Introduction: Tuberculosis (TB) has the potential to be a serious health problem in Sudan. Early laboratory confirmation of TB can lead to early initiation of treatment and increased opportunities to interrupt transmission. Nucleic acid amplification tests are widely used for detection and species differentiation of Mycobacteria in clinical specimens. This study aimed to detect specific members of Mycobacterium tuberculosis complex in lymphocytic pleural effusion. 88 pleural effusions were cytologically examined, of which 52 were diagnosed as showing chronic inflammatory reactions. The pleural effusions were from Sudanese patients clinically suspected to have pulmonary tuberculosis. DNA was isolated using DNA extraction kits from Vivants (Malaysia). PCR Amplification of the target sequence (IS-1081 gene, 344 bp) was carried out and then analyzed using agarose gel electrophoresis. Out of the 52 studied samples of lymphocytic pleural effusions, 22 (50%) of the DNA positive samples were positive for IS-1081 gene. Molecular assays of Mycobacterium tuberculosis complex can provide rapid diagnosis of the tuberculosis which is one of the major causes of pleural effusions in general and lymphocytic effusions in specific in Sudan.
| Published in | Pathology and Laboratory Medicine (Volume 10, Issue 1) |
| DOI | 10.11648/j.plm.20261001.11 |
| Page(s) | 1-4 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2026. Published by Science Publishing Group |
Tuberculosis, TB, Mycobacterium Tuberculosis Complex, PCR, IS-1081 Gene
| [1] |
Lee, H.; Kim, J.; Kim, J. and Park, Y. (2025). Review of the Global Burden of Tuberculosis in 2023: Insights from the WHO Global Tuberculosis Report 2024. Public Health Weekly Report 2025; 18(Suppl 11): S55-S69.
https://doi.org/10.56786/PHWR.2025.18.11suppl.5 Epub 2025 Mar 5. |
| [2] | Ahmed Khan, J; Irfan, M; Zaki, A; Beg, M.; Hussain, S. F.; Rizvi, N. (2006). Knowledge, attitude and misconceptions regarding tuberculosis in Pakistan patients. Journal of Pakistan medical association, 56(5): 211-214. |
| [3] | Zhai, K.; Lu, Y. and Shi, H. (2016). Tuberculous pleural effusion. Journal of thoracic diseases, 2016, 8(7): E486-E494. |
| [4] | Khan, F. Y.; Alsamawi, M.; Yasin, M.; Ibrahim, A. S.; Hamza, M.; Lingawi, M.; Abbas, M. T.; Musa R. M. (2011). Etiology of pleural effusion among adults in the State of Qatar: a 1-year hospital-based study. Eastern Mediterranean Health Journal (EMHJ) 17(7): 611-618. |
| [5] | Pandey, S. M. and Mishra, A. K. (2025) 'Role of Thoracoscopy in Establishing Diagnosis of Tuberculosis as a Cause of Pleural Effusion in a TB Endemic Area' International Journal of Medical and Pharmaceutical Research 6(5), pp. 1811-1816. |
| [6] | Vorster M. J.; Allwood B. W.; Diacon, A. H.; Koegelenberg, C. F.(2015). Tuberculous pleural effusions: advances and controversies. J Thorac Dis. 2015 Jun; 7(6): 981-91. |
| [7] | Vidal, R.; de Gracia, J.; Ruiz, J.; Fite, E.; Monsó, E.; Martín, N. (1986). Controlled study of 637 patients with tuberculosis. Diagnosis and therapeutic results with 9- and 6-month regimens]. Med Clin (Barc). 87(9): 368-70. |
| [8] | Liu, S.; Yang, Y.; Wang, D.; Gao, L.; J Qin, J.; Wu, J.; Li, D.; Li, X.; Chen, M.; Wang, H.; Shen, Y.; Wen, F. and Chen, F. (2025). Development and validation of a prediction model based on a nomogram for tuberculous pleural effusion. Front. Med. 12: 1589406. |
| [9] | McNally, E.; Ross, C. and Gleeson, L. E. (2023). The tuberculosis pleural effusion. Breathe 19(4): 230143; |
| [10] | Chang, F. (2007). Clinical Utility of Polymerase Chain Reaction for the Diagnosis of Smear-negative Pleural Tuberculosis, J Chin Med Assoc. 2007, 70(4): 146-147. |
| [11] | Reechaipichitkul, W.; Lulitanond, V.; Sungkeeree, S.; Patjanasoontorn, B. (2000). Rapid diagnosis of tuberculous pleural effusion using polymerase chain reaction. SJTMAK, 2000, 31(3): 509-514. |
| [12] | Green, C.; Huggett, J. F.; Talbot, E.; Mwaba, P.; Reither, K.; Zumla A. I. (2009). Rapid diagnosis of tuberculosis through the detection of mycobacterial DNA in urine by nucleic acid amplification methods. Lancet Infect Dis. 9(8): 505-511. |
| [13] | Zaki, A. M.; Ibrahim, N. Y.; Abdelsalam, A. M.; Osman, M. M. (2011). A study on Prevalence of Drug Resistance in Drug Default Pulmonary Tuberculosis. Sudan JMS 6(2): 97-101. |
| [14] | Rozales F. P.; Machado A. B.; DE Paris F.; Zavascki A. P.; Barth A. L. (2013). PCR to detect Mycobacterium tuberculosis in respiratory tract samples: evaluation of clinical data. Epidemiol Infect. 10: 1-7. |
| [15] | Gopi, A.; Madhavan, S. M.; Sharma, S. K.; Sahn, S. A. (2007). Diagnosis and treatment of tuberculous pleural effusion in 2006. Chest. 131(3): 880-889. |
| [16] | Fatolahzadeh, P.; Maleknejad, A.; Bahador, Peeri- Dogaheh, H. Alikhani, M. Y.; Radmanesh-Ahsani, R. (2007). Evaluation of Different Primer Sets for the Rapid Diagnosis of Tuberculosis. Pakistan Journal of Biological Sciences, 10: 107-111. |
| [17] |
Riojas, M. A.; McGough K. J.; Rider-Riojas C. J.; Rastogi, N.; Hazbón, M. H. (2018). Phylogenomic analysis of the species of the Mycobacterium tuberculosis complex demonstrates that Mycobacterium africanum, Mycobacterium bovis, Mycobacterium caprae, Mycobacterium microti and Mycobacterium pinnipedii are later heterotypic synonyms of Mycobacterium tuberculosis. Int J Syst Evol Microbiol. 2018 Jan; 68(1): 324-332.
https://doi.org/10.1099/ijsem.0.002507 Epub 2017 Dec 5. |
| [18] | Leopold, G.; Koss.; Myron, R.; Melamed. (2006). Koss' diagnostic cytology and its histopathologic bases, Vol. 1, 5th ed. Lippincott Williams & Wilkins Publishers, Philadelphia: USA. |
| [19] | WHO (2024). Tuberculosis resurges as top infectious disease killer, WHO Global Tuberculosis Report 2024, 29 October. Available at: World Health Organization Global TB Report 2024. |
| [20] | WHO (2010). The human face of tuberculosis in Sudan. World Health Organization. Regional Office for the eastern Mediterranean. |
| [21] | Smith, I. (2003). Mycobacterium tuberculosis Pathogenesis and Molecular Determinants of Virulence Clin Microbiol Rev. 2003 July; 16(3): 463–496. |
| [22] | Ledesma, J. R. et al. (2019). Global, regional, and national sex differences in the global burden of tuberculosis by HIV status, 1990–2019: results from the Global Burden of Disease Study 2019. The Lancet. 22(2) p 222-241. |
| [23] | Valdés, L.; Alvarez, D.; Valle, J. M.; Pose, A.; San José, E. (1996). The etiology of pleural effusions in an area with high incidence of tuberculosis. Chest. 109(1): 158-62. |
| [24] | Abdelaziz, A. A., El-Gamal, E. A., Rezk, N. A., Abdalla, D. A., & El-Gamal, E. (2024). Validity of pleuroscopy in evaluating pleural effusions of uncertain etiology at the Cardiothoracic Unit of Minia University Hospital, Egypt. Egyptian journal of chest diseases and tuberculosis. Cureus, 29, 16(4): e59300. |
| [25] | Elmadhoun, W. M.; Noor, S. K.; Bushara S. O.; Ahmed, E. O.; Suliman, A. A. et al. (2016). Epidemiology of tuberculosis and evaluation of treatment outcomes in the national tuberculosis control programme, River Nile state, Sudan, 2011-2013. EMHJ. 22(2): 95-102. |
APA Style
Eltahir, H. B., Hosaien, M. K., Musa, H. E. O., Mohammed, R. Y. H., Elmubarak, A. O., et al. (2026). PCR IS--1081 Gene-based Detection of Mycobacterium Tuberculosis Complex in lymphocytic Pleural Effusion Samples of Sudanese Patients. Pathology and Laboratory Medicine, 10(1), 1-4. https://doi.org/10.11648/j.plm.20261001.11
ACS Style
Eltahir, H. B.; Hosaien, M. K.; Musa, H. E. O.; Mohammed, R. Y. H.; Elmubarak, A. O., et al. PCR IS--1081 Gene-based Detection of Mycobacterium Tuberculosis Complex in lymphocytic Pleural Effusion Samples of Sudanese Patients. Pathol. Lab. Med. 2026, 10(1), 1-4. doi: 10.11648/j.plm.20261001.11
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Download@article{10.11648/j.plm.20261001.11,
author = {Hanan Babiker Eltahir and Mona Kamal Hosaien and Hind Elrayah Osman Musa and Ramy Yousif Hasab-Elrasul Mohammed and Ahmed Omar Elmubarak and Nazik Elmalaika Obaid Seid Ahmed Husain},
title = {PCR IS--1081 Gene-based Detection of Mycobacterium Tuberculosis Complex in lymphocytic Pleural Effusion Samples of Sudanese Patients},
journal = {Pathology and Laboratory Medicine},
volume = {10},
number = {1},
pages = {1-4},
doi = {10.11648/j.plm.20261001.11},
url = {https://doi.org/10.11648/j.plm.20261001.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plm.20261001.11},
abstract = {Introduction: Tuberculosis (TB) has the potential to be a serious health problem in Sudan. Early laboratory confirmation of TB can lead to early initiation of treatment and increased opportunities to interrupt transmission. Nucleic acid amplification tests are widely used for detection and species differentiation of Mycobacteria in clinical specimens. This study aimed to detect specific members of Mycobacterium tuberculosis complex in lymphocytic pleural effusion. 88 pleural effusions were cytologically examined, of which 52 were diagnosed as showing chronic inflammatory reactions. The pleural effusions were from Sudanese patients clinically suspected to have pulmonary tuberculosis. DNA was isolated using DNA extraction kits from Vivants (Malaysia). PCR Amplification of the target sequence (IS-1081 gene, 344 bp) was carried out and then analyzed using agarose gel electrophoresis. Out of the 52 studied samples of lymphocytic pleural effusions, 22 (50%) of the DNA positive samples were positive for IS-1081 gene. Molecular assays of Mycobacterium tuberculosis complex can provide rapid diagnosis of the tuberculosis which is one of the major causes of pleural effusions in general and lymphocytic effusions in specific in Sudan.},
year = {2026}
}
TY - JOUR T1 - PCR IS--1081 Gene-based Detection of Mycobacterium Tuberculosis Complex in lymphocytic Pleural Effusion Samples of Sudanese Patients AU - Hanan Babiker Eltahir AU - Mona Kamal Hosaien AU - Hind Elrayah Osman Musa AU - Ramy Yousif Hasab-Elrasul Mohammed AU - Ahmed Omar Elmubarak AU - Nazik Elmalaika Obaid Seid Ahmed Husain Y1 - 2026/01/16 PY - 2026 N1 - https://doi.org/10.11648/j.plm.20261001.11 DO - 10.11648/j.plm.20261001.11 T2 - Pathology and Laboratory Medicine JF - Pathology and Laboratory Medicine JO - Pathology and Laboratory Medicine SP - 1 EP - 4 PB - Science Publishing Group SN - 2640-4478 UR - https://doi.org/10.11648/j.plm.20261001.11 AB - Introduction: Tuberculosis (TB) has the potential to be a serious health problem in Sudan. Early laboratory confirmation of TB can lead to early initiation of treatment and increased opportunities to interrupt transmission. Nucleic acid amplification tests are widely used for detection and species differentiation of Mycobacteria in clinical specimens. This study aimed to detect specific members of Mycobacterium tuberculosis complex in lymphocytic pleural effusion. 88 pleural effusions were cytologically examined, of which 52 were diagnosed as showing chronic inflammatory reactions. The pleural effusions were from Sudanese patients clinically suspected to have pulmonary tuberculosis. DNA was isolated using DNA extraction kits from Vivants (Malaysia). PCR Amplification of the target sequence (IS-1081 gene, 344 bp) was carried out and then analyzed using agarose gel electrophoresis. Out of the 52 studied samples of lymphocytic pleural effusions, 22 (50%) of the DNA positive samples were positive for IS-1081 gene. Molecular assays of Mycobacterium tuberculosis complex can provide rapid diagnosis of the tuberculosis which is one of the major causes of pleural effusions in general and lymphocytic effusions in specific in Sudan. VL - 10 IS - 1 ER -
Faculty of Veterinary Medicine, Sudan University of Science and Technology, Hilat-Koukou, Sudan
Faculty of Medicine, Omdurman Islamic University, Omdurman, Sudan
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