Science Journal of Clinical Medicine

| Peer-Reviewed |

Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory

Received: Mar. 09, 2018    Accepted: Apr. 02, 2018    Published: May 11, 2018
Views:       Downloads:

Share This Article

Abstract

Wound infections cause clinical and societal consequences on the patients, but its bacteriological characteristicvaries with different factors. Therefore, effective treatment and management of wound infections in hospital and communitysetting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiogramunusual to theenvironment. Based on this information, we examined the frequency and antibiogram of bacterial pathogens isolated from woundinfection cases seen at ArshoAdvancedmedical laboratory over the study period. A total of 259 wound swabs/ and pus of different types of woundinfections from different anatomical sites were analyzed by standard bacteriological methods. Of the 259 clinical specimens analyzed, 177 (68.4%) yielded at least one bacterial pathogens, 6(2.3%) were polymicrobial, and 82(31.6%) yielded no bacterial growth. Overall, 20 different bacterial pathogens were identified 15 (75%) gram-negative bacteria isolates and 5(25%) gram-positive bacterial isolated. Staphylococcus aureus accounted formajority of the bacterial pathogens isolated, 86 (48.6% followed by E.coli20 (11.3%, and Citrobacterspp. 17(9.6%). The bacterial pathogens demonstrated high resistance to amoxicillin (79.7 %%), ampicillin (78.3%), and tetracycline (73.1%), in contrastto high sensitivity pattern observed with Meropinem (94.5%), Levofloxacin (87%), Amikacin (82.4%), and Ceftazidime (72.7%). Amikacin, meropenem and levofloxacin were the most effective drugs against the tested gram- positive and -negative bacteria and should be considered in empirical antibiotic selection.

DOI 10.11648/j.sjcm.20180702.12
Published in Science Journal of Clinical Medicine ( Volume 7, Issue 2, March 2018 )
Page(s) 20-24
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Wound Infections, Bacterial Pathogens, Polymicrobial, Arsho

References
[1] Esebelahie, N. O. Newton-Esebelahie, F. O. Omoregie R. Aerobic bacteria isolated from infected wound. African journal of clinical and expremental microbiology. 2013; 14(3):1595-689.
[2] Egbe CA, Omoregie R, Igbarumah IO, Onemu S. Microbiology of wound infections and its associated risk factors among patients of a Tertiary hospital in Benin City, Nigeria. JRHS. 2011; 11(2):109-113.
[3] Bowler PG, Duerden BI and Armstrong DG. Wound microbiology and associated approaches to wound management. Clinical Microbiology Review. 2001; 14:244-269.
[4] Sibor JO, Oseni A, Eyaufe A, Osagie R. Turay A. Incidence of aerobic bacteria and Candida albicas in post operative wound infections. Afr J Microbiol Res. 2008; 2:288-291.
[5] Aynalem Mohammed, Mengistu Endris Seid, Teklay Gebrecherkos, Moges Tiruneh, and Feleke Moges, “Bacterial Isolates and Their Antimicrobial Susceptibility Patterns of Wound Infections among Inpatients and Outpatients Attending the University of Gondar Referral Hospital, Northwest Ethiopia,” International Journal of Microbiology, 2017, 2017; 10.
[6] Cheesbrough M. Microbiology: in Medical Laboratory Manual for Tropical countries. ELBS edition. University Press, Cambridge. 1999; 132: 26-58.
[7] Bauer, A. W., W. M. Kirby, J. C. Sherris and M. Turck. Antibiotic susceptibility testing by a standard single discmethod. American Journal Clinical Pathology. 1966; 45:493-496.
[8] Rao R, Basu R, Biswas DR. Aerobic Bacterial Profile and Antimicrobial Susceptibility Pattern ofPusIsolates in a South Indian Tertiary Care Hospital. IOSR-JDMS. 2014; 13(3):59-62.
[9] Bularafa Mohammed Yasidiet al.: Retrospective Analysis of Bacterial Pathogens Isolated from Wound Infections at a Tertiary Hospital in Nguru, Yobe State Nigeria.
[10] Khan I, Sarwar N, Ahmad B, Azam S, Rehman N Identification and Antimicrobial Susceptibility Profile of Bacterial Pathogens Isolated From Wound Infections in a Teaching Hospital, Peshawar, Pakistan. Adv. Life Sci. 2017; 5(1): 08-12.
[11] Karia JB, Gadekar HB, Lakhani SJ. Study of bacterial profile of pus culture in dhirajGeneralhospital. Indian, J. SurgOncol. 2013; 4(2):172–218.
[12] Shittu AO, Kolawole DO and Oyedepo EAR. A study ofwound infections in two health institutions in ile-ife, Nigeria. Afr. J. Biomed. Res. 2002; 5 (3): 97–102.
[13] Muluye D, Wondimeneh Y, Ferede G, Nega T, Adane K, Biadgo B, Tesfa H, Moges. Bacterial isolates and their antibiotic susceptibility patterns among patients with pus and/or wound discharge at Gondar University hospital. BMC Research Notes. 2014; 7:619.
[14] [14] Daniel SJC, Gowthani DE, Sowmiya S. Isolation and identification of bcateril pathogens from wound of diabetic patients. Int. J. Curr, Microbiol. Appl. Sci. 2013; 2(11):72-77.
[15] Adane Bitew, Mesele Admassie, Tigist Getachew. Spectrum and Drug Susceptibility Profile of Bacteria Recovered from Patients with wound Infection Referred to Arsho Advanced Medical Laboratory. Clinical Medicine Research. 2018;7 (1): 8-17.
[16] Manikandan C. and Amsath A. Antibiotic susceptibility of bacterial strains isolated from wound infection patients in Pattukkottai, Tamilnadu. India Int. J. Curr. Microbiol. App. Sci. 2013; 2(6): 195-203.
[17] Syed AsadAli, S. M. Tahir, Abdul SattarMemon, Noshad A. Shaikh. Pattern of pathogens and their sensitivity isolated from superficial surgical site infections in a tertiary care hospital. JAyub Med Coll Abbottabad. 2009; 21(2).
[18] Agwunglefah, F. D., Nwabunike, C. C. and Nwaju, P. C. Antibiotic Susceptibility Pattern of Bacteria Isolated From Surgical Wounds of Patients Attending Federal Medical Center and Christiana Specialist Hospital, Owerri. Journal of Natural Sciences Research. 2014; 4(15): 2224-3186.
Cite This Article
  • APA Style

    Messele Admassie, Estifanos Tsige, Meseret Chanie. (2018). Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory. Science Journal of Clinical Medicine, 7(2), 20-24. https://doi.org/10.11648/j.sjcm.20180702.12

    Copy | Download

    ACS Style

    Messele Admassie; Estifanos Tsige; Meseret Chanie. Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory. Sci. J. Clin. Med. 2018, 7(2), 20-24. doi: 10.11648/j.sjcm.20180702.12

    Copy | Download

    AMA Style

    Messele Admassie, Estifanos Tsige, Meseret Chanie. Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory. Sci J Clin Med. 2018;7(2):20-24. doi: 10.11648/j.sjcm.20180702.12

    Copy | Download

  • @article{10.11648/j.sjcm.20180702.12,
      author = {Messele Admassie and Estifanos Tsige and Meseret Chanie},
      title = {Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory},
      journal = {Science Journal of Clinical Medicine},
      volume = {7},
      number = {2},
      pages = {20-24},
      doi = {10.11648/j.sjcm.20180702.12},
      url = {https://doi.org/10.11648/j.sjcm.20180702.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.sjcm.20180702.12},
      abstract = {Wound infections cause clinical and societal consequences on the patients, but its bacteriological characteristicvaries with different factors. Therefore, effective treatment and management of wound infections in hospital and communitysetting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiogramunusual to theenvironment. Based on this information, we examined the frequency and antibiogram of bacterial pathogens isolated from woundinfection cases seen at ArshoAdvancedmedical laboratory over the study period. A total of 259 wound swabs/ and pus of different types of woundinfections from different anatomical sites were analyzed by standard bacteriological methods. Of the 259 clinical specimens analyzed, 177 (68.4%) yielded at least one bacterial pathogens, 6(2.3%) were polymicrobial, and 82(31.6%) yielded no bacterial growth. Overall, 20 different bacterial pathogens were identified 15 (75%) gram-negative bacteria isolates and 5(25%) gram-positive bacterial isolated. Staphylococcus aureus accounted formajority of the bacterial pathogens isolated, 86 (48.6% followed by E.coli20 (11.3%, and Citrobacterspp. 17(9.6%). The bacterial pathogens demonstrated high resistance to amoxicillin (79.7 %%), ampicillin (78.3%), and tetracycline (73.1%), in contrastto high sensitivity pattern observed with Meropinem (94.5%), Levofloxacin (87%), Amikacin (82.4%), and Ceftazidime (72.7%). Amikacin, meropenem and levofloxacin were the most effective drugs against the tested gram- positive and -negative bacteria and should be considered in empirical antibiotic selection.},
     year = {2018}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Isolation, Identification and Antibiotic Susceptibility Pattern of Bacteria Isolated from Wounds of Patients Attending at Arsho Advanced Medical Laboratory
    AU  - Messele Admassie
    AU  - Estifanos Tsige
    AU  - Meseret Chanie
    Y1  - 2018/05/11
    PY  - 2018
    N1  - https://doi.org/10.11648/j.sjcm.20180702.12
    DO  - 10.11648/j.sjcm.20180702.12
    T2  - Science Journal of Clinical Medicine
    JF  - Science Journal of Clinical Medicine
    JO  - Science Journal of Clinical Medicine
    SP  - 20
    EP  - 24
    PB  - Science Publishing Group
    SN  - 2327-2732
    UR  - https://doi.org/10.11648/j.sjcm.20180702.12
    AB  - Wound infections cause clinical and societal consequences on the patients, but its bacteriological characteristicvaries with different factors. Therefore, effective treatment and management of wound infections in hospital and communitysetting will require detailed epidemiological knowledge of the infecting bacterial pathogens and their antibiogramunusual to theenvironment. Based on this information, we examined the frequency and antibiogram of bacterial pathogens isolated from woundinfection cases seen at ArshoAdvancedmedical laboratory over the study period. A total of 259 wound swabs/ and pus of different types of woundinfections from different anatomical sites were analyzed by standard bacteriological methods. Of the 259 clinical specimens analyzed, 177 (68.4%) yielded at least one bacterial pathogens, 6(2.3%) were polymicrobial, and 82(31.6%) yielded no bacterial growth. Overall, 20 different bacterial pathogens were identified 15 (75%) gram-negative bacteria isolates and 5(25%) gram-positive bacterial isolated. Staphylococcus aureus accounted formajority of the bacterial pathogens isolated, 86 (48.6% followed by E.coli20 (11.3%, and Citrobacterspp. 17(9.6%). The bacterial pathogens demonstrated high resistance to amoxicillin (79.7 %%), ampicillin (78.3%), and tetracycline (73.1%), in contrastto high sensitivity pattern observed with Meropinem (94.5%), Levofloxacin (87%), Amikacin (82.4%), and Ceftazidime (72.7%). Amikacin, meropenem and levofloxacin were the most effective drugs against the tested gram- positive and -negative bacteria and should be considered in empirical antibiotic selection.
    VL  - 7
    IS  - 2
    ER  - 

    Copy | Download

Author Information
  • Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia

  • Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia

  • Department of Microbiology, Arsho Advanced Medical Laboratory, Addis Ababa, Ethiopia

  • Section