Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis

Fu Xianzhao; Huang Wenhua; Li Chunyan; Li Xingchan; Qiu Haixian; Liang Liudan; Cao Qiuxia; Liang Liuyue

doi:doi:10.11648/j.ajcem.20180605.13

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Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis

Fu Xianzhao, Huang Wenhua, Li Chunyan, Li Xingchan, Qiu Haixian, Liang Liudan, Cao Qiuxia, Liang Liuyue

Published in American Journal of Clinical and Experimental Medicine (Volume 6, Issue 5)

Received: 26 December 2018 Accepted: Published: 27 December 2018

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Abstract

Objecive: To study the effects of Huoxue Jiedu Jiangtang formulation (HJJF) on endoplasmic reticulum stress (ERS) coupled inflammatory response From the molecular mechanism in diabetic vascular disease. Methods: Type 2 diabetes were established in male SD rats by feeding high lipid diet and injection of streptozotocin. after T2DM models established successfully, the rats were divided into model group, low-dose HJJF group (HJJF1), high-dose HJJF group (HJJF2), and Western medicine group (Gliquidone+Benazepril), and accepted corresponding drugs for 8 weeks respectively. Another normal group were used as control group, fed with normal diet, no drug intervention. The levels of fasting blood glucose (FBG), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IL-6 and TNF-α were tested. The ERS signaling molecules glucose-regulated protein 78 (GRP78) and c-Jun N-terminal kinases (JNK) mRNA transcription level in thoracic aorta vessel were determined by reverse-transcription polymerase chain reaction (RT-PCR). Results: Compared with model group, After drugs intervention, all administered groups could significantly decrease FPG (P<0.05), elevate SOD and GSH-Px (P<0.05); all could reduce levels of TC, TG, LDL-C, IL-6 and TNF-α (P<0.05), and raise HDL-C (P<0.05); all could depress the transcription levels of GRP78 and JNK mRNA (P<0.05). With increasing dose, the HJJF effect is more significant (P<0.05). Conclusion: HJJF could improve insulin resistance, correct lipid metabolism disorders, and enhance the antioxidative ability, inhibit the response of ERS coupled inflammatory, showing the multi-target treatment characteristics of Traditional Chinese Medicine.

Published in	American Journal of Clinical and Experimental Medicine (Volume 6, Issue 5)
DOI	10.11648/j.ajcem.20180605.13
Page(s)	118-124
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Diabetes, Atherosclerosis, Huoxue Jiedu Jiangtang Formulation, Inflammatory Reaction, Endoplasmic Reticulum Stress

References

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[5]	FU Xian-zhao, XU Jing, LI Chun-yan, et al. Effects of Huoxue Jiedu Jiangtang formulation on the balance between pro- and anti-inflammatory cytokines in non-revascularization patients with diabetic acute coronary syndrome [J]. Chin JTCM WM Crit Care, 2016, 23 (1): 6-10.
[6]	Doultsinos D, Avril T, Lhomond S, et al. Control of the Unfolded Protein Response in Health and Disease. SLAS Discov, 2017, 22 (7): 787-800.
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[18]	Fu Xianzhao, Huang Zhenfeng, Huang Wenhua, et al. Review and prospect of using traditional Chinese medicine drugs “supplem enting Qi and nourishin g Yin, activating blood circulation and detoxifying” for prevention and treatment of diabetes mellitus complicated with acute coronary syndrome
[19]	FU Xian-zhao, XU Jing, LI Chun-yan, et al. Effect of Huoxue Jiedu Jiangtang formulation on heart remodeling in diabetic patients with acute coronary syndrome after PCI [J]. Chin J TCM WM Crit Care, 2016, 23 (2): 117-121.

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APA Style

Fu Xianzhao, Huang Wenhua, Li Chunyan, Li Xingchan, Qiu Haixian, et al. (2018). Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. American Journal of Clinical and Experimental Medicine, 6(5), 118-124. https://doi.org/10.11648/j.ajcem.20180605.13

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ACS Style

Fu Xianzhao; Huang Wenhua; Li Chunyan; Li Xingchan; Qiu Haixian, et al. Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. Am. J. Clin. Exp. Med. 2018, 6(5), 118-124. doi: 10.11648/j.ajcem.20180605.13

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AMA Style

Fu Xianzhao, Huang Wenhua, Li Chunyan, Li Xingchan, Qiu Haixian, et al. Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. Am J Clin Exp Med. 2018;6(5):118-124. doi: 10.11648/j.ajcem.20180605.13

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@article{10.11648/j.ajcem.20180605.13,
  author = {Fu Xianzhao and Huang Wenhua and Li Chunyan and Li Xingchan and Qiu Haixian and Liang Liudan and Cao Qiuxia and Liang Liuyue},
  title = {Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {6},
  number = {5},
  pages = {118-124},
  doi = {10.11648/j.ajcem.20180605.13},
  url = {https://doi.org/10.11648/j.ajcem.20180605.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20180605.13},
  abstract = {Objecive: To study the effects of Huoxue Jiedu Jiangtang formulation (HJJF) on endoplasmic reticulum stress (ERS) coupled inflammatory response From the molecular mechanism in diabetic vascular disease. Methods: Type 2 diabetes were established in male SD rats by feeding high lipid diet and injection of streptozotocin. after T2DM models established successfully, the rats were divided into model group, low-dose HJJF group (HJJF1), high-dose HJJF group (HJJF2), and Western medicine group (Gliquidone+Benazepril), and accepted corresponding drugs for 8 weeks respectively. Another normal group were used as control group, fed with normal diet, no drug intervention. The levels of fasting blood glucose (FBG), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IL-6 and TNF-α were tested. The ERS signaling molecules glucose-regulated protein 78 (GRP78) and c-Jun N-terminal kinases (JNK) mRNA transcription level in thoracic aorta vessel were determined by reverse-transcription polymerase chain reaction (RT-PCR). Results: Compared with model group, After drugs intervention, all administered groups could significantly decrease FPG (P<0.05), elevate SOD and GSH-Px (P<0.05); all could reduce levels of TC, TG, LDL-C, IL-6 and TNF-α (P<0.05), and raise HDL-C (P<0.05); all could depress the transcription levels of GRP78 and JNK mRNA (P<0.05). With increasing dose, the HJJF effect is more significant (P<0.05). Conclusion: HJJF could improve insulin resistance, correct lipid metabolism disorders, and enhance the antioxidative ability, inhibit the response of ERS coupled inflammatory, showing the multi-target treatment characteristics of Traditional Chinese Medicine.},
 year = {2018}
}

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TY  - JOUR
T1  - Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis
AU  - Fu Xianzhao
AU  - Huang Wenhua
AU  - Li Chunyan
AU  - Li Xingchan
AU  - Qiu Haixian
AU  - Liang Liudan
AU  - Cao Qiuxia
AU  - Liang Liuyue
Y1  - 2018/12/27
PY  - 2018
N1  - https://doi.org/10.11648/j.ajcem.20180605.13
DO  - 10.11648/j.ajcem.20180605.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 118
EP  - 124
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20180605.13
AB  - Objecive: To study the effects of Huoxue Jiedu Jiangtang formulation (HJJF) on endoplasmic reticulum stress (ERS) coupled inflammatory response From the molecular mechanism in diabetic vascular disease. Methods: Type 2 diabetes were established in male SD rats by feeding high lipid diet and injection of streptozotocin. after T2DM models established successfully, the rats were divided into model group, low-dose HJJF group (HJJF1), high-dose HJJF group (HJJF2), and Western medicine group (Gliquidone+Benazepril), and accepted corresponding drugs for 8 weeks respectively. Another normal group were used as control group, fed with normal diet, no drug intervention. The levels of fasting blood glucose (FBG), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IL-6 and TNF-α were tested. The ERS signaling molecules glucose-regulated protein 78 (GRP78) and c-Jun N-terminal kinases (JNK) mRNA transcription level in thoracic aorta vessel were determined by reverse-transcription polymerase chain reaction (RT-PCR). Results: Compared with model group, After drugs intervention, all administered groups could significantly decrease FPG (P<0.05), elevate SOD and GSH-Px (P<0.05); all could reduce levels of TC, TG, LDL-C, IL-6 and TNF-α (P<0.05), and raise HDL-C (P<0.05); all could depress the transcription levels of GRP78 and JNK mRNA (P<0.05). With increasing dose, the HJJF effect is more significant (P<0.05). Conclusion: HJJF could improve insulin resistance, correct lipid metabolism disorders, and enhance the antioxidative ability, inhibit the response of ERS coupled inflammatory, showing the multi-target treatment characteristics of Traditional Chinese Medicine.
VL  - 6
IS  - 5
ER  -

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Author Information

Fu Xianzhao

Clinic Medical College of Youjiang Medical National College, Baise, China
Huang Wenhua

Clinic Medical College of Youjiang Medical National College, Baise, China
Li Chunyan

Clinic Medical College of Youjiang Medical National College, Baise, China
Li Xingchan

Clinic Medical College of Youjiang Medical National College, Baise, China
Qiu Haixian

Clinic Medical College of Youjiang Medical National College, Baise, China
Liang Liudan

Clinic Medical College of Youjiang Medical National College, Baise, China
Cao Qiuxia

Clinic Medical College of Youjiang Medical National College, Baise, China
Liang Liuyue

Clinic Medical College of Youjiang Medical National College, Baise, China

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Plain Text BibTeX RIS

APA Style

Fu Xianzhao, Huang Wenhua, Li Chunyan, Li Xingchan, Qiu Haixian, et al. (2018). Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. American Journal of Clinical and Experimental Medicine, 6(5), 118-124. https://doi.org/10.11648/j.ajcem.20180605.13

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ACS Style

Fu Xianzhao; Huang Wenhua; Li Chunyan; Li Xingchan; Qiu Haixian, et al. Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. Am. J. Clin. Exp. Med. 2018, 6(5), 118-124. doi: 10.11648/j.ajcem.20180605.13

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AMA Style

Fu Xianzhao, Huang Wenhua, Li Chunyan, Li Xingchan, Qiu Haixian, et al. Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis. Am J Clin Exp Med. 2018;6(5):118-124. doi: 10.11648/j.ajcem.20180605.13

Copy | Download

@article{10.11648/j.ajcem.20180605.13,
  author = {Fu Xianzhao and Huang Wenhua and Li Chunyan and Li Xingchan and Qiu Haixian and Liang Liudan and Cao Qiuxia and Liang Liuyue},
  title = {Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {6},
  number = {5},
  pages = {118-124},
  doi = {10.11648/j.ajcem.20180605.13},
  url = {https://doi.org/10.11648/j.ajcem.20180605.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20180605.13},
  abstract = {Objecive: To study the effects of Huoxue Jiedu Jiangtang formulation (HJJF) on endoplasmic reticulum stress (ERS) coupled inflammatory response From the molecular mechanism in diabetic vascular disease. Methods: Type 2 diabetes were established in male SD rats by feeding high lipid diet and injection of streptozotocin. after T2DM models established successfully, the rats were divided into model group, low-dose HJJF group (HJJF1), high-dose HJJF group (HJJF2), and Western medicine group (Gliquidone+Benazepril), and accepted corresponding drugs for 8 weeks respectively. Another normal group were used as control group, fed with normal diet, no drug intervention. The levels of fasting blood glucose (FBG), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IL-6 and TNF-α were tested. The ERS signaling molecules glucose-regulated protein 78 (GRP78) and c-Jun N-terminal kinases (JNK) mRNA transcription level in thoracic aorta vessel were determined by reverse-transcription polymerase chain reaction (RT-PCR). Results: Compared with model group, After drugs intervention, all administered groups could significantly decrease FPG (P<0.05), elevate SOD and GSH-Px (P<0.05); all could reduce levels of TC, TG, LDL-C, IL-6 and TNF-α (P<0.05), and raise HDL-C (P<0.05); all could depress the transcription levels of GRP78 and JNK mRNA (P<0.05). With increasing dose, the HJJF effect is more significant (P<0.05). Conclusion: HJJF could improve insulin resistance, correct lipid metabolism disorders, and enhance the antioxidative ability, inhibit the response of ERS coupled inflammatory, showing the multi-target treatment characteristics of Traditional Chinese Medicine.},
 year = {2018}
}

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TY  - JOUR
T1  - Effect of Huoxue Jiedu Jiangtang Formulation on Endoplasmic Reticulum Stress in Diabetic Atherosclerosis
AU  - Fu Xianzhao
AU  - Huang Wenhua
AU  - Li Chunyan
AU  - Li Xingchan
AU  - Qiu Haixian
AU  - Liang Liudan
AU  - Cao Qiuxia
AU  - Liang Liuyue
Y1  - 2018/12/27
PY  - 2018
N1  - https://doi.org/10.11648/j.ajcem.20180605.13
DO  - 10.11648/j.ajcem.20180605.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 118
EP  - 124
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20180605.13
AB  - Objecive: To study the effects of Huoxue Jiedu Jiangtang formulation (HJJF) on endoplasmic reticulum stress (ERS) coupled inflammatory response From the molecular mechanism in diabetic vascular disease. Methods: Type 2 diabetes were established in male SD rats by feeding high lipid diet and injection of streptozotocin. after T2DM models established successfully, the rats were divided into model group, low-dose HJJF group (HJJF1), high-dose HJJF group (HJJF2), and Western medicine group (Gliquidone+Benazepril), and accepted corresponding drugs for 8 weeks respectively. Another normal group were used as control group, fed with normal diet, no drug intervention. The levels of fasting blood glucose (FBG), serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), IL-6 and TNF-α were tested. The ERS signaling molecules glucose-regulated protein 78 (GRP78) and c-Jun N-terminal kinases (JNK) mRNA transcription level in thoracic aorta vessel were determined by reverse-transcription polymerase chain reaction (RT-PCR). Results: Compared with model group, After drugs intervention, all administered groups could significantly decrease FPG (P<0.05), elevate SOD and GSH-Px (P<0.05); all could reduce levels of TC, TG, LDL-C, IL-6 and TNF-α (P<0.05), and raise HDL-C (P<0.05); all could depress the transcription levels of GRP78 and JNK mRNA (P<0.05). With increasing dose, the HJJF effect is more significant (P<0.05). Conclusion: HJJF could improve insulin resistance, correct lipid metabolism disorders, and enhance the antioxidative ability, inhibit the response of ERS coupled inflammatory, showing the multi-target treatment characteristics of Traditional Chinese Medicine.
VL  - 6
IS  - 5
ER  -

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