The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library

Huijie Liu; Yun Tian

doi:doi:10.11648/j.ijema.20190702.12

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The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library

Huijie Liu, Yun Tian

Published in International Journal of Environmental Monitoring and Analysis (Volume 7, Issue 2)

Received: 3 January 2019 Accepted: 31 January 2019 Published: 10 September 2019

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Abstract

The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.

Published in	International Journal of Environmental Monitoring and Analysis (Volume 7, Issue 2)
DOI	10.11648/j.ijema.20190702.12
Page(s)	48-55
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Mangrove Wetland, Ecological System, Microbial Diversity, 16S rDNA Library, Microbial Resources, Gene Resource

References

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[16]	Zhou Z, Meng H, Liu Y, et al. 2017. Stratified bacterial and archaeal community in mangrove and intertidal wetland mudflats revealed by high throughput 16S rRNA gene sequencing. Frontiers in Microbiology, 8, 2148.
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APA Style

Huijie Liu, Yun Tian. (2019). The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library. International Journal of Environmental Monitoring and Analysis, 7(2), 48-55. https://doi.org/10.11648/j.ijema.20190702.12

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ACS Style

Huijie Liu; Yun Tian. The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library. Int. J. Environ. Monit. Anal. 2019, 7(2), 48-55. doi: 10.11648/j.ijema.20190702.12

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AMA Style

Huijie Liu, Yun Tian. The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library. Int J Environ Monit Anal. 2019;7(2):48-55. doi: 10.11648/j.ijema.20190702.12

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@article{10.11648/j.ijema.20190702.12,
  author = {Huijie Liu and Yun Tian},
  title = {The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library},
  journal = {International Journal of Environmental Monitoring and Analysis},
  volume = {7},
  number = {2},
  pages = {48-55},
  doi = {10.11648/j.ijema.20190702.12},
  url = {https://doi.org/10.11648/j.ijema.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijema.20190702.12},
  abstract = {The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.},
 year = {2019}
}

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TY  - JOUR
T1  - The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library
AU  - Huijie Liu
AU  - Yun Tian
Y1  - 2019/09/10
PY  - 2019
N1  - https://doi.org/10.11648/j.ijema.20190702.12
DO  - 10.11648/j.ijema.20190702.12
T2  - International Journal of Environmental Monitoring and Analysis
JF  - International Journal of Environmental Monitoring and Analysis
JO  - International Journal of Environmental Monitoring and Analysis
SP  - 48
EP  - 55
PB  - Science Publishing Group
SN  - 2328-7667
UR  - https://doi.org/10.11648/j.ijema.20190702.12
AB  - The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.
VL  - 7
IS  - 2
ER  -

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Author Information

Huijie Liu

Guangzhou Military Area Environmental Monitoring Station, Guangzhou, China
Yun Tian

State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China

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APA Style

Huijie Liu, Yun Tian. (2019). The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library. International Journal of Environmental Monitoring and Analysis, 7(2), 48-55. https://doi.org/10.11648/j.ijema.20190702.12

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ACS Style

Huijie Liu; Yun Tian. The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library. Int. J. Environ. Monit. Anal. 2019, 7(2), 48-55. doi: 10.11648/j.ijema.20190702.12

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AMA Style

Huijie Liu, Yun Tian. The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library. Int J Environ Monit Anal. 2019;7(2):48-55. doi: 10.11648/j.ijema.20190702.12

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@article{10.11648/j.ijema.20190702.12,
  author = {Huijie Liu and Yun Tian},
  title = {The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library},
  journal = {International Journal of Environmental Monitoring and Analysis},
  volume = {7},
  number = {2},
  pages = {48-55},
  doi = {10.11648/j.ijema.20190702.12},
  url = {https://doi.org/10.11648/j.ijema.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijema.20190702.12},
  abstract = {The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.},
 year = {2019}
}

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TY  - JOUR
T1  - The Study of Microbial Diversity in Mangrove Wetland Ecological System Based on a 16S rDNA Library
AU  - Huijie Liu
AU  - Yun Tian
Y1  - 2019/09/10
PY  - 2019
N1  - https://doi.org/10.11648/j.ijema.20190702.12
DO  - 10.11648/j.ijema.20190702.12
T2  - International Journal of Environmental Monitoring and Analysis
JF  - International Journal of Environmental Monitoring and Analysis
JO  - International Journal of Environmental Monitoring and Analysis
SP  - 48
EP  - 55
PB  - Science Publishing Group
SN  - 2328-7667
UR  - https://doi.org/10.11648/j.ijema.20190702.12
AB  - The microbial resources in mangrove wetland ecological system are abundant because of their special characteristics. However traditional methods of isolation and culture alone cannot analyze the microbial diversity fully and so, in this study, a 16S rDNA library was constructed to analyze microbial diversity in the Fugong mangrove of the Jiulong River Estuary, Fujian Province, China. The total sediment DNA was extracted, a 16S rDNA library constructed, and the clones analyzed using the restriction fragment length polymorphism (RFLP) method. The 16S rDNA sequences of 50 clones which had a higher display frequency in the RFLP analysis were blasted with the sequences in GenBank. The results showed that the highest similarity of the sequence in clones was 100%, while the lowest was 88%. The dominant microbes from mangrove sediments in the 16S rDNA library belonged to the Proteobacteria (70%) including α-proteobacteria (6.0%), γ-proteobacteria (22.0%), δ-proteobacteria (10.0%) and ε-proteobacteria (32.0%). The other microbes were Bacteroidetes (8.0%), Planctomycetacia (2.0%), Actinobacteria (2.0%) and Verrucomicrobia (2.0%). Additional uncultured microorganisms as well as those whose classification information was unclear were also detected (16.0%). The results of this study indicated that more objective and comprehensive information of microbial diversity in mangrove wetland ecological system had been obtained. There is abundant microbial diversity and a large amount of unknown microbial resources in mangrove wetland ecological system, which could have a very important potential, and so there should be more research to explore and utilize these microbial and functional gene resources in mangrove wetland ecological system.
VL  - 7
IS  - 2
ER  -

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