European Journal of Biophysics

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Molecular Phylogenetic Study on Pseudomonas stutzeri Isolated from Currency Notes in Khartoum State, Sudan and Identified Via16s rRNA Gene Sequence Analysis

Received: Nov. 28, 2017    Accepted: Dec. 19, 2017    Published: Jan. 17, 2018
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Abstract

Pseudomonas stutzeri is a valuable bacteria for understanding of the taxonomical and the phylogenetic relationships. The study of genetic relationships between organisms or genes is carried out by molecular phylogeny. We aim to study the relationships according to16S rRNA gene sequences between our samples and the closest strains from other countries over the world. To our knowledge, the phylogenetic studies between strains from Sudan with strains from other countries were not done before. A total of 140 currency notes in different denominations were collected randomly from several locations including hospitals, food sellers and transporters. From the collected notes, a total of 135 bacterial colonies were isolated and from them 14 isolates were identified as a Pseudomonas stutzeri. In the study, streaking plate method was used for the isolation of pure bacterial culture, Chelex 100 method was used for DNA extraction, conventional PCR was used for amplification of the targeted gene, agarose gel electrophoresis and various bioinformatics tools were used for nucleotide sequence analysis. The PCR products were sent for Macrogen Company-Netherlands for purification and nucleotide sequencing. After sequencing 3 samples were noisy, hence they were excluded. According to phylogenetic analysis, we found that the samples were closely related to strains from south-east Asia (Indonesia), east Asia (China), south-central Asia (Bangladesh), south Asia (India), north Africa (Tunisia) and south Europe (Italy and Greece). Despite the samples were from the same source (currency notes), we found that there is broad sequence variation between them.

DOI 10.11648/j.ejb.20170506.12
Published in European Journal of Biophysics ( Volume 5, Issue 6, December 2017 )
Page(s) 104-111
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Pseudomonas stutzeri, Currency Notes, Molecular Phylogenetic, Khartoum, Sudan

References
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  • APA Style

    Mosab Yahya Al-Nour, Malik Suliman Mohamed, Noha Ahmed Abd Alfadil, Hisham Nouraldayem Altayb, Salah-Eldin Gumaa El-lzaki, et al. (2018). Molecular Phylogenetic Study on Pseudomonas stutzeri Isolated from Currency Notes in Khartoum State, Sudan and Identified Via16s rRNA Gene Sequence Analysis. European Journal of Biophysics, 5(6), 104-111. https://doi.org/10.11648/j.ejb.20170506.12

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    ACS Style

    Mosab Yahya Al-Nour; Malik Suliman Mohamed; Noha Ahmed Abd Alfadil; Hisham Nouraldayem Altayb; Salah-Eldin Gumaa El-lzaki, et al. Molecular Phylogenetic Study on Pseudomonas stutzeri Isolated from Currency Notes in Khartoum State, Sudan and Identified Via16s rRNA Gene Sequence Analysis. Eur. J. Biophys. 2018, 5(6), 104-111. doi: 10.11648/j.ejb.20170506.12

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    AMA Style

    Mosab Yahya Al-Nour, Malik Suliman Mohamed, Noha Ahmed Abd Alfadil, Hisham Nouraldayem Altayb, Salah-Eldin Gumaa El-lzaki, et al. Molecular Phylogenetic Study on Pseudomonas stutzeri Isolated from Currency Notes in Khartoum State, Sudan and Identified Via16s rRNA Gene Sequence Analysis. Eur J Biophys. 2018;5(6):104-111. doi: 10.11648/j.ejb.20170506.12

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  • @article{10.11648/j.ejb.20170506.12,
      author = {Mosab Yahya Al-Nour and Malik Suliman Mohamed and Noha Ahmed Abd Alfadil and Hisham Nouraldayem Altayb and Salah-Eldin Gumaa El-lzaki and Mohamed Ahmed Hassan},
      title = {Molecular Phylogenetic Study on Pseudomonas stutzeri Isolated from Currency Notes in Khartoum State, Sudan and Identified Via16s rRNA Gene Sequence Analysis},
      journal = {European Journal of Biophysics},
      volume = {5},
      number = {6},
      pages = {104-111},
      doi = {10.11648/j.ejb.20170506.12},
      url = {https://doi.org/10.11648/j.ejb.20170506.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ejb.20170506.12},
      abstract = {Pseudomonas stutzeri is a valuable bacteria for understanding of the taxonomical and the phylogenetic relationships. The study of genetic relationships between organisms or genes is carried out by molecular phylogeny. We aim to study the relationships according to16S rRNA gene sequences between our samples and the closest strains from other countries over the world. To our knowledge, the phylogenetic studies between strains from Sudan with strains from other countries were not done before. A total of 140 currency notes in different denominations were collected randomly from several locations including hospitals, food sellers and transporters. From the collected notes, a total of 135 bacterial colonies were isolated and from them 14 isolates were identified as a Pseudomonas stutzeri. In the study, streaking plate method was used for the isolation of pure bacterial culture, Chelex 100 method was used for DNA extraction, conventional PCR was used for amplification of the targeted gene, agarose gel electrophoresis and various bioinformatics tools were used for nucleotide sequence analysis. The PCR products were sent for Macrogen Company-Netherlands for purification and nucleotide sequencing. After sequencing 3 samples were noisy, hence they were excluded. According to phylogenetic analysis, we found that the samples were closely related to strains from south-east Asia (Indonesia), east Asia (China), south-central Asia (Bangladesh), south Asia (India), north Africa (Tunisia) and south Europe (Italy and Greece). Despite the samples were from the same source (currency notes), we found that there is broad sequence variation between them.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Molecular Phylogenetic Study on Pseudomonas stutzeri Isolated from Currency Notes in Khartoum State, Sudan and Identified Via16s rRNA Gene Sequence Analysis
    AU  - Mosab Yahya Al-Nour
    AU  - Malik Suliman Mohamed
    AU  - Noha Ahmed Abd Alfadil
    AU  - Hisham Nouraldayem Altayb
    AU  - Salah-Eldin Gumaa El-lzaki
    AU  - Mohamed Ahmed Hassan
    Y1  - 2018/01/17
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ejb.20170506.12
    DO  - 10.11648/j.ejb.20170506.12
    T2  - European Journal of Biophysics
    JF  - European Journal of Biophysics
    JO  - European Journal of Biophysics
    SP  - 104
    EP  - 111
    PB  - Science Publishing Group
    SN  - 2329-1737
    UR  - https://doi.org/10.11648/j.ejb.20170506.12
    AB  - Pseudomonas stutzeri is a valuable bacteria for understanding of the taxonomical and the phylogenetic relationships. The study of genetic relationships between organisms or genes is carried out by molecular phylogeny. We aim to study the relationships according to16S rRNA gene sequences between our samples and the closest strains from other countries over the world. To our knowledge, the phylogenetic studies between strains from Sudan with strains from other countries were not done before. A total of 140 currency notes in different denominations were collected randomly from several locations including hospitals, food sellers and transporters. From the collected notes, a total of 135 bacterial colonies were isolated and from them 14 isolates were identified as a Pseudomonas stutzeri. In the study, streaking plate method was used for the isolation of pure bacterial culture, Chelex 100 method was used for DNA extraction, conventional PCR was used for amplification of the targeted gene, agarose gel electrophoresis and various bioinformatics tools were used for nucleotide sequence analysis. The PCR products were sent for Macrogen Company-Netherlands for purification and nucleotide sequencing. After sequencing 3 samples were noisy, hence they were excluded. According to phylogenetic analysis, we found that the samples were closely related to strains from south-east Asia (Indonesia), east Asia (China), south-central Asia (Bangladesh), south Asia (India), north Africa (Tunisia) and south Europe (Italy and Greece). Despite the samples were from the same source (currency notes), we found that there is broad sequence variation between them.
    VL  - 5
    IS  - 6
    ER  - 

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Author Information
  • Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Omdurman Islamic University, Khartoum, Sudan

  • Department of Pharmaceutics, Faculty of Pharmacy, University of Khartoum, Khartoum, Sudan

  • Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Alneelain, Khartoum, Sudan

  • Department of Microbiology, College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan

  • Department of Epidemiology, Molecular Epidemiology Laboratory, Tropical Medicine Institute Research, Khartoum, Sudan

  • Applied Bioinformatics Center, Africa City of Technology, Khartoum, Sudan

  • Section