American Journal of Life Sciences

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Isolation and Characterization of Lipid-Degrading Bacteria in Wastewater of Food Processing Plants and Restaurants in Can Tho City, Vietnam

Received: Nov. 25, 2014    Accepted: Dec. 05, 2014    Published: Dec. 31, 2014
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Abstract

High lipid (fats and oils) concentration contained in wastewater inhibits the activity of microbes in biological wastewater treatment systems. The lipids degradation capability of lipid-degrading bacteria was investigated for possible application in treatment of lipids-contaminated wastewater. One hundred and two bacterial isolates were isolated from 43 vegetable oil- contaminated wastewater samples of many food processing plants and restaurants in 5 districts of Can Tho city, Vietnam on LB medium. There were sixty-one isolates produced clear zones on Tw20 medium, only eleven of which were found to have the high ability to degrade vegetable oil in the contaminated wastewater. These eleven isolates were identified by PCR technique and DNA sequencing. The results of DNA sequencing were compared with GenBank database of NCBI by BLAT N software. The sequences from selected isolates showed high degrees of similarity to those of the GenBank references (between 97% and 99%). Two isolates belonged to Bacilli (18.18%) and nine isolates belonged to Gammaproteobacteria (81.82%). Based on Pi value (nucleotide diversity), Gammaproteobacteria group had the highest Theta values. Theta value (per sequence) from S of SNP for DNA polymorphism were calculated for each group and 11 strains of lipid-degrading bacteria had high genetic diversity. The results propose Acinetobacteria soli strain AL3 a potential bioproduct for wastewater treatment because of its high ability of lipid degradation and biosafety.

DOI 10.11648/j.ajls.20140206.18
Published in American Journal of Life Sciences ( Volume 2, Issue 6, December 2014 )
Page(s) 382-388
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Acinetobacteria, Bacilli, Food Processing Plants and Restaurants, Lipid Contaminated Wastewater, Lipid Degradation, Vegetable Oil

References
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    Ngo Thanh Phong, Nguyen Thanh Duyen, Cao Ngoc Diep. (2014). Isolation and Characterization of Lipid-Degrading Bacteria in Wastewater of Food Processing Plants and Restaurants in Can Tho City, Vietnam. American Journal of Life Sciences, 2(6), 382-388. https://doi.org/10.11648/j.ajls.20140206.18

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    ACS Style

    Ngo Thanh Phong; Nguyen Thanh Duyen; Cao Ngoc Diep. Isolation and Characterization of Lipid-Degrading Bacteria in Wastewater of Food Processing Plants and Restaurants in Can Tho City, Vietnam. Am. J. Life Sci. 2014, 2(6), 382-388. doi: 10.11648/j.ajls.20140206.18

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    AMA Style

    Ngo Thanh Phong, Nguyen Thanh Duyen, Cao Ngoc Diep. Isolation and Characterization of Lipid-Degrading Bacteria in Wastewater of Food Processing Plants and Restaurants in Can Tho City, Vietnam. Am J Life Sci. 2014;2(6):382-388. doi: 10.11648/j.ajls.20140206.18

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  • @article{10.11648/j.ajls.20140206.18,
      author = {Ngo Thanh Phong and Nguyen Thanh Duyen and Cao Ngoc Diep},
      title = {Isolation and Characterization of Lipid-Degrading Bacteria in Wastewater of Food Processing Plants and Restaurants in Can Tho City, Vietnam},
      journal = {American Journal of Life Sciences},
      volume = {2},
      number = {6},
      pages = {382-388},
      doi = {10.11648/j.ajls.20140206.18},
      url = {https://doi.org/10.11648/j.ajls.20140206.18},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20140206.18},
      abstract = {High lipid (fats and oils) concentration contained in wastewater inhibits the activity of microbes in biological wastewater treatment systems. The lipids degradation capability of lipid-degrading bacteria was investigated for possible application in treatment of lipids-contaminated wastewater. One hundred and two bacterial isolates were isolated from 43 vegetable oil- contaminated wastewater samples of many food processing plants and restaurants in 5 districts of Can Tho city, Vietnam on LB medium. There were sixty-one isolates produced clear zones on Tw20 medium, only eleven of which were found to have the high ability to degrade vegetable oil in the contaminated wastewater. These eleven isolates were identified by PCR technique and DNA sequencing. The results of DNA sequencing were compared with GenBank database of NCBI by BLAT N software. The sequences from selected isolates showed high degrees of similarity to those of the GenBank references (between 97% and 99%). Two isolates belonged to Bacilli (18.18%) and nine isolates belonged to Gammaproteobacteria (81.82%). Based on Pi value (nucleotide diversity), Gammaproteobacteria group had the highest Theta values. Theta value (per sequence) from S of SNP for DNA polymorphism were calculated for each group and 11 strains of lipid-degrading bacteria had high genetic diversity. The results propose Acinetobacteria soli strain AL3 a potential bioproduct for wastewater treatment because of its high ability of lipid degradation and biosafety.},
     year = {2014}
    }
    

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    T1  - Isolation and Characterization of Lipid-Degrading Bacteria in Wastewater of Food Processing Plants and Restaurants in Can Tho City, Vietnam
    AU  - Ngo Thanh Phong
    AU  - Nguyen Thanh Duyen
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    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
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    UR  - https://doi.org/10.11648/j.ajls.20140206.18
    AB  - High lipid (fats and oils) concentration contained in wastewater inhibits the activity of microbes in biological wastewater treatment systems. The lipids degradation capability of lipid-degrading bacteria was investigated for possible application in treatment of lipids-contaminated wastewater. One hundred and two bacterial isolates were isolated from 43 vegetable oil- contaminated wastewater samples of many food processing plants and restaurants in 5 districts of Can Tho city, Vietnam on LB medium. There were sixty-one isolates produced clear zones on Tw20 medium, only eleven of which were found to have the high ability to degrade vegetable oil in the contaminated wastewater. These eleven isolates were identified by PCR technique and DNA sequencing. The results of DNA sequencing were compared with GenBank database of NCBI by BLAT N software. The sequences from selected isolates showed high degrees of similarity to those of the GenBank references (between 97% and 99%). Two isolates belonged to Bacilli (18.18%) and nine isolates belonged to Gammaproteobacteria (81.82%). Based on Pi value (nucleotide diversity), Gammaproteobacteria group had the highest Theta values. Theta value (per sequence) from S of SNP for DNA polymorphism were calculated for each group and 11 strains of lipid-degrading bacteria had high genetic diversity. The results propose Acinetobacteria soli strain AL3 a potential bioproduct for wastewater treatment because of its high ability of lipid degradation and biosafety.
    VL  - 2
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    ER  - 

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Author Information
  • Dept. of Biology, College of Natural Sciences, Can Tho University, Can Tho City, Vietnam

  • Center for Continuing Education of Cairang district, Can Tho city, Vietnam

  • Dept. Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam

  • Section