Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis

Baneen Adill Mohammed Ali; Dhamiaa Makii Hamza; Dhamiaa Makii Hamza

doi:doi:10.11648/j.ijbbmb.20210602.12

| Peer-Reviewed

Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis

Baneen Adill Mohammed Ali, Dhamiaa Makii Hamza, Dhamiaa Makii Hamza

Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 6, Issue 2)

Received: 21 June 2021 Accepted: 13 August 2021 Published: 5 October 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Toxoplasma.gondii is a protozoan eukaryotic parasite that can enter the body host as an obligate intracellular parasite and causes toxoplasmosis infection, it has the ability to cause latent infection in the host tissue such as cardiac muscle, skeletal muscles, or the central nervous system Aim of study: Determine the relationship between the parasitic infection of T. gondii and Programmed cell death-1 (PD-1), TGF- ß and IL-10 during abortion. Materials/Methods: One hundred consecutive samples after abortion were collected. The placental tissue samples were put in formalin for immunohistochemistry technique. Result the result of this study reveals that Residence did not have a relation with the presence or absence of Toxoplasmosis (P>0.05), age was significantly different between Toxoplasmosis groups (P<0.05), Number of abortions was not significantly different between the Toxoplasmosis group (P>0.05), PDL-1, IL-10, and TGF-β levels and their relationship during infection is significant (P<0.05). Conclusion the PDL-1, IL-10, and TGF-β levels could be a candidate as a biomarker for the pathological effect of Toxoplasma.gondiin during pregnancy.

Published in	International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 6, Issue 2)
DOI	10.11648/j.ijbbmb.20210602.12
Page(s)	34-41
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Toxoplasma Gondiin, PDL-1, IL-10, TGF-β

References

[1]	Abdoli, A., Maspi, N. and Ghaffarifar, F. (2017). Wound healing in cutaneous leishmaniasis: A double edged sword of IL-10 and TGF-β. Comparative Immunology, Microbiology and Infectious Diseases, [online] 51, pp. 15–26.
[2]	Abdulkhaliq, R. J., Mohammed, S. T. and Abbas, A. A.-H. (2018). The Role of IL-6 and TGF-β1 in Iraqi Women with Recurrent Abortion. ufds.uofallujah.edu.iq.
[3]	Agmas, B., Tesfaye, R. and Koye, D. N. (2015). Seroprevalence of Toxoplasma gondii infection and associated risk factors among pregnant women in Debre Tabor, Northwest Ethiopia. BMC Research Notes, 8 (1).
[4]	Aldabagh, M.-G. H., Hachim, S., Qassim, K., Al-Mayah, Q., Hassan, J. and Salloom, D. (2018). Immune profile in aborted Iraqi women with toxoplasmosis. Medical Journal of Babylon, 15 (1), p. 48.
[5]	Alijotas-Reig, J., Llurba, E. and Gris, J. Ma. (2014). potentiating maternal immune tolerance in pregnancy: A new challenging role for regulatory T cells. Placenta, 35 (4), pp. 241–248.
[6]	Bamba, S., Cissé, M., Sangaré, I., Zida, A., Ouattara, S. and Guiguemdé, R. T. (2017). Seroprevalence and risk factors of Toxoplasma gondii infection in pregnant women from Bobo Dioulasso, Burkina Faso. BMC Infectious Diseases, 17 (1).
[7]	Barroso, D. H., Falcão, S. D. A. C., Motta, J. de O. C. da, Sevilha dos Santos, L., Takano, G. H. S., Gomes, C. M., Favali, C. B. F., de Lima, B. D. and Sampaio, R. N. R. (2018). PD-L1 May Mediate T-Cell Exhaustion in a Case of Early Diffuse Leishmaniasis Caused by Leishmania (L.) amazonensis. Frontiers in Immunology, 9.
[8]	da Fonseca-Martins, A. M., Ramos, T. D., Pratti, J. E. S., Firmino-Cruz, L., Gomes, D. C. O., Soong, L., Saraiva, E. M. and de Matos Guedes, H. L. (2019). Protection induced by anti-PD-1 and anti-PD-L1 treatment inLeishmania amazonensis-infected BALB/c mice.
[9]	El-Sherbini, M. S. et al. (2019) ‘Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cells’, Egyptian Journal of Medical Human Genetics, 20 (1), pp. 0–9. doi: 10.1186/s43042-019-0006-5.
[10]	Fitri, L. E., Sardjono, T. W., Rahmah, Z., Siswanto, B., Handono, K. and Dachlan, Y. P. (2015). Low Fetal Weight is Directly Caused by Sequestration of Parasites and Indirectly by IL-17 and IL-10 Imbalance in the Placenta of Pregnant Mice with Malaria. The Korean Journal of Parasitology, 53 (2), pp. 189–196.
[11]	Gelaye, W., Kebede, T. and Hailu, A. (2015). High prevalence of anti-toxoplasma antibodies and absence of Toxoplasma gondii infection risk factors among pregnant women attending routine antenatal care in two Hospitals of Addis Ababa, Ethiopia. International Journal of Infectious Diseases, 34, pp. 41–45.
[12]	Habib, S., El Andaloussi, A., Elmasry, K., Handoussa, A., Azab, M., Elsawey, A., Al-Hendy, A. and Ismail, N. (2018). PDL-1 Blockade Prevents T Cell Exhaustion, Inhibits Autophagy, and Promotes Clearance of Leishmania donovani. Infection and Immunity, [online] 86 (6).
[13]	Habicht, A., Dada, S., Jurewicz, M., Fife, B. T., Yagita, H., Azuma, M., Sayegh, M. H. and Guleria, I. (2007). A Link between PDL1 and T Regulatory Cells in Fetomaternal Tolerance. The Journal of Immunology, 179 (8), pp. 5211–5219.
[14]	Huang, N., Chi, H. and Qiao, J. (2020). Role of Regulatory T Cells in Regulating Fetal-Maternal Immune Tolerance in Healthy Pregnancies and Reproductive Diseases. Frontiers in Immunology, 11.
[15]	Jones, J. L., Kruszon-Moran, D., Elder, S., Rivera, H. N., Press, C., Montoya, J. G. and McQuillan, G. M. (2017). Toxoplasma gondii Infection in the United States, 2011–2014. The American Journal of Tropical Medicine and Hygiene, [online] 98 (2), pp. 551–557.
[16]	Kumar, R., Chauhan, S. B., Ng, S. S., Sundar, S. and Engwerda, C. R. (2017). Immune Checkpoint Targets for Host-Directed Therapy to Prevent and Treat Leishmaniasis. Frontiers in Immunology, 8.
[17]	Liu, X., Jiang, M., Ren, L., Zhang, A., Zhao, M., Zhang, H., Jiang, Y. and Hu, X. (2018). Decidual macrophage M1 polarization contributes to adverse pregnancy induced by Toxoplasma gondii PRU strain infection. Microbial Pathogenesis, 124, pp. 183–190.
[18]	Liu, Y., Zhao, M., Xu, X., Liu, X., Zhang, H., Jiang, Y., Zhang, L. and Hu, X. (2014). Adoptive Transfer of Treg Cells Counters Adverse Effects of Toxoplasma gondii Infection on Pregnancy. Journal of Infectious Diseases, 210 (9), pp. 1435–1443.
[19]	Magdoud, K., Granados Herbepin, V., Messaoudi, S., Hizem, S., Bouafia, N., Almawi, W. Y., Mahjoub, T. and Touraine, R. (2013). Genetic variation in TGFB1 gene and risk of idiopathic recurrent pregnancy loss. MHR: Basic science of reproductive medicine, 19 (7), pp. 438–443.
[20]	Majid, A., Khan, S., Jan, A. H., Taib, M., Adnan, M., Ali, I. and Khan, S. N. (2016). Chronic toxoplasmosis and possible risk factors associated with pregnant women in Khyber Pakhtunkhwa. Biotechnology & Biotechnological Equipment, 30 (4), pp. 733–736.
[21]	Mendez, O. A. and Koshy, A. A. (2017) ‘Toxoplasma gondii: Entry, association, and physiological influence on the central nervous system’, PLoS Pathogens, 13 (7), pp. 1–12. doi: 10.1371/journal.ppat.1006351.
[22]	Milne, G., Fujimoto, C., Bean, T., Peters, H. J., Hemmington, M., Taylor, C., Fowkes, R. C., Martineau, H. M., Hamilton, C. M., Walker, M., Mitchell, J. A., Léger, E., Priestnall, S. L. and Webster, J. P. (2020). Infectious Causation of Abnormal Host Behavior: Toxoplasma gondii and Its Potential Association with Dopey Fox Syndrome. Frontiers in Psychiatry, 11.
[23]	Morelli, S. S., Mandal, M., Goldsmith, L. T., Kashani, B. N. and Ponzio, N. M. (2015). The maternal immune system during pregnancy and its influence on fetal development. [online] Research and Reports in Biology.
[24]	Olariu, T. R., Ursoniu, S., Hotea, I., Dumitrascu, V., Anastasiu, D. and Lupu, M. A. (2020). Seroprevalence and Risk Factors of Toxoplasma gondii Infection in Pregnant Women from Western Romania. Vector-Borne and Zoonotic Diseases, 20 (10), pp. 763–767.
[25]	Piccinni, M.-P., Lombardelli, L., Logiodice, F., Kullolli, O., Romagnani, S. and Le Bouteiller, P. (2015). T helper cell mediated-tolerance towards fetal allograft in successful pregnancy. Clinical and Molecular Allergy, 13 (1).
[26]	Rehman, F., Shah, M., Ali, A., Rapisarda, A. M. C. and Cianci, A. (2020). Seroprevalence and risk factors of Toxoplasma gondii infection in women with recurrent fetal loss from the province of Khyber Pakhtunkhwa, Pakistan. Journal of Neonatal-Perinatal Medicine, pp. 1–7.
[27]	Rostami, A., Riahi, S. M., Contopoulos-Ioannidis, D. G., Gamble, H. R., Fakhri, Y., Shiadeh, M. N., Foroutan, M., Behniafar, H., Taghipour, A., Maldonado, Y. A., Mokdad, A. H. and Gasser, R. B. (2019). Acute Toxoplasma infection in pregnant women worldwide: A systematic review and meta-analysis. PLOS Neglected Tropical Diseases, 13 (10), p. e0007807.
[28]	Schönrich, G. and Raftery, M. J. (2019) ‘The PD-1/PD-L1 axis and virus infections: A delicate balance’, Frontiers in Cellular and Infection Microbiology, 9 (JUN). doi: 10.3389/fcimb.2019.00207.
[29]	Scorza, B., Carvalho, E. and Wilson, M. (2017). Cutaneous Manifestations of Human and Murine Leishmaniasis. International Journal of Molecular Sciences, 18 (6), p. 1296.
[30]	Shapiro, K., Bahia-Oliveira, L., Dixon, B., Dumètre, A., de Wit, L. A., VanWormer, E. and Villena, I. (2019). Environmental transmission of Toxoplasma gondii: Oocysts in water, soil and food. Food and Waterborne Parasitology.
[31]	Song, S., Yuan, P., Wu, H., Chen, J., Fu, J., Li, P., Lu, J. and Wei, W. (2014). Dendritic cells with an increased PD-L1 by TGF-β induce T cell anergy for the cytotoxicity of hepatocellular carcinoma cells. International Immunopharmacology, 20 (1), pp. 117–123.
[32]	Toepp, A. J. and Petersen, C. A. (2020). The balancing act: Immunology of leishmaniosis. Research in Veterinary Science, 130, pp. 19–25.
[33]	Wam, E. C., Sama, L. F., Ali, I. M., Ebile, W. A., Aghangu, L. A. and Tume, C. B. (2016). Seroprevalence of Toxoplasma gondii IgG and IgM antibodies and associated risk factors in women of child-bearing age in Njinikom, NW Cameroon. BMC Research Notes, 9 (1).
[34]	Wang, J., Jebbawi, F., Bellanger, A.-P., Beldi, G., Millon, L. and Gottstein, B. (2018). Immunotherapy of alveolar echinococcosis via PD-1/PD-L1 immune checkpoint blockade in mice. Parasite Immunology, 40 (12), p. e12596.
[35]	Wilking, H., Thamm, M., Stark, K., Aebischer, T. and Seeber, F. (2016). Prevalence, incidence estimations and risk factors of Toxoplasma gondii infection in Germany: a representative, cross-sectional, serological study. Scientific Reports, 6 (1).
[36]	Zare-Bidaki, M., Assar, S., Hakimi, H., Abdollahi, S. H., Nosratabadi, R., Kennedy, D. and Arababadi, M. K. (2016). TGF-β in Toxoplasmosis: Friend or foe? Cytokine, 86, pp. 29–35.
[37]	Zeng, W., Qin, S., Wang, R., Zhang, Y., Ma, X., Tian, F., Liu, X.-R., Qin, X., Liao, S., Sun, L. and Lin, Y. (2020). PDL1 blockage increases fetal resorption and Tfr cells but does not affect Tfh/Tfr ratio and B-cell maturation during allogeneic pregnancy. Cell Death & Disease, [online] 11 (2), pp. 1–13.
[38]	Zhao, M., Zhang, H., Liu, X., Jiang, Y., Ren, L. and Hu, X. (2017). The Effect of TGF-β on Treg Cells in Adverse Pregnancy Outcome upon Toxoplasma gondii Infection. Frontiers in Microbiology, 8.
[39]	Analysis of cytokines IFN-γ, TNF-α, TGF-β and nitric oxide in amniotic fluid and serum of pregnant women with toxoplasmosis in southern Brazil. (2018). Cytokine, [online] 106, pp. 35–39.

Cite This Article

Plain Text BibTeX RIS

APA Style

Baneen Adill Mohammed Ali, Dhamiaa Makii Hamza, Dhamiaa Makii Hamza. (2021). Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis. International Journal of Biochemistry, Biophysics & Molecular Biology, 6(2), 34-41. https://doi.org/10.11648/j.ijbbmb.20210602.12

Copy | Download

ACS Style

Baneen Adill Mohammed Ali; Dhamiaa Makii Hamza; Dhamiaa Makii Hamza. Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis. Int. J. Biochem. Biophys. Mol. Biol. 2021, 6(2), 34-41. doi: 10.11648/j.ijbbmb.20210602.12

Copy | Download

AMA Style

Baneen Adill Mohammed Ali, Dhamiaa Makii Hamza, Dhamiaa Makii Hamza. Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis. Int J Biochem Biophys Mol Biol. 2021;6(2):34-41. doi: 10.11648/j.ijbbmb.20210602.12

Copy | Download

@article{10.11648/j.ijbbmb.20210602.12,
  author = {Baneen Adill Mohammed Ali and Dhamiaa Makii Hamza and Dhamiaa Makii Hamza},
  title = {Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {6},
  number = {2},
  pages = {34-41},
  doi = {10.11648/j.ijbbmb.20210602.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20210602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20210602.12},
  abstract = {Toxoplasma.gondii is a protozoan eukaryotic parasite that can enter the body host as an obligate intracellular parasite and causes toxoplasmosis infection, it has the ability to cause latent infection in the host tissue such as cardiac muscle, skeletal muscles, or the central nervous system Aim of study: Determine the relationship between the parasitic infection of T. gondii and Programmed cell death-1 (PD-1), TGF- ß and IL-10 during abortion. Materials/Methods: One hundred consecutive samples after abortion were collected. The placental tissue samples were put in formalin for immunohistochemistry technique. Result the result of this study reveals that Residence did not have a relation with the presence or absence of Toxoplasmosis (P>0.05), age was significantly different between Toxoplasmosis groups (P0.05), PDL-1, IL-10, and TGF-β levels and their relationship during infection is significant (PToxoplasma.gondiin during pregnancy.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis
AU  - Baneen Adill Mohammed Ali
AU  - Dhamiaa Makii Hamza
AU  - Dhamiaa Makii Hamza
Y1  - 2021/10/05
PY  - 2021
N1  - https://doi.org/10.11648/j.ijbbmb.20210602.12
DO  - 10.11648/j.ijbbmb.20210602.12
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 34
EP  - 41
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20210602.12
AB  - Toxoplasma.gondii is a protozoan eukaryotic parasite that can enter the body host as an obligate intracellular parasite and causes toxoplasmosis infection, it has the ability to cause latent infection in the host tissue such as cardiac muscle, skeletal muscles, or the central nervous system Aim of study: Determine the relationship between the parasitic infection of T. gondii and Programmed cell death-1 (PD-1), TGF- ß and IL-10 during abortion. Materials/Methods: One hundred consecutive samples after abortion were collected. The placental tissue samples were put in formalin for immunohistochemistry technique. Result the result of this study reveals that Residence did not have a relation with the presence or absence of Toxoplasmosis (P>0.05), age was significantly different between Toxoplasmosis groups (P0.05), PDL-1, IL-10, and TGF-β levels and their relationship during infection is significant (PToxoplasma.gondiin during pregnancy.
VL  - 6
IS  - 2
ER  -

Copy | Download

Author Information

Baneen Adill Mohammed Ali

College of Medicine, University of Kerbala, Kerbala, Iraq
Dhamiaa Makii Hamza

College of Medicine, University of Kerbala, Kerbala, Iraq
Dhamiaa Makii Hamza

College of Medicine, University of Kerbala, Kerbala, Iraq

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Baneen Adill Mohammed Ali, Dhamiaa Makii Hamza, Dhamiaa Makii Hamza. (2021). Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis. International Journal of Biochemistry, Biophysics & Molecular Biology, 6(2), 34-41. https://doi.org/10.11648/j.ijbbmb.20210602.12

Copy | Download

ACS Style

Baneen Adill Mohammed Ali; Dhamiaa Makii Hamza; Dhamiaa Makii Hamza. Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis. Int. J. Biochem. Biophys. Mol. Biol. 2021, 6(2), 34-41. doi: 10.11648/j.ijbbmb.20210602.12

Copy | Download

AMA Style

Baneen Adill Mohammed Ali, Dhamiaa Makii Hamza, Dhamiaa Makii Hamza. Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis. Int J Biochem Biophys Mol Biol. 2021;6(2):34-41. doi: 10.11648/j.ijbbmb.20210602.12

Copy | Download

@article{10.11648/j.ijbbmb.20210602.12,
  author = {Baneen Adill Mohammed Ali and Dhamiaa Makii Hamza and Dhamiaa Makii Hamza},
  title = {Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {6},
  number = {2},
  pages = {34-41},
  doi = {10.11648/j.ijbbmb.20210602.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20210602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20210602.12},
  abstract = {Toxoplasma.gondii is a protozoan eukaryotic parasite that can enter the body host as an obligate intracellular parasite and causes toxoplasmosis infection, it has the ability to cause latent infection in the host tissue such as cardiac muscle, skeletal muscles, or the central nervous system Aim of study: Determine the relationship between the parasitic infection of T. gondii and Programmed cell death-1 (PD-1), TGF- ß and IL-10 during abortion. Materials/Methods: One hundred consecutive samples after abortion were collected. The placental tissue samples were put in formalin for immunohistochemistry technique. Result the result of this study reveals that Residence did not have a relation with the presence or absence of Toxoplasmosis (P>0.05), age was significantly different between Toxoplasmosis groups (P0.05), PDL-1, IL-10, and TGF-β levels and their relationship during infection is significant (PToxoplasma.gondiin during pregnancy.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Study Levels of PDL-1, IL-10 and TGF-β in Patients with Toxoplasmosis
AU  - Baneen Adill Mohammed Ali
AU  - Dhamiaa Makii Hamza
AU  - Dhamiaa Makii Hamza
Y1  - 2021/10/05
PY  - 2021
N1  - https://doi.org/10.11648/j.ijbbmb.20210602.12
DO  - 10.11648/j.ijbbmb.20210602.12
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 34
EP  - 41
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20210602.12
AB  - Toxoplasma.gondii is a protozoan eukaryotic parasite that can enter the body host as an obligate intracellular parasite and causes toxoplasmosis infection, it has the ability to cause latent infection in the host tissue such as cardiac muscle, skeletal muscles, or the central nervous system Aim of study: Determine the relationship between the parasitic infection of T. gondii and Programmed cell death-1 (PD-1), TGF- ß and IL-10 during abortion. Materials/Methods: One hundred consecutive samples after abortion were collected. The placental tissue samples were put in formalin for immunohistochemistry technique. Result the result of this study reveals that Residence did not have a relation with the presence or absence of Toxoplasmosis (P>0.05), age was significantly different between Toxoplasmosis groups (P0.05), PDL-1, IL-10, and TGF-β levels and their relationship during infection is significant (PToxoplasma.gondiin during pregnancy.
VL  - 6
IS  - 2
ER  -

Copy | Download