American Journal of Life Sciences

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Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia

Received: May 26, 2020    Accepted:     Published: Jun. 29, 2020
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Abstract

Objective: To study the gene and protein expressions of human leukocyte antigen-G (HLA-G) in the placental tissues from healthy women in late pregnancy and pregnant women with preeclampsia (PE), and to explore the relationship between HLA-G and PE. Methods: Immunohistochemistry, Western blot and RT-PCR were used to detect the expressions of HLA-G protein and mRNA in the maternal placenta of 60 pregnant women with PE (including 30 cases of mild PE and 30 cases of severe PE) and 30 healthy full-term pregnant women (control group) respectively. Results: HLA-G had its expression mainly on the surface of trophoblast cells, and its response range and reactivity of its immunologic tissue were further reduced and weakened as the patient's condition was aggravated. The protein expression of placental HLA-G in each PE group was significantly lower than control group, with the difference statistically significant (all P<0.05). Compared with mild PE group, the protein expression level of HLA-G was lower in severe PE group (P<0.05). The Spearman's correlation analysis for HLA-G and PE severity showed that the correlation coefficient was -0.892 (rs = -0.892) (P<0.05), indicating that the expression of HLA-G protein was significantly negatively correlated to the severity of PE. The mRNA expression level of placental HLA-G of each PE group was significantly lower than that of control group, and the difference was statistically significant (all P<0.05). But the mRNA expression level of HLA-G was significantly lower in severe PE group than in mild PE group (P<0.05). Conclusion: The decreased expression of HLA-G in placenta may be involved in the pathogenesis of PE, and can also reflect the severity of this disease.

DOI 10.11648/j.ajls.20200801.13
Published in American Journal of Life Sciences ( Volume 8, Issue 1, February 2020 )
Page(s) 14-18
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

HLA-G, Placenta, Preeclampsia, Pregnancy

References
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[2] Scholten RR, Hopman MT, Sweep FC, et al. Co-occurrence of cardiovascular and prothrombotic risk factors in women with a history of preeclampsia. Obstet Gynecol 2019; 121: 97-105.
[3] Scazzocchio E, Figueras F, Crispi F, et al. Performance of a first-trimester screening of preeclampsia in a routine care low-risk setting. Am J Obstet Gynecol 2019; 208: 203-210.
[4] Warner CM, Lampton PW, Newmark JA, Cohen J. Symposium: innovative techniques in human embryo viability assessment. Soluble human leukocyte antigen-G and pregnancy success. Reprod Biomed Online 2018; 17: 470-485.
[5] Rizzo R, Andersen AS, Lassen MR, et al. Soluble human leukocyte antigen-G isoforms in maternal plasma in early and late pregnancy. Am J Reprod Immunol 2019; 62: 320-338.
[6] Bezerra Maia E Holanda Moura S, Marques Lopes L, Murthi P, da Silva Costa F. Prevention of preeclampsia. J Pregnancy 2017; 15: 90-96.
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[8] Castelli EC, Mendes-Junior CT, Deghaide NH, et al. The genetic structure of 3'untranslated region of the HLA-G gene: polymorphisms and haplotypes. Genes Immun 2019; 11: 134-141.
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[14] Tan CY, Chong YS, Loganath A, et al. Possible gene-gene interaction of KIR2DL4 with its cognate ligand HLA-G in modulating risk for preeclampsia. Reprod Sci 2019; 16: 1135-1143.
[15] Moreau P, Contu L, Alba F, et al. HLA-G gene polymorphism in human placentas: possible association of G*0106 allele with preeclampsia and miscarriage. Biol Reprod 2018; 79: 459-467.
[16] Zhou X, Zhang GY, Wang J, Lu SL, Cao J, Sun LZ. A novel bridge between oxidative stress and immunity: the interaction between hydrogen peroxide and human leukocyte antigen G in placental trophoblasts during preeclampsia. Am J Obstet Gynecol 2018; 206: 7-16.
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    Yongmei Li, Yubin Zhang, Jinfang Wang. (2020). Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia. American Journal of Life Sciences, 8(1), 14-18. https://doi.org/10.11648/j.ajls.20200801.13

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    ACS Style

    Yongmei Li; Yubin Zhang; Jinfang Wang. Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia. Am. J. Life Sci. 2020, 8(1), 14-18. doi: 10.11648/j.ajls.20200801.13

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    AMA Style

    Yongmei Li, Yubin Zhang, Jinfang Wang. Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia. Am J Life Sci. 2020;8(1):14-18. doi: 10.11648/j.ajls.20200801.13

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  • @article{10.11648/j.ajls.20200801.13,
      author = {Yongmei Li and Yubin Zhang and Jinfang Wang},
      title = {Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia},
      journal = {American Journal of Life Sciences},
      volume = {8},
      number = {1},
      pages = {14-18},
      doi = {10.11648/j.ajls.20200801.13},
      url = {https://doi.org/10.11648/j.ajls.20200801.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20200801.13},
      abstract = {Objective: To study the gene and protein expressions of human leukocyte antigen-G (HLA-G) in the placental tissues from healthy women in late pregnancy and pregnant women with preeclampsia (PE), and to explore the relationship between HLA-G and PE. Methods: Immunohistochemistry, Western blot and RT-PCR were used to detect the expressions of HLA-G protein and mRNA in the maternal placenta of 60 pregnant women with PE (including 30 cases of mild PE and 30 cases of severe PE) and 30 healthy full-term pregnant women (control group) respectively. Results: HLA-G had its expression mainly on the surface of trophoblast cells, and its response range and reactivity of its immunologic tissue were further reduced and weakened as the patient's condition was aggravated. The protein expression of placental HLA-G in each PE group was significantly lower than control group, with the difference statistically significant (all P<0.05). Compared with mild PE group, the protein expression level of HLA-G was lower in severe PE group (P<0.05). The Spearman's correlation analysis for HLA-G and PE severity showed that the correlation coefficient was -0.892 (rs = -0.892) (P<0.05), indicating that the expression of HLA-G protein was significantly negatively correlated to the severity of PE. The mRNA expression level of placental HLA-G of each PE group was significantly lower than that of control group, and the difference was statistically significant (all P<0.05). But the mRNA expression level of HLA-G was significantly lower in severe PE group than in mild PE group (P<0.05). Conclusion: The decreased expression of HLA-G in placenta may be involved in the pathogenesis of PE, and can also reflect the severity of this disease.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Expression of Human Leukocyte Antigen-G in Placental Tissues from Pregnant Women with Preeclampsia
    AU  - Yongmei Li
    AU  - Yubin Zhang
    AU  - Jinfang Wang
    Y1  - 2020/06/29
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ajls.20200801.13
    DO  - 10.11648/j.ajls.20200801.13
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 14
    EP  - 18
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20200801.13
    AB  - Objective: To study the gene and protein expressions of human leukocyte antigen-G (HLA-G) in the placental tissues from healthy women in late pregnancy and pregnant women with preeclampsia (PE), and to explore the relationship between HLA-G and PE. Methods: Immunohistochemistry, Western blot and RT-PCR were used to detect the expressions of HLA-G protein and mRNA in the maternal placenta of 60 pregnant women with PE (including 30 cases of mild PE and 30 cases of severe PE) and 30 healthy full-term pregnant women (control group) respectively. Results: HLA-G had its expression mainly on the surface of trophoblast cells, and its response range and reactivity of its immunologic tissue were further reduced and weakened as the patient's condition was aggravated. The protein expression of placental HLA-G in each PE group was significantly lower than control group, with the difference statistically significant (all P<0.05). Compared with mild PE group, the protein expression level of HLA-G was lower in severe PE group (P<0.05). The Spearman's correlation analysis for HLA-G and PE severity showed that the correlation coefficient was -0.892 (rs = -0.892) (P<0.05), indicating that the expression of HLA-G protein was significantly negatively correlated to the severity of PE. The mRNA expression level of placental HLA-G of each PE group was significantly lower than that of control group, and the difference was statistically significant (all P<0.05). But the mRNA expression level of HLA-G was significantly lower in severe PE group than in mild PE group (P<0.05). Conclusion: The decreased expression of HLA-G in placenta may be involved in the pathogenesis of PE, and can also reflect the severity of this disease.
    VL  - 8
    IS  - 1
    ER  - 

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Author Information
  • Department of Gynecology, Binzhou People's Hospital, Binzhou, P.R. China

  • Department of Nuclear Medicine, Binzhou People's Hospital, Binzhou, P.R. China

  • Department of Gynecology, Binzhou People's Hospital, Binzhou, P.R. China

  • Section