The study had aimed to characterize the production of hydrogen gases by anaerobic bacteria. One isolate was found in sheep ruminal fluid and four isolates were obtained from the activated sludge. These isolates were identified by microscopic methods and by rRNA sequences. One ruminal bacterium was identified as Escherichia coli, and it was found that these isolates from activated sludge were related to Clostridium botulinum, C. perfringens and C. difficile. One strain could not be assigned to any species but was similar to C. botulinum. Growth and production of the metabolic gases with molasses as sole carbon source were measured during the anaerobic cultivation by Micro-Oxymax (Columbus Instruments, Columbus, OH, U.S.A.) gas analyzer. One of the most available saccharidic waste products is molasses. The growth on molasses as carbon source was done to test the production of H2. It was found that all tested Clostridium isolates (AK 1-4, AK 1-5, AK 1-9 and AK 1-12) and E. coli isolate (No 2- 24) had utilized molasses as carbon source monitored by production of CO2 gas. All these strains produced H2 gas, and CO gas in concentration range 102 μmol L–1, and H2S gas in concentrations lower by one order of magnitude. Kinetics of evolution of these gases was different suggesting that they are produced by independent processes. Results show that metabolic gases are produced mainly in the exponential phase of growth.
| DOI | 10.11648/j.ab.20170506.11 |
| Published in | Advances in Biochemistry (Volume 5, Issue 6, December 2017) |
| Page(s) | 110-116 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Clostridium Spp., E. Coli, Molasses, Hydrogen, Metabolic Gases, Anaerobic Metabolism
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APA Style
Rasha Jame, Boris Lakatoš, Mawia Hassan, Mutasim Elhag, Ludovit Varečka. (2017). Production of Bio-Hydrogen Gas and Other Metabolic Gases by Anaerobic Bacteria Grown on Molasses. Advances in Biochemistry, 5(6), 110-116. https://doi.org/10.11648/j.ab.20170506.11
ACS Style
Rasha Jame; Boris Lakatoš; Mawia Hassan; Mutasim Elhag; Ludovit Varečka. Production of Bio-Hydrogen Gas and Other Metabolic Gases by Anaerobic Bacteria Grown on Molasses. Adv. Biochem. 2017, 5(6), 110-116. doi: 10.11648/j.ab.20170506.11
AMA Style
Rasha Jame, Boris Lakatoš, Mawia Hassan, Mutasim Elhag, Ludovit Varečka. Production of Bio-Hydrogen Gas and Other Metabolic Gases by Anaerobic Bacteria Grown on Molasses. Adv Biochem. 2017;5(6):110-116. doi: 10.11648/j.ab.20170506.11
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Download@article{10.11648/j.ab.20170506.11,
author = {Rasha Jame and Boris Lakatoš and Mawia Hassan and Mutasim Elhag and Ludovit Varečka},
title = {Production of Bio-Hydrogen Gas and Other Metabolic Gases by Anaerobic Bacteria Grown on Molasses},
journal = {Advances in Biochemistry},
volume = {5},
number = {6},
pages = {110-116},
doi = {10.11648/j.ab.20170506.11},
url = {https://doi.org/10.11648/j.ab.20170506.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20170506.11},
abstract = {The study had aimed to characterize the production of hydrogen gases by anaerobic bacteria. One isolate was found in sheep ruminal fluid and four isolates were obtained from the activated sludge. These isolates were identified by microscopic methods and by rRNA sequences. One ruminal bacterium was identified as Escherichia coli, and it was found that these isolates from activated sludge were related to Clostridium botulinum, C. perfringens and C. difficile. One strain could not be assigned to any species but was similar to C. botulinum. Growth and production of the metabolic gases with molasses as sole carbon source were measured during the anaerobic cultivation by Micro-Oxymax (Columbus Instruments, Columbus, OH, U.S.A.) gas analyzer. One of the most available saccharidic waste products is molasses. The growth on molasses as carbon source was done to test the production of H2. It was found that all tested Clostridium isolates (AK 1-4, AK 1-5, AK 1-9 and AK 1-12) and E. coli isolate (No 2- 24) had utilized molasses as carbon source monitored by production of CO2 gas. All these strains produced H2 gas, and CO gas in concentration range 102 μmol L–1, and H2S gas in concentrations lower by one order of magnitude. Kinetics of evolution of these gases was different suggesting that they are produced by independent processes. Results show that metabolic gases are produced mainly in the exponential phase of growth.},
year = {2017}
}
TY - JOUR T1 - Production of Bio-Hydrogen Gas and Other Metabolic Gases by Anaerobic Bacteria Grown on Molasses AU - Rasha Jame AU - Boris Lakatoš AU - Mawia Hassan AU - Mutasim Elhag AU - Ludovit Varečka Y1 - 2017/11/22 PY - 2017 N1 - https://doi.org/10.11648/j.ab.20170506.11 DO - 10.11648/j.ab.20170506.11 T2 - Advances in Biochemistry JF - Advances in Biochemistry JO - Advances in Biochemistry SP - 110 EP - 116 PB - Science Publishing Group SN - 2329-0862 UR - https://doi.org/10.11648/j.ab.20170506.11 AB - The study had aimed to characterize the production of hydrogen gases by anaerobic bacteria. One isolate was found in sheep ruminal fluid and four isolates were obtained from the activated sludge. These isolates were identified by microscopic methods and by rRNA sequences. One ruminal bacterium was identified as Escherichia coli, and it was found that these isolates from activated sludge were related to Clostridium botulinum, C. perfringens and C. difficile. One strain could not be assigned to any species but was similar to C. botulinum. Growth and production of the metabolic gases with molasses as sole carbon source were measured during the anaerobic cultivation by Micro-Oxymax (Columbus Instruments, Columbus, OH, U.S.A.) gas analyzer. One of the most available saccharidic waste products is molasses. The growth on molasses as carbon source was done to test the production of H2. It was found that all tested Clostridium isolates (AK 1-4, AK 1-5, AK 1-9 and AK 1-12) and E. coli isolate (No 2- 24) had utilized molasses as carbon source monitored by production of CO2 gas. All these strains produced H2 gas, and CO gas in concentration range 102 μmol L–1, and H2S gas in concentrations lower by one order of magnitude. Kinetics of evolution of these gases was different suggesting that they are produced by independent processes. Results show that metabolic gases are produced mainly in the exponential phase of growth. VL - 5 IS - 6 ER -
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