International Journal of Environmental Monitoring and Analysis

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The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library

Received: Jan. 03, 2019    Accepted: Jan. 31, 2019    Published: Sep. 10, 2019
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Abstract

The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.

DOI 10.11648/j.ijema.20190702.12
Published in International Journal of Environmental Monitoring and Analysis ( Volume 7, Issue 2, April 2019 )
Page(s) 48-55
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Mangrove Wetland, Ecological System, Microbial Diversity, 16S rDNA Library, Microbial Resources, Gene Resource

References
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[3] Hamzah T N T, Lee S Y, Hidayat A, et al. 2018. Diversity and characterization of endophytic fungi isolated from the tropical mangrove species, rhizophora mucronata, and identification of potential antagonists against the soil-borne fungus, fusarium solani. Frontiers in Microbiology, 9, 1707.
[4] Alongi D M. 1988. Bacterial productivity and microbial biomass in tropical mangrove sediments. Microbial Ecology, 15 (1), 59-79.
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[8] Handelsman J. 2004. Metagenomics: application of genomics to uncultured microorganisms. Microbiology and Molecular Biology Reviews, 68 (4), 669-85.
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[16] Zhou Z, Meng H, Liu Y, et al. 2017. Stratified bacterial and archaeal community in mangrove and intertidal wetland mudflats revealed by high throughput 16S rRNA gene sequencing. Frontiers in Microbiology, 8, 2148.
[17] Ward D M, Weller R, Bateson M M. 1990. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature, 345, 63-65.
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    Huijie Liu, Yun Tian. (2019). The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library. International Journal of Environmental Monitoring and Analysis, 7(2), 48-55. https://doi.org/10.11648/j.ijema.20190702.12

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    Huijie Liu; Yun Tian. The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library. Int. J. Environ. Monit. Anal. 2019, 7(2), 48-55. doi: 10.11648/j.ijema.20190702.12

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    AMA Style

    Huijie Liu, Yun Tian. The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library. Int J Environ Monit Anal. 2019;7(2):48-55. doi: 10.11648/j.ijema.20190702.12

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  • @article{10.11648/j.ijema.20190702.12,
      author = {Huijie Liu and Yun Tian},
      title = {The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library},
      journal = {International Journal of Environmental Monitoring and Analysis},
      volume = {7},
      number = {2},
      pages = {48-55},
      doi = {10.11648/j.ijema.20190702.12},
      url = {https://doi.org/10.11648/j.ijema.20190702.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijema.20190702.12},
      abstract = {The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library
    AU  - Huijie Liu
    AU  - Yun Tian
    Y1  - 2019/09/10
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    N1  - https://doi.org/10.11648/j.ijema.20190702.12
    DO  - 10.11648/j.ijema.20190702.12
    T2  - International Journal of Environmental Monitoring and Analysis
    JF  - International Journal of Environmental Monitoring and Analysis
    JO  - International Journal of Environmental Monitoring and Analysis
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    PB  - Science Publishing Group
    SN  - 2328-7667
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    AB  - The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.
    VL  - 7
    IS  - 2
    ER  - 

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Author Information
  • Guangzhou Military Area Environmental Monitoring Station, Guangzhou, China

  • State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China

  • Section