Screening and Characterization of Fungal Taxol from Leaf Spot Fungi

Bose Chitra Kani; Senthuran Suresh Kumar; Mohan Pandi

doi:doi:10.11648/j.bio.20170506.11

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Screening and Characterization of Fungal Taxol from Leaf Spot Fungi

Bose Chitra Kani, Senthuran Suresh Kumar, Mohan Pandi

Published in American Journal of Bioscience and Bioengineering (Volume 5, Issue 6)

Received: 1 November 2017 Accepted: 9 November 2017 Published: 6 December 2017

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Abstract

Plants are the tremendous source for the discovery of new biotherapeutic products with medicinal importance in drug development. Paclitaxel (Taxol) is a plant-derived natural product that exhibits potential anticancer activity. Taxol binding specifically with β-tubulin and prevents depolymerisation during the process of cell division in cancer cells as anticancer property. This billion dollar drug was first isolated in trace amounts (1kg taxol recovered from 13,500kg of bark) from the bark of Yew tree (Taxus brevifolia), but huge drawback is production rate was low and shortage of yew trees. Currently, endophytic fungi and pathogenic fungi are the best alternative source for the taxol production. In this study, twenty-seven different plants were used for infected leaves collection from Madurai Kamaraj University (MKU) campus. Fungi were isolated from infected leaf spots on PDA plates using hyphal tip method. All the isolated fungi were subjected to pathogenicity tests, and positive pathogenic fungi were re-isolated. Totally eighteen fungal plant pathogens were isolated. The positive isolates identified using morphological and molecular characters. All the positive pathogens are screened preliminarily for taxol production. Among the eighteen isolates, three fungi were produced taxol. The test fungus Phoma moricola, isolated from Bauhinia tomentosa was the first report for Taxol production. The amount of taxol produced by Phoma moricola was quantified by HPLC. The maximum amount of taxol produced was found to be 302μg/L.

Published in	American Journal of Bioscience and Bioengineering (Volume 5, Issue 6)
DOI	10.11648/j.bio.20170506.11
Page(s)	113-120
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Paclitaxel (Taxol), Leaf Spot Fungus, Phoma moricola

References

[1]	Sim J, Horng, HC Khoo, HL Lee, KY Cheah, Molecular diversity of fungal endophytes isolated from Garcinia mangostana and Garcinia parvifolia. 2010; J. Microbiol. Biotechnol. 20(4) 651-658.
[2]	Kumaran R. S, J Muthumary, Eun- Ki, Kim and Byung-Ki Hur. 2009; roduction of Taxol from Phyllosticta dioscoreae, A leaf spot fungus isolated from Hibiscus Rosa Sinensis. Biotechnology and Bioprocess Engineering. (14): 76-83.
[3]	Kumaran RS, J Muthumary, BK Hur. 2008; Taxol from Phyllosticta citricarpa, a leaf spot fungus of the angiosperm Citrus medica. J Biosci Bioeng. 106(1): 103-6.10.
[4]	Kirk D, Kevin Hyde, EHC Saisamorn Lumyong, McKenzie and EB Gareth Jones. The Occurrence of fungi on tissues of the peat swamp palm Licualalongi calycata. 2001; Fungal Diversity 25: 157-173.
[5]	Guo-Yin T, Y Zhi-Ling, Z Shou–An. Morphological, Molecular and pathogenic characterization of Alternaria longipes, The fungal Pathogen Causing Leaf Spot On Atractylodes macrocephala. 2013; Afr. J. Microbiol. Res. 7 (21): 2589-2595.
[6]	Suh C, M Chrysayi-Tokousbalides, N Fokialakis and A Konstantinos. 2014; Pathogenicity of Fusarium oxysporum schlecht. F. sp. Orthoceras strain isolated from Orobanchecrenata. African advances in agricultural research. 1 (1): 11-22.
[7]	Gokul Raj K, N Sundaresan, EG Jagan, G Rajapriya, P Muthumary, J Sridhar and M Pandi 2014; Phylogenetic reconstruction of endophytic fungal isolates using internal transcribed spacer-2(ITS 2) Bioinformation. 10 (6): 320-328.
[8]	Strobel G. A, X Yang, J Sears, R Kramer, R. S Sidhu, And WM Hess, Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. 1996; Microbiol. (142) 435-440.
[9]	Chakravarthi BVSK, P Das, K Surendranath, K Karande. Production of paclitaxel by Fusarium solani isolated from Taxus celebica. 2008; J. Biosci. (33): 259-267.
[10]	Li Y, GA Strobel, R Sidhu, W M Hess, EJ Ford. Endophytic taxol-producing fungi from bald cypress, Taxodium distichum. 1996; Microbiol. 142 (8) 2223 -2226.
[11]	Wani M. C, HL Taylor, ME Wall, P Coggon and AT McPhail, Plant antitumor agents IV, The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. 1971; J. Am. Chem. Soc. (93) 2325-2347.
[12]	Holmes F A, RS Walters, RL Theriault, AD Forman, LK Newton, MN Raber, AU Buzdar, D K Frye, G N Hortobagyi. 1991; Phase II trial of taxol, an active drug in the treatment of metastatic breast cancer.
[13]	Rowinsky EK, RC Donehower. 1995; Paclitaxel (taxol) N Engl J Med; 333(1): 75.
[14]	Gangadevi V, J Muthumary. Taxol production by Pestalotiopsis terminaliae, an endophytic fungus of Terminalia arjuna (arjun tree). 2009; Biotechnol. Appl. Biochem. (52)9-1.
[15]	Pandi M, RS Kumaran, YK Choi, HJ Kim, J Muthumary. Isolation, and detection of taxol, an anticancer drug produced from Lasiodiplodia theobromae, an endophytic fungus of the medicinal plant Morinda citrifolia. 2011; Afr J Biotechnol 10: 1428-1435.
[16]	Sunpapao A, J Morakul and C Suriy. 2014; Identification of curvularia orzae as cause of leaf spot disease on oil palm seedlings in nurseries of Thailand. Phytopara. 1-12.
[17]	Kathiravan, GSM Sureban, HN Sree, V Bhuvaneshwari and E Kramony. Isolation of anticancer drug taxol from Pestalotiopsis breviseta with apoptosis amd B –Cell lymphoma protein docking studies. 2013; Basic. Cin. Pharma. (4) 14-9.
[18]	Kumaran RS, and HB Kim and B Hur Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspiata. 2011; Biosci. Bioeng. 110 (5)541-546.
[19]	Li J, Y Hu, W Chen and Z Lin. Identification and pilot study of Taxus endophytic fungi’s taxol – producing correlation gene BAPT. 2006; Biotechnol. 356-371.
[20]	Merlin J. N, I V S Nimal Christhudas, P Kumar, M Kumar and A Agastian, Taxol production by endophytic Fusarium solani LCPANCF01 from Tylophora indica. 2012; J. Acad. Indus. (5)1.
[21]	Wise K. 2010; Diseases of corn gray leaf spot. www.btny.purdue.edu.Bp:56-W.

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APA Style

Bose Chitra Kani, Senthuran Suresh Kumar, Mohan Pandi. (2017). Screening and Characterization of Fungal Taxol from Leaf Spot Fungi. American Journal of Bioscience and Bioengineering, 5(6), 113-120. https://doi.org/10.11648/j.bio.20170506.11

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ACS Style

Bose Chitra Kani; Senthuran Suresh Kumar; Mohan Pandi. Screening and Characterization of Fungal Taxol from Leaf Spot Fungi. Am. J. BioSci. Bioeng. 2017, 5(6), 113-120. doi: 10.11648/j.bio.20170506.11

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AMA Style

Bose Chitra Kani, Senthuran Suresh Kumar, Mohan Pandi. Screening and Characterization of Fungal Taxol from Leaf Spot Fungi. Am J BioSci Bioeng. 2017;5(6):113-120. doi: 10.11648/j.bio.20170506.11

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@article{10.11648/j.bio.20170506.11,
  author = {Bose Chitra Kani and Senthuran Suresh Kumar and Mohan Pandi},
  title = {Screening and Characterization of Fungal Taxol from Leaf Spot Fungi},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {5},
  number = {6},
  pages = {113-120},
  doi = {10.11648/j.bio.20170506.11},
  url = {https://doi.org/10.11648/j.bio.20170506.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20170506.11},
  abstract = {Plants are the tremendous source for the discovery of new biotherapeutic products with medicinal importance in drug development. Paclitaxel (Taxol) is a plant-derived natural product that exhibits potential anticancer activity. Taxol binding specifically with β-tubulin and prevents depolymerisation during the process of cell division in cancer cells as anticancer property. This billion dollar drug was first isolated in trace amounts (1kg taxol recovered from 13,500kg of bark) from the bark of Yew tree (Taxus brevifolia), but huge drawback is production rate was low and shortage of yew trees. Currently, endophytic fungi and pathogenic fungi are the best alternative source for the taxol production. In this study, twenty-seven different plants were used for infected leaves collection from Madurai Kamaraj University (MKU) campus. Fungi were isolated from infected leaf spots on PDA plates using hyphal tip method. All the isolated fungi were subjected to pathogenicity tests, and positive pathogenic fungi were re-isolated. Totally eighteen fungal plant pathogens were isolated. The positive isolates identified using morphological and molecular characters. All the positive pathogens are screened preliminarily for taxol production. Among the eighteen isolates, three fungi were produced taxol. The test fungus Phoma moricola, isolated from Bauhinia tomentosa was the first report for Taxol production. The amount of taxol produced by Phoma moricola was quantified by HPLC. The maximum amount of taxol produced was found to be 302μg/L.},
 year = {2017}
}

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TY - JOUR
T1 - Screening and Characterization of Fungal Taxol from Leaf Spot Fungi
AU - Bose Chitra Kani
AU - Senthuran Suresh Kumar
AU - Mohan Pandi
Y1 - 2017/12/06
PY - 2017
N1 - https://doi.org/10.11648/j.bio.20170506.11
DO - 10.11648/j.bio.20170506.11
T2 - American Journal of Bioscience and Bioengineering
JF - American Journal of Bioscience and Bioengineering
JO - American Journal of Bioscience and Bioengineering
SP - 113
EP - 120
PB - Science Publishing Group
SN - 2328-5893
UR - https://doi.org/10.11648/j.bio.20170506.11
AB - Plants are the tremendous source for the discovery of new biotherapeutic products with medicinal importance in drug development. Paclitaxel (Taxol) is a plant-derived natural product that exhibits potential anticancer activity. Taxol binding specifically with β-tubulin and prevents depolymerisation during the process of cell division in cancer cells as anticancer property. This billion dollar drug was first isolated in trace amounts (1kg taxol recovered from 13,500kg of bark) from the bark of Yew tree (Taxus brevifolia), but huge drawback is production rate was low and shortage of yew trees. Currently, endophytic fungi and pathogenic fungi are the best alternative source for the taxol production. In this study, twenty-seven different plants were used for infected leaves collection from Madurai Kamaraj University (MKU) campus. Fungi were isolated from infected leaf spots on PDA plates using hyphal tip method. All the isolated fungi were subjected to pathogenicity tests, and positive pathogenic fungi were re-isolated. Totally eighteen fungal plant pathogens were isolated. The positive isolates identified using morphological and molecular characters. All the positive pathogens are screened preliminarily for taxol production. Among the eighteen isolates, three fungi were produced taxol. The test fungus Phoma moricola, isolated from Bauhinia tomentosa was the first report for Taxol production. The amount of taxol produced by Phoma moricola was quantified by HPLC. The maximum amount of taxol produced was found to be 302μg/L.
VL - 5
IS - 6
ER -

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Author Information

Bose Chitra Kani

Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu
Senthuran Suresh Kumar

Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu
Mohan Pandi

Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu

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APA Style

Bose Chitra Kani, Senthuran Suresh Kumar, Mohan Pandi. (2017). Screening and Characterization of Fungal Taxol from Leaf Spot Fungi. American Journal of Bioscience and Bioengineering, 5(6), 113-120. https://doi.org/10.11648/j.bio.20170506.11

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ACS Style

Bose Chitra Kani; Senthuran Suresh Kumar; Mohan Pandi. Screening and Characterization of Fungal Taxol from Leaf Spot Fungi. Am. J. BioSci. Bioeng. 2017, 5(6), 113-120. doi: 10.11648/j.bio.20170506.11

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AMA Style

Bose Chitra Kani, Senthuran Suresh Kumar, Mohan Pandi. Screening and Characterization of Fungal Taxol from Leaf Spot Fungi. Am J BioSci Bioeng. 2017;5(6):113-120. doi: 10.11648/j.bio.20170506.11

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@article{10.11648/j.bio.20170506.11,
  author = {Bose Chitra Kani and Senthuran Suresh Kumar and Mohan Pandi},
  title = {Screening and Characterization of Fungal Taxol from Leaf Spot Fungi},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {5},
  number = {6},
  pages = {113-120},
  doi = {10.11648/j.bio.20170506.11},
  url = {https://doi.org/10.11648/j.bio.20170506.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20170506.11},
  abstract = {Plants are the tremendous source for the discovery of new biotherapeutic products with medicinal importance in drug development. Paclitaxel (Taxol) is a plant-derived natural product that exhibits potential anticancer activity. Taxol binding specifically with β-tubulin and prevents depolymerisation during the process of cell division in cancer cells as anticancer property. This billion dollar drug was first isolated in trace amounts (1kg taxol recovered from 13,500kg of bark) from the bark of Yew tree (Taxus brevifolia), but huge drawback is production rate was low and shortage of yew trees. Currently, endophytic fungi and pathogenic fungi are the best alternative source for the taxol production. In this study, twenty-seven different plants were used for infected leaves collection from Madurai Kamaraj University (MKU) campus. Fungi were isolated from infected leaf spots on PDA plates using hyphal tip method. All the isolated fungi were subjected to pathogenicity tests, and positive pathogenic fungi were re-isolated. Totally eighteen fungal plant pathogens were isolated. The positive isolates identified using morphological and molecular characters. All the positive pathogens are screened preliminarily for taxol production. Among the eighteen isolates, three fungi were produced taxol. The test fungus Phoma moricola, isolated from Bauhinia tomentosa was the first report for Taxol production. The amount of taxol produced by Phoma moricola was quantified by HPLC. The maximum amount of taxol produced was found to be 302μg/L.},
 year = {2017}
}

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