When oxidation by free radicals exceeds antioxidant systems in the body, oxidative stress usually results. This is common in the underlying processes leading to aging, infertility and of a range of non-communicable diseases including cancers, cardiovascular diseases, neurodegenerative diseases and rheumatoid arthritis. There exist measures to curb oxidative stress, one of which is to take in exogenous antioxidants in form of supplements and in diet. Studies have shown that certain plants have antioxidant and free radical scavenging activity. The aim of this study is to evaluate the free radical scavenging activity (FRSA) of the crude extract of Platostoma africanum P. Beauv. (Lamiaceae). The crude extract was partitioned using N-hexane, Di-ethylether, Ethyl acetate and N-butanol in that order. TLC phytochemical screening as well as DPPH bioautographic analysis was done for each fraction as well as the residual aqueous fraction in suitable mobile phase system in order to evaluate their FRSA and secondary metabolites. A DPPH assay was then carried out using ultraviolet spectroscopy to quantify the activity. The fraction with the lowest IC50 and highest activity was further separated into fractions by column chromatography. DPPH bioautography and phytochemical screening were done on the resulting fractions. The crude as well as the five fractions had FRSA in varying degrees. Ethyl acetate fraction had the lowest IC50 (0.42mg/mL) and the highest activity. The decreasing order of activity was ethyl acetate>diethyl ether>n-butanol>crude>aqueous>n-hexane. Among the fractions obtained from column chromatography, fraction D was the most active. Terpenoids and flavonoids were the main secondary metabolites observed in the plant and were responsible for the FRSA. Platostoma africanum P. Beauv. has antioxidant activity which is mostly due to its terpenoid and flavonoid content.
| Published in | International Journal of Pharmacy and Chemistry (Volume 7, Issue 5) |
| DOI | 10.11648/j.ijpc.20210705.13 |
| Page(s) | 101-110 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Platostoma africanum P. Beauv., FRSA, Antioxidant, Phytochemical Screening, Column Chromatography, DPPH Bioautography
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APA Style
Oluwatoyin Alice Tella, Akawa Ayodeji Benjamin, Fasuba Ilesanmi Kayode, Adu Isaac Adekola, Akawa Oluwole Bidemi, et al. (2021). Free Radical Scavenging Activity of a 70% Ethanol Extract of the Whole Herb of Platostoma africanum P. Beaur. (Lamiaceae). International Journal of Pharmacy and Chemistry, 7(5), 101-110. https://doi.org/10.11648/j.ijpc.20210705.13
ACS Style
Oluwatoyin Alice Tella; Akawa Ayodeji Benjamin; Fasuba Ilesanmi Kayode; Adu Isaac Adekola; Akawa Oluwole Bidemi, et al. Free Radical Scavenging Activity of a 70% Ethanol Extract of the Whole Herb of Platostoma africanum P. Beaur. (Lamiaceae). Int. J. Pharm. Chem. 2021, 7(5), 101-110. doi: 10.11648/j.ijpc.20210705.13
AMA Style
Oluwatoyin Alice Tella, Akawa Ayodeji Benjamin, Fasuba Ilesanmi Kayode, Adu Isaac Adekola, Akawa Oluwole Bidemi, et al. Free Radical Scavenging Activity of a 70% Ethanol Extract of the Whole Herb of Platostoma africanum P. Beaur. (Lamiaceae). Int J Pharm Chem. 2021;7(5):101-110. doi: 10.11648/j.ijpc.20210705.13
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Download@article{10.11648/j.ijpc.20210705.13,
author = {Oluwatoyin Alice Tella and Akawa Ayodeji Benjamin and Fasuba Ilesanmi Kayode and Adu Isaac Adekola and Akawa Oluwole Bidemi and Olaiya Oluranti Esther},
title = {Free Radical Scavenging Activity of a 70% Ethanol Extract of the Whole Herb of Platostoma africanum P. Beaur. (Lamiaceae)},
journal = {International Journal of Pharmacy and Chemistry},
volume = {7},
number = {5},
pages = {101-110},
doi = {10.11648/j.ijpc.20210705.13},
url = {https://doi.org/10.11648/j.ijpc.20210705.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20210705.13},
abstract = {When oxidation by free radicals exceeds antioxidant systems in the body, oxidative stress usually results. This is common in the underlying processes leading to aging, infertility and of a range of non-communicable diseases including cancers, cardiovascular diseases, neurodegenerative diseases and rheumatoid arthritis. There exist measures to curb oxidative stress, one of which is to take in exogenous antioxidants in form of supplements and in diet. Studies have shown that certain plants have antioxidant and free radical scavenging activity. The aim of this study is to evaluate the free radical scavenging activity (FRSA) of the crude extract of Platostoma africanum P. Beauv. (Lamiaceae). The crude extract was partitioned using N-hexane, Di-ethylether, Ethyl acetate and N-butanol in that order. TLC phytochemical screening as well as DPPH bioautographic analysis was done for each fraction as well as the residual aqueous fraction in suitable mobile phase system in order to evaluate their FRSA and secondary metabolites. A DPPH assay was then carried out using ultraviolet spectroscopy to quantify the activity. The fraction with the lowest IC50 and highest activity was further separated into fractions by column chromatography. DPPH bioautography and phytochemical screening were done on the resulting fractions. The crude as well as the five fractions had FRSA in varying degrees. Ethyl acetate fraction had the lowest IC50 (0.42mg/mL) and the highest activity. The decreasing order of activity was ethyl acetate>diethyl ether>n-butanol>crude>aqueous>n-hexane. Among the fractions obtained from column chromatography, fraction D was the most active. Terpenoids and flavonoids were the main secondary metabolites observed in the plant and were responsible for the FRSA. Platostoma africanum P. Beauv. has antioxidant activity which is mostly due to its terpenoid and flavonoid content.},
year = {2021}
}
TY - JOUR T1 - Free Radical Scavenging Activity of a 70% Ethanol Extract of the Whole Herb of Platostoma africanum P. Beaur. (Lamiaceae) AU - Oluwatoyin Alice Tella AU - Akawa Ayodeji Benjamin AU - Fasuba Ilesanmi Kayode AU - Adu Isaac Adekola AU - Akawa Oluwole Bidemi AU - Olaiya Oluranti Esther Y1 - 2021/10/05 PY - 2021 N1 - https://doi.org/10.11648/j.ijpc.20210705.13 DO - 10.11648/j.ijpc.20210705.13 T2 - International Journal of Pharmacy and Chemistry JF - International Journal of Pharmacy and Chemistry JO - International Journal of Pharmacy and Chemistry SP - 101 EP - 110 PB - Science Publishing Group SN - 2575-5749 UR - https://doi.org/10.11648/j.ijpc.20210705.13 AB - When oxidation by free radicals exceeds antioxidant systems in the body, oxidative stress usually results. This is common in the underlying processes leading to aging, infertility and of a range of non-communicable diseases including cancers, cardiovascular diseases, neurodegenerative diseases and rheumatoid arthritis. There exist measures to curb oxidative stress, one of which is to take in exogenous antioxidants in form of supplements and in diet. Studies have shown that certain plants have antioxidant and free radical scavenging activity. The aim of this study is to evaluate the free radical scavenging activity (FRSA) of the crude extract of Platostoma africanum P. Beauv. (Lamiaceae). The crude extract was partitioned using N-hexane, Di-ethylether, Ethyl acetate and N-butanol in that order. TLC phytochemical screening as well as DPPH bioautographic analysis was done for each fraction as well as the residual aqueous fraction in suitable mobile phase system in order to evaluate their FRSA and secondary metabolites. A DPPH assay was then carried out using ultraviolet spectroscopy to quantify the activity. The fraction with the lowest IC50 and highest activity was further separated into fractions by column chromatography. DPPH bioautography and phytochemical screening were done on the resulting fractions. The crude as well as the five fractions had FRSA in varying degrees. Ethyl acetate fraction had the lowest IC50 (0.42mg/mL) and the highest activity. The decreasing order of activity was ethyl acetate>diethyl ether>n-butanol>crude>aqueous>n-hexane. Among the fractions obtained from column chromatography, fraction D was the most active. Terpenoids and flavonoids were the main secondary metabolites observed in the plant and were responsible for the FRSA. Platostoma africanum P. Beauv. has antioxidant activity which is mostly due to its terpenoid and flavonoid content. VL - 7 IS - 5 ER -
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