Comparative Isolation of <i>Escherichia coli</i> 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria

Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Upla Peter Uteh; Odonye Dauda Dantani; Akharenegbe Pedro; Fadayomi Victor Kolawole; Uchenwa Mercy Ogechi

doi:doi:10.11648/j.abb.20180602.12

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Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Upla Peter Uteh, Odonye Dauda Dantani, Akharenegbe Pedro, Fadayomi Victor Kolawole, Uchenwa Mercy Ogechi

Received: 10 June 2018 Accepted: 4 July 2018 Published: 15 August 2018

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Abstract

Escherichia coli O157:H7 has been considered an emerging foodborne pathogen causing severe diarrheal disease globally especially among children under the age of five years in Sub-Saharan Africa. This study was aimed at conducting a comparative study on the rate of isolation of Escherichia coli O157:H7 from diarrhoeic and non- diarrhoeic children in selected communities in Cross River State, Nigeria. Stool samples were collected from children under the age of five yrs and the pathogen isolated and identified using standard microbiological and biochemical procedures. Serological analysis to detect E. coli 0157:H7 serotype was carried out using Enzyme-linked immunosorbent assay (ELISA) and anti-rabbit H7 latex serum agglutination techniques. Out of 367 diarrhoeic children sampled, 70 (19.07%) were positive for E. coli 0157:H7 and the prevalence differed significantly (p<0.05) with the control. The prevalence of E. coli 0157:H7 between diarrhoeal (19.07%) and non-diarrhoeal (1.39%) cases also differed significantly (p<0.05) among the sampling areas. Diarrhoeric children below the age of one year had significantly highest prevalence of 26.83% at p<0.05 though no significant relationship between the sex of the children and the rate of infection with the organism was observed. Children diarrhoaeic stool therefore serves as a major vehicle in the domestic transmission of Escherichia coli O157:H7. Improved personal hygiene and environmental sanitation among parents and care givers can reduce the spread of diarrheal disease caused by this pathogen amongst children under the age of five years.

DOI	10.11648/j.abb.20180602.12
Published in	Advances in Bioscience and Bioengineering (Volume 6, Issue 2, June 2018)
Page(s)	23-29
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Escherichia coli O157:H7, Childhood Diarrhoea, Nigeria

References

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APA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Upla Peter Uteh, Odonye Dauda Dantani, Akharenegbe Pedro, et al. (2018). Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria. Advances in Bioscience and Bioengineering, 6(2), 23-29. https://doi.org/10.11648/j.abb.20180602.12

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ACS Style

Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Upla Peter Uteh; Odonye Dauda Dantani; Akharenegbe Pedro, et al. Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria. Adv. BioSci. Bioeng. 2018, 6(2), 23-29. doi: 10.11648/j.abb.20180602.12

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AMA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Upla Peter Uteh, Odonye Dauda Dantani, Akharenegbe Pedro, et al. Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria. Adv BioSci Bioeng. 2018;6(2):23-29. doi: 10.11648/j.abb.20180602.12

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@article{10.11648/j.abb.20180602.12,
  author = {Nfongeh Joseph Fuh and Owoseni Mojisola Christiana and Upla Peter Uteh and Odonye Dauda Dantani and Akharenegbe Pedro and Fadayomi Victor Kolawole and Uchenwa Mercy Ogechi},
  title = {Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {6},
  number = {2},
  pages = {23-29},
  doi = {10.11648/j.abb.20180602.12},
  url = {https://doi.org/10.11648/j.abb.20180602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20180602.12},
  abstract = {Escherichia coli O157:H7 has been considered an emerging foodborne pathogen causing severe diarrheal disease globally especially among children under the age of five years in Sub-Saharan Africa. This study was aimed at conducting a comparative study on the rate of isolation of Escherichia coli O157:H7 from diarrhoeic and non- diarrhoeic children in selected communities in Cross River State, Nigeria. Stool samples were collected from children under the age of five yrs and the pathogen isolated and identified using standard microbiological and biochemical procedures. Serological analysis to detect E. coli 0157:H7 serotype was carried out using Enzyme-linked immunosorbent assay (ELISA) and anti-rabbit H7 latex serum agglutination techniques. Out of 367 diarrhoeic children sampled, 70 (19.07%) were positive for E. coli 0157:H7 and the prevalence differed significantly (pE. coli 0157:H7 between diarrhoeal (19.07%) and non-diarrhoeal (1.39%) cases also differed significantly (pEscherichia coli O157:H7. Improved personal hygiene and environmental sanitation among parents and care givers can reduce the spread of diarrheal disease caused by this pathogen amongst children under the age of five years.},
 year = {2018}
}

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TY  - JOUR
T1  - Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria
AU  - Nfongeh Joseph Fuh
AU  - Owoseni Mojisola Christiana
AU  - Upla Peter Uteh
AU  - Odonye Dauda Dantani
AU  - Akharenegbe Pedro
AU  - Fadayomi Victor Kolawole
AU  - Uchenwa Mercy Ogechi
Y1  - 2018/08/15
PY  - 2018
N1  - https://doi.org/10.11648/j.abb.20180602.12
DO  - 10.11648/j.abb.20180602.12
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 23
EP  - 29
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20180602.12
AB  - Escherichia coli O157:H7 has been considered an emerging foodborne pathogen causing severe diarrheal disease globally especially among children under the age of five years in Sub-Saharan Africa. This study was aimed at conducting a comparative study on the rate of isolation of Escherichia coli O157:H7 from diarrhoeic and non- diarrhoeic children in selected communities in Cross River State, Nigeria. Stool samples were collected from children under the age of five yrs and the pathogen isolated and identified using standard microbiological and biochemical procedures. Serological analysis to detect E. coli 0157:H7 serotype was carried out using Enzyme-linked immunosorbent assay (ELISA) and anti-rabbit H7 latex serum agglutination techniques. Out of 367 diarrhoeic children sampled, 70 (19.07%) were positive for E. coli 0157:H7 and the prevalence differed significantly (pE. coli 0157:H7 between diarrhoeal (19.07%) and non-diarrhoeal (1.39%) cases also differed significantly (pEscherichia coli O157:H7. Improved personal hygiene and environmental sanitation among parents and care givers can reduce the spread of diarrheal disease caused by this pathogen amongst children under the age of five years.
VL  - 6
IS  - 2
ER  -

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Author Information

Nfongeh Joseph Fuh

Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Owoseni Mojisola Christiana

Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Upla Peter Uteh

Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Odonye Dauda Dantani

Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Akharenegbe Pedro

Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Fadayomi Victor Kolawole

Department of Microbiology, Faculty of Science, Federal University, Lafia, Nigeria
Uchenwa Mercy Ogechi

Department of Microbiology, Faculty of Biological Sciences, University of Calabar, Calabar, Nigeria

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APA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Upla Peter Uteh, Odonye Dauda Dantani, Akharenegbe Pedro, et al. (2018). Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria. Advances in Bioscience and Bioengineering, 6(2), 23-29. https://doi.org/10.11648/j.abb.20180602.12

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ACS Style

Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Upla Peter Uteh; Odonye Dauda Dantani; Akharenegbe Pedro, et al. Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria. Adv. BioSci. Bioeng. 2018, 6(2), 23-29. doi: 10.11648/j.abb.20180602.12

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AMA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Upla Peter Uteh, Odonye Dauda Dantani, Akharenegbe Pedro, et al. Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria. Adv BioSci Bioeng. 2018;6(2):23-29. doi: 10.11648/j.abb.20180602.12

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@article{10.11648/j.abb.20180602.12,
  author = {Nfongeh Joseph Fuh and Owoseni Mojisola Christiana and Upla Peter Uteh and Odonye Dauda Dantani and Akharenegbe Pedro and Fadayomi Victor Kolawole and Uchenwa Mercy Ogechi},
  title = {Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {6},
  number = {2},
  pages = {23-29},
  doi = {10.11648/j.abb.20180602.12},
  url = {https://doi.org/10.11648/j.abb.20180602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20180602.12},
  abstract = {Escherichia coli O157:H7 has been considered an emerging foodborne pathogen causing severe diarrheal disease globally especially among children under the age of five years in Sub-Saharan Africa. This study was aimed at conducting a comparative study on the rate of isolation of Escherichia coli O157:H7 from diarrhoeic and non- diarrhoeic children in selected communities in Cross River State, Nigeria. Stool samples were collected from children under the age of five yrs and the pathogen isolated and identified using standard microbiological and biochemical procedures. Serological analysis to detect E. coli 0157:H7 serotype was carried out using Enzyme-linked immunosorbent assay (ELISA) and anti-rabbit H7 latex serum agglutination techniques. Out of 367 diarrhoeic children sampled, 70 (19.07%) were positive for E. coli 0157:H7 and the prevalence differed significantly (pE. coli 0157:H7 between diarrhoeal (19.07%) and non-diarrhoeal (1.39%) cases also differed significantly (pEscherichia coli O157:H7. Improved personal hygiene and environmental sanitation among parents and care givers can reduce the spread of diarrheal disease caused by this pathogen amongst children under the age of five years.},
 year = {2018}
}

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TY  - JOUR
T1  - Comparative Isolation of Escherichia coli 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria
AU  - Nfongeh Joseph Fuh
AU  - Owoseni Mojisola Christiana
AU  - Upla Peter Uteh
AU  - Odonye Dauda Dantani
AU  - Akharenegbe Pedro
AU  - Fadayomi Victor Kolawole
AU  - Uchenwa Mercy Ogechi
Y1  - 2018/08/15
PY  - 2018
N1  - https://doi.org/10.11648/j.abb.20180602.12
DO  - 10.11648/j.abb.20180602.12
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 23
EP  - 29
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20180602.12
AB  - Escherichia coli O157:H7 has been considered an emerging foodborne pathogen causing severe diarrheal disease globally especially among children under the age of five years in Sub-Saharan Africa. This study was aimed at conducting a comparative study on the rate of isolation of Escherichia coli O157:H7 from diarrhoeic and non- diarrhoeic children in selected communities in Cross River State, Nigeria. Stool samples were collected from children under the age of five yrs and the pathogen isolated and identified using standard microbiological and biochemical procedures. Serological analysis to detect E. coli 0157:H7 serotype was carried out using Enzyme-linked immunosorbent assay (ELISA) and anti-rabbit H7 latex serum agglutination techniques. Out of 367 diarrhoeic children sampled, 70 (19.07%) were positive for E. coli 0157:H7 and the prevalence differed significantly (pE. coli 0157:H7 between diarrhoeal (19.07%) and non-diarrhoeal (1.39%) cases also differed significantly (pEscherichia coli O157:H7. Improved personal hygiene and environmental sanitation among parents and care givers can reduce the spread of diarrheal disease caused by this pathogen amongst children under the age of five years.
VL  - 6
IS  - 2
ER  -

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