Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer

Stephen Emmanuel; Okwute Loretta Ojonoma; Idoko Peter Arome; Makolo Daniel

doi:doi:10.11648/j.ijema.20160401.14

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Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer

Stephen Emmanuel, Okwute Loretta Ojonoma, Idoko Peter Arome, Makolo Daniel

Published in International Journal of Environmental Monitoring and Analysis (Volume 4, Issue 1)

Received: 7 November 2015 Accepted: 19 November 2015 Published: 1 February 2016

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Abstract

Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×10⁴ – 6.8 ×10⁴ cfu/g), polluted soil (2.2×10⁴ – 9.8×10⁴ cfu/g) and Amended soil (1.62×10⁴ – 1.12×10⁵ cfu/g). The fungi counts range were Oil free soil (2.5×10³ – 3.0×10⁴ cfu/g), polluted soil (2.2×10³ – 4.0×10⁴ cfu/g) and Amended soil (2.1×10³ – 2.7×10^{3/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.}

Published in	International Journal of Environmental Monitoring and Analysis (Volume 4, Issue 1)
DOI	10.11648/j.ijema.20160401.14
Page(s)	21-26
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Lime, Polluted Soil, Biodegradation, Inoculation

References

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APA Style

Stephen Emmanuel, Okwute Loretta Ojonoma, Idoko Peter Arome, Makolo Daniel. (2016). Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer. International Journal of Environmental Monitoring and Analysis, 4(1), 21-26. https://doi.org/10.11648/j.ijema.20160401.14

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ACS Style

Stephen Emmanuel; Okwute Loretta Ojonoma; Idoko Peter Arome; Makolo Daniel. Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer. Int. J. Environ. Monit. Anal. 2016, 4(1), 21-26. doi: 10.11648/j.ijema.20160401.14

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AMA Style

Stephen Emmanuel, Okwute Loretta Ojonoma, Idoko Peter Arome, Makolo Daniel. Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer. Int J Environ Monit Anal. 2016;4(1):21-26. doi: 10.11648/j.ijema.20160401.14

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@article{10.11648/j.ijema.20160401.14,
  author = {Stephen Emmanuel and Okwute Loretta Ojonoma and Idoko Peter Arome and Makolo Daniel},
  title = {Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer},
  journal = {International Journal of Environmental Monitoring and Analysis},
  volume = {4},
  number = {1},
  pages = {21-26},
  doi = {10.11648/j.ijema.20160401.14},
  url = {https://doi.org/10.11648/j.ijema.20160401.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijema.20160401.14},
  abstract = {Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×104 – 6.8 ×104 cfu/g), polluted soil (2.2×104 – 9.8×104 cfu/g) and Amended soil (1.62×104 – 1.12×105 cfu/g). The fungi counts range were Oil free soil (2.5×103 – 3.0×104 cfu/g), polluted soil (2.2×103 – 4.0×104 cfu/g) and Amended soil (2.1×103 – 2.7×103/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.},
 year = {2016}
}

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TY  - JOUR
T1  - Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer
AU  - Stephen Emmanuel
AU  - Okwute Loretta Ojonoma
AU  - Idoko Peter Arome
AU  - Makolo Daniel
Y1  - 2016/02/01
PY  - 2016
N1  - https://doi.org/10.11648/j.ijema.20160401.14
DO  - 10.11648/j.ijema.20160401.14
T2  - International Journal of Environmental Monitoring and Analysis
JF  - International Journal of Environmental Monitoring and Analysis
JO  - International Journal of Environmental Monitoring and Analysis
SP  - 21
EP  - 26
PB  - Science Publishing Group
SN  - 2328-7667
UR  - https://doi.org/10.11648/j.ijema.20160401.14
AB  - Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×104 – 6.8 ×104 cfu/g), polluted soil (2.2×104 – 9.8×104 cfu/g) and Amended soil (1.62×104 – 1.12×105 cfu/g). The fungi counts range were Oil free soil (2.5×103 – 3.0×104 cfu/g), polluted soil (2.2×103 – 4.0×104 cfu/g) and Amended soil (2.1×103 – 2.7×103/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.
VL  - 4
IS  - 1
ER  -

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Author Information

Stephen Emmanuel

Department of Microbiology, Kogi State University, Anyigba-Kogi State, Nigeria
Okwute Loretta Ojonoma

Department of Microbiology, University of Abuja, Gwagwalada- Abuja, Nigeria
Idoko Peter Arome

Department of Microbiology, Kogi State University, Anyigba-Kogi State, Nigeria
Makolo Daniel

School of Preliminary Studies, Kogi State Polytechnic, Kogi State, Nigeria

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APA Style

Stephen Emmanuel, Okwute Loretta Ojonoma, Idoko Peter Arome, Makolo Daniel. (2016). Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer. International Journal of Environmental Monitoring and Analysis, 4(1), 21-26. https://doi.org/10.11648/j.ijema.20160401.14

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ACS Style

Stephen Emmanuel; Okwute Loretta Ojonoma; Idoko Peter Arome; Makolo Daniel. Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer. Int. J. Environ. Monit. Anal. 2016, 4(1), 21-26. doi: 10.11648/j.ijema.20160401.14

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AMA Style

Stephen Emmanuel, Okwute Loretta Ojonoma, Idoko Peter Arome, Makolo Daniel. Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer. Int J Environ Monit Anal. 2016;4(1):21-26. doi: 10.11648/j.ijema.20160401.14

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@article{10.11648/j.ijema.20160401.14,
  author = {Stephen Emmanuel and Okwute Loretta Ojonoma and Idoko Peter Arome and Makolo Daniel},
  title = {Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer},
  journal = {International Journal of Environmental Monitoring and Analysis},
  volume = {4},
  number = {1},
  pages = {21-26},
  doi = {10.11648/j.ijema.20160401.14},
  url = {https://doi.org/10.11648/j.ijema.20160401.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijema.20160401.14},
  abstract = {Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×104 – 6.8 ×104 cfu/g), polluted soil (2.2×104 – 9.8×104 cfu/g) and Amended soil (1.62×104 – 1.12×105 cfu/g). The fungi counts range were Oil free soil (2.5×103 – 3.0×104 cfu/g), polluted soil (2.2×103 – 4.0×104 cfu/g) and Amended soil (2.1×103 – 2.7×103/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.},
 year = {2016}
}

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TY  - JOUR
T1  - Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer
AU  - Stephen Emmanuel
AU  - Okwute Loretta Ojonoma
AU  - Idoko Peter Arome
AU  - Makolo Daniel
Y1  - 2016/02/01
PY  - 2016
N1  - https://doi.org/10.11648/j.ijema.20160401.14
DO  - 10.11648/j.ijema.20160401.14
T2  - International Journal of Environmental Monitoring and Analysis
JF  - International Journal of Environmental Monitoring and Analysis
JO  - International Journal of Environmental Monitoring and Analysis
SP  - 21
EP  - 26
PB  - Science Publishing Group
SN  - 2328-7667
UR  - https://doi.org/10.11648/j.ijema.20160401.14
AB  - Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×104 – 6.8 ×104 cfu/g), polluted soil (2.2×104 – 9.8×104 cfu/g) and Amended soil (1.62×104 – 1.12×105 cfu/g). The fungi counts range were Oil free soil (2.5×103 – 3.0×104 cfu/g), polluted soil (2.2×103 – 4.0×104 cfu/g) and Amended soil (2.1×103 – 2.7×103/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.
VL  - 4
IS  - 1
ER  -

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