International Journal of Nutrition and Food Sciences

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Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro

Received: Apr. 07, 2018    Accepted: Apr. 27, 2018    Published: May 18, 2018
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Abstract

Thiamine and quinine are popular bitter substances and their physiological effects have been studied; however, their impact on digestion remains unknown. Here, the physiological effects of thiamine and quinine was investigated for in vitro contraction of mouse ileum. Acetylcholine stimulates autonomous contraction of mouse ileum in a dose-dependent manner. The effect of Acetylcholine for contraction of ileum was partly suppressed by the adrenaline administration. Upon simultaneous treatment of the ileum by acetylcholine, thiamine, and quinine decreased the maximum contraction. The period till half maximum contraction was prolonged by the presence of thiamine and quinine but not by adrenaline. Because a physiological effect of thiamine and quinine was observed on acetylcholine-induced contraction of the ileum, the repertoire of human bitter taste receptors, TAS2R-1, -4, -7, -10, -14, -31, -39, -40, -43, and -46, were investigated to which thiamine and quinine may bind. These human bitter taste receptors were further analyzed among the database for mouse homologs using evolutionally conserved amino acid sequences. The only bitter receptor for both thiamine and quinine was TAS2R-39, the homology of TAS2R-139 to human TAS2R-39 was 74%. Importantly, the homology of mouse TAS2R-119 to human TAS2R-1 which interact with thiamine was 91%, and that of TAS2R-130 to human TAS2R-7 that interact with quinine was 81%. The present study indicated that thiamine and quinine changed the early phase of contraction of ileum in mice and suggested that TAS2R119 and TAS2R130 expressed in mouse enteroendocrine cells to modify the physiological effects of thiamine and quinine on the acetylcholine-induced contraction of the ileum.

DOI 10.11648/j.ijnfs.20180703.13
Published in International Journal of Nutrition and Food Sciences ( Volume 7, Issue 3, May 2018 )
Page(s) 94-99
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Thiamine, Quinine, Movement, Small Intestine, Mouse

References
[1] Nei, M., Niimura, Y., Nozawa, M. (2008). The evolution of animal chemosensory receptor gene repertoires: roles of chance and necessity. Nature Reviews Genetics 9:951–963.
[2] Liman, E. R., Zhang, Y. V., Montell, C. (2014). Peripheral coding of taste. Neuron, 81: 984–1000.
[3] Chandrashekar, J., Hoon, M. A., Ryba, N. J., Zuker, C. S. (2006). The receptors and cells for mammalian taste. Nature 444:288–894.
[4] Zhang, Y., Hoon, M. A., Chandrashekar, J., Mueller, K. L., Cook, B., Wu, D., Zuker, C. S., Ryba, N. J. (2003). Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. Cell 112: 293–301.
[5] Chaudhari, N., Roper, S. D. (2010). The cell biology of taste. J. Cell Biol. 190:285–896.
[6] Bachmanov, A. A., Bosak, N. P., Lin, C., Matsumoto, I., Ohmoto, M., Reed, D. R., Nelson, T. M. (2014). Genetics of Taste Receptors. Current Pharmaceutical Design, 20: 2669–2683.
[7] Mennella, J. A., Spector, A. C., Reed, D. R., Coldwell, S. E. (2013). The Bad Taste of Medicines: Overview of Basic Research on Bitter Taste. Clinical Therapeutics, 35:1225–1246.
[8] Santa-Cruz Calvo, S., Egan, J. M. (2015). The endocrinology of taste receptors. Nature Reviews. Endocrinology, 11:213–227.
[9] Ota, M. S., Kaneko, Y., Kondo, K., Ogishima, S., Tanaka, H., Eto, K., Kondo, T. (2009). Combined in silico and in vivo analyses reveal role of Hes1 in taste cell differentiation. PLoS Genetics. 5, e1000443.
[10] Behrens, M., Meyerho, W. (2011). Gustatory and extragustatory functions of mammalian taste receptors. Physiol. Behavior 105:4–13.
[11] Loper, H. B., Sala, M. L., Dotson, C., Steinle, N. (2015). Taste perception, associated hormonal modulation, and nutrient intake. Nutr Rev. 73: 83–91.
[12] Reimann, F., Tolhurst, G., Gribble, F. M. (2012). G-protein-coupled receptors in intestinal chemo-sensation. Cell Metabolism 15:421–431.
[13] Jaggupilli A., Howard R., Upadhyaya, J. D., Bhullar, R. P., Chelikani, P. (2016). Bitter taste receptors: Novel insights into the biochemistry and pharmacology. Int. J. Biochem. Cell Biol. 77:184–196.
[14] Shaik, F. A., Singh, N., Arakawa, M., Duan, K., Bhullar, R. P., Chelikani, P. (2016). Bitter taste receptors: extraoral roles in pathophysiology. Int. J. Biochem. Cell Biol. 77:197–204.
[15] Lee, R. J., Xiong, G., Kofonow, J. M., Chen, B., Lysenko A., Jiang P., Abraham, V., Doghramji, L., Adappa, N. D., Palmer, J. N., Kennedy, D. W., Beauchamp, G. K., Doulias P. T., Ischiropoulos, H., Kreindler, J. L., Reed D. R., Cohen, N. A. (2012). T2R38 taste receptor polymorphisms underlie susceptibility to upper respiratory infection. J. Clinical Invest.122:4145–4159.
[16] Zhang, C. H., Lifshitz, L. M., Uy, K. F., Ikebe, M., Fogarty, K. E., ZhuGe, R. (2013). The cellular and molecular basis of bitter tastant-induced bronchodilation. PLoS Biol. 11, e1001501
[17] Avau, B., Depoortere. I. (2016). The bitter truth about bitter taste receptors: beyond sensing bitter in the oral cavity. Acta Physiol. 216: 407–420.
[18] Deckmann, K., Filipski, K., Krasteva-Christ, G., Fronius, M., Althaus, M., Rafiq, A., Papadakis, T., Renno, L., Jurastow, I., Wessels, L., Wolff, M., Schütz, B., Weihe, E., Chubanov, V., Gudermann, T., Klein, J., Bschleipfer, T., Kummer, W. (2014). Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes. Proc. Natl. Acad. Sci. U S A. 111: 8287–8292.
[19] Sanders K. M.,, Koh, S. D., Ro, S., Ward, S. M. (2012). Regulation of gastrointestinal motility- insights from smooth muscle biology. Nature Reviews: Gastroenterol. Hepatol. 9:633–645.
[20] Hagger, R., Finlayson, C., Jeffrey, I., Kumar, D.(1997). Role of the interstitial cells of Cajal in the control of gut motility. Br. J. Surg. 84: 445–450.
[21] Takaki, M. (2003). Gut pacemaker cells: the interstitial cells of Cajal (ICC). J. Smooth Muscle Res. 39:137–161.
[22] Baker, S. A., Drumm, B. T., Skowronek, K. E., Rembetski, B. E., Peri, L. E., Hennig, G. W., Perrino, B. A., Sanders, K. M. (2018). Excitatory Neuronal Responses of Ca2+ Transients in Interstitial Cells of Cajal in the Small Intestine. eNeuro. 5: ENEURO.0080-18.2018.
[23] Jalali-Nezhad, A. A., Frajan-Mahhadi, F., Komeili, G., Barkhordari-Ahmadi, F. (2015) The effect of ginger hydroalcholicextract on rat ileal contraction in vitro. Zahedan J. Res. Med. Sci. 15:29-33.
[24] Kimoto, M., Zeredo J. L., Ota, M. S., Nihei, Z., Toda, K. (2015). Ginger-induced ileal motility is modified by stress: sex differences in rats. J. Food Nutr. Sci. 3:5–8.
[25] Kimoto, M., Zeredo J. L., Ota, M. S., Nihei, Z., Toda, K. (2015). Sansho intake modulates ileum activity in stress-loaded rats. J. Food Nutr. Sci. 3:9–12.
[26] Dickens, E. J., Hirst, G. D. S., Tomita, T. (1999). Identification of rhythmically active cells in guinea-pig stomach. J. Physiol. 514:515–531.
[27] Osa, T. (1973). The inhibitory action of caffeine on the smooth muscles of mouse myometrium and guinea pig ileum. Jpn. J. Physiol. 23:199–216.
[28] Ohta, T., Nakazato, Y. (1993) Chloride currents activated by caffeine in rat intestinal smooth muscle cells. J. Physiol. 465:149-162.
[29] Jin, N. G., Koh, S. D., Sanders, K. M. (2009). Caffeine inhibits nonselective cationic currents in interstitial cells of Cajal from the murine jejunum. Amer. J. Physiol. Cell Physiol. 297:C971–C978.
[30] Meyerhof, W., Batram, C., Kuhn, C., Brockhoff, A., Chudoba E., Bufe, B., Appendino, G., Behrens, M. (2010). The mowwwwwlecular receptive ranges of human TAS2R bitter taste receptors. Chem. Senses 35:157–370.
[31] Kimoto, M., Zeredo, Toda, K. (2012). Hypergravity conditioning on ileal movement in rats. Avit. Space Environ. Med. 83:483–487.
[32] Manson, M. L., Säfholm, J., Al-Ameri, M., Bergman, P., Orre, A. C., Swärd, K., James, A., Dahlén, S. E., Adner, M. (2014). Bitter taste receptor agonists mediate relaxation of human and rodent vascular smooth muscle. Eur. J. Pharmacol. 740:302–11.
[33] Caicedo, A., Pereira, E., Margolskee, R. F., Roper, S. D. (2003). Role of the G-protein subunit alpha-gustducin in taste cell responses to bitter stimuli. J. Neurosci. 23:9947–9952.
[34] Gu, F., Liu, X., Liang, J., Chen, J., Chen, F., Li, F. (2015). Bitter taste receptor mTas2r105 is expressed in small intestinal villus and crypts. Biochem. Biophys. Res. Com. 463:934–941.
[35] Liang, J., Chen, F., Gu, F., Liu, X., Li, F., Du, D. (2017). Expression and functional activity of bitter taste receptors in primary renal tubular epithelial cells and M-1 cells. Mol. Cell. Biochem. 428:193–202.
[36] Wu, S. V., Rozengurt, N., Yang, M., Young, S. H., Sinnett-Smith, J., Rozengurt, E. (2002). Expression of bitter taste receptors of the T2R family in the gastrointestinal tract and enteroendocrine STC-1 cells. Proc. Natl. Acad. Sci. USA. 99:2392–2397.
[37] Kelley, S. P., Walsh, J., Kelly, M. C., Muhdar, S., Adel-Aziz, M., Barrett, I. D., Wildman, S. S. (2014). Inhibition of native 5–HT3 receptor-evoked contractions in guinea pig and mouse ileum by antimalarial drugs, Eur. J. Pharmacol. 738:186–191.
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    Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Mari Kimoto, et al. (2018). Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. International Journal of Nutrition and Food Sciences, 7(3), 94-99. https://doi.org/10.11648/j.ijnfs.20180703.13

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    Atsuko Yamashita; Nana Shimamoto; Kyoko Morita; Hasumi Sugiyama; Mari Kimoto, et al. Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. Int. J. Nutr. Food Sci. 2018, 7(3), 94-99. doi: 10.11648/j.ijnfs.20180703.13

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    AMA Style

    Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Mari Kimoto, et al. Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro. Int J Nutr Food Sci. 2018;7(3):94-99. doi: 10.11648/j.ijnfs.20180703.13

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  • @article{10.11648/j.ijnfs.20180703.13,
      author = {Atsuko Yamashita and Nana Shimamoto and Kyoko Morita and Hasumi Sugiyama and Mari Kimoto and Kazuo Toda and Masato Ota},
      title = {Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {7},
      number = {3},
      pages = {94-99},
      doi = {10.11648/j.ijnfs.20180703.13},
      url = {https://doi.org/10.11648/j.ijnfs.20180703.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijnfs.20180703.13},
      abstract = {Thiamine and quinine are popular bitter substances and their physiological effects have been studied; however, their impact on digestion remains unknown. Here, the physiological effects of thiamine and quinine was investigated for in vitro contraction of mouse ileum. Acetylcholine stimulates autonomous contraction of mouse ileum in a dose-dependent manner. The effect of Acetylcholine for contraction of ileum was partly suppressed by the adrenaline administration. Upon simultaneous treatment of the ileum by acetylcholine, thiamine, and quinine decreased the maximum contraction. The period till half maximum contraction was prolonged by the presence of thiamine and quinine but not by adrenaline. Because a physiological effect of thiamine and quinine was observed on acetylcholine-induced contraction of the ileum, the repertoire of human bitter taste receptors, TAS2R-1, -4, -7, -10, -14, -31, -39, -40, -43, and -46, were investigated to which thiamine and quinine may bind. These human bitter taste receptors were further analyzed among the database for mouse homologs using evolutionally conserved amino acid sequences. The only bitter receptor for both thiamine and quinine was TAS2R-39, the homology of TAS2R-139 to human TAS2R-39 was 74%. Importantly, the homology of mouse TAS2R-119 to human TAS2R-1 which interact with thiamine was 91%, and that of TAS2R-130 to human TAS2R-7 that interact with quinine was 81%. The present study indicated that thiamine and quinine changed the early phase of contraction of ileum in mice and suggested that TAS2R119 and TAS2R130 expressed in mouse enteroendocrine cells to modify the physiological effects of thiamine and quinine on the acetylcholine-induced contraction of the ileum.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Thiamine and Quinine Differently Inhibit the Early Phase of Acetylcholine-Dependent Contraction of Mouse Ileum in vitro
    AU  - Atsuko Yamashita
    AU  - Nana Shimamoto
    AU  - Kyoko Morita
    AU  - Hasumi Sugiyama
    AU  - Mari Kimoto
    AU  - Kazuo Toda
    AU  - Masato Ota
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    PY  - 2018
    N1  - https://doi.org/10.11648/j.ijnfs.20180703.13
    DO  - 10.11648/j.ijnfs.20180703.13
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 94
    EP  - 99
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20180703.13
    AB  - Thiamine and quinine are popular bitter substances and their physiological effects have been studied; however, their impact on digestion remains unknown. Here, the physiological effects of thiamine and quinine was investigated for in vitro contraction of mouse ileum. Acetylcholine stimulates autonomous contraction of mouse ileum in a dose-dependent manner. The effect of Acetylcholine for contraction of ileum was partly suppressed by the adrenaline administration. Upon simultaneous treatment of the ileum by acetylcholine, thiamine, and quinine decreased the maximum contraction. The period till half maximum contraction was prolonged by the presence of thiamine and quinine but not by adrenaline. Because a physiological effect of thiamine and quinine was observed on acetylcholine-induced contraction of the ileum, the repertoire of human bitter taste receptors, TAS2R-1, -4, -7, -10, -14, -31, -39, -40, -43, and -46, were investigated to which thiamine and quinine may bind. These human bitter taste receptors were further analyzed among the database for mouse homologs using evolutionally conserved amino acid sequences. The only bitter receptor for both thiamine and quinine was TAS2R-39, the homology of TAS2R-139 to human TAS2R-39 was 74%. Importantly, the homology of mouse TAS2R-119 to human TAS2R-1 which interact with thiamine was 91%, and that of TAS2R-130 to human TAS2R-7 that interact with quinine was 81%. The present study indicated that thiamine and quinine changed the early phase of contraction of ileum in mice and suggested that TAS2R119 and TAS2R130 expressed in mouse enteroendocrine cells to modify the physiological effects of thiamine and quinine on the acetylcholine-induced contraction of the ileum.
    VL  - 7
    IS  - 3
    ER  - 

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Author Information
  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan

  • Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan

  • Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan

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