 44-51. https://doi.org/10.11648/j.jcebe.20180202.12

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ACS Style

Safa Bejaoui; Khaoula Telahigue; Imene Chetoui; Imen Rabeh; Chaima Fouzai, et al. Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons. J. Chem. Environ. Biol. Eng. 2018, 2(2), 44-51. doi: 10.11648/j.jcebe.20180202.12

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AMA Style

Safa Bejaoui, Khaoula Telahigue, Imene Chetoui, Imen Rabeh, Chaima Fouzai, et al. Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons. J Chem Environ Biol Eng. 2018;2(2):44-51. doi: 10.11648/j.jcebe.20180202.12

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@article{10.11648/j.jcebe.20180202.12,
  author = {Safa Bejaoui and Khaoula Telahigue and Imene Chetoui and Imen Rabeh and Chaima Fouzai and Wafa Trabelsi and Ines Houas-Gharsallah and M’hamed El Cafsi and Nejla Soudani},
  title = {Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons},
  journal = {Journal of Chemical, Environmental and Biological Engineering},
  volume = {2},
  number = {2},
  pages = {44-51},
  doi = {10.11648/j.jcebe.20180202.12},
  url = {https://doi.org/10.11648/j.jcebe.20180202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jcebe.20180202.12},
  abstract = {The bivalve Venerupis decussata has been proposed as a sentinel species for assessment of lagoon water. Our study aimed to evaluate the spatial and temporal variation of oxidative stress biomarkers, metal content and DNA damage in Venerupis decussata digestive gland collected seasonally from contaminated (BOUGHRARA “L2”) and comparatively cleaner ( GHAR EL MELH “L1”) lagoons. Trace metal contents (Cu, Pb and Cd) in Venerupis decussata collected at polluted site were 1-2 folds higher compared to the control site and showed maximum variation especially during summer and spring seasons. The current findings indicate a seasonal increase of malondialdehyde (MDA), protein carbonyl (PCO), glutathione (GSH) and metallothioneins (MT) levels in relation to trace element accumulation in Venerupis decussata digestive gland from BOUGHRARA sampling lagoon compared to those from GHAR EL MELH. We found an increase in glutathione-S-transfers (GST) and glutathione peroxides (GPx) activities in clams collected from BOUGHRARA lagoon. A random DNA degradation was observed mostly in digestive gland from the polluted site. The principal component examination of the physiological parameters showed a clear separation between Venerupis decussata collected from the polluted lagoon (L2) and those from the clean one (L1). Our study delivers basic information on the toxicological effects of environmental pollutants in clam through the combination of metabolic and physiological methodologies.},
 year = {2018}
}

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TY  - JOUR
T1  - Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons
AU  - Safa Bejaoui
AU  - Khaoula Telahigue
AU  - Imene Chetoui
AU  - Imen Rabeh
AU  - Chaima Fouzai
AU  - Wafa Trabelsi
AU  - Ines Houas-Gharsallah
AU  - M’hamed El Cafsi
AU  - Nejla Soudani
Y1  - 2018/11/16
PY  - 2018
N1  - https://doi.org/10.11648/j.jcebe.20180202.12
DO  - 10.11648/j.jcebe.20180202.12
T2  - Journal of Chemical, Environmental and Biological Engineering
JF  - Journal of Chemical, Environmental and Biological Engineering
JO  - Journal of Chemical, Environmental and Biological Engineering
SP  - 44
EP  - 51
PB  - Science Publishing Group
SN  - 2640-267X
UR  - https://doi.org/10.11648/j.jcebe.20180202.12
AB  - The bivalve Venerupis decussata has been proposed as a sentinel species for assessment of lagoon water. Our study aimed to evaluate the spatial and temporal variation of oxidative stress biomarkers, metal content and DNA damage in Venerupis decussata digestive gland collected seasonally from contaminated (BOUGHRARA “L2”) and comparatively cleaner ( GHAR EL MELH “L1”) lagoons. Trace metal contents (Cu, Pb and Cd) in Venerupis decussata collected at polluted site were 1-2 folds higher compared to the control site and showed maximum variation especially during summer and spring seasons. The current findings indicate a seasonal increase of malondialdehyde (MDA), protein carbonyl (PCO), glutathione (GSH) and metallothioneins (MT) levels in relation to trace element accumulation in Venerupis decussata digestive gland from BOUGHRARA sampling lagoon compared to those from GHAR EL MELH. We found an increase in glutathione-S-transfers (GST) and glutathione peroxides (GPx) activities in clams collected from BOUGHRARA lagoon. A random DNA degradation was observed mostly in digestive gland from the polluted site. The principal component examination of the physiological parameters showed a clear separation between Venerupis decussata collected from the polluted lagoon (L2) and those from the clean one (L1). Our study delivers basic information on the toxicological effects of environmental pollutants in clam through the combination of metabolic and physiological methodologies.
VL  - 2
IS  - 2
ER  -

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Author Information

Safa Bejaoui

Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Khaoula Telahigue

Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Imene Chetoui

Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Imen Rabeh

Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Chaima Fouzai

Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Wafa Trabelsi

Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Ines Houas-Gharsallah

Higher Institute of Marine Sciences and Technology, La Goulette Center, Tunis, Tunisia
M’hamed El Cafsi

Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia
Nejla Soudani

Department of Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia

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APA Style

Safa Bejaoui, Khaoula Telahigue, Imene Chetoui, Imen Rabeh, Chaima Fouzai, et al. (2018). Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons. Journal of Chemical, Environmental and Biological Engineering, 2(2), 44-51. https://doi.org/10.11648/j.jcebe.20180202.12

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ACS Style

Safa Bejaoui; Khaoula Telahigue; Imene Chetoui; Imen Rabeh; Chaima Fouzai, et al. Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons. J. Chem. Environ. Biol. Eng. 2018, 2(2), 44-51. doi: 10.11648/j.jcebe.20180202.12

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AMA Style

Safa Bejaoui, Khaoula Telahigue, Imene Chetoui, Imen Rabeh, Chaima Fouzai, et al. Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons. J Chem Environ Biol Eng. 2018;2(2):44-51. doi: 10.11648/j.jcebe.20180202.12

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@article{10.11648/j.jcebe.20180202.12,
  author = {Safa Bejaoui and Khaoula Telahigue and Imene Chetoui and Imen Rabeh and Chaima Fouzai and Wafa Trabelsi and Ines Houas-Gharsallah and M’hamed El Cafsi and Nejla Soudani},
  title = {Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons},
  journal = {Journal of Chemical, Environmental and Biological Engineering},
  volume = {2},
  number = {2},
  pages = {44-51},
  doi = {10.11648/j.jcebe.20180202.12},
  url = {https://doi.org/10.11648/j.jcebe.20180202.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jcebe.20180202.12},
  abstract = {The bivalve Venerupis decussata has been proposed as a sentinel species for assessment of lagoon water. Our study aimed to evaluate the spatial and temporal variation of oxidative stress biomarkers, metal content and DNA damage in Venerupis decussata digestive gland collected seasonally from contaminated (BOUGHRARA “L2”) and comparatively cleaner ( GHAR EL MELH “L1”) lagoons. Trace metal contents (Cu, Pb and Cd) in Venerupis decussata collected at polluted site were 1-2 folds higher compared to the control site and showed maximum variation especially during summer and spring seasons. The current findings indicate a seasonal increase of malondialdehyde (MDA), protein carbonyl (PCO), glutathione (GSH) and metallothioneins (MT) levels in relation to trace element accumulation in Venerupis decussata digestive gland from BOUGHRARA sampling lagoon compared to those from GHAR EL MELH. We found an increase in glutathione-S-transfers (GST) and glutathione peroxides (GPx) activities in clams collected from BOUGHRARA lagoon. A random DNA degradation was observed mostly in digestive gland from the polluted site. The principal component examination of the physiological parameters showed a clear separation between Venerupis decussata collected from the polluted lagoon (L2) and those from the clean one (L1). Our study delivers basic information on the toxicological effects of environmental pollutants in clam through the combination of metabolic and physiological methodologies.},
 year = {2018}
}

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TY  - JOUR
T1  - Integrated Effect of Metal Accumulation, Oxidative Stress Responses and DNA Damage in Venerupis Decussata Gills Collected From Two Coast Tunisian Lagoons
AU  - Safa Bejaoui
AU  - Khaoula Telahigue
AU  - Imene Chetoui
AU  - Imen Rabeh
AU  - Chaima Fouzai
AU  - Wafa Trabelsi
AU  - Ines Houas-Gharsallah
AU  - M’hamed El Cafsi
AU  - Nejla Soudani
Y1  - 2018/11/16
PY  - 2018
N1  - https://doi.org/10.11648/j.jcebe.20180202.12
DO  - 10.11648/j.jcebe.20180202.12
T2  - Journal of Chemical, Environmental and Biological Engineering
JF  - Journal of Chemical, Environmental and Biological Engineering
JO  - Journal of Chemical, Environmental and Biological Engineering
SP  - 44
EP  - 51
PB  - Science Publishing Group
SN  - 2640-267X
UR  - https://doi.org/10.11648/j.jcebe.20180202.12
AB  - The bivalve Venerupis decussata has been proposed as a sentinel species for assessment of lagoon water. Our study aimed to evaluate the spatial and temporal variation of oxidative stress biomarkers, metal content and DNA damage in Venerupis decussata digestive gland collected seasonally from contaminated (BOUGHRARA “L2”) and comparatively cleaner ( GHAR EL MELH “L1”) lagoons. Trace metal contents (Cu, Pb and Cd) in Venerupis decussata collected at polluted site were 1-2 folds higher compared to the control site and showed maximum variation especially during summer and spring seasons. The current findings indicate a seasonal increase of malondialdehyde (MDA), protein carbonyl (PCO), glutathione (GSH) and metallothioneins (MT) levels in relation to trace element accumulation in Venerupis decussata digestive gland from BOUGHRARA sampling lagoon compared to those from GHAR EL MELH. We found an increase in glutathione-S-transfers (GST) and glutathione peroxides (GPx) activities in clams collected from BOUGHRARA lagoon. A random DNA degradation was observed mostly in digestive gland from the polluted site. The principal component examination of the physiological parameters showed a clear separation between Venerupis decussata collected from the polluted lagoon (L2) and those from the clean one (L1). Our study delivers basic information on the toxicological effects of environmental pollutants in clam through the combination of metabolic and physiological methodologies.
VL  - 2
IS  - 2
ER  -

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