American Journal of Life Sciences

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Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.

Received: Nov. 07, 2014    Accepted: Nov. 15, 2014    Published: Nov. 20, 2014
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Abstract

A bacterium was isolated from the natural source. Gram staining & spore staining showed that the organism is gram positive and forms spore during adverse condition in the growth medium. After various tests it was suggested and the features agreed with the description of Bacillus sp in Bergey’s Manual of Systematic Bacteriology [24]. It was also identified as Bacillus sp with 99.9% identity by API 50 CHB. In growth curve determination showed that the growth of the organism is increased with the increase of incubation period and the growth reached maximum at around 24 hours of incubation and the protease activity was the maximum of the 26 hours culture. This microbe has grown at high temperature and pressure. Its optimum pH and temperature were 8.5 and 60°C. It secretes an extracellular protease in the growth medium. The enzyme hydrolyses a number of proteins including azocasein which suggests that that it is an extracellular protease. The enzyme seems to be alkaline protease which is capable of De-Hairing from skin and hides. A number of companies such as NOVO chemicals started to produce NOVOzymes for tannery industries. The potential for use of microbial enzymes in leather processing lies mainly in areas in which pollution-causing chemicals are being used.

DOI 10.11648/j.ajls.20140206.12
Published in American Journal of Life Sciences ( Volume 2, Issue 6, December 2014 )
Page(s) 338-344
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Identification, Characterization of Protease, Bacillus sp., Isolation, Alkaline Protease

References
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[7] El-Refai, M.H.; Fatah,A.F.A. (2005). Improvement of the newly isolated Bacillus pumilus FH9 Keratinolytic activity. Process Biochem., 40, 2325-2332.
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  • APA Style

    Md. Ekhlas Uddin, Pulak Maitra, Hossain Md. Faruquee, Md. Firoz Alam. (2014). Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.. American Journal of Life Sciences, 2(6), 338-344. https://doi.org/10.11648/j.ajls.20140206.12

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    ACS Style

    Md. Ekhlas Uddin; Pulak Maitra; Hossain Md. Faruquee; Md. Firoz Alam. Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.. Am. J. Life Sci. 2014, 2(6), 338-344. doi: 10.11648/j.ajls.20140206.12

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    AMA Style

    Md. Ekhlas Uddin, Pulak Maitra, Hossain Md. Faruquee, Md. Firoz Alam. Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.. Am J Life Sci. 2014;2(6):338-344. doi: 10.11648/j.ajls.20140206.12

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  • @article{10.11648/j.ajls.20140206.12,
      author = {Md. Ekhlas Uddin and Pulak Maitra and Hossain Md. Faruquee and Md. Firoz Alam},
      title = {Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.},
      journal = {American Journal of Life Sciences},
      volume = {2},
      number = {6},
      pages = {338-344},
      doi = {10.11648/j.ajls.20140206.12},
      url = {https://doi.org/10.11648/j.ajls.20140206.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajls.20140206.12},
      abstract = {A bacterium was isolated from the natural source. Gram staining & spore staining showed that the organism is gram positive and forms spore during adverse condition in the growth medium. After various tests it was suggested and the features agreed with the description of Bacillus sp in Bergey’s Manual of Systematic Bacteriology [24]. It was also identified as Bacillus sp with 99.9% identity by API 50 CHB. In growth curve determination showed that the growth of the organism is increased with the increase of incubation period and the growth reached maximum at around 24 hours of incubation and the protease activity was the maximum of the 26 hours culture. This microbe has grown at high temperature and pressure. Its optimum pH and temperature were 8.5 and 60°C. It secretes an extracellular protease in the growth medium. The enzyme hydrolyses a number of proteins including azocasein which suggests that that it is an extracellular protease. The enzyme seems to be alkaline protease which is capable of De-Hairing from skin and hides. A number of companies such as NOVO chemicals started to produce NOVOzymes for tannery industries. The potential for use of microbial enzymes in leather processing lies mainly in areas in which pollution-causing chemicals are being used.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.
    AU  - Md. Ekhlas Uddin
    AU  - Pulak Maitra
    AU  - Hossain Md. Faruquee
    AU  - Md. Firoz Alam
    Y1  - 2014/11/20
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ajls.20140206.12
    DO  - 10.11648/j.ajls.20140206.12
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 338
    EP  - 344
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20140206.12
    AB  - A bacterium was isolated from the natural source. Gram staining & spore staining showed that the organism is gram positive and forms spore during adverse condition in the growth medium. After various tests it was suggested and the features agreed with the description of Bacillus sp in Bergey’s Manual of Systematic Bacteriology [24]. It was also identified as Bacillus sp with 99.9% identity by API 50 CHB. In growth curve determination showed that the growth of the organism is increased with the increase of incubation period and the growth reached maximum at around 24 hours of incubation and the protease activity was the maximum of the 26 hours culture. This microbe has grown at high temperature and pressure. Its optimum pH and temperature were 8.5 and 60°C. It secretes an extracellular protease in the growth medium. The enzyme hydrolyses a number of proteins including azocasein which suggests that that it is an extracellular protease. The enzyme seems to be alkaline protease which is capable of De-Hairing from skin and hides. A number of companies such as NOVO chemicals started to produce NOVOzymes for tannery industries. The potential for use of microbial enzymes in leather processing lies mainly in areas in which pollution-causing chemicals are being used.
    VL  - 2
    IS  - 6
    ER  - 

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Author Information
  • Dept. Of Biotechnology & Genetic Engineering, Islamic University, Kushtia, Bangladesh

  • Dept. Of Biotechnology & Genetic Engineering, Islamic University, Kushtia, Bangladesh

  • Dept. Of Biotechnology & Genetic Engineering, Islamic University, Kushtia, Bangladesh; Vaccine research Laboratory, Gono University, Savar, Dhaka

  • Dept. Of Biotechnology & Genetic Engineering, Islamic University, Kushtia, Bangladesh

  • Section