International Journal of Diabetes and Endocrinology

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Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss

Received: Dec. 26, 2016    Accepted: Mar. 17, 2017    Published: Apr. 11, 2017
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Abstract

Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.

DOI 10.11648/j.ijde.20170202.11
Published in International Journal of Diabetes and Endocrinology ( Volume 2, Issue 2, June 2017 )
Page(s) 19-25
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Diabetes Mellitus-2, Biochemical Parameters, MTHFR Gene Polymorphism

References
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[4] Vos, T; Flaxman, AD et al (Dec 15, 2012). "Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010.
[5] Diabetes Fact sheet N 312". WHO. 2014.
[6] Elizabeth H. Harris, clinical diabetes, 2005 American diabetes association; management of dyslipidemia in adults with diabetes.
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[11] Goyette P, Sumner JS, Milos R, Duncan AM, Rosenblatt DS, Matthews RG, Rozen R (June 1994). "Human methylenetetrahydrofolatereductase: isolation of cDNA, mapping and mutation identification". Nat. Genet. 7 (2): 195–200. doi: 10.1038/ng0694-195. PMID 7920641.
[12] Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA, Matthews RG, Boers GJ, den Heijer M, Kluijtmans LA, van den Heuvel LP et al. (May 1995). "A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolatereductase". Nat. Genet. 10 (1): 111–3. doi: 10.1038/ng0595-111. PMID 7647779.
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[15] Elizabeth H. Harris, clinical diabetes, 2005 American diabetes association; management of dyslipidemia in adults with diabetes.
[16] Movva S, Alluri RV, Venkata S, Vedicherla B, Vattam KK, Ahuja YR, Hasan Q. 2011, Association of methylene tetrahydrofolatereductase C677T genotype with type 2 diabetes mellitus. Genet Test Mol Biomarkers. 2011 Apr; 15 (4): 257-61.
[17] Wang JG, Staessen JA. Genetic polymorphisms in the renin– angiotensin system: relevance for susceptibility to cardiovascular disease. Eur J Pharmacol 2000; 410: 289–302.
[18] Weisberg IS, Jacques PF, Selhub J, et al. The 1298AfiC polymorphism in methylenetetrahydrofolatereductase (MTHFR): in vitro expression and association with homocysteine. Atherosclerosis 2001; 156: 409–15.
[19] Maeda M, Yamamoto I, Fukuda M, Motomura T, Nishida M, 2008, MTHFR gene polymorphism is susceptible to diabetic retinopathy but not to diabetic nephropathy in Japanese type 2 diabetic patients. J Diabetes Complications. 2008 Mar-Apr; 22 (2): 119-25.
[20] Sun J, Xu Y, Zhu Y, et al 2005. Methylenetetrahydrofolatereductase gene polymorphism, homocysteine and risk of macroangiopathy type 2 diabetes mellitus. J Endocrinology Invest; 29: 814–20.
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  • APA Style

    Mushtaq Ahmad Bhat, Showkat Ahmad Bhat, Sheikh Bilal Ahmad, Wasim Qureshi, Sabhiya Majid, et al. (2017). Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss. International Journal of Diabetes and Endocrinology, 2(2), 19-25. https://doi.org/10.11648/j.ijde.20170202.11

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    ACS Style

    Mushtaq Ahmad Bhat; Showkat Ahmad Bhat; Sheikh Bilal Ahmad; Wasim Qureshi; Sabhiya Majid, et al. Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss. Int. J. Diabetes Endocrinol. 2017, 2(2), 19-25. doi: 10.11648/j.ijde.20170202.11

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    AMA Style

    Mushtaq Ahmad Bhat, Showkat Ahmad Bhat, Sheikh Bilal Ahmad, Wasim Qureshi, Sabhiya Majid, et al. Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss. Int J Diabetes Endocrinol. 2017;2(2):19-25. doi: 10.11648/j.ijde.20170202.11

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  • @article{10.11648/j.ijde.20170202.11,
      author = {Mushtaq Ahmad Bhat and Showkat Ahmad Bhat and Sheikh Bilal Ahmad and Wasim Qureshi and Sabhiya Majid and Aarif Ali and Ishraq Hussain and Tehseen Hassan and Muneeb U. Rehman and Manzoor R. Mir},
      title = {Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss},
      journal = {International Journal of Diabetes and Endocrinology},
      volume = {2},
      number = {2},
      pages = {19-25},
      doi = {10.11648/j.ijde.20170202.11},
      url = {https://doi.org/10.11648/j.ijde.20170202.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijde.20170202.11},
      abstract = {Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss
    AU  - Mushtaq Ahmad Bhat
    AU  - Showkat Ahmad Bhat
    AU  - Sheikh Bilal Ahmad
    AU  - Wasim Qureshi
    AU  - Sabhiya Majid
    AU  - Aarif Ali
    AU  - Ishraq Hussain
    AU  - Tehseen Hassan
    AU  - Muneeb U. Rehman
    AU  - Manzoor R. Mir
    Y1  - 2017/04/11
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijde.20170202.11
    DO  - 10.11648/j.ijde.20170202.11
    T2  - International Journal of Diabetes and Endocrinology
    JF  - International Journal of Diabetes and Endocrinology
    JO  - International Journal of Diabetes and Endocrinology
    SP  - 19
    EP  - 25
    PB  - Science Publishing Group
    SN  - 2640-1371
    UR  - https://doi.org/10.11648/j.ijde.20170202.11
    AB  - Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.
    VL  - 2
    IS  - 2
    ER  - 

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Author Information
  • Clinical-Biochemistry, University of Kashmir, Srinagar, India

  • Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India

  • Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India

  • Registrar Academics/Physician, Govt. Medical College, Srinagar, India

  • Department of Biochemistry, Govt. Medical College, Srinagar, India

  • Clinical-Biochemistry, University of Kashmir, Srinagar, India

  • Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India

  • Department of Biochemistry, Govt. Medical College, Srinagar, India

  • Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India

  • Division of Vety. Biochemistry, F. V. Sc & AH, SKUAST-K, Shuhama Alustang, Srinagar, India

  • Section