American Journal of Laboratory Medicine

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The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria

Received: May 26, 2018    Accepted: Dec. 25, 2018    Published: Jan. 17, 2019
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Abstract

The study determined the effects of highly active antiretroviral therapy on the levels of Superoxide dismutase, catalase and C-reactive protein HIV-infected subjects in Nigeria. A total of 50 HIV infected subjects aged 20-69 (39±10) years and 50 HIV seronegative control participantsaged 21-56 (35±10) years were recruited for the study. Blood samples were collected at 3 different points: before initiation of HAART, 6 months and 12 months into HAART. The serum levels of C - reactive protein (CRP), catalase, Superoxide Dismutase (SOD), CD4 + T cells and viral load counts were measured in these subjects before HAART initiation and at 6 and 12 months after HAART intake. Standard laboratory methods were used in the analysis of these parameters. The results showed that CRP was significantly increased in HIV infected subjects before commencement of HAART and remained significantly increased after 12 months intake of HAART compared to control participants (P<0.01)respectively. SOD, and CD4 were significantly lower before HAART initiation and after 12 months intake of HAART compared to control participants (P<0.01) respectively. Viral load was significantly reduced after 12 months intake of HAART. There was a negative correlation between the viral load and SOD (r=-0.41, P<0.01) and catalase (r=-0.47, P<0.01) and a positive correlation between the viral load and CRP (r=0.48, P<0.01) before HAART initiation. After 12 months of HAART catalase showed a significant negative correlation with viral load (r=-0.37, P<0.05) while CRP showed a positive correlation with viral load (r=0.33, P<0.05). The study shows a persistently elevated CRP and reduced SOD and catalase after 12 months intake of HAART. These biomarkers support a central role of inflammation and oxidative stress in HIV pathogenesis.

DOI 10.11648/j.ajlm.20180306.12
Published in American Journal of Laboratory Medicine ( Volume 3, Issue 6, November 2018 )
Page(s) 53-59
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Highly Active Antiretroviral Therapy, HIV, Catalase, SOD, C - Reactive Protein

References
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Cite This Article
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    Faustina Nkechi Osuji, Charles Chinedum Onyenekwe, Nkeiruka Rose Ukaibe, Joseph Ebere Ahaneku. (2019). The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria. American Journal of Laboratory Medicine, 3(6), 53-59. https://doi.org/10.11648/j.ajlm.20180306.12

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    ACS Style

    Faustina Nkechi Osuji; Charles Chinedum Onyenekwe; Nkeiruka Rose Ukaibe; Joseph Ebere Ahaneku. The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria. Am. J. Lab. Med. 2019, 3(6), 53-59. doi: 10.11648/j.ajlm.20180306.12

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    AMA Style

    Faustina Nkechi Osuji, Charles Chinedum Onyenekwe, Nkeiruka Rose Ukaibe, Joseph Ebere Ahaneku. The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria. Am J Lab Med. 2019;3(6):53-59. doi: 10.11648/j.ajlm.20180306.12

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  • @article{10.11648/j.ajlm.20180306.12,
      author = {Faustina Nkechi Osuji and Charles Chinedum Onyenekwe and Nkeiruka Rose Ukaibe and Joseph Ebere Ahaneku},
      title = {The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria},
      journal = {American Journal of Laboratory Medicine},
      volume = {3},
      number = {6},
      pages = {53-59},
      doi = {10.11648/j.ajlm.20180306.12},
      url = {https://doi.org/10.11648/j.ajlm.20180306.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajlm.20180306.12},
      abstract = {The study determined the effects of highly active antiretroviral therapy on the levels of Superoxide dismutase, catalase and C-reactive protein HIV-infected subjects in Nigeria. A total of 50 HIV infected subjects aged 20-69 (39±10) years and 50 HIV seronegative control participantsaged 21-56 (35±10) years were recruited for the study. Blood samples were collected at 3 different points: before initiation of HAART, 6 months and 12 months into HAART. The serum levels of C - reactive protein (CRP), catalase, Superoxide Dismutase (SOD), CD4 + T cells and viral load counts were measured in these subjects before HAART initiation and at 6 and 12 months after HAART intake. Standard laboratory methods were used in the analysis of these parameters. The results showed that CRP was significantly increased in HIV infected subjects before commencement of HAART and remained significantly increased after 12 months intake of HAART compared to control participants (P<0.01)respectively. SOD, and CD4 were significantly lower before HAART initiation and after 12 months intake of HAART compared to control participants (P<0.01) respectively. Viral load was significantly reduced after 12 months intake of HAART. There was a negative correlation between the viral load and SOD (r=-0.41, P<0.01) and catalase (r=-0.47, P<0.01) and a positive correlation between the viral load and CRP (r=0.48, P<0.01) before HAART initiation. After 12 months of HAART catalase showed a significant negative correlation with viral load (r=-0.37, P<0.05) while CRP showed a positive correlation with viral load (r=0.33, P<0.05). The study shows a persistently elevated CRP and reduced SOD and catalase after 12 months intake of HAART. These biomarkers support a central role of inflammation and oxidative stress in HIV pathogenesis.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria
    AU  - Faustina Nkechi Osuji
    AU  - Charles Chinedum Onyenekwe
    AU  - Nkeiruka Rose Ukaibe
    AU  - Joseph Ebere Ahaneku
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    N1  - https://doi.org/10.11648/j.ajlm.20180306.12
    DO  - 10.11648/j.ajlm.20180306.12
    T2  - American Journal of Laboratory Medicine
    JF  - American Journal of Laboratory Medicine
    JO  - American Journal of Laboratory Medicine
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    EP  - 59
    PB  - Science Publishing Group
    SN  - 2575-386X
    UR  - https://doi.org/10.11648/j.ajlm.20180306.12
    AB  - The study determined the effects of highly active antiretroviral therapy on the levels of Superoxide dismutase, catalase and C-reactive protein HIV-infected subjects in Nigeria. A total of 50 HIV infected subjects aged 20-69 (39±10) years and 50 HIV seronegative control participantsaged 21-56 (35±10) years were recruited for the study. Blood samples were collected at 3 different points: before initiation of HAART, 6 months and 12 months into HAART. The serum levels of C - reactive protein (CRP), catalase, Superoxide Dismutase (SOD), CD4 + T cells and viral load counts were measured in these subjects before HAART initiation and at 6 and 12 months after HAART intake. Standard laboratory methods were used in the analysis of these parameters. The results showed that CRP was significantly increased in HIV infected subjects before commencement of HAART and remained significantly increased after 12 months intake of HAART compared to control participants (P<0.01)respectively. SOD, and CD4 were significantly lower before HAART initiation and after 12 months intake of HAART compared to control participants (P<0.01) respectively. Viral load was significantly reduced after 12 months intake of HAART. There was a negative correlation between the viral load and SOD (r=-0.41, P<0.01) and catalase (r=-0.47, P<0.01) and a positive correlation between the viral load and CRP (r=0.48, P<0.01) before HAART initiation. After 12 months of HAART catalase showed a significant negative correlation with viral load (r=-0.37, P<0.05) while CRP showed a positive correlation with viral load (r=0.33, P<0.05). The study shows a persistently elevated CRP and reduced SOD and catalase after 12 months intake of HAART. These biomarkers support a central role of inflammation and oxidative stress in HIV pathogenesis.
    VL  - 3
    IS  - 6
    ER  - 

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Author Information
  • Laboratory Department, Immaculate Heart Specialist Hospital, Nkpor-Agu, Nigeria

  • Department of Medical Laboratory Science, NnamdiAzikiwe University, Nnewi Campus, Nigeria

  • Department of Medical Laboratory Science, NnamdiAzikiwe University, Nnewi Campus, Nigeria

  • Department of Chemical Pathology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria

  • Section