Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR)

Maryam Derakhshan; Pegah Hedayat; Rozhan Mohammadi; Amirsajad Barahimi

doi:doi:10.11648/j.plm.20180201.14

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Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR)

Maryam Derakhshan, Pegah Hedayat, Rozhan Mohammadi, Amirsajad Barahimi

Published in Pathology and Laboratory Medicine (Volume 2, Issue 1)

Received: 22 June 2018 Accepted: 3 August 2018 Published: 10 September 2018

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Abstract

Ulcerative colitis (UC) is still controversial and recent researches referred to the role of infectious factors such as EBV in this disease, but the relationship between EBV and UC has not yet been proven. Therefore, the present study evaluates the prevalence of EBV in patients with UC and comparison with control group by Polymerase Chain Reaction (PCR). In this study, 30 samples of intestinal biopsy in patients with UC in the active phase of disease and 30 biopsy samples from non-UC subjects were evaluated by PCR method were evaluated by PCR method and the presence or absence of EBV virus in the sample was recorded. Finally, the collected data were analyzed by SPSS (Ver.22) software. As the result EBV was found in 10 biopsies of 30 from UC patients and 3 of 30 biopsies from non-UC-patients (33.3% vs. 10%, P-value = 0.028). Also, the prevalence of this virus in the both groups was not significantly different in terms of sex and age (P-value > 0.05). Therefore, it may conclude that evidence of infection of the virus in mucosal inflammatory cells of patients with UC has an important role in chronic UC. Given the small sample size, it is suggested that future studies to be carried out with similar subject in a larger population of these patients.

Published in	Pathology and Laboratory Medicine (Volume 2, Issue 1)
DOI	10.11648/j.plm.20180201.14
Page(s)	20-24
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Epstein–Barr Virus, Ulcerative Colitis, Polymerase Chain Reaction

References

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APA Style

Maryam Derakhshan, Pegah Hedayat, Rozhan Mohammadi, Amirsajad Barahimi. (2018). Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR). Pathology and Laboratory Medicine, 2(1), 20-24. https://doi.org/10.11648/j.plm.20180201.14

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ACS Style

Maryam Derakhshan; Pegah Hedayat; Rozhan Mohammadi; Amirsajad Barahimi. Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR). Pathol. Lab. Med. 2018, 2(1), 20-24. doi: 10.11648/j.plm.20180201.14

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AMA Style

Maryam Derakhshan, Pegah Hedayat, Rozhan Mohammadi, Amirsajad Barahimi. Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR). Pathol Lab Med. 2018;2(1):20-24. doi: 10.11648/j.plm.20180201.14

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@article{10.11648/j.plm.20180201.14,
  author = {Maryam Derakhshan and Pegah Hedayat and Rozhan Mohammadi and Amirsajad Barahimi},
  title = {Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR)},
  journal = {Pathology and Laboratory Medicine},
  volume = {2},
  number = {1},
  pages = {20-24},
  doi = {10.11648/j.plm.20180201.14},
  url = {https://doi.org/10.11648/j.plm.20180201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plm.20180201.14},
  abstract = {Ulcerative colitis (UC) is still controversial and recent researches referred to the role of infectious factors such as EBV in this disease, but the relationship between EBV and UC has not yet been proven. Therefore, the present study evaluates the prevalence of EBV in patients with UC and comparison with control group by Polymerase Chain Reaction (PCR). In this study, 30 samples of intestinal biopsy in patients with UC in the active phase of disease and 30 biopsy samples from non-UC subjects were evaluated by PCR method were evaluated by PCR method and the presence or absence of EBV virus in the sample was recorded. Finally, the collected data were analyzed by SPSS (Ver.22) software. As the result EBV was found in 10 biopsies of 30 from UC patients and 3 of 30 biopsies from non-UC-patients (33.3% vs. 10%, P-value = 0.028). Also, the prevalence of this virus in the both groups was not significantly different in terms of sex and age (P-value > 0.05). Therefore, it may conclude that evidence of infection of the virus in mucosal inflammatory cells of patients with UC has an important role in chronic UC. Given the small sample size, it is suggested that future studies to be carried out with similar subject in a larger population of these patients.},
 year = {2018}
}

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TY  - JOUR
T1  - Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR)
AU  - Maryam Derakhshan
AU  - Pegah Hedayat
AU  - Rozhan Mohammadi
AU  - Amirsajad Barahimi
Y1  - 2018/09/10
PY  - 2018
N1  - https://doi.org/10.11648/j.plm.20180201.14
DO  - 10.11648/j.plm.20180201.14
T2  - Pathology and Laboratory Medicine
JF  - Pathology and Laboratory Medicine
JO  - Pathology and Laboratory Medicine
SP  - 20
EP  - 24
PB  - Science Publishing Group
SN  - 2640-4478
UR  - https://doi.org/10.11648/j.plm.20180201.14
AB  - Ulcerative colitis (UC) is still controversial and recent researches referred to the role of infectious factors such as EBV in this disease, but the relationship between EBV and UC has not yet been proven. Therefore, the present study evaluates the prevalence of EBV in patients with UC and comparison with control group by Polymerase Chain Reaction (PCR). In this study, 30 samples of intestinal biopsy in patients with UC in the active phase of disease and 30 biopsy samples from non-UC subjects were evaluated by PCR method were evaluated by PCR method and the presence or absence of EBV virus in the sample was recorded. Finally, the collected data were analyzed by SPSS (Ver.22) software. As the result EBV was found in 10 biopsies of 30 from UC patients and 3 of 30 biopsies from non-UC-patients (33.3% vs. 10%, P-value = 0.028). Also, the prevalence of this virus in the both groups was not significantly different in terms of sex and age (P-value > 0.05). Therefore, it may conclude that evidence of infection of the virus in mucosal inflammatory cells of patients with UC has an important role in chronic UC. Given the small sample size, it is suggested that future studies to be carried out with similar subject in a larger population of these patients.
VL  - 2
IS  - 1
ER  -

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Author Information

Maryam Derakhshan

Department of Pathology, Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
Pegah Hedayat

Department of Pathology, Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
Rozhan Mohammadi

Department of Pathology, Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
Amirsajad Barahimi

Department of General Physician, Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran

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Plain Text BibTeX RIS

APA Style

Maryam Derakhshan, Pegah Hedayat, Rozhan Mohammadi, Amirsajad Barahimi. (2018). Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR). Pathology and Laboratory Medicine, 2(1), 20-24. https://doi.org/10.11648/j.plm.20180201.14

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ACS Style

Maryam Derakhshan; Pegah Hedayat; Rozhan Mohammadi; Amirsajad Barahimi. Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR). Pathol. Lab. Med. 2018, 2(1), 20-24. doi: 10.11648/j.plm.20180201.14

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AMA Style

Maryam Derakhshan, Pegah Hedayat, Rozhan Mohammadi, Amirsajad Barahimi. Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR). Pathol Lab Med. 2018;2(1):20-24. doi: 10.11648/j.plm.20180201.14

Copy | Download

@article{10.11648/j.plm.20180201.14,
  author = {Maryam Derakhshan and Pegah Hedayat and Rozhan Mohammadi and Amirsajad Barahimi},
  title = {Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR)},
  journal = {Pathology and Laboratory Medicine},
  volume = {2},
  number = {1},
  pages = {20-24},
  doi = {10.11648/j.plm.20180201.14},
  url = {https://doi.org/10.11648/j.plm.20180201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plm.20180201.14},
  abstract = {Ulcerative colitis (UC) is still controversial and recent researches referred to the role of infectious factors such as EBV in this disease, but the relationship between EBV and UC has not yet been proven. Therefore, the present study evaluates the prevalence of EBV in patients with UC and comparison with control group by Polymerase Chain Reaction (PCR). In this study, 30 samples of intestinal biopsy in patients with UC in the active phase of disease and 30 biopsy samples from non-UC subjects were evaluated by PCR method were evaluated by PCR method and the presence or absence of EBV virus in the sample was recorded. Finally, the collected data were analyzed by SPSS (Ver.22) software. As the result EBV was found in 10 biopsies of 30 from UC patients and 3 of 30 biopsies from non-UC-patients (33.3% vs. 10%, P-value = 0.028). Also, the prevalence of this virus in the both groups was not significantly different in terms of sex and age (P-value > 0.05). Therefore, it may conclude that evidence of infection of the virus in mucosal inflammatory cells of patients with UC has an important role in chronic UC. Given the small sample size, it is suggested that future studies to be carried out with similar subject in a larger population of these patients.},
 year = {2018}
}

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TY  - JOUR
T1  - Prevalence of Epstein–Barr Virus in Patients with Ulcerative Colitis Using Polymerase Chain Reaction (PCR)
AU  - Maryam Derakhshan
AU  - Pegah Hedayat
AU  - Rozhan Mohammadi
AU  - Amirsajad Barahimi
Y1  - 2018/09/10
PY  - 2018
N1  - https://doi.org/10.11648/j.plm.20180201.14
DO  - 10.11648/j.plm.20180201.14
T2  - Pathology and Laboratory Medicine
JF  - Pathology and Laboratory Medicine
JO  - Pathology and Laboratory Medicine
SP  - 20
EP  - 24
PB  - Science Publishing Group
SN  - 2640-4478
UR  - https://doi.org/10.11648/j.plm.20180201.14
AB  - Ulcerative colitis (UC) is still controversial and recent researches referred to the role of infectious factors such as EBV in this disease, but the relationship between EBV and UC has not yet been proven. Therefore, the present study evaluates the prevalence of EBV in patients with UC and comparison with control group by Polymerase Chain Reaction (PCR). In this study, 30 samples of intestinal biopsy in patients with UC in the active phase of disease and 30 biopsy samples from non-UC subjects were evaluated by PCR method were evaluated by PCR method and the presence or absence of EBV virus in the sample was recorded. Finally, the collected data were analyzed by SPSS (Ver.22) software. As the result EBV was found in 10 biopsies of 30 from UC patients and 3 of 30 biopsies from non-UC-patients (33.3% vs. 10%, P-value = 0.028). Also, the prevalence of this virus in the both groups was not significantly different in terms of sex and age (P-value > 0.05). Therefore, it may conclude that evidence of infection of the virus in mucosal inflammatory cells of patients with UC has an important role in chronic UC. Given the small sample size, it is suggested that future studies to be carried out with similar subject in a larger population of these patients.
VL  - 2
IS  - 1
ER  -

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