Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment

Ambreen Gul; Asma Siddique; Quratulain Syed; Muhammad Nadeem; Shahjahan Baig

doi:doi:10.11648/j.ijema.20150304.15

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Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment

Ambreen Gul, Asma Siddique, Quratulain Syed, Muhammad Nadeem, Shahjahan Baig

Published in International Journal of Environmental Monitoring and Analysis (Volume 3, Issue 4)

Received: 20 February 2015 Accepted: 14 July 2015 Published: 19 August 2015

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Abstract

The High Gravity (HG) ethanol fermentation at high temperature is very attractive and promising technology for fuel ethanol production. This study was designed to improve the osmotic as well as thermal behavior of the Saccharomyces cerevisiae strain isolated from distillery waste. Therefore, initial pH and substrate concentrations were optimized for this strain. The S. cerevisiae was subjected to thermal treatment to improve its fermentation ability without significant yield losses. At pHo 5.0, 95g/L ethanol was produced with the productivity (Qp) value of 1.02. The activation energy Ea value calculated at 30-40oC was 16.48kcal/mol indicating the thermal tolerance of the strain SC36. The results of glucose optimization revealed that at 250g/L glucose concentration, Qp, Yp/s and Yp/x value of 1.53g/Lh, 0.443g/g substrate and 41.4g/g biomass were obtained. The strain’s potential to be able to ferment very high gravity medium is very promising for fuel ethanol production

Published in	International Journal of Environmental Monitoring and Analysis (Volume 3, Issue 4)
DOI	10.11648/j.ijema.20150304.15
Page(s)	233-237
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Thermal Treatment, Saccharomyces cerevisiae, Monod Model, High Gravity, Thermotolerant

References

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[2]	Bennett C (1971). Spectrophotometric acid dichromate method for the determination of ethyl alcohol. Am. J. Med. Technol. 37:217-220.
[3]	Casey GP, Magnus CA, Ingledew W (1984). High-gravity brewing: effects of nutrition on yeast composition, fermentative ability, and alcohol production. Appl. Environ.Microbiol. 48:639-646.
[4]	Dechant R, Saad S, Ibáñez AJ, Peter M (2014). Cytosolic pH regulates cell growth through distinct GTPases, Arf1 and Gtr1, to promote Ras/PKA and TORC1 activity. Mol. Cell. 55:409-421.
[5]	Dombek K, Ingram L (1987). Ethanol production during batch fermentation with Saccharomyces cerevisiae: changes in glycolytic enzymes and internal pH. Appl. Environ.Microbiol. 53:1286-1291.
[6]	Edgardo A, Carolina P, Manuel R, F J, B J (2008). Selection of thermotolerant yeast strains Saccharomyces cerevisiae for bioethanol production. Enz. Microb. Technol. 43:120-123.
[7]	Jones A, Ingledew W (1994). Fuel Alcohol Production: Optimization of Temperature for Efficient Very-High-Gravity Fermentation. Appl. Environ. Microbiol.1048-1051.
[8]	Lei J, Zhao X, Ge X, Bai F (2007). Ethanol tolerance and the variation of plasma membrane composition of yeast floc populations with different size distribution. J.Biotechnol. 131:270-275.
[9]	Leudeking RP, Edgard L (1959). A Kinetic Study of the Lactic Acid Fermentation. Batch Process at Controlled pH. J.Biochem. Microbial. Technol. 1:393-412.
[10]	Magesh A, Preetha B, Viruthagiri T (2011). Simultaneous Saccharification and Fermentation of tapioca stem var. 226 white rose to ethanol by cellulase enzyme and Saccharomyces cerevisiae. Int. J. Chem. Tech. Research. 3:1821-1829.
[11]	Miller GL (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical chemistry. 31:426-428.
[12]	Oghome P, Kamalu C (2012). Kinetics of Ethanol Production FromNypa Palm (Mangroves Palm) Through Fermentation Process.
[13]	Ohta K, Wijeyaratne SC, Hayashida S (1988). Temperature-sensitive mutants of a thermotolerant yeast, Hansenulapolymorpha. J. Ferment. Technol. 66:455-459.
[14]	Ortiz‐Muñiz B, Carvajal‐Zarrabal O, Torrestiana‐Sanchez B, Aguilar‐Uscanga MG (2010). Kinetic study on ethanol production using Saccharomyces cerevisiae ITV‐01 yeast isolated from sugar cane molasses. J. Chem. Technol.Biotechnol. 85:1361-1367.
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[16]	Piper PW (1995). The heat shock and ethanol stress responses of yeast exhibit extensive similarity and functional overlap. FEMS Microbiol. Lett. 134:121-127.
[17]	Pramanik K, Rao DE (2005). Kinetic study of ethanol fermentation of grape waste using Saccharomyces cerevisiae yeast isolated from toddy. J. Inst. Eng. 85:53-58.
[18]	Roukas T, Lazarides H, Kotzekidou P (1991). Ethanol production from deproteinized whey by Saccharomyces cerevisiae cells entrapped in different immobilization matrices. Milchwissenschaft. 46:438-441.

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APA Style

Ambreen Gul, Asma Siddique, Quratulain Syed, Muhammad Nadeem, Shahjahan Baig. (2015). Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment. International Journal of Environmental Monitoring and Analysis, 3(4), 233-237. https://doi.org/10.11648/j.ijema.20150304.15

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ACS Style

Ambreen Gul; Asma Siddique; Quratulain Syed; Muhammad Nadeem; Shahjahan Baig. Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment. Int. J. Environ. Monit. Anal. 2015, 3(4), 233-237. doi: 10.11648/j.ijema.20150304.15

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AMA Style

Ambreen Gul, Asma Siddique, Quratulain Syed, Muhammad Nadeem, Shahjahan Baig. Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment. Int J Environ Monit Anal. 2015;3(4):233-237. doi: 10.11648/j.ijema.20150304.15

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@article{10.11648/j.ijema.20150304.15,
  author = {Ambreen Gul and Asma Siddique and Quratulain Syed and Muhammad Nadeem and Shahjahan Baig},
  title = {Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment},
  journal = {International Journal of Environmental Monitoring and Analysis},
  volume = {3},
  number = {4},
  pages = {233-237},
  doi = {10.11648/j.ijema.20150304.15},
  url = {https://doi.org/10.11648/j.ijema.20150304.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijema.20150304.15},
  abstract = {The High Gravity (HG) ethanol fermentation at high temperature is very attractive and promising technology for fuel ethanol production. This study was designed to improve the osmotic as well as thermal behavior of the Saccharomyces cerevisiae strain isolated from distillery waste. Therefore, initial pH and substrate concentrations were optimized for this strain. The S. cerevisiae was subjected to thermal treatment to improve its fermentation ability without significant yield losses. At pHo 5.0, 95g/L ethanol was produced with the productivity (Qp) value of 1.02. The activation energy Ea value calculated at 30-40oC was 16.48kcal/mol indicating the thermal tolerance of the strain SC36. The results of glucose optimization revealed that at 250g/L glucose concentration, Qp, Yp/s and Yp/x value of 1.53g/Lh, 0.443g/g substrate and 41.4g/g biomass were obtained. The strain’s potential to be able to ferment very high gravity medium is very promising for fuel ethanol production},
 year = {2015}
}

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TY  - JOUR
T1  - Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment
AU  - Ambreen Gul
AU  - Asma Siddique
AU  - Quratulain Syed
AU  - Muhammad Nadeem
AU  - Shahjahan Baig
Y1  - 2015/08/19
PY  - 2015
N1  - https://doi.org/10.11648/j.ijema.20150304.15
DO  - 10.11648/j.ijema.20150304.15
T2  - International Journal of Environmental Monitoring and Analysis
JF  - International Journal of Environmental Monitoring and Analysis
JO  - International Journal of Environmental Monitoring and Analysis
SP  - 233
EP  - 237
PB  - Science Publishing Group
SN  - 2328-7667
UR  - https://doi.org/10.11648/j.ijema.20150304.15
AB  - The High Gravity (HG) ethanol fermentation at high temperature is very attractive and promising technology for fuel ethanol production. This study was designed to improve the osmotic as well as thermal behavior of the Saccharomyces cerevisiae strain isolated from distillery waste. Therefore, initial pH and substrate concentrations were optimized for this strain. The S. cerevisiae was subjected to thermal treatment to improve its fermentation ability without significant yield losses. At pHo 5.0, 95g/L ethanol was produced with the productivity (Qp) value of 1.02. The activation energy Ea value calculated at 30-40oC was 16.48kcal/mol indicating the thermal tolerance of the strain SC36. The results of glucose optimization revealed that at 250g/L glucose concentration, Qp, Yp/s and Yp/x value of 1.53g/Lh, 0.443g/g substrate and 41.4g/g biomass were obtained. The strain’s potential to be able to ferment very high gravity medium is very promising for fuel ethanol production
VL  - 3
IS  - 4
ER  -

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Author Information

Ambreen Gul

Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan
Asma Siddique

Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan
Quratulain Syed

Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan
Muhammad Nadeem

Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan
Shahjahan Baig

Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan

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Plain Text BibTeX RIS

APA Style

Ambreen Gul, Asma Siddique, Quratulain Syed, Muhammad Nadeem, Shahjahan Baig. (2015). Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment. International Journal of Environmental Monitoring and Analysis, 3(4), 233-237. https://doi.org/10.11648/j.ijema.20150304.15

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ACS Style

Ambreen Gul; Asma Siddique; Quratulain Syed; Muhammad Nadeem; Shahjahan Baig. Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment. Int. J. Environ. Monit. Anal. 2015, 3(4), 233-237. doi: 10.11648/j.ijema.20150304.15

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AMA Style

Ambreen Gul, Asma Siddique, Quratulain Syed, Muhammad Nadeem, Shahjahan Baig. Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment. Int J Environ Monit Anal. 2015;3(4):233-237. doi: 10.11648/j.ijema.20150304.15

Copy | Download

@article{10.11648/j.ijema.20150304.15,
  author = {Ambreen Gul and Asma Siddique and Quratulain Syed and Muhammad Nadeem and Shahjahan Baig},
  title = {Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment},
  journal = {International Journal of Environmental Monitoring and Analysis},
  volume = {3},
  number = {4},
  pages = {233-237},
  doi = {10.11648/j.ijema.20150304.15},
  url = {https://doi.org/10.11648/j.ijema.20150304.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijema.20150304.15},
  abstract = {The High Gravity (HG) ethanol fermentation at high temperature is very attractive and promising technology for fuel ethanol production. This study was designed to improve the osmotic as well as thermal behavior of the Saccharomyces cerevisiae strain isolated from distillery waste. Therefore, initial pH and substrate concentrations were optimized for this strain. The S. cerevisiae was subjected to thermal treatment to improve its fermentation ability without significant yield losses. At pHo 5.0, 95g/L ethanol was produced with the productivity (Qp) value of 1.02. The activation energy Ea value calculated at 30-40oC was 16.48kcal/mol indicating the thermal tolerance of the strain SC36. The results of glucose optimization revealed that at 250g/L glucose concentration, Qp, Yp/s and Yp/x value of 1.53g/Lh, 0.443g/g substrate and 41.4g/g biomass were obtained. The strain’s potential to be able to ferment very high gravity medium is very promising for fuel ethanol production},
 year = {2015}
}

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TY  - JOUR
T1  - Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment
AU  - Ambreen Gul
AU  - Asma Siddique
AU  - Quratulain Syed
AU  - Muhammad Nadeem
AU  - Shahjahan Baig
Y1  - 2015/08/19
PY  - 2015
N1  - https://doi.org/10.11648/j.ijema.20150304.15
DO  - 10.11648/j.ijema.20150304.15
T2  - International Journal of Environmental Monitoring and Analysis
JF  - International Journal of Environmental Monitoring and Analysis
JO  - International Journal of Environmental Monitoring and Analysis
SP  - 233
EP  - 237
PB  - Science Publishing Group
SN  - 2328-7667
UR  - https://doi.org/10.11648/j.ijema.20150304.15
AB  - The High Gravity (HG) ethanol fermentation at high temperature is very attractive and promising technology for fuel ethanol production. This study was designed to improve the osmotic as well as thermal behavior of the Saccharomyces cerevisiae strain isolated from distillery waste. Therefore, initial pH and substrate concentrations were optimized for this strain. The S. cerevisiae was subjected to thermal treatment to improve its fermentation ability without significant yield losses. At pHo 5.0, 95g/L ethanol was produced with the productivity (Qp) value of 1.02. The activation energy Ea value calculated at 30-40oC was 16.48kcal/mol indicating the thermal tolerance of the strain SC36. The results of glucose optimization revealed that at 250g/L glucose concentration, Qp, Yp/s and Yp/x value of 1.53g/Lh, 0.443g/g substrate and 41.4g/g biomass were obtained. The strain’s potential to be able to ferment very high gravity medium is very promising for fuel ethanol production
VL  - 3
IS  - 4
ER  -

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